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Union","award":["RRF-2.3.1-21-2022-00015"],"award-info":[{"award-number":["RRF-2.3.1-21-2022-00015"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceutics"],"abstract":"<jats:p>The application of enzyme-based therapies has received significant attention in modern drug development. Lipases are one of the most versatile enzymes that can be used as therapeutic agents in basic skin care and medical treatment related to excessive sebum production, acne, and inflammation. The traditional formulations available for skin treatment, such as creams, ointments or gels, are widely applied; however, their use is not always accompanied by good drug penetration properties, stability, or patient adherence. Nanoformulated drugs offer the possibility of combining enzymatic and small molecule formulations, making them a new and exciting alternative in this field. In this study polymeric nanofibrous matrices made of polyvinylpyrrolidone and polylactic acid were developed, entrapping lipases from Candida rugosa and Rizomucor miehei and antibiotic compound nadifloxacin. The effect of the type of polymers and lipases were investigated, and the nanofiber formation process was optimized to provide a promising alternative in topical treatment. Our experiments have shown that entrapment by electrospinning induced two orders of magnitude increase in the specific enzyme activity of lipases. Permeability investigations indicated that all lipase-loaded nanofibrous masks were capable of delivering nadifloxacin to the human epidermis, confirming the viability of electrospinning as a formulation method for topical skin medications.<\/jats:p>","DOI":"10.3390\/pharmaceutics15041174","type":"journal-article","created":{"date-parts":[[2023,4,7]],"date-time":"2023-04-07T04:04:16Z","timestamp":1680840256000},"page":"1174","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Combined Nanofibrous Face Mask: Co-Formulation of Lipases and Antibiotic Agent by Electrospinning Technique"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9957-1203","authenticated-orcid":false,"given":"Di\u00e1na","family":"Balogh-Weiser","sequence":"first","affiliation":[{"name":"Department of Organic Chemistry and Technology, Budapest University of Technology and Economics, M\u0171egyetem rkp. 3, H-1111 Budapest, Hungary"},{"name":"Department of Physical Chemistry and Materials Science, Budapest University of Technology and Economics, M\u0171egyetem rkp. 3, H-1111 Budapest, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Moln\u00e1r","sequence":"additional","affiliation":[{"name":"Department of Organic Chemistry and Technology, Budapest University of Technology and Economics, M\u0171egyetem rkp. 3, H-1111 Budapest, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerg\u0151 D.","family":"T\u00f3th","sequence":"additional","affiliation":[{"name":"Department of Physical Chemistry and Materials Science, Budapest University of Technology and Economics, M\u0171egyetem rkp. 3, H-1111 Budapest, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3791-1057","authenticated-orcid":false,"given":"G\u00e1bor","family":"Kopl\u00e1nyi","sequence":"additional","affiliation":[{"name":"Department of Organic Chemistry and Technology, Budapest University of Technology and Economics, M\u0171egyetem rkp. 3, H-1111 Budapest, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f3zsef","family":"Szemes","sequence":"additional","affiliation":[{"name":"Department of Organic Chemistry and Technology, Budapest University of Technology and Economics, M\u0171egyetem rkp. 3, H-1111 Budapest, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bal\u00e1zs","family":"Decsi","sequence":"additional","affiliation":[{"name":"Department of Organic Chemistry and Technology, Budapest University of Technology and Economics, M\u0171egyetem rkp. 3, H-1111 Budapest, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1564-4813","authenticated-orcid":false,"given":"G\u00e1bor","family":"Katona","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutical Technology and Regulatory Affairs, University of Szeged, E\u00f6tv\u00f6s u. 6, H-6720 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maryana","family":"Salamah","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutical Technology and Regulatory Affairs, University of Szeged, E\u00f6tv\u00f6s u. 6, H-6720 Szeged, Hungary"},{"name":"Istitute of Pharmacodynamics and Biopharmacy, Faculty of Pharmacy, University of Szeged, E\u00f6tv\u00f6s u. 6, H-6720 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ferenc","family":"Ender","sequence":"additional","affiliation":[{"name":"Department of Electron Devices, Budapest University of Technology and Economics, M\u0171egyetem rkp. 3, H-1111 Budapest, Hungary"},{"name":"SpinSplit LLC, Vend u. 17, H-1025 Budapest, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5593-1329","authenticated-orcid":false,"given":"Anita","family":"Kov\u00e1cs","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutical Technology and Regulatory Affairs, University of Szeged, E\u00f6tv\u00f6s u. 6, H-6720 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3842-8876","authenticated-orcid":false,"given":"Szilvia","family":"Berk\u00f3","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutical Technology and Regulatory Affairs, University of Szeged, E\u00f6tv\u00f6s u. 6, H-6720 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5187-5702","authenticated-orcid":false,"given":"M\u00e1ria","family":"Budai-Sz\u0171cs","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutical Technology and Regulatory Affairs, University of Szeged, E\u00f6tv\u00f6s u. 6, H-6720 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gy\u00f6rgy T.","family":"Balogh","sequence":"additional","affiliation":[{"name":"Istitute of Pharmacodynamics and Biopharmacy, Faculty of Pharmacy, University of Szeged, E\u00f6tv\u00f6s u. 6, H-6720 Szeged, Hungary"},{"name":"Department of Chemical and Environmental Process Engineering, Budapest University of Technology and Economics, M\u0171egyetem rkp. 3, H-1111 Budapest, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Punekar, N.S. 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