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Cannabidiol (CBD) can inhibit cell proliferation and induce apoptosis in human cancer cells. However, its low solubility in aqueous media requires solvents such as ethanol or dimethylsulfoxide that limit their dosage. Incorporating CBD into oil-in-water nanoemulsions (Nem) can improve its aqueous dispersibility. This study aimed to develop a CBD-Nem formulation and evaluate its effects on canine mammary cancer cell lines (CF41.Mg and IPC366) and non-cancer cells (MDCK). Methods: CBD-Nem was prepared with Miglyol 812 oil and Epikuron 145 V as the surfactant, and was characterized by analyzing size, morphology, zeta potential, release profile, and uptake\/internalization. Moreover, the antitumor effects of CBD-Nem were evaluated in cancer cells through viability, proliferation, cell cycle, and migration\u2013invasion assays. Results: CBD-Nem exhibited a monodisperse nanometric population (~150 nm), spherical shape, and negative zeta potential (~\u221250 mV). The in vitro release kinetics showed slow and sustained delivery at both pH 5.5 and pH 7.4. Rhodamine-Nem, as a fluorescent model of CBD-Nem, was taken up and homogenously internalized in CF41.Mg cells. CBD-Nem decreased the viability of cancer cells with a maximum effect at 50 \u00b5M and showed a lower toxicity in MDCK cells. Long-term efficacy (20 days) was evidenced by CBD-Nem at inhibiting colony formation in cancer cells. Furthermore, CBD-Nem reduced the proportion of cells in the G2-M phase, induced apoptosis, and inhibited the migration and invasion of CF41.Mg cells. Conclusions: CBD-Nem exhibited an in vitro antitumor effect, which supports its study in dogs with mammary carcinoma.<\/jats:p>","DOI":"10.3390\/pharmaceutics17080970","type":"journal-article","created":{"date-parts":[[2025,7,28]],"date-time":"2025-07-28T14:50:03Z","timestamp":1753714203000},"page":"970","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Formulation and Functional Characterization of a Cannabidiol-Loaded Nanoemulsion in Canine Mammary Carcinoma Cells"],"prefix":"10.3390","volume":"17","author":[{"given":"Francisca J.","family":"Medina","sequence":"first","affiliation":[{"name":"PhD Program in Agriculture, Forestry, and Veterinary Sciences, Universidad de Chile, Santiago 8820808, Chile"},{"name":"Centralized Laboratory for Veterinary Research (LACIV), Faculty of Veterinary and Animal Sciences, Universidad de Chile, Santiago 8320000, Chile"},{"name":"Drug Delivery Laboratory, Department of Pharmaceutical Science and Technology, Faculty of Chemical and Pharmaceutical Sciences, Universidad de Chile, Santiago 8320000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1994-2386","authenticated-orcid":false,"given":"Guillermo","family":"Velasco","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Faculty of Chemical Sciences, Universidad Complutense de Madrid and Instituto de Investigaci\u00f3n Sanitaria San Carlos (IdISSC), 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2613-4739","authenticated-orcid":false,"given":"Mar\u00eda G.","family":"Villamizar-Sarmiento","sequence":"additional","affiliation":[{"name":"Drug Delivery Laboratory, Department of Pharmaceutical Science and Technology, Faculty of Chemical and Pharmaceutical Sciences, Universidad de Chile, Santiago 8320000, Chile"},{"name":"Center of New Drugs for Hypertension and Heart Failure (CENDHY), Divisi\u00f3n de Enfermedades Cardiovasculares, Pontificia Universidad Cat\u00f3lica de Chile, Universidad de Chile, and Universidad Andr\u00e9s Bello, Santiago 8320000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0019-2404","authenticated-orcid":false,"given":"Cristian G.","family":"Torres","sequence":"additional","affiliation":[{"name":"Centralized Laboratory for Veterinary Research (LACIV), Faculty of Veterinary and Animal Sciences, Universidad de Chile, Santiago 8320000, Chile"},{"name":"Center of New Drugs for Hypertension and Heart Failure (CENDHY), Divisi\u00f3n de Enfermedades Cardiovasculares, Pontificia Universidad Cat\u00f3lica de Chile, Universidad de Chile, and Universidad Andr\u00e9s Bello, Santiago 8320000, Chile"},{"name":"Department of Clinical Sciences, Faculty of Veterinary and Animal Sciences, Universidad de Chile, Santiago 8320000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felipe A.","family":"Oyarzun-Ampuero","sequence":"additional","affiliation":[{"name":"Drug Delivery Laboratory, Department of Pharmaceutical Science and Technology, Faculty of Chemical and Pharmaceutical Sciences, Universidad de Chile, Santiago 8320000, Chile"},{"name":"Center of New Drugs for Hypertension and Heart Failure (CENDHY), Divisi\u00f3n de Enfermedades Cardiovasculares, Pontificia Universidad Cat\u00f3lica de Chile, Universidad de Chile, and Universidad Andr\u00e9s Bello, Santiago 8320000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Torres, C., Iturriaga, M., and Cruz, P. (2021). Hormonal Carcinogenesis in Canine Mammary Cancer: Molecular Mechanisms of Estradiol Involved in Malignant Progression. Animals, 11.","DOI":"10.3390\/ani11030608"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1111\/vco.12996","article-title":"Molecular and Pathobiology of Canine Mammary Tumour: Defining a Translational Model for Human Breast Cancer","volume":"22","author":"Guerreiro","year":"2024","journal-title":"Vet. Comp. Oncol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"976","DOI":"10.1111\/j.1939-1676.2008.0133.x","article-title":"Cancer incidence in pet dogs: Findings of the Animal Tumor Registry of Genoa, Italy","volume":"22","author":"Merlo","year":"2008","journal-title":"J. Vet. Intern. Med."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/j.prevetmed.2016.02.008","article-title":"Incidence of mammary tumors in the canine population living in the Veneto region (Northeastern Italy): Risk factors and similarities to human breast cancer","volume":"126","author":"Vascellari","year":"2016","journal-title":"Prev. Vet. Med."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1177\/0300985818806968","article-title":"Canine Mammary Tumors: Comparison of Classification and Grading Methods in a Survival Study","volume":"56","author":"Canadas","year":"2019","journal-title":"Vet. Pathol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1112","DOI":"10.1111\/j.1439-0531.2011.01816.x","article-title":"Canine mammary tumours, an overview","volume":"46","author":"Sleeckx","year":"2011","journal-title":"Reprod. Domest. Anim."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1016\/S0195-5616(03)00020-2","article-title":"Canine Mammary Gland Tumors","volume":"33","author":"Sorenmo","year":"2003","journal-title":"Vet. Clin. N. Am. Small Anim. Pract."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"906","DOI":"10.1016\/j.molonc.2014.12.010","article-title":"Modulation of the tumor microenvironment and inhibition of EGF\/EGFR pathway: Novel anti-tumor mechanisms of Cannabidiol in breast cancer","volume":"9","author":"Elbaz","year":"2015","journal-title":"Mol. Oncol."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Seltzer, E., Watters, A., Mackenzie, D., Granat, L., and Zhang, D. (2020). Cannabidiol (CBD) as a Promising Anti-Cancer Drug. Cancers, 12.","DOI":"10.3390\/cancers12113203"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1111\/vco.12669","article-title":"The effect of cannabidiol on canine neoplastic cell proliferation and mitogen-activated protein kinase activation during autophagy and apoptosis","volume":"19","author":"Henry","year":"2021","journal-title":"Vet. Comp. Oncol."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Heider, C.G., Itenberg, S.A., Rao, J., Ma, H., and Wu, X. (2022). Mechanisms of Cannabidiol (CBD) in Cancer Treatment: A Review. Biology, 11.","DOI":"10.3390\/biology11060817"},{"key":"ref_12","first-page":"8","article-title":"The Endocannabinoid System and its Modulation by Phytocannabinoids","volume":"692","author":"Marzo","year":"2015","journal-title":"Neurotherapeutics"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.breast.2018.06.009","article-title":"Novel mechanism of cannabidiol-induced apoptosis in breast cancer cell lines","volume":"41","author":"Sultan","year":"2018","journal-title":"Breast"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1186\/s13058-024-01910-w","article-title":"PTPN20 promotes metastasis through activating NF-kappaB signaling in triple-negative breast cancer","volume":"26","author":"Zuo","year":"2024","journal-title":"Breast Cancer Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"104837","DOI":"10.1016\/j.jddst.2023.104837","article-title":"Formulation and quality consideration of cannabidiol printed forms produced by fused-deposition modeling","volume":"87","author":"Jennotte","year":"2023","journal-title":"J. Drug Deliv. Sci. Technol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1159\/000497361","article-title":"Development of a Novel Nano-emulsion Formulation to Improve Intestinal Absorption of Cannabidiol","volume":"2","author":"Nakano","year":"2019","journal-title":"Med. Cannabis Cannabinoids"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"125511","DOI":"10.1016\/j.molliq.2024.125511","article-title":"Experimental investigation and thermodynamic modelling of cannabidiol and curcumin in different solvents","volume":"410","author":"Vidak","year":"2024","journal-title":"J. Mol. Liq."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Fraguas-S\u00e1nchez, A.I., Torres-Su\u00e1rez, A., Cohen, M., Delie, F., Bastida-Ruiz, D., Yart, L., Martin-Sabroso, C., and Fern\u00e1ndez-Carballido, A. (2020). PLGA Nanoparticles for the Intraperitoneal Administration of CBD in the Treatment of Ovarian Cancer: In Vitro and In Ovo Assessment. Pharmaceutics, 12.","DOI":"10.3390\/pharmaceutics12050439"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1111\/adb.12495","article-title":"Cannabidiol reduces ethanol consumption, motivation and relapse in mice","volume":"23","author":"Navarron","year":"2018","journal-title":"Addict. Biol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.ejpb.2018.11.020","article-title":"Lipid nanocapsules decorated and loaded with cannabidiol as targeted prolonged release carriers for glioma therapy: In vitro screening of critical parameters","volume":"134","author":"Benoit","year":"2019","journal-title":"Eur. J. Pharm. Biopharm."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1016\/j.jconrel.2018.10.030","article-title":"Synthetic cannabinoids nano-micelles for the management of triple negative breast cancer","volume":"291","author":"Greish","year":"2018","journal-title":"J. Control Release"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.heliyon.2020.e04570","article-title":"Development and characterisation of ibuprofen-loaded nanoemulsion with enhanced oral bioavailability","volume":"6","author":"Anuar","year":"2020","journal-title":"Heliyon"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1007\/s00280-019-03910-6","article-title":"Current status of nanomedicine in the chemotherapy of breast cancer","volume":"84","year":"2019","journal-title":"Cancer Chemother. Pharmacology"},{"key":"ref_24","first-page":"10","article-title":"Curcumin-loaded nanoemulsion: A new safe and effective formulation to prevent tumor reincidence and met\u00e1stasis","volume":"21","author":"Guerrero","year":"2018","journal-title":"Nanoscale"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"47565","DOI":"10.18632\/oncotarget.10206","article-title":"Activation of the orphan receptor GPR55 by lysophosphatidylinositol promotes metastasis in triple-negative breast cancer","volume":"7","author":"Andradas","year":"2016","journal-title":"Oncotarget"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Afrin, F., Chi, M., Eamens, A., Duchatel, R., Douglas, A., Schneider, J., Gedye, C., Woldu, A.S., and Dun, M.D. (2020). Can Hemp Help? Low-THC Cannabis and Non-THC Cannabinoids for the Treatment of Cancer. Cancers, 12.","DOI":"10.3390\/cancers12041033"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"106370","DOI":"10.1016\/j.foodhyd.2020.106370","article-title":"Design, development and evaluation of nanoemulsion containing avocado peel extract with anticancer potential: A novel biological active ingredient to enrich food","volume":"111","author":"Gotteland","year":"2021","journal-title":"Food Hydrocoll."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.ejpb.2021.05.023","article-title":"The key role of the drug self-aggregation ability to obtain optimal nanocarriers based on aromatic-aromatic drug-polymer interactions","volume":"166","author":"Guerrero","year":"2021","journal-title":"Eur. J. Pharm. Biopharm."},{"key":"ref_29","first-page":"574","article-title":"CBD loaded microparticles as a potential formulation to improve paclitaxel and doxorubicin-based chemotherapy in breast cancer","volume":"25","year":"2020","journal-title":"Int. J. Pharm."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"e0122277","DOI":"10.1371\/journal.pone.0122277","article-title":"Establishment and characterization of a new cell line of canine inflammatory mammary cancer: IPC-366","volume":"10","author":"Caceres","year":"2015","journal-title":"PLoS ONE"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Serrano, C., Guzm\u00e1n, S., Arias, J., and Torres, C. (2019). Melatonin decreases in vitro viability and migration of spheres derived from CF41.Mg canine mammary carcinoma cells. BMC Vet. Res., 15.","DOI":"10.1186\/s12917-019-2142-z"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2198","DOI":"10.3892\/ol.2017.6400","article-title":"Meloxicam decreases the migration and invasion of CF41.Mg canine mammary carcinoma cells","volume":"14","author":"Iturriaga","year":"2017","journal-title":"Oncol. Lett."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2315","DOI":"10.1038\/nprot.2006.339","article-title":"Clonogenic assay of cells in vitro","volume":"1","author":"Franken","year":"2006","journal-title":"Nat. Protoc."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.jconrel.2017.03.008","article-title":"Nanoemulsion: Concepts, development and applications in drug delivery","volume":"252","author":"Singh","year":"2017","journal-title":"J. Control. Release"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Mazzetti, C., Ferri, E., Pozzi, M., and Labra, M. (2020). Quantification of the content of cannabinol in commercially available e-liquids and studies on their thermal and photo-stability. Sci. Rep., 10.","DOI":"10.1038\/s41598-020-60477-6"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Millar, S., Maguire, R., Yates, A., and O\u2019sullivan, S. (2020). Towards Better Delivery of Cannabidiol (CBD). Pharmaceuticals, 13.","DOI":"10.3390\/ph13090219"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Grifoni, L., Vanti, G., Donato, R., Sacco, C., and Bilia, A.R. (2022). Promising Nanocarriers to Enhance Solubility and Bioavailability of Cannabidiol for a Plethora of Therapeutic Opportunities. Molecules, 27.","DOI":"10.3390\/molecules27186070"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Lewinska, A. (2021). Optimizing the Process Design of Oil-in-Water Nanoemulsion for Delivering Poorly Soluble Cannabidiol Oil. Processes, 9.","DOI":"10.3390\/pr9071180"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"122727","DOI":"10.1016\/j.ijpharm.2023.122727","article-title":"An update of nano-based drug delivery systems for cannabinoids: Biopharmaceutical aspects & therapeutic applications","volume":"635","author":"Lazzarotto","year":"2023","journal-title":"Int. J. Pharm."},{"key":"ref_40","first-page":"72","article-title":"Comparing microfluidics and ultrasonication as formulation methods for developing hempseed oil nanoemulsions for oral delivery applications","volume":"11","author":"Fathordoobady","year":"2021","journal-title":"Nature"},{"key":"ref_41","first-page":"7","article-title":"A New Methodology to Create Polymeric Nanocarriers ContainingHydrophilic Low Molecular-Weight Drugs: A Green StrategyProviding a Very High Drug Loading","volume":"16","author":"Guerrero","year":"2019","journal-title":"Mol. Pharm."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"123906","DOI":"10.1016\/j.molliq.2023.123906","article-title":"Colloidal nanomedicines with prolonged release of chloroquine based on interactions with aromatic polymers after mixing two liquids: From in silico simulation of nanoparticle formation to efficient in-bench scale up","volume":"395","author":"Flores","year":"2024","journal-title":"J. Mol. Liq."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"110375","DOI":"10.1016\/j.foodcont.2024.110375","article-title":"CBD products: Labeling accuracy of an obscure niche market","volume":"160","author":"Melchert","year":"2024","journal-title":"Food Control"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/S0928-0987(01)00095-1","article-title":"Modeling and comparison of dissolution profiles","volume":"13","author":"Costa","year":"2001","journal-title":"Eur. J. Pharm. Sci."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"478","DOI":"10.1016\/j.jconrel.2024.08.021","article-title":"Prolonged release from lipid nanoemulsions by modification of drug lipophilicity","volume":"374","author":"Baumann","year":"2024","journal-title":"J. Control. Release"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Duse, L., Agel, M.R., Pinnapireddy, S.R., Schafer, J., Selo, M.A., Ehrhardt, C., and Bakowsky, U. (2019). Photodynamic Therapy of Ovarian Carcinoma Cells with Curcumin-Loaded Biodegradable Polymeric Nanoparticles. Pharmaceutics, 11.","DOI":"10.3390\/pharmaceutics11060282"},{"key":"ref_47","first-page":"2191","article-title":"Parameters and characteristics governing cellular internalization and trans-barrier trafficking of nanostructures","volume":"10","author":"Murugan","year":"2015","journal-title":"Int. J. Nanomed."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Attia, M.F., Dieng, S.M., Collot, M., Klymchenko, A.S., Bouillot, C., Serra, C.A., Schmutzet, M., Er-Rafik, M., Vandamme, T.F., and Anton, N. (2017). Functionalizing nanoemulsions with carboxylates: Impact on the biodistribution and pharmacokinetics in mice. Macromol. Biosci., 17.","DOI":"10.1002\/mabi.201600471"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"5815","DOI":"10.1021\/acsomega.9b03992","article-title":"Synthesis and intracellular uptake of rhodamine-nucleolipid conjugates into a nanoemulsion vehicle","volume":"5","author":"Cunha","year":"2020","journal-title":"ACS Omega"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"2059","DOI":"10.2217\/nnm-2016-0074","article-title":"Nanometric emulsions encapsulating solid particles as alternative carriers for intracellular delivery","volume":"11","author":"Quignard","year":"2016","journal-title":"Nanomedicine"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1038\/nmat2442","article-title":"Understanding biophysicochemical interactions at the nano-bio interface","volume":"8","author":"Nel","year":"2009","journal-title":"Nat. Mater."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"2323","DOI":"10.1039\/C1CS15188F","article-title":"Toxicity of nanomaterials","volume":"41","author":"Sharifi","year":"2012","journal-title":"Chem. Soc. Rev."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Mashabela, M.D., and Kappo, A.P. (2024). Anti-Cancer and Anti-Proliferative Potential of Cannabidiol: A Cellular and Molecular Perspective. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25115659"},{"key":"ref_54","unstructured":"Laliberte, S., Hocker, S., and Mutsaers, A. (2021, January 14\u201316). Assessment of Cannabinoid Receptor Expression and Anti-neoplastic Effects of Cannabidiol (CBD) in Canine Urothelial Carcinoma Cells. Proceedings of the VCS Annual Conference, Boise, ID, USA."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1375","DOI":"10.1124\/jpet.106.105247","article-title":"Antitumor Activity of Plant Cannabinoids with Emphasis on the Effect of Cannabidiol on Human Breast Carcinoma","volume":"318","author":"Ligresti","year":"2006","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1111\/dom.13497","article-title":"Cannabinoid-1 receptor regulates mitochondrial dynamics and function in renal proximal tubular cells","volume":"21","author":"Drori","year":"2019","journal-title":"Diabetes Obes. Metab."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1111\/bph.14849","article-title":"Dual inhibition of cannabinoid CB1 receptor and inducible NOS attenuates obesity-induced chronic kidney disease","volume":"177","author":"Udi","year":"2020","journal-title":"Br. J. Pharmacol."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Pan, Z., Luo, Y., Xia, Y., Zhang, X., Qin, Y., and Liu, W. (2020). Cinobufagin induces cell cycle arrest at the S phase and promotes apoptosis in nasopharyngeal carcinoma cells. Biomed. Pharmacother., 122.","DOI":"10.1016\/j.biopha.2019.109763"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.1158\/1535-7163.MCT-10-1100","article-title":"Cannabidiol induces programmed cell death in breast cancer cells by coordinating the cross-talk between apoptosis and autophagy","volume":"10","author":"Shrivastava","year":"2011","journal-title":"Mol. Cancer Ther."},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Kiskov\u00e1, T., Mungenast, F., Suv\u00e1kov\u00e1, M., J\u00e4ger, W., and Thalhammer, T. (2019). Future Aspects for Cannabinoids in Breast Cancer Therapy. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20071673"},{"key":"ref_61","first-page":"10","article-title":"Cannabinoids and Cancer. Mini-Reviews","volume":"5","author":"Kogan","year":"2006","journal-title":"Med. Chem."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"6615","DOI":"10.1158\/0008-5472.CAN-05-4566","article-title":"\u03949-tetrahydrocannabinol inhibits cell cycle progression in human breast cancer cells through Cdc2 regulation","volume":"66","author":"Caffarel","year":"2006","journal-title":"Cancer Res."},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"D\u2019Aloia, A., Ceriani, M., Tisi, R., Stucchi, S., Sacco, E., and Costa, B. (2022). Cannabidiol Antiproliferative Effect in Triple-Negative Breast Cancer MDA-MB-231 Cells Is Modulated by Its Physical State and by IGF-1. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23137145"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"598","DOI":"10.1016\/j.bbabio.2011.07.001","article-title":"Regulation of mitochondrial respiration and apoptosis through cell signaling: Cytochrome c oxidase and cytochrome c in ischemia\/reperfusion injury and inflammation","volume":"1817","author":"Helling","year":"2012","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"2921","DOI":"10.1158\/1535-7163.MCT-07-0371","article-title":"Cannabidiol as a novel inhibitor of Id-1 gene expression in aggressive breast cancer cells","volume":"6","author":"McAllister","year":"2007","journal-title":"Mol. Cancer Ther."}],"container-title":["Pharmaceutics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4923\/17\/8\/970\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:16:42Z","timestamp":1760033802000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4923\/17\/8\/970"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,26]]},"references-count":65,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2025,8]]}},"alternative-id":["pharmaceutics17080970"],"URL":"https:\/\/doi.org\/10.3390\/pharmaceutics17080970","relation":{},"ISSN":["1999-4923"],"issn-type":[{"value":"1999-4923","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,26]]}}}