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Methods and Results: Here, an animal source of PRP, named ApiRegenin and derived from cultivated deer blood, was established. Specific protein and non-protein biomarkers\u2014including nicotinamide, palmitic acid, IGF, and fibronectin\u2014were validated to ensure batch-to-batch quality control. The pharmacological properties of ApiRegenin in cultured cells transfected with DNA encoding HRE and NF-\u03baB reporter constructs were validated, serving as a functional control. In a skin-defective model of db\/db diabetic mice, accelerated wound healing was observed following ApiRegenin treatment. Histological analysis revealed enhancements of re-epithelialization, granulation tissue formation, and collagen deposition. In parallel, the immunofluorescence staining of CD31, \u03b1-SMA, and VEGF was upregulated, indicating the promotion of angiogenesis. Furthermore, ApiRegenin treatment shifted the local immune microenvironment toward an M2-like macrophage phenotype, characterized by the downregulation of iNOS and the contrastive upregulation of Arg-1. At the molecular level, transcriptomic enrichment analysis suggested the prominent involvement of the HIF-1, PI3K-Akt, and TNF signaling pathways. Conclusions: These findings demonstrate that ApiRegenin effectively accelerates diabetic wound healing by promoting angiogenesis and modulating macrophage polarization.<\/jats:p>","DOI":"10.3390\/pharmaceutics18070856","type":"journal-article","created":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T03:06:52Z","timestamp":1784084812000},"page":"856","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["ApiRegenin, an Animal-Derived Platelet-Rich Plasma Extract, Accelerates Wound Healing of Chronic Diabetic Ulcer in Mice"],"prefix":"10.3390","volume":"18","author":[{"given":"Zheng-Qi","family":"Wang","sequence":"first","affiliation":[{"name":"Center for Chinese Medicine, Division of Life Science, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong 999077, China"},{"name":"Shenzhen Key Laboratory of Edible and Medicinal Bioresources, HKUST Shenzhen Research Institute, Shenzhen 518057, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-6456-0779","authenticated-orcid":false,"given":"Minnie Wing-Yi","family":"Mak","sequence":"additional","affiliation":[{"name":"Center for Chinese Medicine, Division of Life Science, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong 999077, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1185-0759","authenticated-orcid":false,"given":"Xiong","family":"Gao","sequence":"additional","affiliation":[{"name":"Center for Chinese Medicine, Division of Life Science, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong 999077, China"},{"name":"Shenzhen Key Laboratory of Edible and Medicinal Bioresources, HKUST Shenzhen Research Institute, Shenzhen 518057, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Tong","family":"Ye","sequence":"additional","affiliation":[{"name":"Center for Chinese Medicine, Division of Life Science, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong 999077, China"},{"name":"Shenzhen Key Laboratory of Edible and Medicinal Bioresources, HKUST Shenzhen Research Institute, Shenzhen 518057, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina Lok-Pan","family":"Yik","sequence":"additional","affiliation":[{"name":"Center for Chinese Medicine, Division of Life Science, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong 999077, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tina Ting-Xia","family":"Dong","sequence":"additional","affiliation":[{"name":"Center for Chinese Medicine, Division of Life Science, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong 999077, China"},{"name":"Shenzhen Key Laboratory of Edible and Medicinal Bioresources, HKUST Shenzhen Research Institute, Shenzhen 518057, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4808-1674","authenticated-orcid":false,"given":"Karl Wah-Keung","family":"Tsim","sequence":"additional","affiliation":[{"name":"Center for Chinese Medicine, Division of Life Science, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong 999077, China"},{"name":"Shenzhen Key Laboratory of Edible and Medicinal Bioresources, HKUST Shenzhen Research Institute, Shenzhen 518057, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1038\/nrendo.2017.151","article-title":"Global aetiology and epidemiology of type 2 diabetes mellitus and its complications","volume":"14","author":"Zheng","year":"2018","journal-title":"Nat. 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