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Recently, several research groups disclosed their large biological and chemical applications in different fields, leading to an increased interest towards this class of molecules. Some of the obtained derivatives have been successfully employed in the clinical treatment of different tumor types, but the onset of heavy side effects impaired their efficacy and discouraged their use. Pursuing the aim of obtaining carbazoles with less negative features, a lot of chemically modified compounds have been produced and evaluated. Objectives\/Methods: In this paper, we describe the in vitro and in vivo evaluation of a bis-carbazole derivative with strong anticancer properties against two breast cancer cell lines. Results: This compound has been found to impact the cell cytoskeleton dynamics, triggering the activation of some key proteins playing a role in the intrinsic and extrinsic apoptotic pathways. Equally important, this derivative has been found to be selective for cancer cells and has shown a safe profile in Balb\/c-treated mice. Conclusions: Overall, the disclosed outcomes represent an important landmark for encouraging further studies directed toward the potentiation of this lead to be potentially exploited in both preclinical and clinical applications.<\/jats:p>","DOI":"10.3390\/pharmaceutics17040415","type":"journal-article","created":{"date-parts":[[2025,3,25]],"date-time":"2025-03-25T08:42:35Z","timestamp":1742892155000},"page":"415","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Anti-Breast Cancer Properties and In Vivo Safety Profile of a Bis-Carbazole Derivative"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1386-3601","authenticated-orcid":false,"given":"Jessica","family":"Ceramella","sequence":"first","affiliation":[{"name":"Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, Via Pietro Bucci, 87036 Arcavacata di Rende, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2949-9215","authenticated-orcid":false,"given":"Camillo","family":"Rosano","sequence":"additional","affiliation":[{"name":"U.O. Proteomica e Spettrometria di Massa, IRCCS Ospedale Policlinico San Martino, Largo Rosanna Benzi, 10, 16132 Genova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5179-7118","authenticated-orcid":false,"given":"Domenico","family":"Iacopetta","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, Via Pietro Bucci, 87036 Arcavacata di Rende, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2194-8741","authenticated-orcid":false,"given":"Im\u00e9ne","family":"Ben Toumia","sequence":"additional","affiliation":[{"name":"U.O. Proteomica e Spettrometria di Massa, IRCCS Ospedale Policlinico San Martino, Largo Rosanna Benzi, 10, 16132 Genova, Italy"},{"name":"Laboratory of Bioactive Natural Substances and Biotechnology, Faculty of Dentistry of Monastir, University of Monastir, Monastir 5000, Tunisia"},{"name":"Laboratory of Molecular and Cellular Biology, Faculty of Dental Medicine of Monastir, University of Monastir, Monastir 5000, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leila","family":"Chekir-Ghedira","sequence":"additional","affiliation":[{"name":"Laboratory of Bioactive Natural Substances and Biotechnology, Faculty of Dentistry of Monastir, University of Monastir, Monastir 5000, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mouna","family":"Maatouk","sequence":"additional","affiliation":[{"name":"Laboratory of Bioactive Natural Substances and Biotechnology, Faculty of Dentistry of Monastir, University of Monastir, Monastir 5000, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9763-838X","authenticated-orcid":false,"given":"Annaluisa","family":"Mariconda","sequence":"additional","affiliation":[{"name":"Department of Basic and Applied Sciences, University of Basilicata, Via dell\u2019Ateneo Lucano, 10, 85100 Potenza, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7685-1093","authenticated-orcid":false,"given":"Pasquale","family":"Longo","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biology \u201cA. Zambelli\u201d, University of Salerno, Via Giovanni Paolo II, 132, 84084 Fisciano, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4845-615X","authenticated-orcid":false,"given":"Patrick","family":"Dallemagne","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Caen Normandie, Normandie University, CERMN UR4258, F-14000 Caen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7996-2082","authenticated-orcid":false,"given":"Christophe","family":"Rochais","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Caen Normandie, Normandie University, CERMN UR4258, F-14000 Caen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2435-8931","authenticated-orcid":false,"given":"Maria Stefania","family":"Sinicropi","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, Via Pietro Bucci, 87036 Arcavacata di Rende, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,25]]},"reference":[{"key":"ref_1","first-page":"881","article-title":"Anticancer carbazole alkaloids and coumarins from Clausena plants: A review","volume":"15","author":"Huang","year":"2017","journal-title":"Chin. 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