{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T04:12:21Z","timestamp":1790655141078,"version":"4.1.0"},"reference-count":97,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T00:00:00Z","timestamp":1773187200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012190","name":"Ministry of Science and Higher Education of the Russian Federation","doi-asserted-by":"crossref","award":["075-03-2026-305"],"award-info":[{"award-number":["075-03-2026-305"]}],"id":[{"id":"10.13039\/501100012190","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100012190","name":"Ministry of Science and Higher Education of the Russian Federation","doi-asserted-by":"crossref","award":["FSMG-2025-0023"],"award-info":[{"award-number":["FSMG-2025-0023"]}],"id":[{"id":"10.13039\/501100012190","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"crossref","award":["24-14-00120"],"award-info":[{"award-number":["24-14-00120"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"crossref","award":["24-74-00040"],"award-info":[{"award-number":["24-74-00040"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceutics"],"abstract":"<jats:p>Background\/Objectives: Magnetite nanoparticles represent promising candidates for a broad spectrum of biomedical applications, ranging from in vitro diagnostic assays to in vivo imaging, hyperthermia, and targeted drug and gene delivery, with some nanoagents already approved for clinical use. A critical determinant of their functionality is the nanoparticle coating, which facilitates beneficial interactions within biological systems. In the context of tumor-targeted therapeutic delivery, key design parameters\u2014particularly surface coatings\u2014can be optimized to enhance treatment efficacy by modulating blood circulation kinetics, biodistribution, and other critical properties. However, current preclinical screening methods primarily rely on cell culture models to identify potential nanocarriers, yet these systems often poorly correlate with actual in vivo performance. This discrepancy highlights the necessity of incorporating more biologically relevant testing platforms, such as high-throughput in vivo assays. Methods: In this work, we employed an original magnetic particle quantification (MPQ) technology to systematically evaluate the blood circulation kinetics and biodistribution patterns for magnetite nanoparticles with 17 different coatings across multiple organs and tissues, including the liver, spleen, lungs, kidneys, heart, tumor, brain, peripheral blood, muscle, and bone. This methodology offers high sensitivity, user-friendly operation, and provides quantitative measurements across a broad dynamic range of nanoparticle concentrations. These advantages enabled high-throughput acquisition of precise blood circulation and biodistribution data. In addition, histological analysis was conducted to evaluate nanoparticle penetration depth within tumor tissue. Results: Here we conducted a comprehensive study of the effect of 17 different polymer-, lectin-, and small molecule-based coatings on the behavior of magnetite nanoparticles in vivo. For each type of obtained nanoparticles, we implemented passive targeting as well as magnetic targeting, the latter using an external magnetic field localized in the tumor area. Conclusions: The collected dataset provides critical insights into how surface modifications influence nanoparticle performance in complex biological systems, offering valuable guidance for optimizing therapeutic nanocarrier design.<\/jats:p>","DOI":"10.3390\/pharmaceutics18030345","type":"journal-article","created":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T09:00:37Z","timestamp":1773219637000},"page":"345","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Effect of Different Magnetite Nanoparticle Coatings on Blood Circulation, Biodistribution, Tumor Accumulation and Penetration"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3157-7158","authenticated-orcid":false,"given":"Elizaveta N.","family":"Mochalova","sequence":"first","affiliation":[{"name":"Moscow Center for Advanced Studies, 20 Kulakova St, 123592 Moscow, Russia"},{"name":"Department of Nanobiomedicine, Sirius University of Science and Technology, 1 Olimpiyskiy Ave, 354340 Sirius, Krasnodar Region, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria A.","family":"Yurchenko","sequence":"additional","affiliation":[{"name":"Moscow Center for Advanced Studies, 20 Kulakova St, 123592 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatiana S.","family":"Vorobeva","sequence":"additional","affiliation":[{"name":"Moscow Center for Advanced Studies, 20 Kulakova St, 123592 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Darina A.","family":"Maedi","sequence":"additional","affiliation":[{"name":"Moscow Center for Advanced Studies, 20 Kulakova St, 123592 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-3712-5783","authenticated-orcid":false,"given":"Nikita O.","family":"Chernov","sequence":"additional","affiliation":[{"name":"Moscow Center for Advanced Studies, 20 Kulakova St, 123592 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga A.","family":"Kolesnikova","sequence":"additional","affiliation":[{"name":"Moscow Center for Advanced Studies, 20 Kulakova St, 123592 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ekaterina D.","family":"Tereshina","sequence":"additional","affiliation":[{"name":"Moscow Center for Advanced Studies, 20 Kulakova St, 123592 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victoria O.","family":"Shipunova","sequence":"additional","affiliation":[{"name":"Moscow Center for Advanced Studies, 20 Kulakova St, 123592 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria N.","family":"Yakovtseva","sequence":"additional","affiliation":[{"name":"Moscow Center for Advanced Studies, 20 Kulakova St, 123592 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petr I.","family":"Nikitin","sequence":"additional","affiliation":[{"name":"Moscow Engineering Physics Institute, 31 Kashirskoe Shosse, 115409 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maxim P.","family":"Nikitin","sequence":"additional","affiliation":[{"name":"Moscow Center for Advanced Studies, 20 Kulakova St, 123592 Moscow, Russia"},{"name":"Department of Nanobiomedicine, Sirius University of Science and Technology, 1 Olimpiyskiy Ave, 354340 Sirius, Krasnodar Region, Russia"},{"name":"Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16\/10 Miklukho-Maklaya St, 117997 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,3,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"157","DOI":"10.2217\/17435889.1.2.157","article-title":"Magnetic nanoparticles and their applications in medicine","volume":"1","author":"Duguet","year":"2006","journal-title":"Nanomedicine"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"716","DOI":"10.1038\/nnano.2014.156","article-title":"Biocomputing based on particle disassembly","volume":"9","author":"Nikitin","year":"2014","journal-title":"Nat. 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