{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,4]],"date-time":"2025-10-04T05:10:36Z","timestamp":1759554636668,"version":"build-2065373602"},"reference-count":38,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2025,10,2]],"date-time":"2025-10-02T00:00:00Z","timestamp":1759363200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"FAPESP","doi-asserted-by":"crossref","award":["2016\/06931-4"],"award-info":[{"award-number":["2016\/06931-4"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003593","name":"CNPq","doi-asserted-by":"crossref","award":["305525\/2023-9"],"award-info":[{"award-number":["305525\/2023-9"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100005942","name":"Funda\u00e7\u00e3o Butantan","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100005942","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.mdpi.com"],"crossmark-restriction":true},"short-container-title":["Pharmaceutics"],"abstract":"<jats:p>Background: Marine macroalgae has been studied by our research group as alternative sources of bioactive compounds with promising antiparasitic activity, particularly against Schistosoma mansoni. Objectives: This study aimed to employ a metabolomics-based approach to identify anthelminthic active compounds from the macroalgae Laurencia aldingensis Saito and Womersley 1974 and Laurencia dendroidea J. Agardh 1852. Methods: The algae were extracted using a dichloromethane\/methanol mixture, followed by liquid\u2013liquid partitioning and sequential chromatographic fractionation using solvents of varying polarities. The resulting fractions were tested for biological activity against adult Schistosoma mansoni worms. Detailed chemical characterization of the extracts was conducted via HPLC-DAD-MS\/MS, with subsequent data alignment and statistical analysis (Pearson correlation) to associate specific chemical compounds with the observed bioactivity. Results: Non-polar fractions (hexane and dichloromethane) exhibited significant anthelminthic activity, substantially reducing parasite viability and reproduction. Specific subfractions obtained from the dichloromethane fraction demonstrated notable activity. Metabolomic analysis revealed considerable chemical diversity, emphasizing the presence of bromophenols and halogenated sesquiterpenes, including potentially novel compounds with therapeutic potential against schistosomiasis. Conclusions: The metabolomics approach proved effective in identifying promising bioactive compounds from Laurencia spp. macroalgae with activity against S. mansoni.<\/jats:p>","DOI":"10.3390\/pharmaceutics17101294","type":"journal-article","created":{"date-parts":[[2025,10,2]],"date-time":"2025-10-02T15:07:48Z","timestamp":1759417668000},"page":"1294","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Use of Metabolomics Approach in the Discovery of Active Compounds from Macroalgae Laurencia Species Against Schistosomiasis"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2479-5325","authenticated-orcid":false,"given":"Amanda Beatriz Silva","family":"Soares","sequence":"first","affiliation":[{"name":"Laboratory of Parasitology, Butantan Institute, S\u00e3o Paulo 05503-900, SP, Brazil"},{"name":"Laboratory of Biochemistry and Molecular Biology of Marine Algae, Chemistry Institute, University of Sao Paulo, S\u00e3o Paulo 05508-090, SP, Brazil"},{"name":"Interunits Postgraduate Program in Biotechnology (PPIB), Institute of Biomedical Sciences, University of Sao Paulo, S\u00e3o Paulo 05508-000, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4871-5972","authenticated-orcid":false,"given":"Patricia Aoki","family":"Miyasato","sequence":"additional","affiliation":[{"name":"Laboratory of Parasitology, Butantan Institute, S\u00e3o Paulo 05503-900, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2027-5605","authenticated-orcid":false,"given":"Rafaela Paula","family":"de Freitas","sequence":"additional","affiliation":[{"name":"Laboratory of Parasitology, Butantan Institute, S\u00e3o Paulo 05503-900, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4430-0905","authenticated-orcid":false,"given":"Adolfo Luis Almeida","family":"Maleski","sequence":"additional","affiliation":[{"name":"Experimental Morphophysiology Laboratory, Natural and Humanities Sciences Center (CCNH), Federal University of ABC (UFABC), S\u00e3o Bernardo do Campo 09606-070, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2406-0860","authenticated-orcid":false,"given":"Daniel Carvalho","family":"Pimenta","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry, Butantan Institute, S\u00e3o Paulo 05503-900, SP, Brazil"},{"name":"Ecology and Evolution Laboratory, Butantan Institute, S\u00e3o Paulo 05503-900, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2334-1532","authenticated-orcid":false,"given":"Pio","family":"Colepicolo","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry and Molecular Biology of Marine Algae, Chemistry Institute, University of Sao Paulo, S\u00e3o Paulo 05508-090, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3309-1944","authenticated-orcid":false,"given":"Erika Mattos","family":"Stein","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry and Molecular Biology of Marine Algae, Chemistry Institute, University of Sao Paulo, S\u00e3o Paulo 05508-090, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0398-3167","authenticated-orcid":false,"given":"Arthur Ladeira","family":"Macedo","sequence":"additional","affiliation":[{"name":"Laboratory of Natural Products and Mass Spectrometry\u2014LaPNEM, Faculty of Pharmaceutical Sciences, Food and Nutrition, Federal University of Mato Grosso do Sul, Campo Grande 79070-900, MS, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1231-9441","authenticated-orcid":false,"given":"Carlos Alexandre","family":"Carollo","sequence":"additional","affiliation":[{"name":"Laboratory of Natural Products and Mass Spectrometry\u2014LaPNEM, Faculty of Pharmaceutical Sciences, Food and Nutrition, Federal University of Mato Grosso do Sul, Campo Grande 79070-900, MS, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7839-9600","authenticated-orcid":false,"given":"Eliana","family":"Nakano","sequence":"additional","affiliation":[{"name":"Laboratory of Parasitology, Butantan Institute, S\u00e3o Paulo 05503-900, SP, Brazil"},{"name":"Interunits Postgraduate Program in Biotechnology (PPIB), Institute of Biomedical 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