{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T00:49:18Z","timestamp":1792111758827,"version":"4.4.0"},"reference-count":56,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2023,5,27]],"date-time":"2023-05-27T00:00:00Z","timestamp":1685145600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Walloon Public Service, BIOWIN (Health Cluster of Wallonia, Belgium)"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceutics"],"abstract":"<jats:p>Phage therapy is recognized to be a promising alternative to fight antibiotic-resistant infections. In the quest for oral dosage forms containing bacteriophages, the utilization of colonic-release Eudragit\u00ae derivatives has shown potential in shielding bacteriophages from the challenges encountered within the gastrointestinal tract, such as fluctuating pH levels and the presence of digestive enzymes. Consequently, this study aimed to develop targeted oral delivery systems for bacteriophages, specifically focusing on colon delivery and employing Eudragit\u00ae FS30D as the excipient. The bacteriophage model used was LUZ19. An optimized formulation was established to not only preserve the activity of LUZ19 during the manufacturing process but also ensure its protection from highly acidic conditions. Flowability assessments were conducted for both capsule filling and tableting processes. Furthermore, the viability of the bacteriophages remained unaffected by the tableting process. Additionally, the release of LUZ19 from the developed system was evaluated using the Simulator of the Human Intestinal Microbial Ecosystem (SHIME\u00ae) model. Finally, stability studies demonstrated that the powder remained stable for at least 6 months when stored at +5 \u00b0C.<\/jats:p>","DOI":"10.3390\/pharmaceutics15061602","type":"journal-article","created":{"date-parts":[[2023,5,27]],"date-time":"2023-05-27T16:10:53Z","timestamp":1685203853000},"page":"1602","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Eudragit\u00ae FS Microparticles Containing Bacteriophages, Prepared by Spray-Drying for Oral Administration"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0633-1436","authenticated-orcid":false,"given":"Emilie","family":"Tabare","sequence":"first","affiliation":[{"name":"Laboratory of Pharmaceutics and Biopharmaceutics, Faculty of Pharmacy, Universit\u00e9 Libre de Bruxelles, 1050 Brussel, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiffany","family":"Dauchot","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmaceutics and Biopharmaceutics, Faculty of Pharmacy, Universit\u00e9 Libre de Bruxelles, 1050 Brussel, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christel","family":"Cochez","sequence":"additional","affiliation":[{"name":"Laboratory for Molecular and Cellular Technology, Queen Astrid Military Hospital, 1120 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5814-3563","authenticated-orcid":false,"given":"Tea","family":"Glonti","sequence":"additional","affiliation":[{"name":"Laboratory for Molecular and Cellular Technology, Queen Astrid Military Hospital, 1120 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9line","family":"Antoine","sequence":"additional","affiliation":[{"name":"Food Science Department, FARAH and Faculty of Veterinary Medicine, University of Li\u00e8ge, 4000 Li\u00e8ge, Belgium"},{"name":"Bacteriology, Department of Infectious and Parasitic Diseases, FARAH and Faculty of Veterinary Medicine, University of Li\u00e8ge, 4000 Li\u00e8ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0088-4307","authenticated-orcid":false,"given":"Fanny","family":"Lafor\u00eat","sequence":"additional","affiliation":[{"name":"Food Science Department, FARAH and Faculty of Veterinary Medicine, University of Li\u00e8ge, 4000 Li\u00e8ge, Belgium"},{"name":"Bacteriology, Department of Infectious and Parasitic Diseases, FARAH and Faculty of Veterinary Medicine, University of Li\u00e8ge, 4000 Li\u00e8ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6863-5219","authenticated-orcid":false,"given":"Jean-Paul","family":"Pirnay","sequence":"additional","affiliation":[{"name":"Laboratory for Molecular and Cellular Technology, Queen Astrid Military Hospital, 1120 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V\u00e9ronique","family":"Delcenserie","sequence":"additional","affiliation":[{"name":"Food Science Department, FARAH and Faculty of Veterinary Medicine, University of Li\u00e8ge, 4000 Li\u00e8ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5161-7364","authenticated-orcid":false,"given":"Damien","family":"Thiry","sequence":"additional","affiliation":[{"name":"Bacteriology, Department of Infectious and Parasitic Diseases, FARAH and Faculty of Veterinary Medicine, University of Li\u00e8ge, 4000 Li\u00e8ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan","family":"Goole","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmaceutics and Biopharmaceutics, Faculty of Pharmacy, Universit\u00e9 Libre de Bruxelles, 1050 Brussel, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"S122","DOI":"10.1038\/nm1145","article-title":"Antibacterial Resistance Worldwide: Causes, Challenges and Responses","volume":"10","author":"Levy","year":"2004","journal-title":"Nat. Med."},{"key":"ref_2","first-page":"8","article-title":"La phagoth\u00e9rapie: Une arme cr\u00e9dible face \u00e0 l\u2019antibior\u00e9sistance","volume":"33","author":"Dufour","year":"2017","journal-title":"Med. Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1080\/1040841X.2020.1729695","article-title":"Phage Therapy Efficacy: A Review of the Last 10 Years of Preclinical Studies","volume":"46","author":"Melo","year":"2020","journal-title":"Crit. Rev. Microbiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"e01923-17","DOI":"10.1128\/mBio.01923-17","article-title":"Are Phage Lytic Proteins the Secret Weapon to Kill Staphylococcus aureus?","volume":"9","year":"2018","journal-title":"mBio"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"e00012-19","DOI":"10.1128\/MMBR.00012-19","article-title":"Pharmacologically Aware Phage Therapy: Pharmacodynamic and Pharmacokinetic Obstacles to Phage Antibacterial Action in Animal and Human Bodies","volume":"83","author":"Abedon","year":"2019","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1038\/s41575-021-00536-z","article-title":"Bacteriophages and Their Potential for Treatment of Gastrointestinal Diseases","volume":"19","author":"Duan","year":"2021","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Zalewska-Pi\u0105tek, B., and Pi\u0105tek, R. (2020). Phage Therapy as a Novel Strategy in the Treatment of Urinary Tract Infections Caused by E. Coli. Antibiotics, 9.","DOI":"10.3390\/antibiotics9060304"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Newman, J.W., Floyd, R.V., and Fothergill, J.L. (2017). The Contribution of Pseudomonas Aeruginosa Virulence Factors and Host Factors in the Establishment of Urinary Tract Infections. FEMS Microbiol. Lett., 364.","DOI":"10.1093\/femsle\/fnx124"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1155\/2005\/385768","article-title":"Complicated Urinary Tract Infection in Adults","volume":"16","author":"Nicolle","year":"2005","journal-title":"Can. J. Infect. Dis. Med. Microbiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"736","DOI":"10.1136\/gutjnl-2013-304786","article-title":"Shanghai Fever: A Distinct Pseudomonas aeruginosa Enteric Disease","volume":"63","author":"Chuang","year":"2014","journal-title":"Gut"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"121206","DOI":"10.1016\/j.ijpharm.2021.121206","article-title":"Enteric-Coated Bacteriophage Tablets for Oral Administration against Gastrointestinal Infections","volume":"609","author":"Khanal","year":"2021","journal-title":"Int. J. Pharm."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"4511","DOI":"10.1128\/IAI.00428-10","article-title":"Translocation of Pseudomonas aeruginosa from the Intestinal Tract Is Mediated by the Binding of ExoS to an Na,K-ATPase Regulator, FXYD3","volume":"78","author":"Okuda","year":"2010","journal-title":"Infect. Immun."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1263","DOI":"10.1007\/s001340100979","article-title":"Endemicity, Molecular Diversity and Colonisation Routes of Pseudomonas Aeruginosa in Intensive Care Units","volume":"27","author":"Bertrand","year":"2001","journal-title":"Intensive Care Med."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1097\/00000658-199308000-00001","article-title":"The Gastrointestinal Tract The \u201cUndrained Abscess\u201d of Multiple Organ Failure","volume":"218","author":"Marshall","year":"1993","journal-title":"Ann. Surg."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1007\/BF00259435","article-title":"The Effect of Selective Decontamination of the Digestive Tract on Colonisation and Infection Rate in Multiple Trauma Patients","volume":"10","author":"Stoutenbeek","year":"1984","journal-title":"Intensive Care Med."},{"key":"ref_16","first-page":"303","article-title":"Selective Decontamination of the Digestive Tract: A Stratified, Randomized, Prospective Study in a Mixed Intensive Care Unit","volume":"110","author":"Blair","year":"1991","journal-title":"Surgery"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1016\/S0140-6736(88)91656-X","article-title":"Triple Regimen of Selective Decontamination of the Digestive Tract, Systemic Cefotaxime, and Microbiological Surveillance for Prevention of Acquired Infection in Intensive Care","volume":"1","author":"Ledingham","year":"1988","journal-title":"Lancet"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1011","DOI":"10.1016\/S0140-6736(03)14409-1","article-title":"Effects of Selective Decontamination of Digestive Tract on Mortality and Acquisition of Resistant Bacteria in Intensive Care: A Randomised Controlled Trial","volume":"362","author":"Schultz","year":"2003","journal-title":"Lancet"},{"key":"ref_19","first-page":"467","article-title":"Means to Facilitate the Overcoming of Gastric Juice Barrier by a Therapeutic Staphylococcal Bacteriophage A5\/80","volume":"8","author":"Bubak","year":"2017","journal-title":"Front. Microbiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/j.cis.2017.05.014","article-title":"Formulation, Stabilisation and Encapsulation of Bacteriophage for Phage Therapy","volume":"249","author":"Malik","year":"2017","journal-title":"Adv. Colloid Interface Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"4799","DOI":"10.1128\/AEM.00246-08","article-title":"Microencapsulation of Bacteriophage Felix O1 into Chitosan-Alginate Microspheres for Oral Delivery","volume":"74","author":"Ma","year":"2008","journal-title":"AEM"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1016\/j.foodhyd.2010.11.017","article-title":"Enhanced Alginate Microspheres as Means of Oral Delivery of Bacteriophage for Reducing Staphylococcus Aureus Intestinal Carriage","volume":"26","author":"Ma","year":"2012","journal-title":"Food Hydrocoll."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1016\/j.lwt.2018.09.043","article-title":"Encapsulation of Bacteriophage T4 in Mannitol-Alginate Dry Macrospheres and Survival in Simulated Gastrointestinal Conditions","volume":"99","author":"Niezgoda","year":"2019","journal-title":"LWT"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.ejbt.2017.11.006","article-title":"Encapsulation of Specific Salmonella Enteritidis Phage F3\u03b1SE on Alginate-Spheres as a Method for Protection and Dosification","volume":"31","author":"Soto","year":"2018","journal-title":"Electron. J. Biotechnol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"913","DOI":"10.1111\/ijfs.12736","article-title":"Application of Chitosan-Alginate Microspheres for the Sustained Release of Bacteriophage in Simulated Gastrointestinal Conditions","volume":"50","author":"Kim","year":"2015","journal-title":"Int. J. Food Sci. Technol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1200","DOI":"10.1002\/mabi.201200109","article-title":"Novel Biopolymer Matrices for Microencapsulation of Phages: Enhanced Protection Against Acidity and Protease Activity: Novel Biopolymer Matrices for Microencapsulation of Phages: Enhanced Protection","volume":"12","author":"Dini","year":"2012","journal-title":"Macromol. Biosci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"460","DOI":"10.1016\/j.foodres.2012.12.037","article-title":"Whey Protein Improves Survival and Release Characteristics of Bacteriophage Felix O1 Encapsulated in Alginate Microspheres","volume":"52","author":"Tang","year":"2013","journal-title":"Food Res. Int."},{"key":"ref_28","first-page":"334","article-title":"Carrier Systems for Bacteriophages to Supplement Food Systems: Encapsulation and Controlled Release to Modulate the Human Gut Microbiota","volume":"7","author":"Samtlebe","year":"2016","journal-title":"Food Sci. Technol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1111\/j.1365-2672.2006.02918.x","article-title":"Bovine Whey Proteins Inhibit the Interaction of Staphylococcus Aureus and Bacteriophage K","volume":"101","author":"Gill","year":"2006","journal-title":"J. Appl. Microbiol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.ejpb.2008.12.001","article-title":"A Freeze-Dried Formulation of Bacteriophage Encapsulated in Biodegradable Microspheres","volume":"72","author":"Puapermpoonsiri","year":"2009","journal-title":"Eur. J. Pharm. Biopharm."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Gonz\u00e1lez-Men\u00e9ndez, E., Fern\u00e1ndez, L., Guti\u00e9rrez, D., Pando, D., Mart\u00ednez, B., Rodr\u00edguez, A., and Garc\u00eda, P. (2018). Strategies to Encapsulate the Staphylococcus Aureus Bacteriophage PhiIPLA-RODI. Viruses, 10.","DOI":"10.3390\/v10090495"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Vinner, G.K., Vladisavljevi\u0107, G.T., Clokie, M.R.J., and Malik, D.J. (2017). Microencapsulation of Clostridium Difficile Specific Bacteriophages Using Microfluidic Glass Capillary Devices for Colon Delivery Using PH Triggered Release. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0186239"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/j.ijpharm.2018.04.063","article-title":"Microfluidic-Assisted Bacteriophage Encapsulation into Liposomes","volume":"545","author":"Leung","year":"2018","journal-title":"Int. J. Pharm."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1016\/j.resmic.2018.05.011","article-title":"High Precision Microfluidic Microencapsulation of Bacteriophages for Enteric Delivery","volume":"169","author":"Vinner","year":"2018","journal-title":"Res. Microbiol."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Vinner, G.K., Rezaie-Yazdi, Z., Leppanen, M., Stapley, A.G.F., Leaper, M.C., and Malik, D.J. (2019). Microencapsulation of Salmonella-Specific Bacteriophage Felix O1 Using Spray-Drying in a Ph-Responsive Formulation and Direct Compression Tableting of Powders into a Solid Oral Dosage Form. Pharmaceuticals, 12.","DOI":"10.3390\/ph12010043"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1486","DOI":"10.1007\/s11095-016-1892-6","article-title":"Production of Inhalation Phage Powders Using Spray Freeze Drying and Spray Drying Techniques for Treatment of Respiratory Infections","volume":"33","author":"Leung","year":"2016","journal-title":"Pharm. Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.ijpharm.2017.01.060","article-title":"Effects of Storage Conditions on the Stability of Spray Dried, Inhalable Bacteriophage Powders","volume":"521","author":"Leung","year":"2017","journal-title":"Int. J. Pharm."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"578","DOI":"10.1016\/j.ejpb.2012.12.022","article-title":"Feasibility of Spray Drying Bacteriophages into Respirable Powders to Combat Pulmonary Bacterial Infections","volume":"84","author":"Vandenheuvel","year":"2013","journal-title":"Eur. J. Pharm. Biopharm."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1016\/j.ijpharm.2014.06.026","article-title":"Instability of Bacteriophages in Spray-Dried Trehalose Powders Is Caused by Crystallization of the Matrix","volume":"4","author":"Vandenheuvel","year":"2014","journal-title":"Int. J. Pharm."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Tabare, E., Glonti, T., Cochez, C., Ngassam, C., Pirnay, J.-P., Amighi, K., and Goole, J. (2021). A Design of Experiment Approach to Optimize Spray-Dried Powders Containing Pseudomonas AeruginosaPodoviridae and Myoviridae Bacteriophages. Viruses, 13.","DOI":"10.3390\/v13101926"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1304","DOI":"10.4315\/0362-028X-73.7.1304","article-title":"Oral Delivery Systems for Encapsulated Bacteriophages Targeted at Escherichia Coli O157:H7 in Feedlot Cattle","volume":"73","author":"Stanford","year":"2010","journal-title":"J. Food Prot."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"13893","DOI":"10.1038\/s41598-019-50461-0","article-title":"Development of a QPCR Platform for Quantification of the Five Bacteriophages within Bacteriophage Cocktail 2 (BFC2)","volume":"9","author":"Duyvejonck","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Antoine, C., Lafor\u00eat, F., Blasdel, B., Fall, A., Duprez, J.-N., Mainil, J., Delcenserie, V., and Thiry, D. (2021). In Vitro Characterization and In Vivo Efficacy Assessment in Galleria Mellonella Larvae of Newly Isolated Bacteriophages against Escherichia Coli K1. Viruses, 13.","DOI":"10.3390\/v13102005"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1007\/978-1-60327-164-6_14","article-title":"Phage Host Range and Efficiency of Plating","volume":"Volume 501","author":"Clokie","year":"2009","journal-title":"Bacteriophages"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1016\/j.ejpb.2011.09.014","article-title":"Design Space Approach in the Optimization of the Spray-Drying Process","volume":"80","author":"Lebrun","year":"2012","journal-title":"Eur. J. Pharm. Biopharm."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Tietje, C., and Brouder, A. (2010). Handbook of Transnational Economic Governance Regimes, Brill|Nijhoff.","DOI":"10.1163\/ej.9789004163300.i-1081"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ejpb.2017.09.002","article-title":"Production of Highly Stable Spray Dried Phage Formulations for Treatment of Pseudomonas Aeruginosa Lung Infection","volume":"121","author":"Chang","year":"2017","journal-title":"Eur. J. Pharm. Biopharm."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Lechanteur, A., and Evrard, B. (2020). Influence of Composition and Spray-Drying Process Parameters on Carrier-Free DPI Properties and Behaviors in the Lung: A Review. Pharmaceutics, 12.","DOI":"10.3390\/pharmaceutics12010055"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.ejpb.2019.09.014","article-title":"The Use of Hydrophobic Amino Acids in Protecting Spray Dried Trehalose Formulations against Moisture-Induced Changes","volume":"144","author":"Mah","year":"2019","journal-title":"Eur. J. Pharm. Biopharm."},{"key":"ref_50","unstructured":"Ali Rashidinejad, S.M.J. (2021). Spray Drying Encapsulation of Bioactive Materials, CRC Press."},{"key":"ref_51","unstructured":"Malik, D.J. (2019). Microbiome and Metabolome in Diagnosis, Therapy, and other Strategic Applications, Elsevier."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"388","DOI":"10.3109\/02652048.2011.651503","article-title":"Spray-Dried Microparticles: A Potential Vehicle for Oral Delivery of Vaccines","volume":"29","author":"Chablani","year":"2012","journal-title":"J. Microencapsul."},{"key":"ref_53","first-page":"61","article-title":"In Vitro In Vivo Comparison of Two PH Sensitive Eudragit Polymers for Colon Specific Drug Delivery","volume":"10","author":"Kshirsagar","year":"2009","journal-title":"J. Pharm. Sci."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"20523","DOI":"10.1039\/C7CP04124A","article-title":"The Amorphous State: First-Principles Derivation of the Gordon\u2013Taylor Equation for Direct Prediction of the Glass Transition Temperature of Mixtures; Estimation of the Crossover Temperature of Fragile Glass Formers; Physical Basis of the \u201cRule of 2\/3\u201d","volume":"19","author":"Skrdla","year":"2017","journal-title":"Phys. Chem. Chem. Phys."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"e10159","DOI":"10.1002\/btm2.10159","article-title":"Inhalable Bacteriophage Powders: Glass Transition Temperature and Bioactivity Stabilization","volume":"5","author":"Chang","year":"2020","journal-title":"Bioeng. Transl. Med."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Pirnay, J.-P., Verbeken, G., Ceyssens, P.-J., Huys, I., De Vos, D., Ameloot, C., and Fauconnier, A. (2018). The Magistral Phage. Viruses, 10.","DOI":"10.3390\/v10020064"}],"container-title":["Pharmaceutics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4923\/15\/6\/1602\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:43:27Z","timestamp":1760125407000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4923\/15\/6\/1602"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,5,27]]},"references-count":56,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2023,6]]}},"alternative-id":["pharmaceutics15061602"],"URL":"https:\/\/doi.org\/10.3390\/pharmaceutics15061602","relation":{},"ISSN":["1999-4923"],"issn-type":[{"value":"1999-4923","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,5,27]]}}}