{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T07:40:19Z","timestamp":1771054819404,"version":"3.50.1"},"reference-count":39,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2023,8,3]],"date-time":"2023-08-03T00:00:00Z","timestamp":1691020800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceutics"],"abstract":"<jats:p>Acne is a chronic inflammatory skin disease that affects the quality of life of patients. Several treatments exist for acne, but their effectiveness tends to decrease over time due to increasing resistance to treatment and associated side effects. To circumvent these issues, a new approach has emerged that involves combating the pathogen Cutibacterium acnes while maintaining the homeostasis of the skin microbiome. Recently, it was shown that the use of a G2 lysine dendrigraft (G2 dendrimer) could specifically decrease the C. acnes phylotype (IAI) involved in acne, compared to non-acne-causing C. acnes (phylotype II) bacteria. In the present study, we demonstrate that the efficacy of this technology is related to its 3D structure, which, in contrast to the linear form, significantly decreases the inflammation factor (IL-8) linked to acne. In addition, our in-vitro data confirm the specific activity of the G2 dendrimer: after treatment of bacterial cultures and biofilms, the G2 dendrimer affected neither non-acneic C. acnes nor commensal bacteria of the skin (Staphylococcus epidermidis, S. hominis, and Corynebacterium minutissimum). In parallel, comparative in-vitro and in-vivo studies with traditional over-the-counter molecules showed G2\u2019s effects on the survival of commensal bacteria and the reduction of acne outbreaks. Finally, metagenomic analysis of the cutaneous microbiota of volunteers who applied a finished cosmetic product containing the G2 dendrimer confirmed the ability of G2 to rebalance cutaneous acne microbiota dysbiosis while maintaining commensal bacteria. These results confirm the value of using this G2 dendrimer to gently prevent the appearance of acne vulgaris while respecting the cutaneous microbiota.<\/jats:p>","DOI":"10.3390\/pharmaceutics15082083","type":"journal-article","created":{"date-parts":[[2023,8,3]],"date-time":"2023-08-03T11:13:06Z","timestamp":1691061186000},"page":"2083","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Lysine-Dendrimer, a New Non-Aggressive Solution to Rebalance the Microbiota of Acne-Prone Skin"],"prefix":"10.3390","volume":"15","author":[{"given":"Julie","family":"Leignadier","sequence":"first","affiliation":[{"name":"Lucas Meyer Cosmetics, 195 Route d\u2019Espagne, 31036 Toulouse, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marie","family":"Drago","sequence":"additional","affiliation":[{"name":"Shiseido EMEA, 56A Rue du Faubourg St Honor\u00e9, 75008 Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8235-8919","authenticated-orcid":false,"given":"Olivier","family":"Lesouhaitier","sequence":"additional","affiliation":[{"name":"Research Unit Bacterial Communication and Anti-Infectious Strategies (CBSA, UR4312), University of Rouen Normandie, 27000 Evreux, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Magalie","family":"Barreau","sequence":"additional","affiliation":[{"name":"Research Unit Bacterial Communication and Anti-Infectious Strategies (CBSA, UR4312), University of Rouen Normandie, 27000 Evreux, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Albert","family":"Dashi","sequence":"additional","affiliation":[{"name":"Sequential Skin Ltd., 85 Great Portland Street, London W1W 7LT, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oliver","family":"Worsley","sequence":"additional","affiliation":[{"name":"Sequential Skin Ltd., 85 Great Portland Street, London W1W 7LT, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5728-0926","authenticated-orcid":false,"given":"Joan","family":"Attia-Vigneau","sequence":"additional","affiliation":[{"name":"Lucas Meyer Cosmetics, 195 Route d\u2019Espagne, 31036 Toulouse, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1205","DOI":"10.1111\/ijd.16220","article-title":"What is new in adult acne for the last 2 years: Focus on acne pathophysiology and treatments","volume":"61","author":"Dagnelie","year":"2022","journal-title":"Int. J. Dermatol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"473","DOI":"10.2165\/00128071-200304070-00004","article-title":"Topical acne drugs: Review of clinical properties, systemic exposure, and safety","volume":"4","author":"Akhavan","year":"2003","journal-title":"Am. J. Clin. Dermatol."},{"key":"ref_3","first-page":"2123","article-title":"Diagnosis and treatment of acne","volume":"69","author":"Feldman","year":"2004","journal-title":"Am. Fam. Physician"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1111\/bjd.18316","article-title":"Cutibacterium acnes in acne pathophysiology\u2014The chicken or the egg?","volume":"181","author":"Ochsendorf","year":"2019","journal-title":"Br. J. Dermatol."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Brzuszkiewicz, E., Weiner, J., Wollherr, A., Th\u00fcrmer, A., H\u00fcpeden, J., Lomholt, H.B., Kilian, M., Gottschalk, G., Daniel, R., and Mollenkopf, H.-J. (2011). Comparative genomics and transcriptomics of Propionibacterium acnes. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0021581"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2152","DOI":"10.1038\/jid.2013.21","article-title":"Propionibacterium acnes strain populations in the human skin microbiome associated with acne","volume":"133","author":"Tomida","year":"2013","journal-title":"J. Investig. Dermatol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1111\/bjd.18230","article-title":"The role of the skin microbiota in acne pathophysiology","volume":"181","author":"Ramasamy","year":"2019","journal-title":"Br. J. Dermatol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1007\/s40257-020-00531-1","article-title":"The Skin Microbiome: A New Actor in Inflammatory Acne","volume":"21","author":"Dagnelie","year":"2020","journal-title":"Am. J. Clin. Dermatol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1016\/S0140-6736(11)60321-8","article-title":"Acne vulgaris","volume":"379","author":"Williams","year":"2012","journal-title":"Lancet"},{"key":"ref_10","first-page":"CD011154","article-title":"Topical benzoyl peroxide for acne","volume":"3","author":"Yang","year":"2020","journal-title":"Cochrane Database Syst. Rev."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"792","DOI":"10.1016\/j.jaad.2008.06.040","article-title":"An aqueous gel fixed combination of clindamycin phosphate 1.2% and benzoyl peroxide 2.5% for the once-daily treatment of moderate to severe acne vulgaris: Assessment of efficacy and safety in 2813 patients","volume":"59","author":"Thiboutot","year":"2008","journal-title":"J. Am. Acad. Dermatol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1590\/abd1806-4841.20175273","article-title":"Efficacy and safety of superficial chemical peeling in treatment of active acne vulgaris","volume":"92","author":"Hameed","year":"2017","journal-title":"An. Bras. Dermatol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1177\/12034754211037994","article-title":"The Impact of Acne Treatment on Skin Bacterial Microbiota: A Systematic Review","volume":"26","author":"Lam","year":"2022","journal-title":"J. Cutan. Med. Surg."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1186\/s40168-018-0558-5","article-title":"Host-microbiome interactions and recent progress into understanding the biology of acne vulgaris","volume":"6","author":"Gallo","year":"2018","journal-title":"Microbiome"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1001\/jamadermatol.2018.5221","article-title":"Association of Systemic Antibiotic Treatment of Acne with Skin Microbiota Characteristics","volume":"155","author":"Chien","year":"2019","journal-title":"JAMA Dermatol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1038\/nrmicro2537","article-title":"The skin microbiome","volume":"9","author":"Grice","year":"2011","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"21104","DOI":"10.1038\/s41598-022-25436-3","article-title":"Skin dysbiosis and Cutibacterium acnes biofilm in inflammatory acne lesions of adolescents","volume":"12","author":"Cavallo","year":"2022","journal-title":"Sci. Rep."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1056","DOI":"10.1111\/exd.14554","article-title":"Polylysine dendrigraft is able to differentially impact Cutibacterium acnes strains preventing acneic skin","volume":"31","author":"Barreau","year":"2022","journal-title":"Exp. Dermatol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1038\/nbt1309","article-title":"Improved antimicrobial peptides based on acyl-lysine oligomers","volume":"25","author":"Radzishevsky","year":"2007","journal-title":"Nat. Biotechnol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"155","DOI":"10.3389\/fmed.2019.00155","article-title":"Acne and Stress: Impact of Catecholamines on Cutibacterium acnes","volume":"6","author":"Borrel","year":"2019","journal-title":"Front. Med."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"954","DOI":"10.1126\/science.1260144","article-title":"Dialogue between skin microbiota and immunity","volume":"346","author":"Belkaid","year":"2014","journal-title":"Science"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1001\/jamadermatol.2017.0904","article-title":"The Follicular Skin Microbiome in Patients with Hidradenitis Suppurativa and Healthy Controls","volume":"153","author":"Ring","year":"2017","journal-title":"JAMA Dermatol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2309","DOI":"10.1002\/chem.200901734","article-title":"An expeditious multigram-scale synthesis of lysine dendrigraft (DGL) polymers by aqueous N-carboxyanhydride polycondensation","volume":"16","author":"Collet","year":"2010","journal-title":"Chemistry"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2395","DOI":"10.1099\/00221287-146-10-2395","article-title":"Quantification of biofilm structures by the novel computer program COMSTAT","volume":"146","author":"Heydorn","year":"2000","journal-title":"Microbiology"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1038\/nrmicro.2017.157","article-title":"The human skin microbiome","volume":"16","author":"Byrd","year":"2018","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"953","DOI":"10.1002\/mbo3.138","article-title":"Comparative study of normal and sensitive skin aerobic bacterial populations","volume":"2","author":"Hillion","year":"2013","journal-title":"MicrobiologyOpen"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"e01032-20","DOI":"10.1128\/MRA.01032-20","article-title":"Draft Genome Sequences of Four Commensal Strains of Staphylococcus and Pseudomonas Isolated from Healthy Human Skin","volume":"10","author":"Janvier","year":"2021","journal-title":"Microbiol. Resour. Announc."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1038\/ismej.2008.44","article-title":"The tragedy of the uncommon: Understanding limitations in the analysis of microbial diversity","volume":"2","author":"Bent","year":"2008","journal-title":"ISME J."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"222","DOI":"10.2307\/3543712","article-title":"A Comparison of Three Diversity Indices Based on Their Components of Richness and Evenness","volume":"26","author":"DeJong","year":"1975","journal-title":"Oikos"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"e83","DOI":"10.1016\/j.ddmec.2012.12.003","article-title":"What lives on our skin: Ecology, genomics and therapeutic opportunities of the skin microbiome","volume":"10","author":"Scharschmidt","year":"2013","journal-title":"Drug Discov. Today Dis. Mech."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1514","DOI":"10.1101\/gr.191320.115","article-title":"The intersection of microbiome and host at the skin interface: Genomic- and metagenomic-based insights","volume":"25","author":"Grice","year":"2015","journal-title":"Genome Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1190","DOI":"10.1126\/science.1171700","article-title":"Topographical and Temporal Diversity of the Human Skin Microbiome","volume":"324","author":"Grice","year":"2009","journal-title":"Science"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"39491","DOI":"10.1038\/srep39491","article-title":"The balance of metagenomic elements shapes the skin microbiome in acne and health","volume":"6","author":"Barnard","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1745","DOI":"10.1099\/00221287-139-8-1745","article-title":"Interaction of Propionibacterium acnes with skin lipids in vitro","volume":"139","author":"Gribbon","year":"1993","journal-title":"J. Gen. Microbiol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"798","DOI":"10.1111\/exd.13296","article-title":"Skin microbiome and acne vulgaris: Staphylococcus, a new actor in acne","volume":"26","author":"Dreno","year":"2017","journal-title":"Exp. Dermatol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"3355","DOI":"10.1128\/JCM.00799-08","article-title":"Acne is Not Associated with Yet-Uncultured Bacteria","volume":"46","author":"Lomholt","year":"2008","journal-title":"J. Clin. Microbiol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1586\/edm.10.6","article-title":"Staphylococcus colonization of the skin and antimicrobial peptides","volume":"5","author":"Otto","year":"2010","journal-title":"Expert Rev. Dermatol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1449","DOI":"10.7164\/antibiotics.37.1449","article-title":"Antimicrobial action of epsilon-poly-L-lysine","volume":"37","author":"Shima","year":"1984","journal-title":"J. Antibiot."},{"key":"ref_39","first-page":"s73","article-title":"The role of benzoyl peroxide in the new treatment paradigm for acne","volume":"12","author":"Kircik","year":"2013","journal-title":"J. Drugs Dermatol."}],"container-title":["Pharmaceutics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4923\/15\/8\/2083\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:25:22Z","timestamp":1760127922000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4923\/15\/8\/2083"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,8,3]]},"references-count":39,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2023,8]]}},"alternative-id":["pharmaceutics15082083"],"URL":"https:\/\/doi.org\/10.3390\/pharmaceutics15082083","relation":{},"ISSN":["1999-4923"],"issn-type":[{"value":"1999-4923","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,8,3]]}}}