{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T13:28:35Z","timestamp":1771680515836,"version":"3.50.1"},"reference-count":45,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2024,4,19]],"date-time":"2024-04-19T00:00:00Z","timestamp":1713484800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"research projects RTI2018\u2013100903\u2013B\u2013I00 and PID2021\u2013125660OB\u2013100","award":["MCIN\/AEI\/10.13039\/501100011033\/FEDER"],"award-info":[{"award-number":["MCIN\/AEI\/10.13039\/501100011033\/FEDER"]}]},{"name":"research projects RTI2018\u2013100903\u2013B\u2013I00 and PID2021\u2013125660OB\u2013100","award":["FPU18\/01559"],"award-info":[{"award-number":["FPU18\/01559"]}]},{"name":"research projects RTI2018\u2013100903\u2013B\u2013I00 and PID2021\u2013125660OB\u2013100","award":["FPU19\/04169"],"award-info":[{"award-number":["FPU19\/04169"]}]},{"DOI":"10.13039\/501100020636","name":"Spanish Ministry of Education, Culture and Sport","doi-asserted-by":"publisher","award":["MCIN\/AEI\/10.13039\/501100011033\/FEDER"],"award-info":[{"award-number":["MCIN\/AEI\/10.13039\/501100011033\/FEDER"]}],"id":[{"id":"10.13039\/501100020636","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100020636","name":"Spanish Ministry of Education, Culture and Sport","doi-asserted-by":"publisher","award":["FPU18\/01559"],"award-info":[{"award-number":["FPU18\/01559"]}],"id":[{"id":"10.13039\/501100020636","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100020636","name":"Spanish Ministry of Education, Culture and Sport","doi-asserted-by":"publisher","award":["FPU19\/04169"],"award-info":[{"award-number":["FPU19\/04169"]}],"id":[{"id":"10.13039\/501100020636","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceutics"],"abstract":"<jats:p>The ABCG2 transporter plays a key role in pharmacological and toxicological processes, affecting bioavailability, tissue accumulation and milk secretion of its substrates. This protein is expressed in several biological barriers acting as a protective mechanism against xenobiotic exposure by pumping out a broad range of compounds. However, its induced expression during lactation in alveolar cells of mammary gland represents a relevant route for active transport of unwanted chemicals into milk. This work aimed to characterize the involvement of ABCG2 in systemic exposure and milk secretion of the flukicide nitroxynil. Using MDCK\u2013II cells overexpressing the transporter, we showed that nitroxynil is an in vitro substrate of different species variants of ABCG2. Moreover, using wild-type and Abcg2\u2212\/\u2212 mice, we showed that murine Abcg2 clearly affects plasma levels of nitroxynil. We also reported differences in nitroxynil accumulation in several tissues, with almost 2-fold higher concentration in kidney, small intestine and testis of Abcg2\u2212\/\u2212 mice. Finally, we proved that nitroxynil secretion into milk was also affected by Abcg2, with a 1.9-fold higher milk concentration in wild-type compared with Abcg2\u2212\/\u2212 mice. We conclude that ABCG2 significantly impacts nitroxynil biodistribution by regulating its passage across biological barriers.<\/jats:p>","DOI":"10.3390\/pharmaceutics16040558","type":"journal-article","created":{"date-parts":[[2024,4,19]],"date-time":"2024-04-19T10:53:17Z","timestamp":1713523997000},"page":"558","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["ABCG2 Transports the Flukicide Nitroxynil and Affects Its Biodistribution and Secretion into Milk"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0313-4034","authenticated-orcid":false,"given":"Laura","family":"\u00c1lvarez-Fern\u00e1ndez","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences-Physiology, Faculty of Veterinary Medicine, Animal Health Institute (INDEGSAL), University of Le\u00f3n, Campus de Vegazana s\/n, 24071 Le\u00f3n, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8334-1094","authenticated-orcid":false,"given":"Esther","family":"Blanco-Paniagua","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences-Physiology, Faculty of Veterinary Medicine, Animal Health Institute (INDEGSAL), University of Le\u00f3n, Campus de Vegazana s\/n, 24071 Le\u00f3n, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7620-3475","authenticated-orcid":false,"given":"Gracia","family":"Merino","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences-Physiology, Faculty of Veterinary Medicine, Animal Health Institute (INDEGSAL), University of Le\u00f3n, Campus de Vegazana s\/n, 24071 Le\u00f3n, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,4,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1333","DOI":"10.1124\/dmd.107.014902","article-title":"Expression of thirty-six drug transporter genes in human intestine, liver, kidney, and organotypic cell lines","volume":"35","author":"Hilgendorf","year":"2007","journal-title":"Drug Metab. 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