{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T20:32:55Z","timestamp":1790800375411,"version":"4.1.0"},"reference-count":52,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2025,1,13]],"date-time":"2025-01-13T00:00:00Z","timestamp":1736726400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","award":["2007\/02595-0"],"award-info":[{"award-number":["2007\/02595-0"]}]},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","award":["2007\/03135-3"],"award-info":[{"award-number":["2007\/03135-3"]}]},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","award":["2014\/22250-1"],"award-info":[{"award-number":["2014\/22250-1"]}]},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","award":["2021\/10265-8"],"award-info":[{"award-number":["2021\/10265-8"]}]},{"name":"Coordenadoria de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","award":["2007\/02595-0"],"award-info":[{"award-number":["2007\/02595-0"]}]},{"name":"Coordenadoria de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","award":["2007\/03135-3"],"award-info":[{"award-number":["2007\/03135-3"]}]},{"name":"Coordenadoria de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","award":["2014\/22250-1"],"award-info":[{"award-number":["2014\/22250-1"]}]},{"name":"Coordenadoria de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","award":["2021\/10265-8"],"award-info":[{"award-number":["2021\/10265-8"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceutics"],"abstract":"<jats:p>Background\/Objectives: Dithiocarbazates (DTCs) and their metal complexes have been studied regarding their property as anticancer activities. In this work, using S-benzyl-5-hydroxy-3-methyl-5-phenyl-4,5-dihydro-1H-pirazol-1-carbodithionate (H2bdtc), we prepared [ReO(bdtc)(Hbdtc)] and [[99mTc]TcO(bdtc)(Hbdtc)] complexes for tumor uptake and animal biodistribution studies. Methods: Re complex was prepared by a reaction of H2bdtc and (NBu4)[ReOCl4], the final product was characterized by IR, 1H NMR, CHN, and MS-ESI. 99mTc complex was prepared by the reaction of H2bdtc and [[99mTc]TcO4\u2212 and analyzed by planar and HPLC radiochromatography, and the stability was evaluated against amino acids and plasma. Biodistribution was performed in C57B\/6 mice with B16F10 and TM1M implanted tumor. Results: Re is asymmetric coordinated by two dithiocarbazate ligands, one with O,N,S chelation, and the other with N,S chelation; [[99mTc]TcO(bdtc)(Hbdtc)] was prepared with a radiochemical yield of around 93%. The radioactive complex is hydrophobic (LogP = 1.03), stable for 6 h in PBS and L-histidine solution; stable for 1 h in plasma, but unstable in the presence of L-cysteine. Ex vivo biodistribution demonstrated that the compound has a fast and persistent (until 2 h) uptake by the spleen (55.46%), and tumor B16F10 and TM1M uptake is lower than 1%. In vivo SPECT\/CT imaging confirmed ex vivo biodistribution, except by heterogenous TM1M accumulation but not in the B16-F10 lineage. Conclusions: H2bdtc proved to be an interesting chelator for rhenium or [99mTc]technetium. The right spleen uptake opened the opportunity to deepen the study of the molecule in this tissue and justifies future studies to identify the reason of heterogenous uptake in TM1M tumor uptake.<\/jats:p>","DOI":"10.3390\/pharmaceutics17010100","type":"journal-article","created":{"date-parts":[[2025,1,13]],"date-time":"2025-01-13T11:31:31Z","timestamp":1736767891000},"page":"100","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["[99mTc]Technetium and Rhenium Dithiocarbazate Complexes: Chemical Synthesis and Biological Assessment"],"prefix":"10.3390","volume":"17","author":[{"given":"Andr\u00e9 Gustavo","family":"de Araujo Fernandes","sequence":"first","affiliation":[{"name":"Instituto de Qu\u00edmica de S\u00e3o Carlos, Universidade de S\u00e3o Paulo, S\u00e3o Carlos 13566-590, SP, Brazil"},{"name":"Departamento de Ci\u00eancias Exatas, Universidade Estadual de Santa Cruz, Ilh\u00e9us 45662-900, BA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alyne Eloise","family":"Lafratta","sequence":"additional","affiliation":[{"name":"Laboratory of Nuclear Medicine (LIM-43), Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo 05403-911, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina Portela","family":"Luz","sequence":"additional","affiliation":[{"name":"Laboratory of Nuclear Medicine (LIM-43), Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo 05403-911, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7492-997X","authenticated-orcid":false,"given":"Debora","family":"Levy","sequence":"additional","affiliation":[{"name":"Lipids, Oxidation, and Cell Biology Team, Laboratory of Immunology (LIM19), Heart Institute (InCor), Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo 05403-900, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1766-2786","authenticated-orcid":false,"given":"Daniele","family":"de Paula Faria","sequence":"additional","affiliation":[{"name":"Laboratory of Nuclear Medicine (LIM-43), Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo 05403-911, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0956-2790","authenticated-orcid":false,"given":"Carlos Alberto","family":"Buchpiguel","sequence":"additional","affiliation":[{"name":"Laboratory of Nuclear Medicine (LIM-43), Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo 05403-911, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1747-7927","authenticated-orcid":false,"given":"Ulrich","family":"Abram","sequence":"additional","affiliation":[{"name":"Institute of Chemistry and Biochemistry, Freie Universit\u00e4t Berlin, Fabeckstr. 34-36, D-14195 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victor Marcelo","family":"Deflon","sequence":"additional","affiliation":[{"name":"Instituto de Qu\u00edmica de S\u00e3o Carlos, Universidade de S\u00e3o Paulo, S\u00e3o Carlos 13566-590, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7383-926X","authenticated-orcid":false,"given":"Fabio Luiz","family":"Navarro Marques","sequence":"additional","affiliation":[{"name":"Laboratory of Nuclear Medicine (LIM-43), Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo 05403-911, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,1,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1276","DOI":"10.1016\/j.jinorgbio.2010.08.009","article-title":"Dithiocarbazate complexes with the [M(PPh3)]2+ (M\u2550Pd or Pt) moiety: Synthesis, characterization and anti-Tripanosoma cruzi activity","volume":"104","author":"Silva","year":"2010","journal-title":"J. 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