{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T18:42:30Z","timestamp":1785436950181,"version":"3.56.0"},"reference-count":68,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T00:00:00Z","timestamp":1783728000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"National Council for Scientific and Technological Development","doi-asserted-by":"crossref","award":["CNPq #445132\/2023-0"],"award-info":[{"award-number":["CNPq #445132\/2023-0"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]},{"award":["CNPq #445132\/2023-0"],"award-info":[{"award-number":["CNPq #445132\/2023-0"]}],"id":[{"id":"https:\/\/ror.org\/03swz6y49","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Brazilian Innovation Agency","award":["FINEP #1099\/24"],"award-info":[{"award-number":["FINEP #1099\/24"]}]},{"name":"North-Rio-Grandense Foundation for Research and Culture","award":["FUNPEC #23077.187338\/2025-35"],"award-info":[{"award-number":["FUNPEC #23077.187338\/2025-35"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceutics"],"abstract":"<jats:p>Medicinal cannabis has gained increasing attention from both the scientific community and clinical practice, due to the therapeutic potential of its major phytocannabinoids, particularly cannabidiol (CBD) and \u03949-tetrahydrocannabinol (THC), for pain management. This review compiled and analyzed the available evidence regarding the antinociceptive effects of nanoencapsulated cannabinoids compared to free compounds. The published works have explored some pharmaceutical formulations and administration routes on different acute, chronic and neuropathic pain experimental models. The findings indicated that cannabinoids exhibited promising analgesic effects, while nanoencapsulation could enhance its stability and bioavailability. Despite these advances, the number of reports investigating nanostructured cannabinoid-based systems remains limited, with a predominance of preclinical research. A recurrent lack of structural information and quality control data for such works was also noted. Furthermore, there were not identified any research regarding the nanoencapsulation of full-spectrum cannabis oils or whole cannabis extracts, highlighting a significant gap in the current literature. Overall, nanoencapsulation emerges as a versatile strategy to overcome the intrinsic limitations of cannabinoids and expand its clinical applicability for pain treatment. Nevertheless, further efforts are required to determine standardized methodologies, facilitating the translation of preclinical findings into clinical practice, in order to provide stable, safe, effective and more accessible cannabinoid-based therapies.<\/jats:p>","DOI":"10.3390\/pharmaceutics18070844","type":"journal-article","created":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T09:51:09Z","timestamp":1783936269000},"page":"844","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Cannabis-Based Nanolipid Formulations for Pain Management"],"prefix":"10.3390","volume":"18","author":[{"given":"Ana Clara Santiago","family":"Bastos","sequence":"first","affiliation":[{"name":"Department of Pharmacy, Faculty of Pharmacy, Federal University of Rio Grande do Norte, Natal 59012-570, RN, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arissa De Oliveira","family":"Sato","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology, Federal University of Uberl\u00e2ndia, Uberl\u00e2ndia 38405-319, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luana Carvalho de","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Faculty of Pharmacy, Federal University of Rio Grande do Norte, Natal 59012-570, RN, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7623-4485","authenticated-orcid":false,"given":"Fernanda Nervo","family":"Raffin","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Faculty of Pharmacy, Federal University of Rio Grande do Norte, Natal 59012-570, RN, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T\u00falio F. 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Rep."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"124391","DOI":"10.1016\/j.lfs.2026.124391","article-title":"From Nociception to Therapy: The Expanding Role of TMEM Proteins in Pain","volume":"396","author":"Kappara","year":"2026","journal-title":"Life Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1976","DOI":"10.1097\/j.pain.0000000000001939","article-title":"The Revised International Association for the Study of Pain Definition of Pain: Concepts, Challenges, and Compromises","volume":"161","author":"Raja","year":"2020","journal-title":"Pain"},{"key":"ref_4","first-page":"100589","article-title":"Beyond the High: Therapeutic Insights into Cannabis sativa and Endocannabinoid Control of Pain","volume":"10","author":"Sahoo","year":"2026","journal-title":"Pharmacol. Res.-Nat. Prod."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"20190290","DOI":"10.1098\/rstb.2019.0290","article-title":"Evolution of Nociception and Pain: Evidence from Fish Models","volume":"374","author":"Sneddon","year":"2019","journal-title":"Phil. Trans. R. Soc. B"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1016\/j.bjae.2025.04.008","article-title":"Neurophysiology of Acute and Chronic Pain: From Genes to Pain Symptoms","volume":"25","author":"Poisbeau","year":"2025","journal-title":"BJA Educ."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"105388","DOI":"10.1016\/j.jpain.2025.105388","article-title":"Methods to Discriminate between Nociceptive, Neuropathic and Nociplastic in Children & Adolescents: A Systematic Review of Psychometric Properties and Feasibility","volume":"31","author":"Khillan","year":"2025","journal-title":"J. Pain"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"100537","DOI":"10.1016\/j.bjpt.2023.100537","article-title":"Phenotyping Nociceptive, Neuropathic, and Nociplastic Pain: Who, How, & Why?","volume":"27","author":"Nijs","year":"2023","journal-title":"Braz. J. Phys. Ther."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2639","DOI":"10.2147\/DDDT.S417051","article-title":"Recent Research Advances in Nano-Based Drug Delivery Systems for Local Anesthetics","volume":"17","author":"Ma","year":"2023","journal-title":"Drug Des. Dev. Ther."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Paul, A.K., Smith, C.M., Rahmatullah, M., Nissapatorn, V., Wilairatana, P., Spetea, M., Gueven, N., and Dietis, N. (2021). Opioid Analgesia and Opioid-Induced Adverse Effects: A Review. Pharmaceuticals, 14.","DOI":"10.3390\/ph14111091"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Farinon, B., Molinari, R., Costantini, L., and Merendino, N. (2020). The Seed of Industrial Hemp (Cannabis sativa L.): Nutritional Quality and Potential Functionality for Human Health and Nutrition. Nutrients, 12.","DOI":"10.3390\/nu12071935"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Viskovi\u0107, J., Zheljazkov, V.D., Sikora, V., Noller, J., Latkovi\u0107, D., Ocamb, C.M., and Koren, A. (2023). Industrial Hemp (Cannabis sativa L.) Agronomy and Utilization: A Review. Agronomy, 13.","DOI":"10.3390\/agronomy13030931"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1273","DOI":"10.1007\/s11101-021-09780-2","article-title":"Comprehensive Inventory of Cannabinoids in Cannabis sativa L.: Can We Connect Genotype and Chemotype?","volume":"21","author":"Lange","year":"2022","journal-title":"Phytochem. Rev."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Blebea, N.M., Pricopie, A.I., Vlad, R.-A., and Hancu, G. (2024). Phytocannabinoids: Exploring Pharmacological Profiles and Their Impact on Therapeutical Use. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25084204"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1038\/s41467-024-55808-4","article-title":"Structural Basis of THC Analog Activity at the Cannabinoid 1 Receptor","volume":"16","author":"Thorsen","year":"2025","journal-title":"Nat. Commun."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Pressman, P., Hayes, A.W., Hoeng, J., Latino, D.A.R.S., Mazurov, A., Schlage, W.K., and Rana, A. (2024). \u03949-Tetrahydrocannabinol (THC): A Critical Overview of Recent Clinical Trials and Suggested Guidelines for Future Research. J. Clin. Med., 13.","DOI":"10.3390\/jcm13061540"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"111629","DOI":"10.1016\/j.pnpbp.2026.111629","article-title":"A CBD-Rich Hemp Extract Is Superior to CBD Alone in Reducing Relapse to Methamphetamine-Seeking in Rats","volume":"145","author":"Umpierrez","year":"2026","journal-title":"Prog. Neuro-Psychopharmacol. Biol. Psychiatry"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Luz-Veiga, M., Azevedo-Silva, J., and Fernandes, J.C. (2023). Beyond Pain Relief: A Review on Cannabidiol Potential in Medical Therapies. Pharmaceuticals, 16.","DOI":"10.3390\/ph16020155"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Jastrz\u0105b, A., Jarocka-Karpowicz, I., and Skrzydlewska, E. (2022). The Origin and Biomedical Relevance of Cannabigerol. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23147929"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"103692","DOI":"10.1016\/j.redox.2025.103692","article-title":"Cannabinol (CBN) Alleviates Age-Related Cognitive Decline by Improving Synaptic and Mitochondrial Health","volume":"84","author":"Dar","year":"2025","journal-title":"Redox Biol."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Al-Khazaleh, A.K., Zhou, X., Bhuyan, D.J., M\u00fcnch, G.W., Al-Dalabeeh, E.A., Jaye, K., and Chang, D. (2024). The Neurotherapeutic Arsenal in Cannabis Sativa: Insights into Anti-Neuroinflammatory and Neuroprotective Activity and Potential Entourage Effects. Molecules, 29.","DOI":"10.3390\/molecules29020410"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Li, S., Li, W., Malhi, N.K., Huang, J., Li, Q., Zhou, Z., Wang, R., Peng, J., Yin, T., and Wang, H. (2024). Cannabigerol (CBG): A Comprehensive Review of Its Molecular Mechanisms and Therapeutic Potential. Molecules, 29.","DOI":"10.3390\/molecules29225471"},{"key":"ref_23","first-page":"607","article-title":"Review of the Endocannabinoid System","volume":"6","author":"Lu","year":"2021","journal-title":"Biol. Psychiatry Cogn. Neurosci. Neuroimaging"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Rabino, M., Mallia, S., Castiglioni, E., Rovina, D., Pompilio, G., and Gowran, A. (2021). The Endocannabinoid System and Cannabidiol: Past, Present, and Prospective for Cardiovascular Diseases. Pharmaceuticals, 14.","DOI":"10.3390\/ph14090936"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"105226","DOI":"10.1016\/j.neubiorev.2023.105226","article-title":"CB2 Receptor in the CNS: From Immune and Neuronal Modulation to Behavior","volume":"150","author":"Grabon","year":"2023","journal-title":"Neurosci. Biobehav. Rev."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Rezende, B., Alencar, A.K.N., de Bem, G.F., Fontes-Dantas, F.L., and Montes, G.C. (2023). Endocannabinoid System: Chemical Characteristics and Biological Activity. Pharmaceuticals, 16.","DOI":"10.3390\/ph16020148"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1186\/s13041-021-00801-x","article-title":"Cannabinoid Receptors Distribution in Mouse Cortical Plasma Membrane Compartments","volume":"14","author":"Bhatti","year":"2021","journal-title":"Mol. Brain"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"118044","DOI":"10.1016\/j.biopha.2025.118044","article-title":"Cannabinoid Receptor 2 (CB2R) as Potential Target for the Pharmacological Treatment of Neurodegenerative Diseases","volume":"186","author":"Meanti","year":"2025","journal-title":"Biomed. Pharmacother."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"120856","DOI":"10.1016\/j.jep.2025.120856","article-title":"Therapeutic potential of cannabidiol-rich Cannabis sativa to mitigate the severity of inflammation and pain: A pre-clinical study","volume":"357","author":"Singh","year":"2026","journal-title":"J. Ethnopharmacol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"122581","DOI":"10.1016\/j.jchromb.2021.122581","article-title":"Extraction of Medicinal Cannabinoids through Supercritical Carbon Dioxide Technologies: A Review","volume":"1167","author":"Qamar","year":"2021","journal-title":"J. Chromatogr. B"},{"key":"ref_31","unstructured":"European Medicines Agency (2021). Compilation of Terms and Definitions for Cannabis-Derived Medicinal Products, European Medicines Agency."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"116554","DOI":"10.1016\/j.trac.2022.116554","article-title":"Cannabis sativa bioactive compounds and their extraction, separation, purification, and identification technologies: An updated review","volume":"149","author":"Liu","year":"2022","journal-title":"TrAC Trends Anal. Chem."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"e00785","DOI":"10.1016\/j.neurot.2025.e00785","article-title":"Interactions between Cannabis Use and Chronic Pain on Sleep Architecture: Findings from in-Home EEG Recordings","volume":"23","author":"Brown","year":"2026","journal-title":"Neurotherapeutics"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Iftikhar, A., Zafar, U., Ahmed, W., Shabbir, M.A., Sameen, A., Sahar, A., Bhat, Z.F., Kowalczewski, P.\u0141., Jarz\u0119bski, M., and Aadil, R.M. (2021). Applications of Cannabis sativa L. in Food and Its Therapeutic Potential: From a Prohibited Drug to a Nutritional Supplement. Molecules, 26.","DOI":"10.3390\/molecules26247699"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"133207","DOI":"10.1016\/j.colsurfa.2024.133207","article-title":"Cannabis Extract-Loaded Lipid and Chitosan-Coated Lipid Nanoparticles with Antifungal Activity","volume":"685","author":"Menossi","year":"2024","journal-title":"Colloids Surf. A Physicochem. Eng. Asp."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"130676","DOI":"10.1016\/j.colsurfa.2022.130676","article-title":"Prepara\u00e7\u00e3o e Caracteriza\u00e7\u00e3o de Nanopart\u00edculas de Poli(Tio\u00e9ter-\u00c9ster) Carregadas Com Extrato de Cannabis de Amplo Espectro: Avalia\u00e7\u00e3o in Vitro de Sua Efic\u00e1cia Antitumoral","volume":"658","author":"Freire","year":"2023","journal-title":"Colloids Surf. A Physicochem. Eng. Asp."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Silva, E.F.D., Santos, F.A.L.D., Pires, H.M., Bastos, L.M., and Ribeiro, L.N.D.M. (2025). Lipid Nanoparticles Carrying Essential Oils for Multiple Applications as Antimicrobials. Pharmaceutics, 17.","DOI":"10.3390\/pharmaceutics17020178"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Opara, E. (2020). Liposome-Based Delivery of Therapeutic Agents. Controlled Drug Delivery Systems, CRC Press.","DOI":"10.1201\/9780429197833"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"124149","DOI":"10.1016\/j.ijpharm.2024.124149","article-title":"Antitumor Activity of Essential Oils-Based Nanostructured Lipid Carriers on Prostate Cancer Cells","volume":"657","author":"Sommerfeld","year":"2024","journal-title":"Int. J. Pharm."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Ribeiro, L.N.M., Rodrigues da Silva, G.H., Couto, V.M., Castro, S.R., Breitkreitz, M.C., Martinez, C.S., Igart\u00faa, D.E., Prieto, M.J., and de Paula, E. (2020). Functional Hybrid Nanoemulsions for Sumatriptan Intranasal Delivery. Front. Chem., 8.","DOI":"10.3389\/fchem.2020.589503"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"102664","DOI":"10.1016\/j.nano.2023.102664","article-title":"Evaluation of Cannabidiol Nanoparticles and Nanoemulsion Biodistribution in the Central Nervous System after Intrathecal Administration for the Treatment of Pain","volume":"49","author":"Muresan","year":"2023","journal-title":"Nanomed. Nanotechnol. Biol. Med."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"105698","DOI":"10.1016\/j.ejps.2020.105698","article-title":"Mucoadhesive Nanostructured Lipid Carriers as a Cannabidiol Nasal Delivery System for the Treatment of Neuropathic Pain","volume":"159","author":"Matarazzo","year":"2021","journal-title":"Eur. J. Pharm. Sci."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Franz\u00e8, S., Angelo, L., Casiraghi, A., Minghetti, P., and Cilurzo, F. (2022). Design of Liposomal Lidocaine\/Cannabidiol Fixed Combinations for Local Neuropathic Pain Treatment. Pharmaceutics, 14.","DOI":"10.3390\/pharmaceutics14091915"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1186\/s42238-024-00257-5","article-title":"CBD for Pets: Navigating Quality Assurance, Safety Standards, and Marketing Strategies","volume":"7","author":"Rideout","year":"2025","journal-title":"J. Cannabis Res."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Miranda-Cort\u00e9s, A., Mota-Rojas, D., Crosignani-Outeda, N., Casas-Alvarado, A., Mart\u00ednez-Burnes, J., Olmos-Hern\u00e1ndez, A., Mora-Medina, P., Verduzco-Mendoza, A., and Hern\u00e1ndez-\u00c1valos, I. (2023). The Role of Cannabinoids in Pain Modulation in Companion Animals. Front. Vet. Sci., 9.","DOI":"10.3389\/fvets.2022.1050884"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Di Salvo, A., Conti, M.B., and Della Rocca, G. (2023). Pharmacokinetics, Efficacy, and Safety of Cannabidiol in Dogs: An Update of Current Knowledge. Front. Vet. Sci., 10.","DOI":"10.3389\/fvets.2023.1204526"},{"key":"ref_47","first-page":"948","article-title":"Cannabis sativa in Veterinary Medicine: Foundations and Therapeutic Applications","volume":"65","author":"Pinto","year":"2024","journal-title":"Can. Vet. J."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Aragona, F., Tabb\u00ec, M., Gugliandolo, E., Giannetto, C., D\u2019Angelo, F., Fazio, F., and Interlandi, C. (2024). Role of Cannabidiolic Acid or the Combination of Cannabigerol\/Cannabidiol in Pain Modulation and Welfare Improvement in Horses with Chronic Osteoarthritis. Front. Vet. Sci., 11.","DOI":"10.3389\/fvets.2024.1496473"},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Zanelli, G.R., Vieira, G.B.M., Souza, R.V.M., Aguiar, A.J.D.A., and Cassu, R.N. (2024). Perioperative Analgesic and Sedative Effects of Cannabidiol in Cats Undergoing Ovariohysterectomy. Animals, 14.","DOI":"10.3390\/ani14162286"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Brioschi, F.A., Di Cesare, F., Gioeni, D., Rabbogliatti, V., Ferrari, F., D\u2019Urso, E.S., Amari, M., and Ravasio, G. (2020). Oral Transmucosal Cannabidiol Oil Formulation as Part of a Multimodal Analgesic Regimen: Effects on Pain Relief and Quality of Life Improvement in Dogs Affected by Spontaneous Osteoarthritis. Animals, 10.","DOI":"10.3390\/ani10091505"},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Lyons, C., Pinto, K.R., Penney, K., Holmes, L., Salama, A., Alcorn, J., and Chicoine, A. (2025). Efficacy of a 20:1 CBD:THC Cannabis Herbal Extract for Pain and Inflammation in Dogs Following Tibial Plateau Leveling Osteotomy. Front. Vet. Sci., 12.","DOI":"10.3389\/fvets.2025.1676779"},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Talsma, B., Elam, L.H., McGrath, S., Zhou, T., Webb, C.B., and Duerr, F.M. (2024). Evaluation of the Effect of Cannabidiol Administration with and without Nonsteroidal Anti-Inflammatory Drugs in Dogs with Mobility Disorders: A Prospective, Double-Blind, Crossover, Placebo-Controlled Study. Front. Vet. Sci., 11.","DOI":"10.3389\/fvets.2024.1449343"},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Gamble, L.-J., Boesch, J.M., Frye, C.W., Schwark, W.S., Mann, S., Wolfe, L., Brown, H., Berthelsen, E.S., and Wakshlag, J.J. (2018). Pharmacokinetics, Safety, and Clinical Efficacy of Cannabidiol Treatment in Osteoarthritic Dogs. Front. Vet. Sci., 5.","DOI":"10.3389\/fvets.2018.00165"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Shilo-Benjamini, Y., Cern, A., Zilbersheid, D., Hod, A., Lavy, E., Barasch, D., and Barenholz, Y. (2022). A Case Report of Subcutaneously Injected Liposomal Cannabidiol Formulation Used as a Compassion Therapy for Pain Management in a Dog. Front. Vet. Sci., 9.","DOI":"10.3389\/fvets.2022.892306"},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Shilo-Benjamini, Y., Abu Ahmad, W., Barasch, D., Lavy, E., Zilbersheid, D., Barenholz, Y., and Cern, A. (2025). Liposomal-Synthetic-Cannabidiol: Preliminary Translational Evidence of Efficacy, Tolerability and Pharmacokinetics Following Repeated Subcutaneous Injections in Two Goats. Front. Pharmacol., 16.","DOI":"10.3389\/fphar.2025.1689226"},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Shilo-Benjamini, Y., Lavy, E., Yair, N., Milgram, J., Zilbersheid, D., Hod, A., Barasch, D., Abu Ahmad, W., Cern, A., and Barenholz, Y. (2023). Therapeutic Efficacy and Pharmacokinetics of Liposomal-Cannabidiol Injection: A Pilot Clinical Study in Dogs with Naturally-Occurring Osteoarthritis. Front. Vet. Sci., 10.","DOI":"10.3389\/fvets.2023.1224452"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"885","DOI":"10.1124\/pharmrev.122.000600","article-title":"Goods and Bads of the Endocannabinoid System as a Therapeutic Target: Lessons Learned after 30 Years","volume":"75","author":"Maccarrone","year":"2023","journal-title":"Pharmacol. Rev."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Qin, X., Yang, X., Deng, Y., Guo, L., Li, Z., Yang, X., and Hou, C. (2025). Cannabis Derivatives as Ingredients of Functional Foods to Combat the COVID-19 Pandemic. Foods, 14.","DOI":"10.3390\/foods14162830"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"10","DOI":"10.33545\/30810620.2025.v2.i1.A.8","article-title":"Synergistic Effects of Polyherbal Formulations in Phytomedicine: A Review of Traditional and Contemporary Evidence","volume":"2","author":"Kaelen","year":"2025","journal-title":"J. Phytopharm. Phytomed."},{"key":"ref_60","first-page":"229","article-title":"Therapeutic Uses of Cannabis in Indian Traditional Medicine with Attention on Gastrointestinal Disorders","volume":"17","year":"2024","journal-title":"Res. J. Pharm. Technol."},{"key":"ref_61","first-page":"17","article-title":"Regulatory Landscapes for Approval of Naturally-Derived Complex Mixture Drugs","volume":"Volume 32","author":"Sasisekharan","year":"2019","journal-title":"The Science and Regulations of Naturally Derived Complex Drugs"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1007\/s11916-024-01227-5","article-title":"Recent Advances in Management of Neuropathic, Nociceptive, and Chronic Pain: A Narrative Review with Focus on Nanomedicine, Gene Therapy, Stem Cell Therapy, and Newer Therapeutic Options","volume":"28","author":"Kataria","year":"2024","journal-title":"Curr. Pain Headache Rep."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"16","DOI":"10.4103\/jdras.jdras_6_23","article-title":"Pharmacognostical Specification of Cannabis sativa L. (Bhanga) Leaves Processed with Various Ayurveda-Recommended Methods","volume":"9","author":"Tavhare","year":"2024","journal-title":"J. Drug Res. Ayurvedic Sci."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"107452","DOI":"10.1016\/j.yebeh.2020.107452","article-title":"Current Legal Status of Medical Marijuana and Cannabidiol in the United States","volume":"112","author":"Alharbi","year":"2020","journal-title":"Epilepsy Behav."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1080\/09540261.2018.1474730","article-title":"Cannabis and Cannabinoid Drug Development: Evaluating Botanical versus Single Molecule Approaches","volume":"30","author":"ElSohly","year":"2018","journal-title":"Int. Rev. Psychiatry"},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Borowicz-Reutt, K., Czernia, J., and Krawczyk, M. (2024). CBD in the Treatment of Epilepsy. Molecules, 29.","DOI":"10.3390\/molecules29091981"},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Pamplona, F.A., Da Silva, L.R., and Coan, A.C. (2018). Potential Clinical Benefits of CBD-Rich Cannabis Extracts Over Purified CBD in Treatment-Resistant Epilepsy: Observational Data Meta-Analysis. Front. Neurol., 9.","DOI":"10.3389\/fneur.2018.00759"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"106810","DOI":"10.1016\/j.bioorg.2023.106810","article-title":"An Overview on Synthetic and Biological Activities of Cannabidiol (CBD) and Its Derivatives","volume":"140","author":"Wang","year":"2023","journal-title":"Bioorganic Chem."}],"container-title":["Pharmaceutics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4923\/18\/7\/844\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T04:13:41Z","timestamp":1784002421000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4923\/18\/7\/844"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,11]]},"references-count":68,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2026,7]]}},"alternative-id":["pharmaceutics18070844"],"URL":"https:\/\/doi.org\/10.3390\/pharmaceutics18070844","relation":{},"ISSN":["1999-4923"],"issn-type":[{"value":"1999-4923","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,11]]}}}