{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T23:19:34Z","timestamp":1790205574114,"version":"4.1.0"},"reference-count":43,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2025,7,25]],"date-time":"2025-07-25T00:00:00Z","timestamp":1753401600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceutics"],"abstract":"<jats:p>Background\/Objectives: Chronic wounds represent a significant clinical and public health challenge due to impaired tissue repair and high associated morbidity. This study investigates the therapeutic potential of the secretome derived from human mesenchymal stem cells obtained from the pulp of deciduous teeth (hDP-MSCs) in promoting skin wound healing. Methods: After confirming the mesenchymal identity and multipotent differentiation potential of hDP-MSCs by using flow cytometry and histological staining, the effects of the secretome on human keratinocyte (HaCaT) cultures were evaluated. Results: Scratch assays, performed under high- and low-glucose conditions, demonstrated that the secretome significantly promoted keratinocyte migration and wound closure without compromising cell viability. Additionally, the secretome modulated the expression of key genes involved in inflammation and tissue regeneration, including IL-1\u03b2, TNF-\u03b1, TGF-\u03b21, and VEGF-\u03b1, in a time-dependent manner. Under inflammatory conditions induced by lipopolysaccharide, co-treatment with the secretome significantly reduced TNF-\u03b1 expression and increased TGF-\u03b21 expression, suggesting an anti-inflammatory effect. Conclusions: These findings indicate the potential of the hDP-MSC-derived secretome as a promising cell-free therapeutic strategy capable of accelerating skin regeneration and modulating the inflammatory response during the wound healing process.<\/jats:p>","DOI":"10.3390\/pharmaceutics17080961","type":"journal-article","created":{"date-parts":[[2025,7,25]],"date-time":"2025-07-25T11:38:41Z","timestamp":1753443521000},"page":"961","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["The Secretome of Human Deciduous Tooth-Derived Mesenchymal Stem Cells Enhances In Vitro Wound Healing and Modulates Inflammation"],"prefix":"10.3390","volume":"17","author":[{"given":"Thais Simi\u00e3o","family":"Pay\u00e3o","sequence":"first","affiliation":[{"name":"School of Life Sciences, Faculty of Veterinary Medicine, Pontifical Catholic University of Campinas (PUC Campinas), S\u00e3o Paulo 13060-904, Brazil"},{"name":"ImmunOncology, Pontifical Catholic University of Campinas, S\u00e3o Paulo 13060-904, Brazil"},{"name":"UroGen, National Institute of Science, Technology and Innovation in Genitourinary Cancer (INCT), Campinas, S\u00e3o Paulo 13060-904, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-8383-2675","authenticated-orcid":false,"given":"Vanessa","family":"Pellegrini","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Faculty of Veterinary Medicine, Pontifical Catholic University of Campinas (PUC Campinas), S\u00e3o Paulo 13060-904, Brazil"},{"name":"ImmunOncology, Pontifical Catholic University of Campinas, S\u00e3o Paulo 13060-904, Brazil"},{"name":"UroGen, National Institute of Science, Technology and Innovation in Genitourinary Cancer (INCT), Campinas, S\u00e3o Paulo 13060-904, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseane","family":"Morari","sequence":"additional","affiliation":[{"name":"Laboratory of Cell Signaling, Obesity and Comorbidities Research Center, School of Medical Sciences, University of Campinas, S\u00e3o Paulo 13083-864, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gisele Mara Silva","family":"Gon\u00e7alves","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Health Sciences, School of Life Sciences, Pontifical Catholic University of Campinas (PUC Campinas), S\u00e3o Paulo 13060-904, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6555-4923","authenticated-orcid":false,"given":"Maria Carolina Ximenes","family":"de Godoy","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Health Sciences, School of Life Sciences, Pontifical Catholic University of Campinas (PUC Campinas), S\u00e3o Paulo 13060-904, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4862-4328","authenticated-orcid":false,"given":"Alessandra","family":"Gambero","sequence":"additional","affiliation":[{"name":"ImmunOncology, Pontifical Catholic University of Campinas, S\u00e3o Paulo 13060-904, Brazil"},{"name":"UroGen, National Institute of Science, Technology and Innovation in Genitourinary Cancer (INCT), Campinas, S\u00e3o Paulo 13060-904, Brazil"},{"name":"Postgraduate Program in Health Sciences, School of Life Sciences, Pontifical Catholic University of Campinas (PUC Campinas), S\u00e3o Paulo 13060-904, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2092-414X","authenticated-orcid":false,"given":"Leonardo O.","family":"Reis","sequence":"additional","affiliation":[{"name":"ImmunOncology, Pontifical Catholic University of Campinas, S\u00e3o Paulo 13060-904, Brazil"},{"name":"UroGen, National Institute of Science, Technology and Innovation in Genitourinary Cancer (INCT), Campinas, S\u00e3o Paulo 13060-904, Brazil"},{"name":"Postgraduate Program in Health Sciences, School of Life Sciences, Pontifical Catholic University of Campinas (PUC Campinas), S\u00e3o Paulo 13060-904, Brazil"},{"name":"UroScience, University of Campinas, Campinas, S\u00e3o Paulo 13083-887, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L\u00edcio Augusto","family":"Velloso","sequence":"additional","affiliation":[{"name":"Laboratory of Cell Signaling, Obesity and Comorbidities Research Center, School of Medical Sciences, University of Campinas, S\u00e3o Paulo 13083-864, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7539-8477","authenticated-orcid":false,"given":"Eliana Pereira","family":"Ara\u00fajo","sequence":"additional","affiliation":[{"name":"Laboratory of Cell Signaling, Obesity and Comorbidities Research Center, School of Medical Sciences, University of Campinas, S\u00e3o Paulo 13083-864, Brazil"},{"name":"Faculty of Nursing, University of Campinas, S\u00e3o Paulo 13083-887, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L\u00edvia Bitencourt","family":"Pascoal","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Faculty of Veterinary Medicine, Pontifical Catholic University of Campinas (PUC Campinas), S\u00e3o Paulo 13060-904, Brazil"},{"name":"ImmunOncology, Pontifical Catholic University of Campinas, S\u00e3o Paulo 13060-904, Brazil"},{"name":"UroGen, National Institute of Science, Technology and Innovation in Genitourinary Cancer (INCT), Campinas, S\u00e3o Paulo 13060-904, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1159\/000528271","article-title":"Skin wound healing: Of 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