{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T18:49:41Z","timestamp":1790707781348,"version":"4.1.0"},"reference-count":132,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2023,7,28]],"date-time":"2023-07-28T00:00:00Z","timestamp":1690502400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Erasmus Mundus fellowship from the European Commission","award":["BT16PD3454"],"award-info":[{"award-number":["BT16PD3454"]}]},{"name":"Inserm","award":["BT16PD3454"],"award-info":[{"award-number":["BT16PD3454"]}]},{"name":"Rouen University","award":["BT16PD3454"],"award-info":[{"award-number":["BT16PD3454"]}]},{"name":"Normandy region","award":["BT16PD3454"],"award-info":[{"award-number":["BT16PD3454"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceutics"],"abstract":"<jats:p>Research on the neuroprotective effect of pituitary adenylate cyclase-activating polypeptide (PACAP) and its use as a therapeutic agent has grown over the past 30 years. Both in vitro and in vivo experiments have shown that PACAP exerts a strong neuroprotective effect in many central and peripheral neuronal diseases. Various delivery routes have been employed from intravenous (IV) injections to intracerebroventricular (ICV) administration, leading either to systemic or topical delivery of the peptide. Over the last decade, a growing interest in the use of intranasal (IN) administration of PACAP and other therapeutic agents has emerged as an alternative delivery route to target the brain. The aim of this review is to summarize the findings on the neuroprotective effect of PACAP and to discuss how the IN administration of PACAP could contribute to target the effects of this pleiotropic peptide.<\/jats:p>","DOI":"10.3390\/pharmaceutics15082032","type":"journal-article","created":{"date-parts":[[2023,7,28]],"date-time":"2023-07-28T07:35:24Z","timestamp":1690529724000},"page":"2032","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["The Potential of the Nose-to-Brain Delivery of PACAP for the Treatment of Neuronal Disease"],"prefix":"10.3390","volume":"15","author":[{"given":"Asma","family":"Cherait","sequence":"first","affiliation":[{"name":"Univ Rouen Normandie, Inserm U1245, Medical Faculty, Normandie Univ, F-76000 Rouen, France"},{"name":"Department of Second Cycle, Higher School of Agronomy Mostaganem, Mostaganem 27000, Algeria"},{"name":"Laboratory of Cellular Toxicology, Department of Biology, Faculty of Sciences, University of Badji Mokhtar Annaba, B.P. 12, Annaba 23000, Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William A.","family":"Banks","sequence":"additional","affiliation":[{"name":"Geriatric Research Educational and Clinical Center, Veterans Affairs Puget Sound Health Care System, Seattle, WA 98108, USA"},{"name":"Division of Gerontology and Geriatric Medicine, Department of Medicine, University of Washington School of Medicine, Seattle, WA 98104, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3567-7452","authenticated-orcid":false,"given":"David","family":"Vaudry","sequence":"additional","affiliation":[{"name":"Univ Rouen Normandie, Inserm U1245, Medical Faculty, Normandie Univ, F-76000 Rouen, France"},{"name":"Univ Rouen Normandie, Inserm US51, Regional Cell Imaging Platform of Normandy (PRIMACEN), Sciences and Technologies Faculty, Normandie Univ, F-76000 Rouen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,7,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1016\/0006-291X(89)91757-9","article-title":"Isolation of a novel 38 residue-hypothalamic polypeptide which stimulates adenylate cyclase in pituitary cells","volume":"164","author":"Miyata","year":"1989","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1124\/pr.109.001370","article-title":"Pituitary adenylate cyclase-activating polypeptide and its receptors: 20 years after the discovery","volume":"61","author":"Vaudry","year":"2009","journal-title":"Pharmacol. Rev."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1515\/cmble-2015-0008","article-title":"Therapeutic potential of PACAP for neurodegenerative diseases","volume":"20","author":"Yang","year":"2015","journal-title":"Cell. Mol. Biol. Lett."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"780","DOI":"10.1208\/s12248-015-9719-7","article-title":"Intranasal Delivery of Proteins and Peptides in the Treatment of Neurodegenerative Diseases","volume":"17","author":"Meredith","year":"2015","journal-title":"AAPS J."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2787","DOI":"10.1210\/endo-129-5-2787","article-title":"Tissue Distribution of PACAP as Determined by RIA: Highly Abundant in the Rat Brain and Testes","volume":"129","author":"Arimura","year":"1991","journal-title":"Endocrinology"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1111\/j.1476-5381.2012.01871.x","article-title":"Pharmacology and functions of receptors for vasoactive intestinal peptide and pituitary adenylate cyclase-activating polypeptide: IUPHAR review 1","volume":"166","author":"Harmar","year":"2012","journal-title":"Br. J. Pharmacol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"3892","DOI":"10.2174\/1381612824666181112110934","article-title":"Alternative Routes of Administration of the Neuroprotective Pituitary Adenylate Cyclase Activating Polypeptide","volume":"24","author":"Reglodi","year":"2018","journal-title":"Curr. Pharm. Des."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1002\/(SICI)1097-4547(19980915)53:6<651::AID-JNR3>3.0.CO;2-4","article-title":"Opposing mitogenic regulation by PACAP in sympathetic and cerebral cortical precursors correlates with differential expression of PACAP receptor (PAC1-R) isoforms","volume":"53","author":"Lu","year":"1998","journal-title":"J. Neurosci. Res."},{"key":"ref_9","first-page":"619","article-title":"The origin and function of the pituitary adenylate cyclase-activating polypeptide (PACAP)\/glucagon superfamily","volume":"21","author":"Sherwood","year":"2000","journal-title":"Endocr. Rev."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1031","DOI":"10.3389\/fnins.2019.01031","article-title":"Neuroprotective Potential of Pituitary Adenylate Cyclase Activating Polypeptide in Retinal Degenerations of Metabolic Origin","volume":"13","year":"2019","journal-title":"Front. Neurosci."},{"key":"ref_11","first-page":"269","article-title":"Pituitary adenylate cyclase-activating polypeptide and its receptors: From structure to functions","volume":"52","author":"Vaudry","year":"2000","journal-title":"Pharmacol. Rev."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2064","DOI":"10.1016\/j.neurobiolaging.2014.03.022","article-title":"Pituitary adenylate cyclase-activating polypeptide protects against \u03b2-amyloid toxicity","volume":"35","author":"Han","year":"2014","journal-title":"Neurobiol. Aging"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1007\/s12031-016-0731-x","article-title":"Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) Is Involved in Adult Mouse Hippocampal Neurogenesis After Stroke","volume":"59","author":"Matsumoto","year":"2016","journal-title":"J. Mol. Neurosci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"585082","DOI":"10.3389\/fendo.2020.585082","article-title":"Intranasal Administration of PACAP Is an Efficient Delivery Route to Reduce Infarct Volume and Promote Functional Recovery After Transient and Permanent Middle Cerebral Artery Occlusion","volume":"11","author":"Cherait","year":"2021","journal-title":"Front. Endocrinol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"204","DOI":"10.2174\/138161211795049679","article-title":"Protective effects of pituitary adenylate cyclase-activating polypeptide (PACAP) against apoptosis","volume":"17","author":"Seaborn","year":"2011","journal-title":"Curr. Pharm. Des."},{"key":"ref_16","unstructured":"Lamine-Ajili, A. (2018). Les Propri\u00e9t\u00e9s Antiapoptotiques et Antiautophagiques du Pituitary Adenylate Cyclase-Activating Polypeptide Assurent une Protection Neuronale dans des Mod\u00e8les In Vitro et In Vivo de la Maladie de Parkinson. [Ph.D. Thesis, University of Rouen Normandy]."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"797541","DOI":"10.3389\/fphar.2021.797541","article-title":"Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) Protects Striatal Cells and Improves Motor Function in Huntington\u2019s Disease Models: Role of PAC1 Receptor","volume":"12","author":"Lefranc","year":"2022","journal-title":"Front. Pharmacol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"e1917623117","DOI":"10.1073\/pnas.1917623117","article-title":"PACAP is a pathogen-inducible resident antimicrobial neuropeptide affording rapid and contextual molecular host defense of the brain","volume":"118","author":"Lee","year":"2021","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1001\/jamaneurol.2014.3625","article-title":"Association of pituitary adenylate cyclase-activating polypeptide with cognitive decline in mild cognitive impairment due to Alzheimer disease","volume":"72","author":"Han","year":"2015","journal-title":"JAMA Neurol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"3208","DOI":"10.1096\/fj.10-180133","article-title":"Neuropeptide pituitary adenylate cyclase-activating polypeptide (PACAP) slows down Alzheimer\u2019s disease-like pathology in amyloid precursor protein-transgenic mice","volume":"25","author":"Rat","year":"2011","journal-title":"FASEB J."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"131727","DOI":"10.1016\/j.snb.2022.131727","article-title":"Quantitative analysis of PACAP-38 and amyloid beta composition using a dual reaction in reduced graphene oxide biosensor to verify the neuroprotective role of PACAP-38","volume":"361","author":"Lee","year":"2022","journal-title":"Sens. Actuators B Chem."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1196\/annals.1317.072","article-title":"Comparative study of the effects of PACAP in young, aging, and castrated males in a rat model of Parkinson\u2019s disease","volume":"1070","author":"Toth","year":"2006","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Jungling, A., Reglodi, D., Maasz, G., Zrinyi, Z., Schmidt, J., Rivnyak, A., Horvath, G., Pirger, Z., and Tamas, A. (2021). Alterations of Nigral Dopamine Levels in Parkinson\u2019s Disease after Environmental Enrichment and PACAP Treatment in Aging Rats. Life, 11.","DOI":"10.3390\/life11010035"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1411","DOI":"10.1161\/01.STR.31.6.1411","article-title":"Delayed systemic administration of PACAP38 is neuroprotective in transient middle cerebral artery occlusion in the rat","volume":"31","author":"Reglodi","year":"2000","journal-title":"Stroke"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2227","DOI":"10.1016\/S0196-9781(02)00262-0","article-title":"Effects of pretreatment with PACAP on the infarct size and functional outcome in rat permanent focal cerebral ischemia","volume":"23","author":"Reglodi","year":"2002","journal-title":"Peptides"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1016\/j.freeradbiomed.2021.08.011","article-title":"Pituitary adenylate cyclase-activating polypeptide attenuates mitochondria-mediated oxidative stress and neuronal apoptosis after subarachnoid hemorrhage in rats","volume":"174","author":"Fang","year":"2021","journal-title":"Free Radic. Biol. Med."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1007\/s12031-008-9077-3","article-title":"Role of PACAP in ischemic neural death","volume":"36","author":"Ohtaki","year":"2008","journal-title":"J. Mol. Neurosci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1207","DOI":"10.1016\/j.peptides.2011.04.003","article-title":"PACAP and a novel stable analog protect rat brain from ischemia: Insight into the mechanisms of action","volume":"32","author":"Dejda","year":"2011","journal-title":"Peptides"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"520","DOI":"10.1161\/STROKEAHA.114.006864","article-title":"Delayed pituitary adenylate cyclase-activating polypeptide delivery after brain stroke improves functional recovery by inducing m2 microglia\/macrophage polarization","volume":"46","author":"Brifault","year":"2015","journal-title":"Stroke"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"4309","DOI":"10.1007\/s12035-021-02381-2","article-title":"Pituitary Adenylate Cyclase-Activating Polypeptide Protects Against Cognitive Impairment Caused by Chronic Cerebral Hypoperfusion","volume":"58","author":"Guo","year":"2021","journal-title":"Mol. Neurobiol."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Tan, Y.V., Abad, C., Wang, Y., Lopez, R., and Waschek, J.A. (2013). Pituitary adenylate cyclase activating peptide deficient mice exhibit impaired thymic and extrathymic regulatory T cell proliferation during EAE. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0061200"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1016\/j.peptides.2015.08.006","article-title":"PACAP as a neuroprotective factor in ischemic neuronal injuries","volume":"72","author":"Shioda","year":"2015","journal-title":"Peptides"},{"key":"ref_33","first-page":"127","article-title":"Pituitary adenylate cyclase-activating polypeptide (PACAP) has a neuroprotective function in dopamine-based neurodegeneration in rat and snail parkinsonian models","volume":"10","author":"Maasz","year":"2017","journal-title":"Dis. Models Mech."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1703","DOI":"10.2174\/0929867327666200320162436","article-title":"Implications of VIP and PACAP in Parkinson\u2019s Disease: What do we Know So Far?","volume":"28","author":"Ribeiro","year":"2021","journal-title":"Curr. Med. Chem."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"2368","DOI":"10.1210\/en.2002-0106","article-title":"Microarray and suppression subtractive hybridization analyses of gene expression in pheochromocytoma cells reveal pleiotropic effects of pituitary adenylate cyclase-activating polypeptide on cell proliferation, survival, and adhesion","volume":"144","author":"Grumolato","year":"2003","journal-title":"Endocrinology"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1007\/s12031-008-9075-5","article-title":"Peroxiredoxin 2 is involved in the neuroprotective effects of PACAP in cultured cerebellar granule neurons","volume":"36","author":"Botia","year":"2008","journal-title":"J. Mol. Neurosci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1007\/s12031-008-9132-0","article-title":"Influence of PACAP on oxidative stress and tissue injury following small-bowel autotransplantation","volume":"37","author":"Ferencz","year":"2009","journal-title":"J. Mol. Neurosci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1007\/s12031-010-9350-0","article-title":"Regulation of oxidative stress by pituitary adenylate cyclase-activating polypeptide (PACAP) mediated by PACAP receptor","volume":"42","author":"Ohtaki","year":"2010","journal-title":"J. Mol. Neurosci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"913","DOI":"10.1111\/jnc.13614","article-title":"Involvement of endogenous antioxidant systems in the protective activity of pituitary adenylate cyclase-activating polypeptide against hydrogen peroxide-induced oxidative damages in cultured rat astrocytes","volume":"137","author":"Douiri","year":"2016","journal-title":"J. Neurochem."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1016\/S0306-4522(98)00606-X","article-title":"Reduction of lipopolysaccharide-induced neurotoxicity in mixed cortical neuron\/glia cultures by femtomolar concentrations of pituitary adenylate cyclase-activating polypeptide","volume":"91","author":"Kong","year":"1999","journal-title":"Neuroscience"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1124\/jpet.106.102236","article-title":"Pituitary adenylate cyclase-activating polypeptide (PACAP) 38 and PACAP4-6 are neuroprotective through inhibition of NADPH oxidase: Potent regulators of microglia-mediated oxidative stress","volume":"319","author":"Yang","year":"2006","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/S0304-3940(96)12468-X","article-title":"Cyclic AMP and pituitary adenylate cyclase-activating polypeptide (PACAP) prevent programmed cell death of cultured rat cerebellar granule cells","volume":"206","author":"Chang","year":"1996","journal-title":"Neurosci. Lett."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/S0167-0115(02)00190-8","article-title":"Pituitary adenylate cyclase-activating polypeptide (PACAP) prevents hippocampal neurons from apoptosis by inhibiting JNK\/SAPK and p38 signal transduction pathways","volume":"109","author":"Dohi","year":"2002","journal-title":"Regul. Pept."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1231","DOI":"10.1111\/j.1471-4159.2004.02810.x","article-title":"Opposite regulation of the mitochondrial apoptotic pathway by C2-ceramide and PACAP through a MAP-kinase-dependent mechanism in cerebellar granule cells","volume":"91","author":"Aubert","year":"2004","journal-title":"J. Neurochem."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1666","DOI":"10.1111\/j.1471-4159.2007.04895.x","article-title":"Pituitary adenylate cyclase-activating polypeptide is up-regulated in cortical pyramidal cells after focal ischemia and protects neurons from mild hypoxic\/ischemic damage","volume":"103","author":"Stumm","year":"2007","journal-title":"J. Neurochem."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1007\/s12031-019-01309-9","article-title":"PACAP Attenuates Optic Nerve Crush-Induced Retinal Ganglion Cell Apoptosis Via Activation of the CREB-Bcl-2 Pathway","volume":"68","author":"Ye","year":"2019","journal-title":"J. Mol. Neurosci."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1237","DOI":"10.1111\/j.1471-4159.2006.04148.x","article-title":"PACAP and C2-ceramide generate different AP-1 complexes through a MAP-kinase-dependent pathway: Involvement of c-Fos in PACAP-induced Bcl-2 expression","volume":"99","author":"Aubert","year":"2006","journal-title":"J. Neurochem."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1371","DOI":"10.1016\/j.bbabio.2006.06.014","article-title":"Necrosis, a well-orchestrated form of cell demise: Signalling cascades, important mediators and concomitant immune response","volume":"1757","author":"Festjens","year":"2006","journal-title":"Biochim Biophys Acta"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1152\/physrev.00041.2016","article-title":"Origin and Consequences of Necroinflammation","volume":"98","author":"Sarhan","year":"2018","journal-title":"Physiol. Rev."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1954","DOI":"10.1007\/s13311-020-00925-3","article-title":"Pituitary Adenylate Cyclase-Activating Polypeptide Attenuates Brain Edema by Protecting Blood-Brain Barrier and Glymphatic System After Subarachnoid Hemorrhage in Rats","volume":"17","author":"Fang","year":"2020","journal-title":"Neurotherapeutics"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"688","DOI":"10.1016\/j.bbadis.2016.01.005","article-title":"Effect of the pituitary adenylate cyclase-activating polypeptide on the autophagic activation observed in in vitro and in vivo models of Parkinson\u2019s disease","volume":"1862","author":"Fahmy","year":"2016","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"D\u2019Amico, A.G., Maugeri, G., Saccone, S., Federico, C., Cavallaro, S., Reglodi, D., and D\u2019Agata, V. (2020). PACAP Modulates the Autophagy Process in an In Vitro Model of Amyotrophic Lateral Sclerosis. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21082943"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.mcn.2014.08.007","article-title":"PACAP induces plasticity at autonomic synapses by nAChR-dependent NOS1 activation and AKAP-mediated PKA targeting","volume":"63","author":"Jayakar","year":"2014","journal-title":"Mol. Cell. Neurosci."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Rivnyak, A., Kiss, P., Tamas, A., Balogh, D., and Reglodi, D. (2018). Review on PACAP-Induced Transcriptomic and Proteomic Changes in Neuronal Development and Repair. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19041020"},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Shibato, J., Takenoya, F., Yamashita, M., Gupta, R., Min, C.W., Kim, S.T., Kimura, A., Takasaki, I., Hori, M., and Shioda, S. (2023). OMICS Analyses Unraveling Related Gene and Protein-Driven Molecular Mechanisms Underlying PACAP 38-Induced Neurite Outgrowth in PC12 Cells. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24044169"},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Ogata, K., Shintani, N., Hayata-Takano, A., Kamo, T., Higashi, S., Seiriki, K., Momosaki, H., Vaudry, D., Vaudry, H., and Galas, L. (2015). PACAP enhances axon outgrowth in cultured hippocampal neurons to a comparable extent as BDNF. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0120526"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1007\/s12031-010-9372-7","article-title":"The involvement of PACAP\/VIP system in the synaptic transmission in the hippocampus","volume":"42","author":"Yang","year":"2010","journal-title":"J. Mol. Neurosci."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"4208","DOI":"10.1523\/JNEUROSCI.2468-18.2019","article-title":"Pituitary Adenylate Cyclase-Activating Polypeptide Modulates Dendritic Spine Maturation and Morphogenesis via MicroRNA-132 Upregulation","volume":"39","author":"Kamo","year":"2019","journal-title":"J. Neurosci."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"353","DOI":"10.3389\/fnmol.2018.00353","article-title":"Activation of Serotonin 5-HT7 Receptors Modulates Hippocampal Synaptic Plasticity by Stimulation of Adenylate Cyclases and Rescues Learning and Behavior in a Mouse Model of Fragile X Syndrome","volume":"11","author":"Costa","year":"2018","journal-title":"Front. Mol. Neurosci."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1002\/jnr.20038","article-title":"PACAP promotes neural stem cell proliferation in adult mouse brain","volume":"76","author":"Mercer","year":"2004","journal-title":"J. Neurosci. Res."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1038\/nrn1824","article-title":"Astrocyte-endothelial interactions at the blood-brain barrier","volume":"7","author":"Abbott","year":"2006","journal-title":"Nat. Rev. Neurosci."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1152\/physrev.00050.2017","article-title":"Blood-Brain barrier: From physiology to disease and back","volume":"99","author":"Sweeney","year":"2019","journal-title":"Physiol. Rev."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1038\/jcbfm.2008.131","article-title":"Isolation of peptide transport system-6 from brain endothelial cells: Therapeutic effects with antisense inhibition in Alzheimer and stroke models","volume":"29","author":"Kumar","year":"2009","journal-title":"J. Cereb. Blood Flow Metab."},{"key":"ref_64","first-page":"690","article-title":"Passage of pituitary adenylate cyclase activating polypeptide1-27 and pituitary adenylate cyclase activating polypeptide1-38 across the blood-brain barrier","volume":"267","author":"Banks","year":"1993","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1186\/s10194-018-0861-3","article-title":"Transport of the pituitary adenylate cyclase-activating polypeptide across the blood-brain barrier: Implications for migraine","volume":"19","author":"Amin","year":"2018","journal-title":"J. Headache Pain"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.npep.2016.03.005","article-title":"The pituitary adenylate cyclase-activating polypeptide (PACAP) protects adrenal function in septic rats administered etomidate","volume":"58","author":"Liu","year":"2016","journal-title":"Neuropeptides"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"2131","DOI":"10.1038\/npp.2014.61","article-title":"Intranasal Delivery of a Peptide with Antidepressant-Like Effect","volume":"39","author":"Brown","year":"2014","journal-title":"Neuropsychopharmacol"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"1349","DOI":"10.1016\/j.peptides.2005.11.010","article-title":"Pituitary adenylate cyclase-activating peptide (PACAP): Assessment of dipeptidyl peptidase IV degradation, insulin-releasing activity and antidiabetic potential","volume":"27","author":"Green","year":"2006","journal-title":"Peptides"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1016\/j.peptides.2008.01.022","article-title":"Novel stable PACAP analogs with potent activity towards the PAC1 receptor","volume":"29","author":"Bourgault","year":"2008","journal-title":"Peptides"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"1846","DOI":"10.1038\/npp.2015.34","article-title":"Pituitary adenylate cyclase-activating peptide in the central amygdala causes anorexia and body weight loss via the melanocortin and the trkb systems","volume":"40","author":"Iemolo","year":"2015","journal-title":"Neuropsychopharmacology"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"2563","DOI":"10.2353\/ajpath.2010.090941","article-title":"Pituitary adenylate cyclase activating polypeptide: An important vascular regulator in human skin in vivo","volume":"177","author":"Seeliger","year":"2010","journal-title":"Am. J. Pathol."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/j.regpep.2006.12.010","article-title":"The effect of intravenous PACAP38 on cerebral hemodynamics in healthy volunteers","volume":"140","author":"Birk","year":"2007","journal-title":"Regul. Pept."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/S0167-0115(98)00037-8","article-title":"PACAP and PACAP receptors in insulin producing tissues: Localization and effects","volume":"74","author":"Filipsson","year":"1998","journal-title":"Regul. Pept."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/j.brainresbull.2018.10.009","article-title":"Evaluation of intranasal delivery route of drug administration for brain targeting","volume":"143","author":"Bors","year":"2018","journal-title":"Brain Res. Bull."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"534","DOI":"10.1208\/s12249-011-9614-1","article-title":"Effects of localized hydrophilic mannitol and hydrophobic nelfinavir administration targeted to olfactory epithelium on brain distribution","volume":"12","author":"Hoekman","year":"2011","journal-title":"AAPS PharmSciTech"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1016\/j.expneurol.2013.01.015","article-title":"Intranasal delivery of cell-penetrating anti-NF-\u03baB peptides (Tat-NBD) alleviates infection-sensitized hypoxic-ischemic brain injury","volume":"247","author":"Yang","year":"2013","journal-title":"Exp. Neurol."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"1654","DOI":"10.1002\/jps.21924","article-title":"Intranasal delivery to the central nervous system: Mechanisms and experimental considerations","volume":"99","author":"Dhuria","year":"2010","journal-title":"J. Pharm. Sci."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"1068495","DOI":"10.3389\/fphar.2022.1068495","article-title":"Systemic and brain delivery of antidiabetic peptides through nasal administration using cell-penetrating peptides","volume":"13","author":"Maeng","year":"2022","journal-title":"Front. Pharmacol."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"3754","DOI":"10.3390\/molecules14093754","article-title":"Nasal delivery of high molecular weight drugs","volume":"14","author":"Ozsoy","year":"2009","journal-title":"Molecules"},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.jconrel.2018.05.011","article-title":"Nose-to-brain drug delivery: An update on clinical challenges and progress towards approval of anti-Alzheimer drugs","volume":"281","author":"Agrawal","year":"2018","journal-title":"J. Control. Release"},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"1","DOI":"10.2217\/14796708.3.1.1","article-title":"The nose may help the brain: Intranasal drug delivery for treating neuro-logical diseases","volume":"3","author":"Ying","year":"2008","journal-title":"Future Neurol."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1016\/j.peptides.2012.05.021","article-title":"Intranasal administration of PACAP: Uptake by brain and regional brain targeting with cyclodextrins","volume":"36","author":"Nonaka","year":"2012","journal-title":"Peptides"},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1046\/j.1471-4159.2000.740501.x","article-title":"Synergistic induction of pituitary adenylate cyclase-activating polypeptide (PACAP) gene expression by nerve growth factor and PACAP in PC12 cells","volume":"74","author":"Hashimoto","year":"2000","journal-title":"J. Neurochem."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"8263","DOI":"10.1007\/s12035-018-0972-5","article-title":"Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) Enhances Hippocampal Synaptic Plasticity and Improves Memory Performance in Huntington\u2019s Disease","volume":"55","author":"Masana","year":"2018","journal-title":"Mol. Neurobiol."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"370ra181","DOI":"10.1126\/scitranslmed.aaf9526","article-title":"Adenylyl cyclase activating polypeptide reduces phosphorylation and toxicity of the polyglutamine-expanded androgen receptor in spinobulbar muscular atrophy","volume":"8","author":"Polanco","year":"2016","journal-title":"Sci. Transl. Med."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"1361","DOI":"10.3233\/JAD-190707","article-title":"Molecular Mechanisms of Intranasal Insulin in SAMP8 Mice","volume":"71","author":"Rhea","year":"2019","journal-title":"J. Alzheimers Dis."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1007\/s13346-021-00940-7","article-title":"Intranasal drug delivery for treatment of Alzheimer\u2019s disease","volume":"11","author":"Fonseca","year":"2021","journal-title":"Drug Deliv. Transl. Res."},{"key":"ref_88","first-page":"240","article-title":"Intranasal Insulin Reduces White Matter Hyperintensity Progression in Association with Improvements in Cognition and CSF Biomarker Profiles in Mild Cognitive Impairment and Alzheimer\u2019s Disease","volume":"8","author":"Kellar","year":"2021","journal-title":"J. Prev. Alzheimers Dis."},{"key":"ref_89","unstructured":"(2021, December 10). ClinicalTrials.gov Identifier (NCT Number): NCT05006599. Study of Nasal Insulin to Fight Forgetfulness (SNIFF)-3-Week Aptar CPS Device, Available online: https:\/\/clinicaltrials.gov\/ct2\/show\/NCT05006599."},{"key":"ref_90","unstructured":"(2021, December 10). I-Mab Announce about First Patient Dosed in U.S. Phase 1 Study of Protollin for the Treatment of Alzheimer\u2019s Disease. Available online: https:\/\/ir.i-mabbiopharma.com\/news-releases\/news-release-details\/i-mab-announces-first-patient-dosed-us-phase-1-study-protollin."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1186\/s13287-022-03234-y","article-title":"Safety and efficacy outcomes after intranasal administration of neural stem cells in cerebral palsy: A randomized phase 1\/2 controlled trial","volume":"14","author":"Lv","year":"2023","journal-title":"Stem Cell Res. Ther."},{"key":"ref_92","first-page":"439","article-title":"Non-Invasive Strategies for Nose-to-Brain Drug Delivery","volume":"10","author":"Trevino","year":"2020","journal-title":"J. Clin. Trials"},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"288","DOI":"10.18433\/J3NC79","article-title":"Intranasal drug delivery: How, why and what for?","volume":"12","author":"Pires","year":"2009","journal-title":"J. Pharm. Pharm. Sci."},{"key":"ref_94","doi-asserted-by":"crossref","unstructured":"Ghadiri, M., Young, P.M., and Traini, D. (2019). Strategies to Enhance Drug Absorption via Nasal and Pulmonary Routes. Pharmaceutics, 11.","DOI":"10.3390\/pharmaceutics11030113"},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1111\/epi.16449","article-title":"Bioavailability and safety of diazepam intranasal solution compared to oral and rectal diazepam in healthy volunteers","volume":"61","author":"Hogan","year":"2020","journal-title":"Epilepsia"},{"key":"ref_96","first-page":"1","article-title":"Imaging of intranasal drug delivery to the brain","volume":"10","author":"Veronesi","year":"2020","journal-title":"Am. J. Nucl. Med. Mol. Imaging"},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1111\/j.1365-2362.2007.01832.x","article-title":"Pulmonary and systemic effects of inhaled PACAP38 in healthy male subjects","volume":"37","author":"Doberer","year":"2007","journal-title":"Eur. J. Clin. Investig."},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1002\/cne.20934","article-title":"Localization and characterization of pituitary adenylate cyclase-activating polypeptide receptors in the human cerebellum during development","volume":"496","author":"Basille","year":"2006","journal-title":"J. Comp. Neurol."},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1002\/cne.21451","article-title":"Molecular, cellular, and functional characterizations of pituitary adenylate cyclase-activating polypeptide and its receptors in the cerebellum of New and Old World monkeys","volume":"504","author":"Aubert","year":"2007","journal-title":"J. Comp. Neurol."},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.nbd.2008.06.014","article-title":"PACAP prevents toxicity induced by cisplatin in rat and primate neurons but not in proliferating ovary cells: Involvement of the mitochondrial apoptotic pathway","volume":"32","author":"Aubert","year":"2008","journal-title":"Neurobiol. Dis."},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1016\/j.neuroscience.2009.02.028","article-title":"Distribution and functional characterization of pituitary adenylate cyclase-activating polypeptide receptors in the brain of non-human primates","volume":"160","author":"Jolivel","year":"2009","journal-title":"Neuroscience"},{"key":"ref_102","first-page":"1381","article-title":"Pep-lipid cubosomes and vesicles compartmentalized by micelles from self-assembly of multiple neuroprotective building blocks including a large peptide hormone PACAP-DHA","volume":"5","author":"Angelova","year":"2019","journal-title":"Chem. Nano Mat."},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"1016","DOI":"10.1016\/j.ymthe.2020.02.009","article-title":"Pre-arrayed Pan-AAV Peptide Display Libraries for Rapid Single-Round Screening","volume":"28","author":"Kienle","year":"2020","journal-title":"Mol. Ther."},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"747726","DOI":"10.3389\/fnins.2021.747726","article-title":"A Multifaceted Approach to Optimizing AAV Delivery to the Brain for the Treatment of Neurodegenerative Diseases","volume":"15","author":"Fischell","year":"2021","journal-title":"Front. Neurosci."},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"862","DOI":"10.1007\/s13346-021-01086-2","article-title":"Using nanotechnology to deliver biomolecules from nose to brain\u2014Peptides, proteins, monoclonal antibodies and RNA","volume":"12","author":"Borrajo","year":"2022","journal-title":"Drug Deliv. Transl. Res."},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.trsl.2022.05.002","article-title":"Intranasal application of adeno-associated viruses: A systematic review","volume":"248","author":"Gadenstaetter","year":"2022","journal-title":"Transl. Res."},{"key":"ref_107","doi-asserted-by":"crossref","first-page":"3308","DOI":"10.1021\/jm900291j","article-title":"Molecular and conformational determinants of pituitary adenylate cyclase-activating polypeptide (PACAP) for activation of the PAC1 receptor","volume":"52","author":"Bourgault","year":"2009","journal-title":"J. Med. Chem."},{"key":"ref_108","doi-asserted-by":"crossref","first-page":"440","DOI":"10.1016\/j.neuropharm.2015.05.014","article-title":"Characterizations of a synthetic pituitary adenylate cyclase-activating polypeptide analog displaying potent neuroprotective activity and reduced in vivo cardiovascular side effects in a Parkinson\u2019s disease model","volume":"108","author":"Lamine","year":"2016","journal-title":"Neuropharmacology"},{"key":"ref_109","doi-asserted-by":"crossref","first-page":"818003","DOI":"10.3389\/fddsv.2021.818003","article-title":"Design and Synthesis of Brain Penetrant Glycopeptide Analogues of PACAP With Neuroprotective Potential for Traumatic Brain Injury and Parkinsonism","volume":"1","author":"Apostol","year":"2022","journal-title":"Front. Drug Discov."},{"key":"ref_110","first-page":"634","article-title":"Nasal Absorption of Drugs\u2013Barriers and Solutions","volume":"2","author":"Dehghan","year":"2009","journal-title":"Res. J. Pharm. Technol."},{"key":"ref_111","doi-asserted-by":"crossref","first-page":"183","DOI":"10.4155\/tde-2019-0086","article-title":"Nasal formulations for drug administration and characterization of nasal preparations in drug delivery","volume":"11","year":"2020","journal-title":"Ther. Deliv."},{"key":"ref_112","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1007\/s13346-020-00891-5","article-title":"Intranasal drug delivery: Opportunities and toxicologic challenges during drug development","volume":"12","author":"Keller","year":"2022","journal-title":"Drug Deliv. Transl. Res."},{"key":"ref_113","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.brainresbull.2020.04.012","article-title":"Modulation of nose-to-brain delivery of a P-glycoprotein (MDR1) substrate model drug (quinidine) in rats","volume":"160","author":"Bors","year":"2020","journal-title":"Brain Res. Bull."},{"key":"ref_114","doi-asserted-by":"crossref","first-page":"055101","DOI":"10.1088\/0957-4484\/25\/5\/055101","article-title":"Enhanced blood-brain barrier transmigration using a novel transferrin embedded fluorescent magneto-liposome nanoformulation","volume":"25","author":"Ding","year":"2014","journal-title":"Nanotechnology"},{"key":"ref_115","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1615\/CritRevTherDrugCarrierSyst.2018024697","article-title":"Updated Progress of Nanocarrier-Based Intranasal Drug Delivery Systems for Treatment of Brain Diseases","volume":"35","author":"Fan","year":"2018","journal-title":"Crit. Rev. Ther. Drug Carr. Syst."},{"key":"ref_116","doi-asserted-by":"crossref","unstructured":"Sonvico, F., Clementino, A., Buttini, F., Colombo, G., Pescina, S., Stanis\u00e7uaski Guterres, S., Raffin Pohlmann, A., and Nicoli, S. (2018). Surface-Modified Nanocarriers for Nose-to-Brain Delivery: From Bioadhesion to Targeting. Pharmaceutics, 10.","DOI":"10.20944\/preprints201802.0077.v1"},{"key":"ref_117","doi-asserted-by":"crossref","first-page":"9183","DOI":"10.1038\/s41598-019-45137-8","article-title":"gH625-liposomes as tool for pituitary adenylate cyclase-activating polypeptide brain delivery","volume":"9","author":"Iachetta","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_118","doi-asserted-by":"crossref","unstructured":"Montegiove, N., Calzoni, E., Emiliani, C., and Cesaretti, A. (2022). Biopolymer Nanoparticles for Nose-to-Brain Drug Delivery: A New Promising Approach for the Treatment of Neurological Diseases. J. Funct. Biomater., 13.","DOI":"10.3390\/jfb13030125"},{"key":"ref_119","doi-asserted-by":"crossref","unstructured":"Marcello, E., and Chiono, V. (2023). Biomaterials-Enhanced Intranasal Delivery of Drugs as a Direct Route for Brain Targeting. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24043390"},{"key":"ref_120","doi-asserted-by":"crossref","first-page":"822","DOI":"10.1007\/s13346-022-01245-z","article-title":"Transferrin-modified chitosan nanoparticles for targeted nose-to-brain delivery of proteins","volume":"13","author":"Gabold","year":"2023","journal-title":"Drug Deliv. Transl. Res."},{"key":"ref_121","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1016\/j.jconrel.2007.05.026","article-title":"Brain delivery of vasoactive intestinal peptide enhanced with the nanoparticles conjugated with wheat germ agglutinin following intranasal administration","volume":"121","author":"Gao","year":"2007","journal-title":"J. Control. Release"},{"key":"ref_122","doi-asserted-by":"crossref","first-page":"4198","DOI":"10.2174\/1381612823666170727144450","article-title":"Biotin and Glutathione Targeting of Solid Nanoparticles to Cross Human Brain Endothelial Cells","volume":"23","author":"Veszelka","year":"2017","journal-title":"Curr. Pharm. Des."},{"key":"ref_123","doi-asserted-by":"crossref","first-page":"3870","DOI":"10.1016\/j.biomaterials.2013.02.003","article-title":"Lactoferrin-modified PEG-co-PCL nanoparticles for enhanced brain delivery of NAP peptide following intranasal administration","volume":"34","author":"Liu","year":"2013","journal-title":"Biomaterials"},{"key":"ref_124","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/j.neulet.2008.10.021","article-title":"Transduction of PACAP38 protects primary cortical neurons from neurotoxic injury","volume":"448","author":"Sanchez","year":"2008","journal-title":"Neurosci. Lett."},{"key":"ref_125","doi-asserted-by":"crossref","first-page":"6","DOI":"10.3389\/fnsyn.2020.00006","article-title":"Monosynaptic Tracing Success Depends Critically on Helper Virus Concentrations","volume":"12","author":"Lavin","year":"2020","journal-title":"Front. Synaptic Neurosci."},{"key":"ref_126","doi-asserted-by":"crossref","first-page":"14051","DOI":"10.1038\/mtm.2014.51","article-title":"Widespread gene transfer in the central nervous system of cynomolgus macaques following delivery of AAV9 into the cisterna magna. Molecular therapy","volume":"1","author":"Hinderer","year":"2014","journal-title":"Methods Clin. Dev."},{"key":"ref_127","doi-asserted-by":"crossref","first-page":"988914","DOI":"10.3389\/fnmol.2022.988914","article-title":"Routes of administration for adeno-associated viruses carrying gene therapies for brain diseases","volume":"15","author":"Zhou","year":"2022","journal-title":"Front. Mol. Neurosci."},{"key":"ref_128","doi-asserted-by":"crossref","first-page":"2242","DOI":"10.1016\/j.neuron.2022.05.003","article-title":"Engineered AAVs for non-invasive gene delivery to rodent and non-human primate nervous systems","volume":"110","author":"Chen","year":"2022","journal-title":"Neuron"},{"key":"ref_129","doi-asserted-by":"crossref","first-page":"93","DOI":"10.3389\/fnana.2019.00093","article-title":"Adeno-Associated Virus Technologies and Methods for Targeted Neuronal Manipulation","volume":"13","author":"Haery","year":"2019","journal-title":"Front. Neuroanat."},{"key":"ref_130","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/S0169-409X(97)00063-X","article-title":"Effects of physicochemical properties and other factors on systemic nasal drug delivery","volume":"29","author":"Romeo","year":"1998","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_131","doi-asserted-by":"crossref","unstructured":"Trenkel, M., and Scherlie\u00df, R. (2021). Nasal Powder Formulations: In-Vitro Characterisation of the Impact of Powders on Nasal Residence Time and Sensory Effects. Pharmaceutics, 13.","DOI":"10.3390\/pharmaceutics13030385"},{"key":"ref_132","doi-asserted-by":"crossref","first-page":"2300","DOI":"10.1016\/j.drudis.2022.04.012","article-title":"Engineered nasal dry powder for the encapsulation of bioactive compounds","volume":"27","author":"Baldelli","year":"2022","journal-title":"Drug Discov. Today"}],"container-title":["Pharmaceutics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4923\/15\/8\/2032\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:21:15Z","timestamp":1760127675000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4923\/15\/8\/2032"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,7,28]]},"references-count":132,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2023,8]]}},"alternative-id":["pharmaceutics15082032"],"URL":"https:\/\/doi.org\/10.3390\/pharmaceutics15082032","relation":{},"ISSN":["1999-4923"],"issn-type":[{"value":"1999-4923","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,7,28]]}}}