{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T13:29:20Z","timestamp":1791811760903,"version":"4.3.4"},"reference-count":86,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T00:00:00Z","timestamp":1745452800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Baghdad University and Deanship of Scientific Research, Vice Presidency for Graduate Studies and Scientific Research, King Faisal University","award":["KFU251401"],"award-info":[{"award-number":["KFU251401"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceutics"],"abstract":"<jats:p>Background\/Objective: Antimicrobial resistance (AMR) and therapy-resistant cancer cells represent major clinical challenges, necessitating the development of novel therapeutic strategies. This study explores the use of selenium nanoparticles (SeNPs) and colistin-conjugated selenium nanoparticles (Col-SeNPs) as a dual-function nanotherapeutic against multidrug-resistant Pseudomonas aeruginosa, antifungal-drug-resistant Candida spp., and human breast carcinoma (MCF-7) cells. Methods: SeNPs were synthesized and characterized using UV-Vis spectroscopy, atomic force microscopy (AFM), energy-dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), and Fourier-transform infrared spectroscopy (FTIR), confirming their nanoscale morphology, purity, and stability. Results: The antimicrobial activity of SeNPs and Col-SeNPs was assessed based on the minimum inhibitory concentration (MIC) and bacterial viability assays. Col-SeNPs exhibited enhanced antibacterial effects against P. aeruginosa, along with significant downregulation of the mexY efflux pump gene, which is associated with colistin resistance. Additionally, Col-SeNPs demonstrated superior antifungal activity against Candida albicans, C. glabrata, and C. krusei compared to SeNPs alone. The anticancer potential of Col-SeNPs was evaluated in MCF-7 cells using the MTT assay, revealing dose-dependent cytotoxicity through apoptosis and oxidative stress pathways. Although MCF-7 is not inherently drug-resistant, this model was used to explore the potential of Col-SeNPs in overcoming resistance mechanisms commonly encountered in cancer therapy. Conclusions: these findings support the promise of Col-SeNPs as a novel approach for addressing both antimicrobial resistance and cancer treatment challenges. Further in vivo studies, including pharmacokinetics and combination therapies, are warranted to advance clinical translation.<\/jats:p>","DOI":"10.3390\/pharmaceutics17050556","type":"journal-article","created":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T06:18:02Z","timestamp":1745475482000},"page":"556","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Colistin-Conjugated Selenium Nanoparticles: A Dual-Action Strategy Against Drug-Resistant Infections and Cancer"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4961-4532","authenticated-orcid":false,"given":"Mais E.","family":"Ahmed","sequence":"first","affiliation":[{"name":"Department of Biology, College of Science, University of Baghdad, Jadriya, Baghdad 10071, Iraq"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7560-6379","authenticated-orcid":false,"given":"Kholoud K.","family":"Alzahrani","sequence":"additional","affiliation":[{"name":"Department of Biology, University College of Umluj, University of Tabuk, Umluj 71491, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nedal M.","family":"Fahmy","sequence":"additional","affiliation":[{"name":"Department of Biology, University College of Umluj, University of Tabuk, Umluj 71491, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4284-2436","authenticated-orcid":false,"given":"Hayfa Habes","family":"Almutairi","sequence":"additional","affiliation":[{"name":"Department of Chemistry, College of Science, King Faisal University, P.O. Box 380, Al Ahsa 31982, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0227-6863","authenticated-orcid":false,"given":"Zainab H.","family":"Almansour","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, College of Science, King Faisal University, Al Ahsa 31982, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5190-0103","authenticated-orcid":false,"given":"Mir Waqas","family":"Alam","sequence":"additional","affiliation":[{"name":"Department of Physics, College of Science, King Faisal University, Al Ahsa 31982, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,4,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"9603","DOI":"10.1007\/s00210-024-03318-x","article-title":"The challenge of antimicrobial resistance (AMR): Current status and future prospects","volume":"397","author":"Ferrara","year":"2024","journal-title":"NaunynSchmiedebergs Arch. Pharmacol."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Giurazza, R., Mazza, M.C., Andini, R., Sansone, P., Pace, M.C., and Durante-Mangoni, E. (2021). Emerging treatment options for multi-drug-resistant bacterial infections. Life, 11.","DOI":"10.3390\/life11060519"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"e00031-19","DOI":"10.1128\/CMR.00031-19","article-title":"Epidemiology and treatment of multidrug-resistant and extensively drug-resistant Pseudomonas aeruginosa infections","volume":"32","author":"Horcajada","year":"2019","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"811","DOI":"10.1080\/22221751.2022.2048972","article-title":"Carbapenemase-producing Pseudomonas aeruginosa\u2014An emerging challenge","volume":"11","author":"Tenover","year":"2022","journal-title":"Emerg. Microbes Infect."},{"key":"ref_5","unstructured":"World Health Organization (2024). WHO Bacterial Priority Pathogens List, 2024: Bacterial Pathogens of Public Health Importance, to Guide Research, Development, and Strategies to Prevent and Control Antimicrobial Resistance, World Health Organization."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Kavanaugh, L.G., Hariharan, S.M., and Conn, G.L. (2025). Determination of Pseudomonas aeruginosa MexXY-OprM substrate profile in a major efflux knockout system reveals distinct antibiotic substrate classes. Microbiol. Spectr., 13.","DOI":"10.1128\/spectrum.02903-24"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Mubeen, B., Ansar, A.N., Rasool, R., Ullah, I., Imam, S.S., Alshehri, S., Ghoneim, M.M., Alzarea, S.I., Nadeem, M.S., and Kazmi, I. (2021). Nanotechnology as a novel approach in combating microbes providing an alternative to antibiotics. Antibiotics, 10.","DOI":"10.3390\/antibiotics10121473"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Avakh, A., Grant, G.D., Cheesman, M.J., Kalkundri, T., and Hall, S. (2023). The art of war with Pseudomonas aeruginosa: Targeting Mex efflux pumps directly to strategically enhance antipseudomonal drug efficacy. Antibiotics, 12.","DOI":"10.3390\/antibiotics12081304"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Hetta, H.F., Ramadan, Y.N., Al-Harbi, A.I., Ahmed, E., Battah, B., Abd Ellah, N.H., Zanetti, S., and Donadu, M.G. (2023). Nanotechnology as a promising approach to combat multidrug-resistant bacteria: A comprehensive review and future perspectives. Biomedicines, 11.","DOI":"10.3390\/biomedicines11020413"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1227","DOI":"10.2147\/IJN.S121956","article-title":"The antimicrobial activity of nanoparticles: Present situation and prospects for the future","volume":"12","author":"Wang","year":"2017","journal-title":"Int. J. Nanomed."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Mamun, M.M., Sorinolu, A.J., Munir, M., and Vejerano, E.P. (2021). Nanoantibiotics: Functions and properties at the nanoscale to combat antibiotic resistance. Front. Chem., 9.","DOI":"10.3389\/fchem.2021.687660"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Truong, L.B., Medina-Cruz, D., Mostafavi, E., and Rabiee, N. (2021). Selenium nanomaterials to combat antimicrobial resistance. Molecules, 26.","DOI":"10.3390\/molecules26123611"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Oliveira, T.P.d.S., Lima, A.K.O., and Muehlmann, L.A. (2025). An updated review of the antimicrobial potential of selenium nanoparticles and selenium-related toxicological issues. Future Pharmacol., 5.","DOI":"10.3390\/futurepharmacol5010003"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2100598","DOI":"10.1002\/adhm.202100598","article-title":"Selenium nanoparticles for biomedical applications: From development and characterization to therapeutics","volume":"10","author":"Ferro","year":"2021","journal-title":"Adv. Healthc. Mater."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1436","DOI":"10.1039\/D3SU00145H","article-title":"Bacterial-mediated selenium nanoparticles as highly selective antimicrobial agents with anticancer properties","volume":"1","author":"Truong","year":"2023","journal-title":"RSC Sustain."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"124214","DOI":"10.1016\/j.ijpharm.2024.124214","article-title":"Antibacterial and antibiofilm activities of selenium nanoparticles-antibiotic conjugates against anti-multidrug-resistant bacteria","volume":"658","author":"Ridha","year":"2024","journal-title":"Int. J. Pharm."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"106998","DOI":"10.1016\/j.micpath.2024.106998","article-title":"Antibacterial and anti-biofilm efficacy of selenium nanoparticles against Pseudomonas aeruginosa: Characterization and in vitro analysis","volume":"196","author":"Thamayandhi","year":"2024","journal-title":"Microb. Pathog."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Muenraya, P., Sawatdee, S., Srichana, T., and Atipairin, A. (2022). Silver nanoparticles conjugated with colistin enhanced the antimicrobial activity against gram-negative bacteria. Molecules, 27.","DOI":"10.3390\/molecules27185780"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"411804","DOI":"10.1155\/2022\/4118048","article-title":"Biosynthesis of selenium nanoparticles and evaluation of its antibacterial activity against Pseudomonas aeruginosa","volume":"2022","author":"Shakeri","year":"2022","journal-title":"Adv. Mater. Sci. Eng."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Kanak, K.R., Dass, R.S., and Pan, A. (2023). Anti-quorum sensing potential of selenium nanoparticles against LasI\/R, RhlI\/R, and PQS\/MvfR in Pseudomonas aeruginosa: A molecular docking approach. Front. Mol. Biosci., 10.","DOI":"10.3389\/fmolb.2023.1203672"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Abdul Hak, A., Zedan, H.H., El-Mahallawy, H.A., El-Sayyad, G.S., and Zafer, M.M. (2024). In vivo and in vitro activity of colistin-conjugated bimetallic silver-copper oxide nanoparticles against pandrug-resistant Pseudomonas aeruginosa. BMC Microbiol., 24.","DOI":"10.1186\/s12866-024-03358-6"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"868","DOI":"10.1080\/22221751.2020.1754133","article-title":"Colistin and its role in the era of antibiotic resistance: An extended review (2000\u20132019)","volume":"9","author":"Ahmed","year":"2020","journal-title":"Emerg. Microbes Infect."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"162856","DOI":"10.1016\/j.scitotenv.2023.162856","article-title":"What happens when nanoparticles encounter bacterial antibiotic resistance?","volume":"876","author":"Xu","year":"2023","journal-title":"Sci. Total Environ."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Othman, M.S., Aboelnaga, S.M., Habotta, O.A., Moneim, A.E.A., and Hussein, M.M. (2023). The potential therapeutic role of green-synthesized selenium nanoparticles using carvacrol in human breast cancer MCF-7 cells. Appl. Sci., 13.","DOI":"10.3390\/app13127039"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"7106","DOI":"10.1016\/j.biomaterials.2013.04.067","article-title":"Selective cellular uptake and induction of apoptosis of cancer-targeted selenium nanoparticles","volume":"34","author":"Huang","year":"2013","journal-title":"Biomaterials"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Ullah, A., Mu, J., Wang, F., Chan, M.W.H., Yin, X., Liao, Y., Mirani, Z.A., Sebt-E-Hassan, S., Aslam, S., and Naveed, M. (2022). Biogenic selenium nanoparticles and their anticancer effects pertaining to probiotic bacteria\u2014A review. Antioxidants, 11.","DOI":"10.3390\/antiox11101916"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Filipovi\u0107, N., U\u0161jak, D., Milenkovi\u0107, M.T., Zheng, K., Liverani, L., Boccaccini, A.R., and Stevanovi\u0107, M.M. (2021). Comparative study of the antimicrobial activity of selenium nanoparticles with different surface chemistry and structure. Front. Bioeng. Biotechnol., 8.","DOI":"10.3389\/fbioe.2020.624621"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Nile, S.H., Thombre, D., Shelar, A., Gosavi, K., Sangshetti, J., Zhang, W., Sieniawska, E., Patil, R., and Kai, G. (2023). Antifungal properties of biogenic selenium nanoparticles functionalized with nystatin for the inhibition of Candida albicans biofilm formation. Molecules, 28.","DOI":"10.3390\/molecules28041836"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"258","DOI":"10.34172\/joddd.41113","article-title":"Evaluating the anti-Candida effects of selenium nanoparticles impregnated in acrylic resins: An in vitro study","volume":"18","author":"Rostamzadeh","year":"2024","journal-title":"J. Dent. Res. Dent. Clin. Dent. Prospect."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"106462","DOI":"10.1016\/j.micpath.2023.106462","article-title":"Green synthesis of biogenic selenium nanoparticles functionalized with ginger dietary extract targeting virulence factor and biofilm formation in Candida albicans","volume":"186","author":"Thombre","year":"2024","journal-title":"Microb. Pathog."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1011","DOI":"10.1007\/s11157-021-09594-w","article-title":"Antifungal azoles and azole resistance in the environment: Current status and future perspectives\u2014A review","volume":"20","author":"Assress","year":"2021","journal-title":"Rev. Environ. Sci. Biotechnol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2697","DOI":"10.2147\/IJN.S151285","article-title":"Synergistic antifungal effect of chitosan-stabilized selenium nanoparticles synthesized by pulsed laser ablation in liquids against Candida albicans biofilms","volume":"13","author":"Lara","year":"2018","journal-title":"Int. J. Nanomed."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Zambonino, M.C., Quizhpe, E.M., Mouheb, L., Rahman, A., Agathos, S.N., and Dahoumane, S.A. (2023). Biogenic selenium nanoparticles in biomedical sciences: Properties, current trends, novel opportunities, and emerging challenges in theranostic nanomedicine. Nanomaterials, 13.","DOI":"10.3390\/nano13030424"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Ansari, J.A., Malik, J.A., Ahmed, S., Manzoor, M., Ahemad, N., and Anwar, S. (2024). Recent advances in the therapeutic applications of selenium nanoparticles. Mol. Biol. Rep., 51.","DOI":"10.1007\/s11033-024-09598-z"},{"key":"ref_35","first-page":"79","article-title":"The effect of selenium nanoparticles on the expression of MexB gene of Pseudomonas aeruginosa Isolated from wound and burn infections","volume":"22","author":"Salman","year":"2024","journal-title":"Iraqi JMS"},{"key":"ref_36","first-page":"20499361221080650","article-title":"Analysis of colistin resistance in carbapenem-resistant Enterobacterales and XDR Klebsiella pneumoniae","volume":"9","author":"Bir","year":"2022","journal-title":"Ther. Adv. Infect. Dis."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1517","DOI":"10.2147\/IDR.S299742","article-title":"Screening of antimicrobial resistance genes and epidemiological features in hospital and community-associated carbapenem-resistant Pseudomonas aeruginosa infections","volume":"14","author":"Ejder","year":"2021","journal-title":"Infect. Drug Resist."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Sentkowska, A., and Pyrzy\u0144ska, K. (2022). The influence of synthesis conditions on the antioxidant activity of selenium nanoparticles. Molecules, 27.","DOI":"10.3390\/molecules27082486"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"e41103","DOI":"10.1016\/j.heliyon.2024.e41103","article-title":"Amikacin-loaded selenium nanoparticles improved antibacterial and antibiofilm activity of amikacin against bovine mastitis-causing Staphylococcus aureus","volume":"11","author":"Asadpour","year":"2025","journal-title":"Heliyon"},{"key":"ref_40","first-page":"1455","article-title":"Green Synthesis and Characterization of Selenium Nanoparticles via Staphylococcus warneri Approach: Antimicrobial and on PhzM Pyocyanin Gene Expression in Pseudomonas aeruginosa","volume":"20","author":"Fawzi","year":"2024","journal-title":"Plasmonics"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Sans-Serramitjana, E., Gallardo-Benavente, C., Melo, F., P\u00e9rez-Donoso, J.M., Rumpel, C., Barra, P.J., Dur\u00e1n, P., and Mora, M.d.L.L. (2023). A comparative study of the synthesis and characterization of biogenic selenium nanoparticles by two contrasting endophytic selenobacteria. Microorganisms, 11.","DOI":"10.3390\/microorganisms11061600"},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Tabibi, M., Aghaei, S., Amoozegar, M.A., Nazari, R., and Zolfaghari, M.R. (2023). Characterization of green synthesized selenium nanoparticles (SeNPs) in two different indigenous halophilic bacteria. BMC Chem., 17.","DOI":"10.1186\/s13065-023-01034-w"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"118349","DOI":"10.1016\/j.carbpol.2021.118349","article-title":"Cellular uptake of chitosan and its role in antifungal action against Penicillium expansum","volume":"269","author":"Sun","year":"2021","journal-title":"Carbohydr. Polym."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"656","DOI":"10.1049\/mna2.12096","article-title":"Biosynthesis of selenium nanoparticles by Aspergillus flavus and Candida albicans for antifungal applications","volume":"16","author":"Bafghi","year":"2021","journal-title":"Micro Nano Lett."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"e7805","DOI":"10.1002\/aoc.7805","article-title":"Green Synthesis and Characterization of Apple Peel-Derived Selenium Nanoparticles for Anti-Fungal Activity and Effects of MexA Gene Expression on Efflux Pumps in Acinetobacter baumannii","volume":"39","author":"Ahmed","year":"2024","journal-title":"Appl. Organomet. Chem."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Kamnev, A.A., Dyatlova, Y.A., Kenzhegulov, O.A., Vladimirova, A.A., Mamchenkova, P.V., and Tugarova, A.V. (2021). Fourier transform infrared (FTIR) spectroscopic analyses of microbiological samples and biogenic selenium nanoparticles of microbial origin: Sample preparation effects. Molecules, 26.","DOI":"10.3390\/molecules26041146"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Kis, B., Pavel, I.Z., Avram, S., Moaca, E.A., Herrero San Juan, M., Schwiebs, A., Radeke, H.H., Muntean, D., Diaconeasa, Z., and Minda, D. (2022). Antimicrobial activity, in vitro anticancer effect (MCF-7 breast cancer cell line), antiangiogenic and immunomodulatory potentials of Populus nigra L. buds extract. BMC Complement. Med. Ther., 22.","DOI":"10.1186\/s12906-022-03526-z"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"25369","DOI":"10.1007\/s13399-023-04604-z","article-title":"Green synthesis of novel selenium nanoparticles using Crataegus monogyna extract (SeNPs@ CM) and investigation of its toxicity, antioxidant capacity, and anticancer activity against MCF-7 as a breast cancer cell line","volume":"14","author":"Barzegarparay","year":"2024","journal-title":"Biomass Convers. Biorefinery"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"52","DOI":"10.2478\/amb-2024-0078","article-title":"Antimicrobial susceptibility of fructophilic lactic acid bacteria on phzM gene of pseudomonas aeruginosa isolates from wounds infected","volume":"51","author":"Anwer","year":"2024","journal-title":"Acta Medica Bulg."},{"key":"ref_50","first-page":"508","article-title":"Expression of rhlR gene in Pseudomonas aeruginosa affected by Lactobacillus spp.","volume":"13","author":"Kasoob","year":"2022","journal-title":"J. Pharm. Negat. Results"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.femsle.2004.11.048","article-title":"Measure ment of Pseudomonas aeruginosa multidrug efflux pumps by quantitative real-time polymerase chain reaction","volume":"243","author":"Yoneda","year":"2005","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1016\/j.colsurfb.2018.06.006","article-title":"Selenium nanoparticles: A potent chemotherapeutic agent and an elucidation of its mechanism","volume":"170","author":"Menon","year":"2018","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Serov, D.A., Khabatova, V.V., Vodeneev, V., Li, R., and Gudkov, S.V. (2023). A review of the antibacterial, fungicidal, and antiviral properties of selenium nanoparticles. Materials, 16.","DOI":"10.3390\/ma16155363"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"117379","DOI":"10.1016\/j.carbpol.2020.117379","article-title":"Physicochemical stability and functional properties of selenium nanoparticles stabilized by chitosan, carrageenan, and gum Arabic","volume":"255","author":"Song","year":"2021","journal-title":"Carbohydr. Polym"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"100019","DOI":"10.1016\/j.jtemin.2022.100019","article-title":"Synthesis, optimization, and physicochemical characterization of selenium nanoparticles from polysaccharide of mangrove Rhizophora mucronata with potential bioactivities","volume":"2","author":"Jha","year":"2022","journal-title":"J. Trace Elem. Min."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"102115","DOI":"10.1016\/j.mtchem.2024.102115","article-title":"Advancing SeNP synthesis: Innovative confined environments for enhanced stability and size control","volume":"38","author":"Piacenza","year":"2024","journal-title":"Mater. Today Chem."},{"key":"ref_57","unstructured":"Grobelny, J., DelRio, F.W., Pradeep, N., Kim, D.-I., Hackley, V.A., and Cook, R.F. (2009). Size Measurement of Nanoparticles Using Atomic Force Microscopy: Version 1.1, National Cancer Institute\u2019s Nanotechnology Characterization Laboratory Assay Cascade Protocols [Internet], NIST\u2014NCL Joint Assay Protocol, PCC-6."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"7363","DOI":"10.1166\/jnn.2019.16627","article-title":"Assessment of antimicrobial features of selenium nanoparticles (SeNPs) using cyclic voltammetric strategy","volume":"19","author":"Saeed","year":"2019","journal-title":"J. Nanosci. Nanotechnol."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Pasieczna-Patkowska, S., Cichy, M., and Flieger, J. (2025). Application of Fourier transform infrared (FTIR) spectroscopy in characterization of green synthesized nanoparticles. Molecules, 30.","DOI":"10.3390\/molecules30030684"},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Zhao, Y., Xu, H., Wang, H., Chen, S., and Wang, P. (2024). Multidrug resistance in Pseudomonas aeruginosa: Genetic control mechanisms and therapeutic advances. Mol. Biomed., 5.","DOI":"10.1186\/s43556-024-00221-y"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1186\/s12934-024-02604-w","article-title":"Evaluation of the antibacterial and antibiofilm effect of mycosynthesized silver and selenium nanoparticles and their synergistic effect with antibiotics on nosocomial bacteria","volume":"24","author":"Fahmy","year":"2025","journal-title":"Microb. Cell Fact."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1022","DOI":"10.1007\/s42452-020-2835-8","article-title":"Antibiotic delivery using gold nanoparticles","volume":"2","author":"Fuller","year":"2020","journal-title":"SN Appl. Sci."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"e02033-19","DOI":"10.1128\/AAC.02033-19","article-title":"The efflux pump MexXY\/OprM contributes to the tolerance and acquired resistance of Pseudomonas aeruginosa to colistin","volume":"64","author":"Puja","year":"2020","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"100233","DOI":"10.1016\/j.microb.2025.100233","article-title":"Decoding antibiotic resistance in Pseudomonas aeruginosa: Embracing innovative therapies beyond conventional antibiotics","volume":"6","author":"Gupta","year":"2025","journal-title":"Microbe"},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Zhang, S., Wang, J., and Ahn, J. (2023). Advances in the discovery of efflux pump inhibitors as novel potentiators to control antimicrobial-resistant pathogens. Antibiotics, 12.","DOI":"10.3390\/antibiotics12091417"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"423","DOI":"10.2147\/IDR.S228089","article-title":"Acinetobacterbaumannii efflux pumps and antibiotic resistance","volume":"13","author":"Abdi","year":"2020","journal-title":"Infect. Drug Resist."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"1197","DOI":"10.1111\/jam.15638","article-title":"In vitro colistin susceptibility of pandrug-resistant Ac. baumannii is restored in the presence of selenium nanoparticles","volume":"133","year":"2022","journal-title":"J. Appl. Microbiol."},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Smitran, A., Lukovic, B., Bozic, L., Jelic, D., Jovicevic, M., Kabic, J., Kekic, D., Ranin, J., Opavski, N., and Gajic, I. (2023). Carbapenem-resistant Acinetobacter baumannii: Biofilm-associated genes, biofilm-eradication potential of disinfectants, and biofilm-inhibitory effects of selenium nanoparticles. Microorganisms, 11.","DOI":"10.3390\/microorganisms11010171"},{"key":"ref_69","doi-asserted-by":"crossref","unstructured":"Reza, A., Sutton, J.M., and Rahman, K.M. (2019). Effectiveness of efflux pump inhibitors as biofilm disruptors and resistance breakers in gram-negative (ESKAPEE) bacteria. Antibiotics, 8.","DOI":"10.20944\/preprints201910.0255.v1"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"1415","DOI":"10.1039\/D1MA00639H","article-title":"Selenium nanoparticles: A review on synthesis and biomedical applications","volume":"3","author":"Bisht","year":"2022","journal-title":"Mater. Adv."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.bmt.2023.12.001","article-title":"A review on selenium nanoparticles and their biomedical applications","volume":"6","author":"Karthik","year":"2024","journal-title":"Biomed. Technol."},{"key":"ref_72","doi-asserted-by":"crossref","unstructured":"Shakibaie, M., Salari Mohazab, N., and Ayatollahi Mousavi, S.A. (2015). Antifungal activity of selenium nanoparticles synthesized by Bacillus species Msh-1 against Aspergillus fumigatus and Candida albicans. Jundishapur. J. Microbiol., 8.","DOI":"10.5812\/jjm.26381"},{"key":"ref_73","doi-asserted-by":"crossref","unstructured":"El-Sayed, A.I.M., El-Sheekh, M.M., and Abo-Neima, S.E. (2024). Mycosynthesis of selenium nanoparticles using Penicillium tardochrysogenum as a therapeutic agent and their combination with infrared irradiation against Ehrlich carcinoma. Sci. Rep., 14.","DOI":"10.1038\/s41598-024-52982-9"},{"key":"ref_74","doi-asserted-by":"crossref","unstructured":"Slavin, Y.N., and Bach, H. (2022). Mechanisms of antifungal properties of metal nanoparticles. Nanomaterials, 12.","DOI":"10.3390\/nano12244470"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"1270","DOI":"10.1002\/jsfa.10767","article-title":"Plant-mediated synthesis of nanoparticles and their antimicrobial activity against phytopathogens","volume":"101","year":"2021","journal-title":"J. Sci. Food Agric."},{"key":"ref_76","doi-asserted-by":"crossref","unstructured":"Lazcano-Ram\u00edrez, H.G., Garza-Garc\u00eda, J.J.O., Hern\u00e1ndez-D\u00edaz, J.A., Le\u00f3n-Morales, J.M., Mac\u00edas-Sandoval, A.S., and Garc\u00eda-Morales, S. (2023). Antifungal activity of selenium nanoparticles obtained by plant-mediated synthesis. Antibiotics, 12.","DOI":"10.3390\/antibiotics12010115"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"5787","DOI":"10.2147\/IJN.S177658","article-title":"Restriction of H1N1 influenza virus infection by selenium nanoparticles loaded with ribavirin via resisting caspase-3 apoptotic pathway","volume":"13","author":"Lin","year":"2018","journal-title":"Int. J. Nanomed."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"119570","DOI":"10.1016\/j.ijpharm.2020.119570","article-title":"Optimization of inhalable liposomal powder formulations and evaluation of their in vitro drug delivery behavior in Calu-3 human lung epithelial cells","volume":"586","author":"Yu","year":"2020","journal-title":"Int. J. Pharm."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1166\/nnl.2020.3115","article-title":"Selenium nanoparticles induce cytotoxicity and apoptosis in human breast cancer (MCF-7) and liver (HepG2) cell lines","volume":"12","author":"Alkhudhayri","year":"2020","journal-title":"Nanosci. Nanotechnol. Lett."},{"key":"ref_80","doi-asserted-by":"crossref","unstructured":"Varlamova, E.G., Baimler, I.V., Gudkov, S.V., and Turovsky, E.A. (2023). Comparative study of the anticancer effects of selenium nanoparticles and selenium nanorods: Regulation of Ca2+ signaling, ER stress, and apoptosis. Appl. Sci., 13.","DOI":"10.3390\/app131910763"},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"1679","DOI":"10.1016\/j.jiph.2021.09.015","article-title":"A synergic action of colistin, imipenem, and silver nanoparticles against pandrug-resistant Acinetobacter baumannii isolated from patients","volume":"14","author":"Khaled","year":"2021","journal-title":"J. Infect. Public Health"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"5419","DOI":"10.2147\/IJN.S449185","article-title":"Modification of the antibiotic, colistin, with dextrin causes enhanced cytotoxicity and triggers apoptosis in myeloid leukemia","volume":"19","author":"Rizzo","year":"2024","journal-title":"Int. J. Nanomed."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"111968","DOI":"10.1016\/j.envres.2021.111968","article-title":"Role of nanomaterials in deactivating multiple drug resistance efflux pumps\u2014A review","volume":"204","author":"Dey","year":"2022","journal-title":"Environ. Res."},{"key":"ref_84","doi-asserted-by":"crossref","unstructured":"Chen, W., Cheng, H., and Xia, W. (2022). Progress in the surface functionalization of selenium nanoparticles and their potential application in cancer therapy. Antioxidants, 11.","DOI":"10.3390\/antiox11101965"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"122899","DOI":"10.1016\/j.lfs.2024.122899","article-title":"Nanoparticles in cancer theragnostic and drug delivery: A comprehensive review","volume":"352","author":"Jan","year":"2024","journal-title":"Life Sci."},{"key":"ref_86","doi-asserted-by":"crossref","unstructured":"Yao, Y., Zhou, Y., Liu, L., Xu, Y., Chen, Q., Wang, Y., Wu, S., Deng, Y., Zhang, J., and Shao, A. (2020). Nanoparticle-based drug delivery in cancer therapy and its role in overcoming drug resistance. Front. Mol. Biosci., 7.","DOI":"10.3389\/fmolb.2020.00193"}],"container-title":["Pharmaceutics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4923\/17\/5\/556\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:20:35Z","timestamp":1760030435000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4923\/17\/5\/556"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,24]]},"references-count":86,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2025,5]]}},"alternative-id":["pharmaceutics17050556"],"URL":"https:\/\/doi.org\/10.3390\/pharmaceutics17050556","relation":{},"ISSN":["1999-4923"],"issn-type":[{"value":"1999-4923","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,24]]}}}