{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:00:20Z","timestamp":1760144420598,"version":"build-2065373602"},"reference-count":46,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2024,4,20]],"date-time":"2024-04-20T00:00:00Z","timestamp":1713571200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordination for the Improvement of Higher Education Personnel (CAPES)","doi-asserted-by":"publisher","award":["001","124847\/2021-8"],"award-info":[{"award-number":["001","124847\/2021-8"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"National Council for Scientific and Technological Development (CNPq)","doi-asserted-by":"publisher","award":["001","124847\/2021-8"],"award-info":[{"award-number":["001","124847\/2021-8"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceutics"],"abstract":"<jats:p>This work assessed the influence of the amount of dentifrice and fluoride (F) concentration in the product on the pH and inorganic components of Streptococcus mutans and Candida albicans dual-species biofilms. The biofilms were treated with suspensions of fluoride dentifrices containing 550 or 1100 ppm of F (550 F or 1100 F, respectively) administered at comparable intensities: (i-1) 550 F\/0.08 g or 1100 F\/0.04 g; (i-2) 550 F\/0.16 g or 1100 F\/0.08 g; and (i-3) 550 F\/0.32 g or 1100 F\/0.16 g. A placebo dentifrice (without NaF, 0.32 g) was used as a negative control. After the last treatment, the biofilm pH was measured and the F, calcium (Ca), and phosphorus (P) concentrations were determined. Data were subjected to an ANOVA\/Kruskal\u2013Wallis test, and a Student\u2013Newman\u2013Keuls test. The highest biofilm pH and F concentrations (biomass and fluid) were observed for 1100 F at i-3. Overall, 1100 F resulted in F levels similar to 550 F for i-1 and i-2. In addition, 550 F applied at i-2 and i-3 led to higher F in the biomass\/fluid compared to 1100 F applied at i-1 and i-2, respectively. In biomass, the lowest Ca concentrations were observed for 1100 F at i-3. The conclusion drawn is that the treatment intensity holds greater significance as a parameter compared to the concentration of F or the amount of dentifrice when considered individually.<\/jats:p>","DOI":"10.3390\/pharmaceutics16040562","type":"journal-article","created":{"date-parts":[[2024,4,22]],"date-time":"2024-04-22T09:53:52Z","timestamp":1713779632000},"page":"562","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Amount of Dentifrice and Fluoride Concentration Affect the pH and Inorganic Composition of Dual-Species Biofilms of Streptococcus mutans and Candida albicans"],"prefix":"10.3390","volume":"16","author":[{"given":"Caio","family":"Sampaio","sequence":"first","affiliation":[{"name":"Department of Preventive and Restorative Dentistry, School of Dentistry, S\u00e3o Paulo State University (UNESP), Ara\u00e7atuba 16015-050, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8159-4853","authenticated-orcid":false,"given":"Alberto Carlos Botazzo","family":"Delbem","sequence":"additional","affiliation":[{"name":"Department of Preventive and Restorative Dentistry, School of Dentistry, S\u00e3o Paulo State University (UNESP), Ara\u00e7atuba 16015-050, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thayse Yumi","family":"Hosida","sequence":"additional","affiliation":[{"name":"Department of Preventive and Restorative Dentistry, School of Dentistry, S\u00e3o Paulo State University (UNESP), Ara\u00e7atuba 16015-050, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Vit\u00f3ria Pereira","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Department of Preventive and Restorative Dentistry, School of Dentistry, S\u00e3o Paulo State University (UNESP), Ara\u00e7atuba 16015-050, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruna","family":"do Amaral","sequence":"additional","affiliation":[{"name":"Department of Preventive and Restorative Dentistry, School of Dentistry, S\u00e3o Paulo State University (UNESP), Ara\u00e7atuba 16015-050, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leonardo Ant\u00f4nio","family":"de Morais","sequence":"additional","affiliation":[{"name":"Department of Preventive and Restorative Dentistry, School of Dentistry, S\u00e3o Paulo State University (UNESP), Ara\u00e7atuba 16015-050, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Douglas Roberto","family":"Monteiro","sequence":"additional","affiliation":[{"name":"Department of Preventive and Restorative Dentistry, School of Dentistry, S\u00e3o Paulo State University (UNESP), Ara\u00e7atuba 16015-050, SP, Brazil"},{"name":"Graduate Program in Health Sciences, University of Western S\u00e3o Paulo (UNOESTE), Presidente Prudente 19050-920, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1550-3933","authenticated-orcid":false,"given":"Juliano Pelim","family":"Pessan","sequence":"additional","affiliation":[{"name":"Department of Preventive and Restorative Dentistry, School of Dentistry, S\u00e3o Paulo State University (UNESP), Ara\u00e7atuba 16015-050, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,4,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1318","DOI":"10.1126\/science.284.5418.1318","article-title":"Bacterial biofilms: A common cause of persistent infections","volume":"284","author":"Costerton","year":"1999","journal-title":"Science"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Flemming, H.C. 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