{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T15:33:43Z","timestamp":1787499223008,"version":"build-2736575974"},"reference-count":74,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2023,4,29]],"date-time":"2023-04-29T00:00:00Z","timestamp":1682726400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Serbian Ministry of Science, Technological Development and Innovation","award":["451-03-47\/2023-01\/200003"],"award-info":[{"award-number":["451-03-47\/2023-01\/200003"]}]},{"name":"Serbian Ministry of Science, Technological Development and Innovation","award":["451-03-47\/2023-01\/200113"],"award-info":[{"award-number":["451-03-47\/2023-01\/200113"]}]},{"name":"Serbian Ministry of Science, Technological Development and Innovation","award":["68"],"award-info":[{"award-number":["68"]}]},{"name":"Internal Project of the Faculty of Medicine, University of Ni\u0161, Serbia","award":["451-03-47\/2023-01\/200003"],"award-info":[{"award-number":["451-03-47\/2023-01\/200003"]}]},{"name":"Internal Project of the Faculty of Medicine, University of Ni\u0161, Serbia","award":["451-03-47\/2023-01\/200113"],"award-info":[{"award-number":["451-03-47\/2023-01\/200113"]}]},{"name":"Internal Project of the Faculty of Medicine, University of Ni\u0161, Serbia","award":["68"],"award-info":[{"award-number":["68"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceutics"],"abstract":"<jats:p>The use of medicinal plant species and their products is widespread in the field of gastrointestinal and respiratory diseases. This study aimed to evaluate the traditional use of Salvia sclarea L., clary sage, finding the possible mechanisms of its spasmolytic and bronchodilator actions in in vitro conditions supported by molecular docking analysis, along with the antimicrobial effects. Four dry extracts were prepared from the aerial parts of S. sclarea, using absolute or 80% (v\/v) methanol by the method of a single-stage maceration or an ultrasound-assisted extraction. Characterization of the bioactive compounds by high-performance liquid chromatography indicated the presence of significant amounts of polyphenolics, with rosmarinic acid as the prevalent one. The spontaneous ileal contractions were best inhibited by the extract prepared with 80% methanol and maceration. The same extract was superior in the carbachol- and KCl-induced tracheal smooth muscle contractions, being the strongest bronchodilator agent. The most powerful relaxation of KCl-induced ileal contractions was achieved with the extract made of absolute methanol by maceration, while the 80% methanolic extract made with the ultrasound method generated the best spasmolytic effects in the acetylcholine-induced ileal contractions. Docking analysis suggested that apigenin-7-O-glucoside and luteolin-7-O-glucoside exhibited the highest binding affinity to voltage-gated calcium channels. Gram (+) bacteria were more susceptible to the effects of the extracts, particularly Staphylococcus aureus, in contrast to Gram (\u2212) bacteria and Candida albicans. This is the first study to point out the influence of S. sclarea methanolic extracts on the gastrointestinal and respiratory spasm reduction, paving the way for their potential place in complementary medicine.<\/jats:p>","DOI":"10.3390\/pharmaceutics15051376","type":"journal-article","created":{"date-parts":[[2023,5,1]],"date-time":"2023-05-01T12:02:31Z","timestamp":1682942551000},"page":"1376","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["An In Vitro and In Silico Characterization of Salvia sclarea L. Methanolic Extracts as Spasmolytic Agents"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6951-9957","authenticated-orcid":false,"given":"Milica","family":"Randjelovi\u0107","sequence":"first","affiliation":[{"name":"Department of Pharmacy, Faculty of Medicine, University of Ni\u0161, Blvd. Dr Zorana Djindji\u0107a 81, 18000 Ni\u0161, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suzana","family":"Brankovi\u0107","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Medicine, University of Ni\u0161, Blvd. Dr Zorana Djindji\u0107a 81, 18000 Ni\u0161, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0039-0929","authenticated-orcid":false,"given":"Milo\u0161","family":"Jovanovi\u0107","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Faculty of Medicine, University of Ni\u0161, Blvd. Dr Zorana Djindji\u0107a 81, 18000 Ni\u0161, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nemanja","family":"Kiti\u0107","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, University of Ni\u0161, Blvd. Dr Zorana Djindji\u0107a 81, 18000 Ni\u0161, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Slavoljub","family":"\u017divanovi\u0107","sequence":"additional","affiliation":[{"name":"Research Center for Biomedicine, Faculty of Medicine, University of Ni\u0161, Blvd. Dr Zorana Djindji\u0107a 81, 18000 Ni\u0161, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatjana","family":"Mihajilov-Krstev","sequence":"additional","affiliation":[{"name":"Department of Biology and Ecology, Faculty of Science and Mathematics, University of Nis, Vi\u0161egradska 33, 18000 Ni\u0161, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bojana","family":"Miladinovi\u0107","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Faculty of Medicine, University of Ni\u0161, Blvd. Dr Zorana Djindji\u0107a 81, 18000 Ni\u0161, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milica","family":"Milutinovi\u0107","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Faculty of Medicine, University of Ni\u0161, Blvd. Dr Zorana Djindji\u0107a 81, 18000 Ni\u0161, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Du\u0161anka","family":"Kiti\u0107","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Faculty of Medicine, University of Ni\u0161, Blvd. Dr Zorana Djindji\u0107a 81, 18000 Ni\u0161, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Palhares, R.M., Drummond, M.G., Brasil, B.D.S.A.F., Cosenza, G.P., Brand\u00e3o, M.D.G.L., and Oliveira, G. 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