{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T16:50:08Z","timestamp":1774371008642,"version":"3.50.1"},"reference-count":73,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2025,12,23]],"date-time":"2025-12-23T00:00:00Z","timestamp":1766448000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"TEOXANE SA","award":["32944\/023"],"award-info":[{"award-number":["32944\/023"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceutics"],"abstract":"<jats:p>Background\/Objectives: Burn injuries pose a significant challenge due to tissue damage and impaired healing. Cell-based therapies offer promise by delivering therapeutic cells to the wound site. However, effective cell delivery remains a critical hurdle. This study investigates the potential of non-crosslinked hyaluronic acid (HA) as a simple, versatile carrier for delivering autologous keratinocytes and fibroblasts to treat early burn wounds. Methods: Primary keratinocytes and fibroblasts were isolated from uninjured adult skin. In addition, fibroblasts and adipose stem cells (ASC) from polydactyly and progenitor fibroblasts were used. Non-cross-linked HA Redensity 1\u00ae (RD1) solutions of varying concentrations were prepared and applied to various in vitro models. Cell viability, proliferation, migration, and collagen stimulation were assessed using standard assays. Additionally, cells were suspended in Redensity 1 and applied to an in vitro de-epidemalized dermis (DED) wound model to examine cell delivery and tissue reformation. Results: Preliminary data demonstrated the feasibility of using non-cross-linked HA RD1 gel as a cell carrier. RD1 gel enhanced cell viability, retention, migration, and collagen deposition. Histological analysis revealed improved cell adhesion and migration. Conclusions: This study provides valuable insight into the potential of non-cross-linked HA RD1 as a simple and effective delivery vehicle for cell therapies in early burn care. Successful translation of this approach could significantly improve clinical outcomes for burn patients.<\/jats:p>","DOI":"10.3390\/pharmaceutics18010021","type":"journal-article","created":{"date-parts":[[2025,12,24]],"date-time":"2025-12-24T09:58:34Z","timestamp":1766570314000},"page":"21","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Non-Crosslinked Hyaluronic Acid Redensity 1\u00ae Supports Cell Viability, Proliferation, and Collagen Deposition in Early Burn Management"],"prefix":"10.3390","volume":"18","author":[{"given":"Zhifeng","family":"Liao","sequence":"first","affiliation":[{"name":"Faculty of Biology and Medicine, University of Lausanne, CH-1005 Lausanne, Switzerland"},{"name":"Service of Plastic, Reconstructive, Esthetic & Hand Surgery, Lausanne University Hospital, CH-1011 Lausanne, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-7226-485X","authenticated-orcid":false,"given":"Xi","family":"Chen","sequence":"additional","affiliation":[{"name":"Faculty of Biology and Medicine, University of Lausanne, CH-1005 Lausanne, Switzerland"},{"name":"Service of Plastic, Reconstructive, Esthetic & Hand Surgery, Lausanne University Hospital, CH-1011 Lausanne, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Romain","family":"Brusini","sequence":"additional","affiliation":[{"name":"Research and Development Department, Teoxane SA, Rue de Lyon 105, CH-1203 Geneva, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4695-1816","authenticated-orcid":false,"given":"Jimmy","family":"Faivre","sequence":"additional","affiliation":[{"name":"Research and Development Department, Teoxane SA, Rue de Lyon 105, CH-1203 Geneva, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lee Ann","family":"Applegate","sequence":"additional","affiliation":[{"name":"Faculty of Biology and Medicine, University of Lausanne, CH-1005 Lausanne, Switzerland"},{"name":"Service of Plastic, Reconstructive, Esthetic & Hand Surgery, Lausanne University Hospital, CH-1011 Lausanne, Switzerland"},{"name":"Center for Applied Biotechnology and Molecular Medicine, University of Zurich, CH-8057 Zurich, Switzerland"},{"name":"Oxford OSCAR Suzhou Center, Oxford University, Suzhou 215123, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-7840-7691","authenticated-orcid":false,"given":"Killian","family":"Flegeau","sequence":"additional","affiliation":[{"name":"Research and Development Department, Teoxane SA, Rue de Lyon 105, CH-1203 Geneva, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1220-0213","authenticated-orcid":false,"given":"Nathalie","family":"Hirt-Burri","sequence":"additional","affiliation":[{"name":"Faculty of Biology and Medicine, University of Lausanne, CH-1005 Lausanne, Switzerland"},{"name":"Service of Plastic, Reconstructive, Esthetic & Hand Surgery, Lausanne University Hospital, CH-1011 Lausanne, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"538","DOI":"10.1016\/j.burns.2006.02.025","article-title":"The Use of Cultured Epithelial Autograft in the Treatment of Major Burn Wounds: Eleven Years of Clinical Experience","volume":"32","author":"Wood","year":"2006","journal-title":"Burns"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"964","DOI":"10.1016\/j.burns.2011.03.011","article-title":"Cultured epithelial autografts in massive burns: A single-center retrospective study with 63 patients","volume":"37","author":"Cirodde","year":"2011","journal-title":"Burns"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1093\/jbcr\/irab080","article-title":"Burn Center Organization and Cellular Therapy Integration: Managing Risks and Costs","volume":"42","author":"Chemali","year":"2021","journal-title":"J. Burn Care Res."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.burns.2014.05.019","article-title":"Cultured Autologous Keratinocytes in the Treatment of Large and Deep Burns: A Retrospective Study over 15 Years","volume":"41","author":"Auxenfans","year":"2015","journal-title":"Burns"},{"key":"ref_5","unstructured":"(WHO), World Health Organization (2025, September 15). Burn Injuries. Available online: https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/burns."},{"key":"ref_6","first-page":"213","article-title":"New process: Do sequential skin cell transplants cure third degree burns? Comparative study on 517 patients","volume":"31","author":"Sabeh","year":"2018","journal-title":"Ann. Burn. Fire Disasters"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1016\/j.burns.2020.04.012","article-title":"Mesenchymal Stem Cell Therapy of Acute Thermal Burns: A Systematic Review of the Effect on Inflammation and Wound Healing","volume":"47","author":"Rangatchew","year":"2021","journal-title":"Burns"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Chen, X., Laurent, A., Liao, Z., Jaccoud, S., Abdel-Sayed, P., Flahaut, M., Scaletta, C., Raffoul, W., Applegate, L.A., and Hirt-Burri, N. (2023). Cutaneous Cell Therapy Manufacturing Timeframe Rationalization: Allogeneic Off-the-Freezer Fibroblasts for Dermo-Epidermal Combined Preparations (De-Fe002-Sk2) in Burn Care. Pharmaceutics, 15.","DOI":"10.3390\/pharmaceutics15092334"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.addr.2017.06.012","article-title":"Advances in Keratinocyte Delivery in Burn Wound Care","volume":"123","author":"Chouhan","year":"2018","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1097\/01.sap.0000250647.39784.bb","article-title":"Sprayed Cultured Epithelial Autografts for Deep Dermal Burns of the Face and Neck","volume":"58","author":"Hartmann","year":"2007","journal-title":"Ann. Plast. Surg."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1016\/j.burns.2017.10.008","article-title":"Cell-Spray Auto-Grafting Technology for Deep Partial-Thickness Burns: Problems and Solutions During Clinical Implementation","volume":"44","author":"Corcos","year":"2018","journal-title":"Burns"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Ozhathil, D.K., Tay, M.W., Wolf, S.E., and Branski, L.K. (2021). A Narrative Review of the History of Skin Grafting in Burn Care. Medicina, 57.","DOI":"10.3390\/medicina57040380"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/S0305-4179(00)00116-9","article-title":"Cadaveric Allograft Discards as a Result of Positive Skin Cultures","volume":"27","author":"Obeng","year":"2001","journal-title":"Burns"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"610","DOI":"10.1046\/j.1524-4725.1999.99022.x","article-title":"Frozen Allogeneic Human Epidermal Cultured Sheets for the Cure of Complicated Leg Ulcers","volume":"25","year":"1999","journal-title":"Dermatol. Surg."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Surowiecka, A., Chrapusta, A., Klimeczek-Chrapusta, M., Korzeniowski, T., Drukala, J., and Struzyna, J. (2022). Mesenchymal Stem Cells in Burn Wound Management. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms232315339"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1111\/iwj.13831","article-title":"Effect of Stem Cell Treatment on Burn Wounds: A Systemic Review and a Meta-Analysis","volume":"20","author":"Qiao","year":"2023","journal-title":"Int. Wound J."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1926","DOI":"10.1007\/s12015-022-10341-z","article-title":"Stem Cell-Based Tissue Engineering for the Treatment of Burn Wounds: A Systematic Review of Preclinical Studies","volume":"18","author":"Lukomskyj","year":"2022","journal-title":"Stem Cell Rev. Rep."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1186\/s13287-020-01839-9","article-title":"Efficacy of Stem Cell Therapy for Burn Wounds: A Systematic Review and Meta-Analysis of Preclinical Studies","volume":"11","author":"Li","year":"2020","journal-title":"Stem Cell Res. Ther."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1355","DOI":"10.3390\/ph4101355","article-title":"Stem Cell Therapy: A New Treatment for Burns?","volume":"4","author":"Arno","year":"2011","journal-title":"Pharmaceuticals"},{"key":"ref_20","first-page":"379","article-title":"Stem Cell Therapy for Burns: Story So Far","volume":"15","author":"Aijaz","year":"2021","journal-title":"Biologics"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1359","DOI":"10.1001\/jama.294.11.1359","article-title":"Stem Cell Research: Paths to Cancer Therapies and Regenerative Medicine","volume":"294","author":"Weissman","year":"2005","journal-title":"Jama"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1089\/wound.2015.0672","article-title":"Adipose-Derived Regenerative Cell Therapy for Burn Wound Healing: A Comparison of Two Delivery Methods","volume":"5","author":"Foubert","year":"2016","journal-title":"Adv. Wound Care"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1080\/14653240410005366-1","article-title":"Isolation and Characterization of Mesenchymal Progenitor Cells from Chorionic Villi of Human Placenta","volume":"6","author":"Igura","year":"2004","journal-title":"Cytotherapy"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1002\/term.40","article-title":"Isolation and Characterization of Mesenchymal Cells from Human Fetal Membranes","volume":"1","author":"Soncini","year":"2007","journal-title":"J. Tissue Eng. Regen. Med."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"101527","DOI":"10.1016\/j.tice.2021.101527","article-title":"An Update on Stem Cells Applications in Burn Wound Healing","volume":"72","author":"Ullah","year":"2021","journal-title":"Tissue Cell"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1089\/scd.2010.0589","article-title":"Evaluation of Senescence in Mesenchymal Stem Cells Isolated from Equine Bone Marrow, Adipose Tissue, and Umbilical Cord Tissue","volume":"21","author":"Vidal","year":"2012","journal-title":"Stem Cells Dev."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"tkab002","DOI":"10.1093\/burnst\/tkab002","article-title":"Mesenchymal Stem Cell-Based Therapy for Burn Wound Healing","volume":"9","author":"Wang","year":"2021","journal-title":"Burn. Trauma"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1186\/s13287-019-1203-3","article-title":"Skin Tissue Regeneration for Burn Injury","volume":"10","author":"Shpichka","year":"2019","journal-title":"Stem Cell Res. Ther."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Laurent, A., Lin, P., Scaletta, C., Hirt-Burri, N., Michetti, M., de Buys Roessingh, A.S., Raffoul, W., She, B.R., and Applegate, L.A. (2020). Bringing Safe and Standardized Cell Therapies to Industrialized Processing for Burns and Wounds. Front. Bioeng. Biotechnol., 8.","DOI":"10.3389\/fbioe.2020.00581"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1054","DOI":"10.1089\/ten.2004.10.1054","article-title":"Stimulation of Skin Repair Is Dependent on Fibroblast Source and Presence of Extracellular Matrix","volume":"10","author":"Wang","year":"2004","journal-title":"Tissue Eng."},{"key":"ref_31","unstructured":"Gonz\u00e1lez-Mateo, G.T., and Kopytina, V. (2025). Pharmacological and Biological Strategies as Regulators of Scarless Wound Healing and Regeneration of Skin. Wound Healing\u2014Mechanisms and Pharmacological Interventions, IntechOpen."},{"key":"ref_32","first-page":"e71101","article-title":"Management of Large Full-Thickness Burns Using Kerecis Acellular Fish Skin Graft and Recell Autologous Skin Cell Suspension: A Case Report of Two Patients with Large Surface Area Burns","volume":"16","author":"Cerceo","year":"2024","journal-title":"Cureus"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1126\/science.1253836","article-title":"Advances in Skin Grafting and Treatment of Cutaneous Wounds","volume":"346","author":"Sun","year":"2014","journal-title":"Science"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Porcello, A., Laurent, A., Hirt-Burri, N., Abdel-Sayed, P., de Buys Roessingh, A., Raffoul, W., Jordan, O., All\u00e9mann, E., and Applegate, L.A. (2022). Hyaluronan-Based Hydrogels as Functional Vectors for Standardised Therapeutics in Tissue Engineering and Regenerative Medicine, CRC Press.","DOI":"10.1201\/9781003153504-8"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1800016","DOI":"10.1002\/adhm.201800016","article-title":"Engineering Pro-Regenerative Hydrogels for Scarless Wound Healing","volume":"7","author":"Sun","year":"2018","journal-title":"Adv. Healthc. Mater."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"162","DOI":"10.4103\/2321-3868.142398","article-title":"The Use of Hyaluronic Acid Based Dressings to Treat Burns: A Review","volume":"2","author":"Longinotti","year":"2014","journal-title":"Burn. Trauma"},{"key":"ref_37","first-page":"37","article-title":"Hyaluronic Acid Accelerates Re-Epithelialization and Healing of Acute Cutaneous Wounds","volume":"27","author":"Saparov","year":"2023","journal-title":"Eur. Rev. Med. Pharmacol. Sci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"2175","DOI":"10.2147\/CCID.S402409","article-title":"Clinical and Ultrasound Evaluation of Skin Quality after Subdermal Injection of Two Non-Crosslinked Hyaluronic Acid-Based Fillers","volume":"16","author":"Bezpalko","year":"2003","journal-title":"Clin. Cosmet. Investig. Dermatol."},{"key":"ref_39","first-page":"23","article-title":"In-Vivo Injection of Oligonutrients and High Molecular Weight Hyaluronic Acid\u2014Results of a Randomized Controlled Trial","volume":"3","author":"Fanian","year":"2017","journal-title":"Aesthetic Med."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"249","DOI":"10.3390\/ebj5030024","article-title":"Sustainable Primary Cell Banking for Topical Compound Cytotoxicity Assays: Protocol Validation on Novel Biocides and Antifungals for Optimized Burn Wound Care","volume":"5","author":"Liao","year":"2024","journal-title":"Eur. Burn J."},{"key":"ref_41","unstructured":"Zadnikova, P., Sinova, R., Pavlik, V., Simek, M., Safrankova, B., Hermannova, M., Nesporova, K., and Velebny, V. (2022). The Degradation of Hyaluronan in the Skin. Biomolecules, 12."},{"key":"ref_42","unstructured":"(2009). Biological Evaluation of Medical Devices\u2014Part 5: Tests for In Vitro Cytotoxicity (Standard No. ISO 10993-5:2009)."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of Relative Gene Expression Data Using Real-Time Quantitative Pcr and the 2(-Delta Delta C(T)) Method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1038\/nmeth.2089","article-title":"Nih Image to Imagej: 25 Years of Image Analysis","volume":"9","author":"Schneider","year":"2012","journal-title":"Nat. Methods"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Chen, X., Scaletta, C., Liao, Z., Laurent, A., Applegate, L.A., and Hirt-Burri, N. (2024). Optimization and Standardization of Stable De-Epidermized Dermis (Ded) Models for Functional Evaluation of Cutaneous Cell Therapies. Bioengineering, 11.","DOI":"10.3390\/bioengineering11121297"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1089\/wound.2019.1037","article-title":"Evolution of Biological Bandages as First Cover for Burn Patients","volume":"8","author":"Raffoul","year":"2019","journal-title":"Adv. Wound Care"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Al-Dourobi, K., Laurent, A., Deghayli, L., Flahaut, M., Abdel-Sayed, P., Scaletta, C., Michetti, M., Waselle, L., Simon, J.P., and Ezzi, O.E. (2021). Retrospective Evaluation of Progenitor Biological Bandage Use: A Complementary and Safe Therapeutic Management Option for Prevention of Hypertrophic Scarring in Pediatric Burn Care. Pharmaceuticals, 14.","DOI":"10.3390\/ph14030201"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Laurent, A., Rey, M., Scaletta, C., Abdel-Sayed, P., Michetti, M., Flahaut, M., Raffoul, W., de Buys Roessingh, A., Hirt-Burri, N., and Applegate, L.A. (2023). Retrospectives on Three Decades of Safe Clinical Experience with Allogeneic Dermal Progenitor Fibroblasts: High Versatility in Topical Cytotherapeutic Care. Pharmaceutics, 15.","DOI":"10.3390\/pharmaceutics15010184"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1007\/7651_2020_295","article-title":"Gmp Tiered Cell Banking of Non-Enzymatically Isolated Dermal Progenitor Fibroblasts for Allogenic Regenerative Medicine","volume":"2286","author":"Laurent","year":"2021","journal-title":"Methods Mol. Biol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1007\/7651_2020_296","article-title":"Progenitor Biological Bandages: An Authentic Swiss Tool for Safe Therapeutic Management of Burns, Ulcers, and Donor Site Grafts","volume":"2286","author":"Laurent","year":"2021","journal-title":"Methods Mol. Biol."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Chen, X., Hirt-Burri, N., Scaletta, C., Laurent, A.E., and Applegate, L.A. (2025). Bicomponent Cutaneous Cell Therapy for Early Burn Care: Manufacturing Homogeneity and Epidermis-Structuring Functions of Clinical Grade Fe002-Sk2 Allogeneic Dermal Progenitor Fibroblasts. Pharmaceutics, 17.","DOI":"10.3390\/pharmaceutics17060692"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1001\/archderm.143.2.155","article-title":"In Vivo Stimulation of De Novo Collagen Production Caused by Cross-Linked Hyaluronic Acid Dermal Filler Injections in Photodamaged Human Skin","volume":"143","author":"Wang","year":"2007","journal-title":"Arch. Dermatol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"2879","DOI":"10.1007\/s11095-014-1354-y","article-title":"Experimental and Clinical Efficacy of Two Hyaluronic Acid-Based Compounds of Different Cross-Linkage and Composition in the Rejuvenation of the Skin","volume":"33","author":"Iannitti","year":"2016","journal-title":"Pharm. Res."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"658","DOI":"10.1038\/jid.2012.364","article-title":"Enhancing Structural Support of the Dermal Microenvironment Activates Fibroblasts, Endothelial Cells, and Keratinocytes in Aged Human Skin in Vivo","volume":"133","author":"Quan","year":"2013","journal-title":"J. Invest Dermatol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1474","DOI":"10.1111\/jocd.13794","article-title":"A Hyaluronic Acid-Based Filler Reduces Lipolysis in Human Mature Adipocytes and Maintains Adherence and Lipid Accumulation of Long-Term Differentiated Human Preadipocytes","volume":"20","author":"Nadra","year":"2021","journal-title":"J. Cosmet. Dermatol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"87","DOI":"10.3727\/215517916X690486","article-title":"Stability Enhancement Using Hyaluronic Acid Gels for Delivery of Human Fetal Progenitor Tenocytes","volume":"8","author":"Grognuz","year":"2016","journal-title":"Cell Med."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"840","DOI":"10.1016\/S0140-6736(05)67107-3","article-title":"Tissue Engineered Fetal Skin Constructs for Paediatric Burns","volume":"366","author":"Hohlfeld","year":"2005","journal-title":"Lancet"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1590\/S0080-623420160000400020","article-title":"Hyaluronic Acid Covers in Burn Treatment: A Systematic Review","volume":"50","author":"Dalmedico","year":"2016","journal-title":"Rev. Esc. Enferm. USP"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1098\/rsif.2009.0403","article-title":"A Review of Tissue-Engineered Skin Bioconstructs Available for Skin Reconstruction","volume":"7","author":"Shevchenko","year":"2010","journal-title":"J. R. Soc. Interface"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1080\/14764170701774901","article-title":"The Science of Hyaluronic Acid Dermal Fillers","volume":"10","author":"Tezel","year":"2008","journal-title":"J. Cosmet. Laser Ther."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"5955","DOI":"10.1016\/j.biomaterials.2004.02.002","article-title":"A Novel Hyaluronan-Based Biomaterial (Hyaff-11) as a Scaffold for Endothelial Cells in Tissue Engineered Vascular Grafts","volume":"25","author":"Turner","year":"2004","journal-title":"Biomaterials"},{"key":"ref_62","first-page":"214","article-title":"Caustic Burn Caused by Intradermal Self Administration of Muriatic Acid for Suicidal Attempt: Optimal Wound Healing and Functional Recovery with a Non Surgical Treatment","volume":"36","author":"Fino","year":"2015","journal-title":"Il G. Di Chir. J. Ital. Surg. Assoc."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1097\/SAP.0b013e31819b3d59","article-title":"Hyalomatrix Pa in Burn Care Practice: Results from a National Retrospective Survey, 2005 to 2006","volume":"64","author":"Gravante","year":"2010","journal-title":"Ann. Plast. Surg."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/S0140-6736(05)74873-X","article-title":"Use of Hyaluronic Acid and Cultured Autologous Keratinocytes and Fibroblasts in Extensive Burns","volume":"353","author":"Harris","year":"1999","journal-title":"Lancet"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"1110","DOI":"10.1016\/j.bjps.2007.03.005","article-title":"Hyaluronic Acid: The Scientific and Clinical Evidence","volume":"60","author":"Price","year":"2007","journal-title":"J. Plast. Reconstr. Aesthet. Surg."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1111\/j.1524-475X.2012.00777.x","article-title":"Hyaluronic Acid Derivatives and Their Healing Effect on Burns, Epithelial Surgical Wounds, and Chronic Wounds: A Systematic Review and Meta-Analysis of Randomized Controlled Trials","volume":"20","author":"Voigt","year":"2012","journal-title":"Wound Repair Regen."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"S1","DOI":"10.1016\/S1361-3111(05)80001-9","article-title":"Hydrogel Dressings in the Management of a Variety of Wound Types: A Review","volume":"9","author":"Jones","year":"2005","journal-title":"J. Orthop. Nurs."},{"key":"ref_68","first-page":"747","article-title":"Antiproliferative Effect of Topic Hyaluronic Acid Gel. Study in Gingival Biopsies of Patients with Periodontal Disease","volume":"17","author":"Mesa","year":"2002","journal-title":"Histol. Histopathol."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"1391","DOI":"10.1021\/mp500676e","article-title":"Interactions of Hyaluronic Acid with the Skin and Implications for the Dermal Delivery of Biomacromolecules","volume":"12","author":"Witting","year":"2015","journal-title":"Mol. Pharm."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"1558","DOI":"10.1016\/j.actbio.2013.12.019","article-title":"Hyaluronan: A Simple Polysaccharide with Diverse Biological Functions","volume":"10","author":"Dicker","year":"2014","journal-title":"Acta. Biomater."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1002\/jbm.a.30821","article-title":"Micromolding of Photocrosslinkable Hyaluronic Acid for Cell Encapsulation and Entrapment","volume":"79","author":"Khademhosseini","year":"2006","journal-title":"J. Biomed. Mater. Res. A"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1016\/j.ijbiomac.2016.10.065","article-title":"New E-Beam-Initiated Hyaluronan Acrylate Cryogels Support Growth and Matrix Deposition by Dermal Fibroblasts","volume":"94","author":"Thones","year":"2017","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"1113","DOI":"10.1002\/jbm.a.32287","article-title":"Hydrogels Used for Cell-Based Drug Delivery","volume":"87","author":"Schmidt","year":"2008","journal-title":"J. Biomed. Mater. Res. A"}],"container-title":["Pharmaceutics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4923\/18\/1\/21\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,24]],"date-time":"2025-12-24T10:03:40Z","timestamp":1766570620000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4923\/18\/1\/21"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,23]]},"references-count":73,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,1]]}},"alternative-id":["pharmaceutics18010021"],"URL":"https:\/\/doi.org\/10.3390\/pharmaceutics18010021","relation":{},"ISSN":["1999-4923"],"issn-type":[{"value":"1999-4923","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,23]]}}}