{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T19:15:41Z","timestamp":1790363741253,"version":"4.1.0"},"reference-count":85,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2025,11,23]],"date-time":"2025-11-23T00:00:00Z","timestamp":1763856000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100021778","name":"Agencia I+D+i","doi-asserted-by":"crossref","award":["PICT 2021-0404"],"award-info":[{"award-number":["PICT 2021-0404"]}],"id":[{"id":"10.13039\/501100021778","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100021778","name":"Agencia I+D+i","doi-asserted-by":"crossref","award":["PICT-2021-0478"],"award-info":[{"award-number":["PICT-2021-0478"]}],"id":[{"id":"10.13039\/501100021778","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100002923","name":"Consejo Nacional de Investigaciones cient\u00edficas y t\u00e9cnicas (CONICET): Proyectos de Investigaci\u00f3n Plurianuales","doi-asserted-by":"publisher","award":["2021-2023-03073"],"award-info":[{"award-number":["2021-2023-03073"]}],"id":[{"id":"10.13039\/501100002923","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Joint Canada-Israel Health Research Program","award":["IDRC-Project 109929"],"award-info":[{"award-number":["IDRC-Project 109929"]}]},{"name":"UNLP and INGEBI"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceutics"],"abstract":"<jats:p>Background\/Objectives: Chagas disease is a neglected tropical disease caused by the protozoan parasite Trypanosoma cruzi, which currently affects around 8 million people worldwide. The therapeutic arsenal against T. cruzi is so far limited to only two approved drugs, benznidazole and nifurtimox, that have considerable side effects and limited efficacy in the chronic stage of the disease. Here, we have resorted to supervised phenotypic machine learning models to explore drug repurposing opportunities and identify potential new therapeutic solutions for Chagas disease. Methods: More than 100,000 bioactivity data points were retrieved from ChEMBL and carefully curated according to the data-centric machine learning paradigm. After curation, two datasets comprising 344 compounds tested against T. cruzi Y strain trypomastigotes and 785 compounds tested against Tulahuen strain amastigotes were obtained and used to infer ensemble learning models with excellent average and early enrichment metrics in retrospective screening experiments (AUROC &gt; 0.96 and EF0.01 &gt; 58). A prospective screening campaign was then performed on DrugBank and the Drug Repurposing Hub databases, submitting eight in silico hits for experimental confirmation. Results: Six of the in silico hits confirmed their predicted trypanocidal effects. Conclusions: We have built portable meta-classifiers capable of identifying small molecules with trypanocidal activity against amastigotes, the clinically most relevant stage of T. cruzi. The predictive ability of this meta-classifier was experimentally validated.<\/jats:p>","DOI":"10.3390\/pharmaceutics17121513","type":"journal-article","created":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T09:02:07Z","timestamp":1763974927000},"page":"1513","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Application of Data-Centric Supervised Machine Learning to Predict Phenotypic Activity Against Clinically Relevant Stages of Trypanosoma cruzi"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-7348-7976","authenticated-orcid":false,"given":"Nicol\u00e1s","family":"P\u00e9rez-Mauad","sequence":"first","affiliation":[{"name":"Instituto de Qu\u00edmica y Metabolismo del F\u00e1rmaco (IQUIMEFA), CONICET-Universidad de Buenos Aires, Buenos Aires C1113, Argentina"},{"name":"Facultad de Farmacia y Bioqu\u00edmica, Universidad de Buenos Aires, Buenos Aires C1113, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucas N.","family":"Alberca","sequence":"additional","affiliation":[{"name":"Laboratorio de Investigaci\u00f3n y Desarrollo de Bioactivos (LIDeB), Departamento de Ciencias Biol\u00f3gicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata C1904, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandra C.","family":"Schoijet","sequence":"additional","affiliation":[{"name":"Signaling and Adaptive Mechanisms in Trypanosomatids Laboratory, Instituto de Investigaciones en Ingenier\u00eda Gen\u00e9tica y Biolog\u00eda Molecular \u201cDr. H\u00e9ctor N. Torres\u201d, Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas, Buenos Aires C1428, Argentina"},{"name":"Departamento de Qu\u00edmica Biol\u00f3gica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires C1428, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8302-1509","authenticated-orcid":false,"given":"Salome C.","family":"Vilchez Larrea","sequence":"additional","affiliation":[{"name":"Signaling and Adaptive Mechanisms in Trypanosomatids Laboratory, Instituto de Investigaciones en Ingenier\u00eda Gen\u00e9tica y Biolog\u00eda Molecular \u201cDr. H\u00e9ctor N. Torres\u201d, Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas, Buenos Aires C1428, Argentina"},{"name":"Departamento de Fisiolog\u00eda, Biolog\u00eda Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires C1428, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3271-6305","authenticated-orcid":false,"given":"Emilia M.","family":"Barrionuevo","sequence":"additional","affiliation":[{"name":"Laboratorio de Investigaci\u00f3n y Desarrollo de Bioactivos (LIDeB), Departamento de Ciencias Biol\u00f3gicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata C1904, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4785-8050","authenticated-orcid":false,"given":"Giuliana","family":"Muraca","sequence":"additional","affiliation":[{"name":"Laboratorio de Investigaci\u00f3n y Desarrollo de Bioactivos (LIDeB), Departamento de Ciencias Biol\u00f3gicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata C1904, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9322-5748","authenticated-orcid":false,"given":"Valeria","family":"S\u00fclsen","sequence":"additional","affiliation":[{"name":"Instituto de Qu\u00edmica y Metabolismo del F\u00e1rmaco (IQUIMEFA), CONICET-Universidad de Buenos Aires, Buenos Aires C1113, Argentina"},{"name":"Facultad de Farmacia y Bioqu\u00edmica, Universidad de Buenos Aires, Buenos Aires C1113, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2724-0421","authenticated-orcid":false,"given":"Catalina D.","family":"Alba-Soto","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones en Microbiolog\u00eda y Parasitolog\u00eda M\u00e9dica (IMPaM), CONICET-Universidad de Buenos Aires, Buenos Aires C1121, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9386-9431","authenticated-orcid":false,"given":"Guillermo D.","family":"Alonso","sequence":"additional","affiliation":[{"name":"Signaling and Adaptive Mechanisms in Trypanosomatids Laboratory, Instituto de Investigaciones en Ingenier\u00eda Gen\u00e9tica y Biolog\u00eda Molecular \u201cDr. H\u00e9ctor N. Torres\u201d, Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas, Buenos Aires C1428, Argentina"},{"name":"Departamento de Fisiolog\u00eda, Biolog\u00eda Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires C1428, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3178-826X","authenticated-orcid":false,"given":"Alan","family":"Talevi","sequence":"additional","affiliation":[{"name":"Laboratorio de Investigaci\u00f3n y Desarrollo de Bioactivos (LIDeB), Departamento de Ciencias Biol\u00f3gicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata C1904, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,23]]},"reference":[{"key":"ref_1","first-page":"100881","article-title":"The epidemiology of Chagas disease in the Americas","volume":"13","author":"Ceccarelli","year":"2024","journal-title":"Lancet Reg. 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