{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T17:12:11Z","timestamp":1793293931432,"version":"4.5.0"},"reference-count":53,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T00:00:00Z","timestamp":1761177600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Scientific Research Projects Executive Council of Gaziantep University, Department of Biology","award":["FEF.DT.24.04"],"award-info":[{"award-number":["FEF.DT.24.04"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceutics"],"abstract":"<jats:p>Background\/Objectives: Arthrospira platensis (A. platensis) is a cyanobacterium rich in bioactive compounds with proven antioxidant, antimicrobial, and stabilizing properties, making it an ideal candidate for the green synthesis of zinc oxide nanoparticles (ZnO NPs). This study aimed to synthesize ZnO NPs using A. platensis extract and to evaluate the influence of post-synthesis temperature on their physicochemical and antimicrobial properties. Methods: ZnO NPs were synthesized via a co-precipitation method using A. platensis extract, followed by post-synthesis treatments at 80 \u00b0C and 400 \u00b0C. Comprehensive characterization was performed using Ultraviolet\u2013Visible Spectroscopy (UV\u2013Vis), Fourier Transform Infrared Spectroscopy (FT\u2013IR), Field Emission Scanning Electron Microscopy (FE\u2013SEM), and Energy Dispersive X-ray Spectroscopy (EDX) to assess optical, structural, and compositional features. Antioxidant activity (DPPH assay) and antimicrobial properties against Staphylococcus aureus, Escherichia coli, and Candida albicans were also evaluated. Results: FE\u2013SEM analysis confirmed a temperature-dependent effect, with ZnO NPs synthesized at 80 \u00b0C appearing as polydispersed, irregular aggregates (45.2 \u00b1 8.6 nm), while calcination at 400 \u00b0C yielded compact, angular nanoparticles (37.1 \u00b1 6.3 nm). In contrast, pure ZnO NPs were smaller (26.4 \u00b1 4.1 nm), and A. platensis extract alone showed amorphous, irregular structures. FTIR spectra demonstrated the involvement of biomolecules in nanoparticle capping and stabilization, whereas EDX analysis revealed that higher calcination reduced organic residues and increased zinc purity. Antioxidant assays indicated a decrease in phenolic and flavonoid content with increasing temperature, leading to reduced DPPH radical scavenging activity. Antimicrobial evaluation showed superior inhibition zones (17.8\u201326.0 mm) for A. platensis-ZnO NPs compared to the crude extract, with S. aureus being most susceptible, particularly to the 400 \u00b0C nanoparticles. Conclusions: The study demonstrates that A. platensis extract provides a sustainable and efficient route for ZnO NP biosynthesis. Calcination temperature significantly affects nanoparticle morphology, biochemical composition, and antimicrobial performance. These findings highlight the potential of A. platensis-ZnO NPs as eco-friendly antimicrobial agents for biomedical, pharmaceutical, and food preservation applications.<\/jats:p>","DOI":"10.3390\/pharmaceutics17111367","type":"journal-article","created":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T09:44:30Z","timestamp":1761212670000},"page":"1367","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Influence of Calcination Temperature on the Structure and Antimicrobial Properties of Arthrospira platensis-Mediated Zinc Oxide Nanoparticles"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0102-7956","authenticated-orcid":false,"given":"Noor","family":"Akhras","sequence":"first","affiliation":[{"name":"Department of Biochemistry Science and Technology, Faculty of Arts and Science, Gaziantep University, Gaziantep 27310, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2448-4957","authenticated-orcid":false,"given":"Abuzer","family":"\u00c7elekli","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Art and Science, Gaziantep University, Gaziantep 27310, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4676-6354","authenticated-orcid":false,"given":"H\u00fcseyin","family":"Bozkurt","sequence":"additional","affiliation":[{"name":"Department of Food Engineering, Faculty of Engineering, Gaziantep University, Gaziantep 27310, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1016\/S0140-6736(21)02724-0","article-title":"Global burden of bacterial antimicrobial resistance: A systematic analysis","volume":"399","author":"Murray","year":"2022","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1038\/s41429-023-00629-8","article-title":"Antibiotics in the clinical pipeline as of December 2022","volume":"76","author":"Butler","year":"2023","journal-title":"J. Antibiot."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1080\/10410236.2014.943634","article-title":"Antibiotic resistance: A primer and call to action","volume":"30","author":"Smith","year":"2015","journal-title":"Health Commun."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Mousa, S.A., Wissa, D.A., Hassan, H.H., Ebnalwaled, A.A., and Khairy, S.A. (2024). Enhanced photocatalytic activity of green synthesized zinc oxide nanoparticles using low-cost plant extracts. Sci. Rep., 14.","DOI":"10.1038\/s41598-024-66975-1"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Lebaka, V.R., Ravi, P., Reddy, M.C., Thummala, C., and Mandal, T.K. (2025). Zinc oxide nanoparticles in modern science and technology: Multifunctional roles in healthcare, environmental remediation, and industry. Nanomaterials, 15.","DOI":"10.3390\/nano15100754"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"El-belely, E.F., Farag, M.M.S., Said, H.A., Amin, A.S., Azab, E., Gobouri, A.A., and Fouda, A. (2021). Green synthesis of zinc oxide nanoparticles using Arthrospira platensis and evaluation of their biomedical activities. Nanomaterials, 11.","DOI":"10.3390\/nano11010095"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"162","DOI":"10.5155\/eurjchem.13.2.162-167.2231","article-title":"Effect of calcination temperature on the structure and morphology of zinc oxide nanoparticles synthesized by base-catalyzed aqueous sol-gel process","volume":"13","author":"Zahra","year":"2022","journal-title":"Eur. J. Chem."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2742","DOI":"10.1007\/s42729-025-02295-1","article-title":"Ecofriendly biosynthesis of zinc oxide nanoparticles from Arthrospira platensis and their assessment for antimicrobial, antibiofilm, anticancer potency and alleviation of copper stress in Vicia faba L.","volume":"25","author":"Elsilk","year":"2025","journal-title":"J. Soil Sci. Plant Nutr."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"101","DOI":"10.34172\/ijbsm.2020.18","article-title":"Study of the physicochemical properties and anti-biofilm effects of synthesized zinc oxide nanoparticles using Artemisia plant","volume":"5","author":"Galedari","year":"2020","journal-title":"Int. J. Basic Sci. Med."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1002\/jbm.b.31615","article-title":"ZnO Nanoparticles Enhanced Antibacterial Activity of Ciprofloxacin against Staphylococcus aureus and Escherichia coli","volume":"93","author":"Banoee","year":"2010","journal-title":"J. Biomed. Mater. Res. B Appl. Biomater."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Gentscheva, G., Angelov, G., and Dobreva, D. (2023). Application of Arthrospira platensis for medicinal purposes and the food industry: A review of the literature. Life, 13.","DOI":"10.3390\/life13030845"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Al-Wathnani, H. (2012). Antibacterial activities of the extracts of cyanobacteria and green algae isolated from desert soil in Riyadh, Kingdom of Saudi Arabia. Afr. J. Biotechnol., 11.","DOI":"10.5897\/AJB11.3390"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"100033","DOI":"10.1016\/j.fnutr.2025.100033","article-title":"Bioactive compounds and health benefits of Arthrospira platensis and Chlorella vulgaris: A comprehensive review","volume":"1","author":"Kelebek","year":"2025","journal-title":"Food Nutr."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"109356","DOI":"10.1016\/j.foodres.2020.109356","article-title":"Spirulina for the food and functional food industries","volume":"137","author":"Lafarga","year":"2020","journal-title":"Food Res. Int."},{"key":"ref_15","first-page":"406","article-title":"A review on green synthesis of zinc oxide nanoparticles\u2013an eco-friendly approach","volume":"3","author":"Agarwal","year":"2017","journal-title":"Resour. Technol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1186\/s12934-024-02360-x","article-title":"Green fabrication of ZnO nanoparticles via Spirulina platensis and its efficiency against biofilm-forming pathogens","volume":"23","author":"Ahmed","year":"2024","journal-title":"Microb. Cell Fact."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"43664","DOI":"10.1021\/acsomega.3c05183","article-title":"Polymer-Assisted Coprecipitation Synthesized Zinc Oxide Nanoparticles and Their Uses for Green Chemical Synthesis via Photocatalytic Glucose Conversions","volume":"8","author":"Kaewsaenee","year":"2023","journal-title":"ACS Omega"},{"key":"ref_18","first-page":"75","article-title":"Effect of calcination temperature on the size and optical properties of synthesized ZnO nanoparticles","volume":"12","author":"Mornani","year":"2016","journal-title":"J. Ovonic Res."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"82","DOI":"10.14710\/jksa.28.2.82-87","article-title":"Effect of calcination temperature and heating rate on zinc oxide synthesis toward antibacterial properties","volume":"28","author":"Setiadji","year":"2025","journal-title":"J. Kim. Sains Apl."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1016\/S0076-6879(99)99017-1","article-title":"Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent","volume":"299","author":"Singleton","year":"1999","journal-title":"Methods Enzymol."},{"key":"ref_21","first-page":"3","article-title":"Estimation of total flavonoid content in propolis by two complementary colometric methods","volume":"10","author":"Chang","year":"2002","journal-title":"J. Food Drug Anal."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/S0023-6438(95)80008-5","article-title":"Use of a free radical method to evaluate antioxidant activity","volume":"28","author":"Cuvelier","year":"1995","journal-title":"LWT\u2014Food Sci. Technol."},{"key":"ref_23","first-page":"216","article-title":"Identification of phenolic compounds and changes in their content during processes of white wines","volume":"15","author":"Ceyhan","year":"2023","journal-title":"Carpathian J. Food Sci. Technol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1080\/17518253.2018.1547925","article-title":"Green synthesis of zinc oxide nanoparticles: A comparison","volume":"12","author":"Fakhari","year":"2019","journal-title":"Green Chem. Lett. Rev."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"233","DOI":"10.29074\/ascls.25.4.233","article-title":"Updating antimicrobial susceptibility testing methods","volume":"25","author":"Schofield","year":"2012","journal-title":"Clin. Lab. Sci. J."},{"key":"ref_26","first-page":"1368","article-title":"Antibacterial activity of ZnO nanoparticle on Gram-positive and Gram-negative bacteria","volume":"5","year":"2021","journal-title":"Afr. J. Microbiol. Res."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"484","DOI":"10.54085\/ap.2022.11.2.59","article-title":"Antioxidant capacities of crude extract and protein glycation inhibitory activities of bioactive fractions of Arthrospira platensis Gomont","volume":"11","author":"Macwan","year":"2022","journal-title":"Ann. Phytomed."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"e13972","DOI":"10.7717\/peerj.13972","article-title":"Impact of silver nanoparticles on the nutritional properties of Arthrospira platensis","volume":"10","author":"Djearamane","year":"2022","journal-title":"PeerJ"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1","DOI":"10.9734\/CSJI\/2023\/v32i4850","article-title":"Biosynthesis of ZnO nanoparticles using Spirulina platensis based on calcination temperature changes and its antioxidant activity","volume":"32","author":"Sari","year":"2023","journal-title":"Chem. Sci. Int. J."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.canlet.2017.10.041","article-title":"Apigenin: A dietary flavonoid with diverse anticancer properties","volume":"413","author":"Gajski","year":"2018","journal-title":"Cancer Lett."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Cock, I.E., and Cheesman, M.J. (2023). A review of the antimicrobial properties of cyanobacterial natural products. Molecules, 28.","DOI":"10.3390\/molecules28207127"},{"key":"ref_32","first-page":"100625","article-title":"Impact of calcination temperature on the structural and photocatalytic properties of ZnO synthesized from gum arabic for methylene blue dye removal","volume":"17","author":"Alwash","year":"2025","journal-title":"J. Hazard. Mater. Adv."},{"key":"ref_33","first-page":"53","article-title":"A critical review on zinc oxide nanoparticles: Synthesis, properties and biomedical applications","volume":"3","author":"Dey","year":"2025","journal-title":"Intell. Pharm."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"S\u00e1nchez-P\u00e9rez, D.M., Flores-Loyola, E., M\u00e1rquez-Guerrero, S.Y., Galindo-Guzman, M., and Marszalek, J.E. (2023). Green Synthesis and Characterization of Zinc Oxide Nanoparticles Using Larrea tridentata Extract and Their Impact on the In-Vitro Germination and Seedling Growth of Capsicum annuum. Sustainability, 15.","DOI":"10.3390\/su15043080"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"102434","DOI":"10.1016\/j.jksus.2022.102434","article-title":"Zinc oxide nanoparticles, biosynthesis, characterization and their potent photocatalytic degradation, and antioxidant activities","volume":"35","author":"Albarakaty","year":"2023","journal-title":"J. King Saud Univ. Sci."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Jayachandran, A.T.R., and Nair, A.S. (2021). Green synthesis and characterization of zinc oxide nanoparticles using Cayratia pedata leaf extract. Biochem. Biophys. Rep., 26.","DOI":"10.1016\/j.bbrep.2021.100995"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"093515","DOI":"10.1063\/1.2804012","article-title":"Correlation between microstructure and optical properties of ZnO nanoparticles synthesized by ball milling","volume":"102","author":"Giri","year":"2007","journal-title":"J. Appl. Phys."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Selim, Y.A., Azb, M.A., Ragab, I., and Abd El-Azim, M.H.M. (2020). Green synthesis of zinc oxide nanoparticles using aqueous extract of Deverra tortuosa and their cytotoxic activities. Sci. Rep., 10.","DOI":"10.1038\/s41598-020-60541-1"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"29541","DOI":"10.1039\/C9RA04424H","article-title":"Optical, morphological and biological analysis of zinc oxide nanoparticles (ZnO NPs) using Papaver somniferum L.","volume":"9","author":"Muhammad","year":"2019","journal-title":"RSC Adv."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Alallam, B., Doolaanea, A.A., Alfatama, M., and Lim, V. (2023). Phytofabrication and characterisation of zinc oxide nanoparticles using pure curcumin. Pharmaceuticals, 16.","DOI":"10.3390\/ph16020269"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"65","DOI":"10.5114\/bta.2021.103763","article-title":"Biosynthesis of zinc oxide nanoparticles using Fumaria parviflora extract and evaluation of their antibacterial and antioxidant activities","volume":"102","author":"Dousti","year":"2021","journal-title":"Biotechnologia"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"e39255","DOI":"10.1016\/j.heliyon.2024.e39255","article-title":"Plant-mediated synthesis of zinc oxide (ZnO) nanoparticles using Alnus nepalensis D. Don for biological applications","volume":"10","author":"Jaishi","year":"2024","journal-title":"Heliyon"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"930","DOI":"10.1007\/s10904-023-02811-9","article-title":"ZnO and C\/ZnO catalysts synthesized via plant mediated extracts for photodegradation of crystal violet and methyl orange dyes","volume":"34","author":"Farag","year":"2024","journal-title":"J. Inorg. Organomet. Polym. Mater."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"e25590","DOI":"10.1016\/j.heliyon.2024.e25590","article-title":"Study of the antimicrobial activity of zinc oxide nanostructures mediated by two morphological structures of leaf extracts of Eucalyptus radiata","volume":"10","author":"Droepenu","year":"2024","journal-title":"Heliyon"},{"key":"ref_45","unstructured":"Jalal, M., Abdelmohsen, U.R., El-Helow, A., and El-Sheekh, M.M. (2018). Anticandidal activity of bioinspired ZnO nanoparticles. J. Mycol. Fungal Biol., 3."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"4020","DOI":"10.1021\/la104825u","article-title":"Size-dependent bacterial growth inhibition and mechanism of antibacterial activity of zinc oxide nanoparticles","volume":"27","author":"Raghupathi","year":"2011","journal-title":"Langmuir"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Krishnaveni, P., Anjali, P., Renuka, S., Deivakumari, M., Saranya, V.P., Namasivayam, S.K.R., Samrat, K., and Nachiyar, C.V. (2025). Sustainable formulation of PEGylated zinc oxide nanoparticles to enhance plant growth and combat bacterial pathogens. Discov. Biotechnol., 2.","DOI":"10.1007\/s44340-025-00027-w"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1007\/s00339-015-9019-1","article-title":"Effect of calcination temperature on the properties of ZnO nanoparticles","volume":"119","author":"Kayani","year":"2015","journal-title":"Appl. Phys. A"},{"key":"ref_49","first-page":"862","article-title":"Biogenic synthesis of metallic nanoparticles and their antibacterial applications","volume":"31","author":"Patil","year":"2021","journal-title":"J. Life Sci."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"265","DOI":"10.5423\/PPJ.OA.02.2023.0035","article-title":"Antifungal activity of green and chemically synthesized ZnO nanoparticles against Alternaria citri, the causal agent citrus black rot","volume":"39","author":"Elshafie","year":"2023","journal-title":"Plant Pathol. J."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"100191","DOI":"10.1016\/j.foohum.2023.11.018","article-title":"Improving antimicrobial activity of brown propolis with incorporation of Arthrospira platensis","volume":"2","author":"Akhras","year":"2024","journal-title":"Food Humanit."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"31153","DOI":"10.1039\/D4RA04252B","article-title":"Effect of temperature on the structure, catalyst and magnetic properties of undoped zinc oxide nanoparticles: Experimental and DFT calculation","volume":"14","author":"Sharbatdaran","year":"2024","journal-title":"RSC Adv."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1007\/s13204-017-0553-3","article-title":"Effect of temperature on the morphology of ZnO nanoparticles: A comparative study","volume":"7","author":"Kamila","year":"2017","journal-title":"Appl. Nanosci."}],"container-title":["Pharmaceutics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4923\/17\/11\/1367\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T09:48:47Z","timestamp":1761212927000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4923\/17\/11\/1367"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,23]]},"references-count":53,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2025,11]]}},"alternative-id":["pharmaceutics17111367"],"URL":"https:\/\/doi.org\/10.3390\/pharmaceutics17111367","relation":{},"ISSN":["1999-4923"],"issn-type":[{"value":"1999-4923","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,23]]}}}