{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T17:11:13Z","timestamp":1790356273074,"version":"4.1.0"},"reference-count":49,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2023,6,14]],"date-time":"2023-06-14T00:00:00Z","timestamp":1686700800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceutics"],"abstract":"<jats:p>Bioactive materials were developed with the ability to release fluoride and provide some antimicrobial potential, to be widely used in dentistry today. However, few scientific studies have evaluated the antimicrobial activity of bioactive surface pre-reacted glass (S-PRG) coatings (PRG Barrier Coat, Shofu, Kyoto, Japan) on periodontopathogenic biofilms. This study evaluated the antibacterial activity of S-PRG fillers on the microbial profile of multispecies subgingival biofilms. A Calgary Biofilm Device (CBD) was used to grow a 33-species biofilm related to periodontitis for 7 days. The S-PRG coating was applied on CBD pins from the test group and photo-activated (PRG Barrier Coat, Shofu), while the control group received no coating. Seven days after treatment, the total bacterial counts, metabolic activity, and microbial profile of the biofilms were observed using a colorimetric assay and DNA\u2013DNA hybridization. Statistical analyses were applied; namely, the Mann\u2013Whitney, Kruskal\u2013Wallis, and Dunn\u2019s post hoc tests. The bacterial activity of the test group was reduced by 25.7% compared with that of the control group. A statistically significant reduction was observed for the counts of 15 species: A. naeslundii, A. odontolyticus, V. parvula, C. ochracea, C. sputigena, E. corrodens, C. gracilis, F. nucleatum polymorphum, F. nucleatum vincentii, F. periodonticum, P. intermedia, P. gingivalis, G. morbillorum, S. anginosus, and S. noxia (p \u2264 0.05). The bioactive coating containing S-PRG modified the composition of the subgingival biofilm in vitro, thereby decreasing colonization by pathogens.<\/jats:p>","DOI":"10.3390\/pharmaceutics15061727","type":"journal-article","created":{"date-parts":[[2023,6,15]],"date-time":"2023-06-15T01:32:57Z","timestamp":1686792777000},"page":"1727","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Antibacterial Activity of a Bioactive Tooth-Coating Material Containing Surface Pre-Reacted Glass in a Complex Multispecies Subgingival Biofilm"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5127-3020","authenticated-orcid":false,"given":"Caio Junji","family":"Tanaka","sequence":"first","affiliation":[{"name":"School of Dentistry, Mogi das Cruzes University, Mogi das Cruzes 08780-911, Sao Paulo, Brazil"},{"name":"Dental Research Division, Department of Periodontology and Restorative Dentistry, Guarulhos University UNIVERITAS\/UNG, Guarulhos 07011-010, Sao Paulo, Brazil"},{"name":"School of Dentistry, Sao Judas Tadeu University, Sao Paulo 05503-001, Sao Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4172-1499","authenticated-orcid":false,"given":"Jos\u00e9 Augusto","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Dental Research Division, Department of Periodontology and Restorative Dentistry, Guarulhos University UNIVERITAS\/UNG, Guarulhos 07011-010, Sao Paulo, Brazil"},{"name":"School of Dentistry, Sao Judas Tadeu University, Sao Paulo 05503-001, Sao Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o Marcos Spessoto","family":"Pingueiro","sequence":"additional","affiliation":[{"name":"Dental Research Division, Department of Periodontology and Restorative Dentistry, Guarulhos University UNIVERITAS\/UNG, Guarulhos 07011-010, Sao Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatiane Tiemi","family":"Macedo","sequence":"additional","affiliation":[{"name":"Dental Research Division, Department of Periodontology and Restorative Dentistry, Guarulhos University UNIVERITAS\/UNG, Guarulhos 07011-010, Sao Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2293-3392","authenticated-orcid":false,"given":"Magda","family":"Feres","sequence":"additional","affiliation":[{"name":"Dental Research Division, Department of Periodontology and Restorative Dentistry, Guarulhos University UNIVERITAS\/UNG, Guarulhos 07011-010, Sao Paulo, Brazil"},{"name":"Department of Oral Medicine, Infection, and Immunity, Division of Periodontology, Harvard School of Dental Medicine, Boston, MA 02115, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1971-0195","authenticated-orcid":false,"given":"Jamil Awad","family":"Shibli","sequence":"additional","affiliation":[{"name":"Dental Research Division, Department of Periodontology and Restorative Dentistry, Guarulhos University UNIVERITAS\/UNG, Guarulhos 07011-010, Sao Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3275-5910","authenticated-orcid":false,"given":"Bruno","family":"Bueno-Silva","sequence":"additional","affiliation":[{"name":"Dental Research Division, Department of Periodontology and Restorative Dentistry, Guarulhos University UNIVERITAS\/UNG, Guarulhos 07011-010, Sao Paulo, Brazil"},{"name":"Department of Biosciences, Piracicaba Dental School, University of Campinas\u2014UNICAMP, Piracicaba 13414-903, Sao Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,6,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e026","DOI":"10.1590\/1807-3107bor-2020.vol34.0026","article-title":"Periodontal disease and its impact on general health in Latin America. 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