{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T22:47:28Z","timestamp":1777070848399,"version":"3.51.4"},"reference-count":36,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2025,8,12]],"date-time":"2025-08-12T00:00:00Z","timestamp":1754956800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100013129","name":"Ministry of Small and Medium-Sized Enterprises (SMEs) and Startups (MSS)","doi-asserted-by":"publisher","award":["S3400191"],"award-info":[{"award-number":["S3400191"]}],"id":[{"id":"10.13039\/501100013129","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceutics"],"abstract":"<jats:p>Background\/Objectives:\u00a0Perilla frutescens var. acuta Kudo, a member of the Lamiaceae family, has been previously reported to reduce neuroinflammation and potentially decrease A\u03b2 plaque accumulation in 5XFAD mice. In this study, we aimed to evaluate the anti-neuroinflammatory potential of a standardized 60% ethanol extract of Perilla leaves (PE), optimized for commercial application. Methods: The inflammatory response was assessed in LPS-stimulated BV2 microglial cells, and the cognitive improvement was evaluated in an AD animal model induced by intracerebroventricular injection of A\u03b2. Results: Using LPS-stimulated BV2 microglial cells and an A\u03b2-injected ICR mouse model of Alzheimer\u2019s disease, we found that PE significantly suppressed the LPS-induced production of nitric oxide and pro-inflammatory mediators, including IL-6, TNF-\u03b1, NF-\u03baB, iNOS, and COX-2, along with inhibition of JNK and p38 MAPK activation. Furthermore, PE upregulated CREB and BDNF expression. In vivo, PE administration alleviated A\u03b2-induced cognitive deficits, which were associated with reduced expression of JNK, NF-\u03baB, iNOS, and COX and increased CREB\/BDNF signaling in the hippocampus. Behavioral assessments\u2014including passive avoidance, Morris water maze, novel object recognition, and Y-maze tests\u2014confirmed the improvement in cognitive function. Conclusions: Collectively, these findings demonstrate that PE exerts significant anti-neuroinflammatory and neuroprotective effects, supporting its potential as a functional ingredient for cognitive enhancement.<\/jats:p>","DOI":"10.3390\/pharmaceutics17081045","type":"journal-article","created":{"date-parts":[[2025,8,12]],"date-time":"2025-08-12T15:51:02Z","timestamp":1755013862000},"page":"1045","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Anti-Neuroinflammation Effect of Standardized Ethanol Extract of Leaves of Perilla frutescens var. acuta on A\u03b2-Induced Alzheimer\u2019s Disease-like Mouse Model"],"prefix":"10.3390","volume":"17","author":[{"given":"Hyunji","family":"Kwon","sequence":"first","affiliation":[{"name":"Department of Advanced Translational Medicine, School of Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jihye","family":"Lee","sequence":"additional","affiliation":[{"name":"Industry Academic Cooperation Foundation, Daegu Haany University, 1 Hanuidae-ro, Gyeongsan-si 38610, Gyeongsangbuk-do, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eunhong","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, Yeungnam University, 280 Daehak-ro, Gyeongsan-si 38541, Gyeongsangbuk-do, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Somin","family":"Moon","sequence":"additional","affiliation":[{"name":"Department of Advanced Translational Medicine, School of Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eunbi","family":"Cho","sequence":"additional","affiliation":[{"name":"Department of Advanced Translational Medicine, School of Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jieun","family":"Jeon","sequence":"additional","affiliation":[{"name":"Department of Advanced Translational Medicine, School of Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A Young","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Advanced Translational Medicine, School of Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joon-Ho","family":"Hwang","sequence":"additional","affiliation":[{"name":"Borambio Co., Ltd., 119 Dandae-ro, Dongnam-gu, Cheonan 31116, Chungcheongnam, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gun Hee","family":"Cho","sequence":"additional","affiliation":[{"name":"Borambio Co., Ltd., 119 Dandae-ro, Dongnam-gu, Cheonan 31116, Chungcheongnam, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haram","family":"Kong","sequence":"additional","affiliation":[{"name":"Borambio Co., Ltd., 119 Dandae-ro, Dongnam-gu, Cheonan 31116, Chungcheongnam, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mi-Houn","family":"Park","sequence":"additional","affiliation":[{"name":"Borambio Co., Ltd., 119 Dandae-ro, Dongnam-gu, Cheonan 31116, Chungcheongnam, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sung-Kyu","family":"Kim","sequence":"additional","affiliation":[{"name":"Borambio Co., Ltd., 119 Dandae-ro, Dongnam-gu, Cheonan 31116, Chungcheongnam, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong Hyun","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Advanced Translational Medicine, School of Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea"},{"name":"Department of Pharmacology, School of Medicine, Konkuk University, 268 Chungwon-daero, Chungju-si 27478, Chungcheongbuk-do, Republic of Korea"},{"name":"Research Institute of Medical Science, Konkuk University School of Medicine, 268 Chungwon-daero, Chungju-si 27478, Chungcheongbuk-do, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ji Wook","family":"Jung","sequence":"additional","affiliation":[{"name":"Department of Cosmetic, Daegu Haany University, 1 Hanuidae-ro, Gyeongsan-si 38610, Gyeongsangbuk-do, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Aprile, D., De Marchi, F., Menegon, F., Comi, C., and Tondo, G. 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