{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T10:31:38Z","timestamp":1785407498396,"version":"3.56.0"},"reference-count":29,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T00:00:00Z","timestamp":1778284800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100014440","name":"Ministerio de Ciencia, Innovaci\u00f3n y Universidades","doi-asserted-by":"publisher","award":["PID2020-113274RA-I00"],"award-info":[{"award-number":["PID2020-113274RA-I00"]}],"id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014440","name":"Ministerio de Ciencia, Innovaci\u00f3n y Universidades","doi-asserted-by":"publisher","award":["PID2023-151252OB-I00"],"award-info":[{"award-number":["PID2023-151252OB-I00"]}],"id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]},{"award":["PID2020-113274RA-I00"],"award-info":[{"award-number":["PID2020-113274RA-I00"]}],"id":[{"id":"https:\/\/ror.org\/05r0vyz12","id-type":"ROR","asserted-by":"publisher"}]},{"award":["PID2023-151252OB-I00"],"award-info":[{"award-number":["PID2023-151252OB-I00"]}],"id":[{"id":"https:\/\/ror.org\/05r0vyz12","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceutics"],"abstract":"<jats:p>Background: Candida tropicalis is a pathogenic yeast species responsible for infections within the Candida genus and is identified as the most virulent species after C. albicans, partly due to its ability to form biofilms. Objective: This study analyzes the antifungal efficacy of a newly synthesized dendron, BD132 dendron, against C. tropicalis. Results: The compound showed a strong antifungal activity with promising minimum inhibitory concentration (MIC) and minimum biofilm inhibitory concentration (MBIC) values. Combination therapy with AgNO3 and amphotericin B showed additive and synergistic effects, respectively, enhancing antifungal efficacy and potentially reducing cytotoxicity. The dendron did not alter key enzyme activities, and scanning electron microscopy revealed significant morphological alterations, including increased cell size and surface damage, indicating membrane disruption. In addition, the BD132 dendron did not induce resistance, and stability studies indicated a slight MIC decrease at 4 \u00b0C and \u221220 \u00b0C after 15 days, with stable minimum fungicidal concentration (MFC), suggesting potential for long-term use. Conclusions: These findings highlight the potential of this dendron in combination therapies to treat C. tropicalis infections.<\/jats:p>","DOI":"10.3390\/pharmaceutics18050583","type":"journal-article","created":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T15:45:34Z","timestamp":1779291934000},"page":"583","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effect of the BD132 Dendron Against Candida tropicalis: Inhibition of Biofilm Formation and Enzymatic and Structural Alterations"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4381-6312","authenticated-orcid":false,"given":"Elo\u00edsa","family":"Garc\u00eda-Porcel","sequence":"first","affiliation":[{"name":"Department of Biomedicine and Biotechnology, Faculty of Pharmacy, Universidad de Alcal\u00e1, 28805 Alcal\u00e1 de Henares, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6983-1895","authenticated-orcid":false,"given":"Natalia","family":"G\u00f3mez-Casanova","sequence":"additional","affiliation":[{"name":"Department of Biomedicine and Biotechnology, Faculty of Pharmacy, Universidad de Alcal\u00e1, 28805 Alcal\u00e1 de Henares, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4870-8978","authenticated-orcid":false,"given":"Jorge","family":"P\u00e9rez-Serrano","sequence":"additional","affiliation":[{"name":"Department of Biomedicine and Biotechnology, Faculty of Pharmacy, Universidad de Alcal\u00e1, 28805 Alcal\u00e1 de Henares, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2162-3907","authenticated-orcid":false,"given":"Jos\u00e9 Luis","family":"Copa-Pati\u00f1o","sequence":"additional","affiliation":[{"name":"Department of Biomedicine and Biotechnology, Faculty of Pharmacy, Universidad de Alcal\u00e1, 28805 Alcal\u00e1 de Henares, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6984-1965","authenticated-orcid":false,"given":"Irene","family":"Heredero-Bermejo","sequence":"additional","affiliation":[{"name":"Department of Biomedicine and Biotechnology, Faculty of Pharmacy, Universidad de Alcal\u00e1, 28805 Alcal\u00e1 de Henares, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,5,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3390","DOI":"10.1021\/acs.chemrev.0c00199","article-title":"Antifungal Drug Resistance: Molecular Mechanisms in Candida albicans and Beyond","volume":"121","author":"Lee","year":"2021","journal-title":"Chem. Rev."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Karpi\u0144ski, T.M., O\u017carowski, M., Seremak-Mrozikiewicz, A., and Wolski, H. (2023). Anti-Candida and Antibiofilm Activity of Selected Lamiaceae Essential Oils. Front. Biosci. Landmark Ed., 28.","DOI":"10.31083\/j.fbl2802028"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Du, H., Bing, J., Hu, T., Ennis, C.L., Nobile, C.J., and Huang, G. (2020). Candida auris: Epidemiology, biology, antifungal resistance, and virulence. PLoS Pathog., 16.","DOI":"10.1371\/journal.ppat.1008921"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Nile, S.H., Thombre, D., Shelar, A., Gosavi, K., Sangshetti, J., Zhang, W., Sieniawska, E., Patil, R., and Kai, G. (2023). Antifungal Properties of Biogenic Selenium Nanoparticles Functionalized with Nystatin for the Inhibition of Candida albicans Biofilm Formation. Molecules, 28.","DOI":"10.3390\/molecules28041836"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"456878","DOI":"10.1155\/2014\/456878","article-title":"Virulence Factors Contributing to Pathogenicity of Candida tropicalis and Its Antifungal Susceptibility Profile","volume":"2014","author":"Deorukhkar","year":"2014","journal-title":"Int. J. Microbiol."},{"key":"ref_6","first-page":"163","article-title":"Evaluation of virulence factors and azole resistance mechanisms of Candida tropicalis isolates from head and neck cancer patients with OPC","volume":"15","author":"Jahanshiri","year":"2023","journal-title":"Iran. J. Microbiol."},{"key":"ref_7","unstructured":"World Health Organization (2022). WHO Fungal Priority Pathogens List to Guide Research, Development and Public Health Action, World Health Organization."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"El-Kholy, M.A., Helaly, G.F., El Ghazzawi, E.F., El-Sawaf, G., and Shawky, S.M. (2021). Virulence Factors and Antifungal Susceptibility Profile of C. tropicalis Isolated from Various Clinical Specimens in Alexandria, Egypt. J. Fungi, 7.","DOI":"10.3390\/jof7050351"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"847","DOI":"10.1007\/s11046-021-00580-y","article-title":"Virulence Factors and Azole-Resistant Mechanism of Candida tropicalis Isolated from Candidemia","volume":"186","author":"Sasani","year":"2021","journal-title":"Mycopathologia"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"102237","DOI":"10.1016\/j.mib.2022.102237","article-title":"Antifungal drug-resistance mechanisms in Candida biofilms","volume":"71","author":"Kaur","year":"2023","journal-title":"Curr. Opin. Microbiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1093\/mmy\/myaa046","article-title":"Novel antifungal activity of oligostyrylbenzenes compounds on Candida tropicalis biofilms","volume":"59","author":"Quinteros","year":"2021","journal-title":"Med. Mycol."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Judan Cruz, K.G., Alfonso, E.D., Fernando, S.I.D., and Watanabe, K. (2021). Candida albicans Biofilm Inhibition by Ethnobotanicals and Ethnobotanically-Synthesized Gold Nanoparticles. Front. Microbiol., 12.","DOI":"10.3389\/fmicb.2021.665113"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Zuza-Alves, D.L., Silva-Rocha, W.P., and Chaves, G.M. (2017). An Update on Candida tropicalis Based on Basic and Clinical Approaches. Front. Microbiol., 8.","DOI":"10.3389\/fmicb.2017.01927"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Mroczy\u0144ska, M., and Brillowska-D\u0105browska, A. (2021). Virulence of Clinical Candida Isolates. Pathogens, 10.","DOI":"10.3390\/pathogens10040466"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Gao, S.S., Zhao, I.S., Duffin, S., Duangthip, D., Lo, E.C.M., and Chu, C.H. (2018). Revitalising Silver Nitrate for Caries Management. Int. J. Environ. Res. Public Health, 15.","DOI":"10.3390\/ijerph15010080"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"101","DOI":"10.4103\/JIOH.JIOH_143_21","article-title":"Antimicrobial Activity of 0.05 N and 0.1 N Silver Nitrate Mouthwash against Streptococcus Mutans and Candida albicans: An: In vitro: Study","volume":"14","author":"Thilakan","year":"2022","journal-title":"J. Int. Oral Health"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Uroro, E.O., Bright, R., Hayles, A., and Vasilev, K. (2022). Lipase-Responsive Amphotericin B Loaded PCL Nanoparticles for Antifungal Therapies. Nanomaterials, 13.","DOI":"10.3390\/nano13010155"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"G\u00f3mez-Casanova, N., Lozano-Cruz, T., Soliveri, J., Gomez, R., Ortega, P., Copa-Pati\u00f1o, J.L., and Heredero-Bermejo, I. (2021). Eradication of Candida albicans biofilm viability: In vitro combination therapy of cationic carbosilane dendrons derived from 4-phenylbutyric acid with agno3 and edta. J. Fungi, 7.","DOI":"10.3390\/jof7070574"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"e3226","DOI":"10.1002\/psc.3226","article-title":"Peptide dendrimers as antifungal agents and carriers for potential antifungal agent\u2014N3-(4-methoxyfumaroyl)-(S)-2,3-diaminopropanoic acid\u2014Synthesis and antimicrobial activity","volume":"26","author":"Stolarska","year":"2020","journal-title":"J. Pept. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"38900","DOI":"10.1038\/s41598-025-22695-8","article-title":"Antibiofilm activity of cationic carbosilane BD132 dendron and its synergistic effect with amphotericin B against Candida spp.","volume":"15","year":"2025","journal-title":"Sci. Rep."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"E246","DOI":"10.1111\/j.1469-0691.2012.03880.x","article-title":"EUCAST technical note on the EUCAST definitive document EDef 7.2: Method for the determination of broth dilution minimum inhibitory concentrations of antifungal agents for yeasts EDef 7.2 (EUCAST-AFST)","volume":"18","author":"Arendrup","year":"2012","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_22","unstructured":"Askun, T. (2023). Novel Treatment Approach against Candida spp.: Evaluation of Antifungal and Antibiofilm In Vitro Activity of Dendritic Molecules. Candida Candidiasis, IntechOpen."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Heredero-Bermejo, I., G\u00f3mez-Casanova, N., Quintana, S., Soliveri, J., de la Mata, F.J., P\u00e9rez-Serrano, J., S\u00e1nchez-Nieves, J., and Copa-Pati\u00f1o, J.L. (2020). In Vitro Activity of Carbosilane Cationic Dendritic Molecules on Prevention and Treatment of Candida albicans Biofilms. Pharmaceutics, 12.","DOI":"10.3390\/pharmaceutics12100918"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"4071","DOI":"10.1128\/AAC.00410-07","article-title":"In Vitro and In Vivo Activities of Novel Fluoroquinolones Alone and in Combination with Clarithromycin against Clinically Isolated Mycobacterium avium Complex Strains in Japan","volume":"51","author":"Kohno","year":"2007","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"207","DOI":"10.3109\/13693780902801242","article-title":"Enzymatic activities of Candida tropicalis isolated from hospitalized patients","volume":"48","author":"Blanco","year":"2010","journal-title":"Med. Mycol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"7022","DOI":"10.1039\/C5RA25901K","article-title":"Structure\u2013activity relationship study of cationic carbosilane dendritic systems as antibacterial agents","volume":"6","author":"Soliveri","year":"2016","journal-title":"RSC Adv."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Hamida, R.S., Ali, M.A., Goda, D.A., and Redhwan, A. (2021). Anticandidal Potential of Two Cyanobacteria-Synthesized Silver Nanoparticles: Effects on Growth, Cell Morphology, and Key Virulence Attributes of Candida albicans. Pharmaceutics, 13.","DOI":"10.3390\/pharmaceutics13101688"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1111\/j.1439-0507.2009.01793.x","article-title":"Enhancement of Secretory Aspartyl Protease production in biofilms of Candida albicans exposed to sub-inhibitory concentrations of fluconazole","volume":"54","author":"Mores","year":"2011","journal-title":"Mycoses"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1797","DOI":"10.1007\/s10096-020-03912-w","article-title":"Mechanism of Candida pathogenesis: Revisiting the vital drivers","volume":"39","author":"Mba","year":"2020","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."}],"container-title":["Pharmaceutics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4923\/18\/5\/583\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T15:48:22Z","timestamp":1779292102000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4923\/18\/5\/583"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,9]]},"references-count":29,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2026,5]]}},"alternative-id":["pharmaceutics18050583"],"URL":"https:\/\/doi.org\/10.3390\/pharmaceutics18050583","relation":{},"ISSN":["1999-4923"],"issn-type":[{"value":"1999-4923","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,9]]}}}