{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T19:06:13Z","timestamp":1789499173952,"version":"build-2803163510"},"reference-count":147,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004252","name":"Qatar University","doi-asserted-by":"publisher","award":["QUCG-CPH-26\/27-913"],"award-info":[{"award-number":["QUCG-CPH-26\/27-913"]}],"id":[{"id":"10.13039\/501100004252","id-type":"DOI","asserted-by":"publisher"}]},{"award":["QUCG-CPH-26\/27-913"],"award-info":[{"award-number":["QUCG-CPH-26\/27-913"]}],"id":[{"id":"https:\/\/ror.org\/00yhnba62","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmaceutics"],"abstract":"<jats:p>Biomaterials intended for wound healing are expected to reduce microbial load while supporting tissue repair in complex wound environments. Plant-mediated synthesis of silver nanoparticles (AgNPs) has attracted interest because plant extracts can provide phytochemicals that mediate nanoparticle reduction, capping, and stabilization. This review critically evaluates recent studies on plant-mediated AgNPs for wound-healing applications, focusing on botanical and extraction variables, synthesis conditions, physicochemical properties, formulation strategies, administered exposure, safety, and biological outcomes. Across the 34 included publications, 14 directly assessed a process-to-property relationship and only two assessed a process-to-performance relationship; none directly assessed a property-to-performance relationship or established a complete process-to-property-to-performance chain. The reviewed studies suggest that plant-mediated AgNPs may reduce microbial burden, modulate inflammation, mitigate oxidative stress, and promote cell migration, re-epithelialization, collagen remodeling, and angiogenesis. However, batch-to-batch variability, incomplete reporting of experimental details, over-reliance on ultraviolet\u2013visible (UV\u2013Vis) spectroscopy for optimization, inadequate evaluation of formulation stability, inconsistent dose reporting, and limited molecular validation of proposed mechanisms remain major factors limiting translation. By evaluating plant-mediated AgNP-based wound-healing systems through a process\u2013property\u2013performance framework, this review summarizes recent advances and highlights key considerations for improving reproducibility, comparability, and translational relevance.<\/jats:p>","DOI":"10.3390\/pharmaceutics18091161","type":"journal-article","created":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T18:23:53Z","timestamp":1789496633000},"page":"1161","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Plant-Mediated Silver Nanoparticle Formulations for Wound Healing: A Process\u2013Property\u2013Performance Perspective"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3764-9730","authenticated-orcid":false,"given":"Mohammad Moein","family":"Sadeghi","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Sciences, College of Pharmacy, QU Health, Qatar University, Doha P.O. Box 2713, Qatar"},{"name":"School of Pharmacy & Biomolecular Sciences, Royal College of Surgeons in Ireland (RCSI), D02 VN51 Dublin, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Souha","family":"Idoudi","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, College of Pharmacy, QU Health, Qatar University, Doha P.O. Box 2713, Qatar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helena M.","family":"Kelly","sequence":"additional","affiliation":[{"name":"School of Pharmacy & Biomolecular Sciences, Royal College of Surgeons in Ireland (RCSI), D02 VN51 Dublin, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8496-1882","authenticated-orcid":false,"given":"Nashiru","family":"Billa","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, College of Pharmacy, QU Health, Qatar University, Doha P.O. Box 2713, Qatar"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3861","DOI":"10.1007\/s00018-016-2268-0","article-title":"Transition from inflammation to proliferation: A critical step during wound healing","volume":"73","author":"Li","year":"2016","journal-title":"Cell. Mol. Life Sci."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Redmond, M.C., Gethin, G., and Finn, D.P. (2025). A Review of Chronic Wounds and Their Impact on Negative Affect, Cognition, and Quality of Life. Int. Wound J., 22.","DOI":"10.1111\/iwj.70748"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1177\/21621918251359554","article-title":"Human Wound and Its Burden: Updated 2025 Compendium of Estimates","volume":"14","author":"Sen","year":"2025","journal-title":"Adv. Wound Care"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Pastar, I., Balukoff, N.C., Marjanovic, J., Chen, V.Y., Stone, R.C., and Tomic-Canic, M. (2023). Molecular Pathophysiology of Chronic Wounds: Current State and Future Directions. Cold Spring Harb. Perspect. Biol., 15.","DOI":"10.1101\/cshperspect.a041243"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1016\/j.jconrel.2021.07.017","article-title":"Nanomaterials applied in wound healing: Mechanisms, limitations and perspectives","volume":"337","author":"Wang","year":"2021","journal-title":"J. Control. Release"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Kumar, R., Igwegbe, C.A., and Khandel, S.K. (2024). Nanotherapeutic and Nano-Bio Interface for Regeneration and Healing. Biomedicines, 12.","DOI":"10.3390\/biomedicines12122927"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Krishnan, P.D., Banas, D., Durai, R.D., Kabanov, D., Hosnedlova, B., Kepinska, M., Fernandez, C., Ruttkay-Nedecky, B., Nguyen, H.V., and Farid, A. (2020). Silver Nanomaterials for Wound Dressing Applications. Pharmaceutics, 12.","DOI":"10.3390\/pharmaceutics12090821"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Kaya, M., Akda\u015f\u00e7i, E., Eker, F., Bechelany, M., and Karav, S. (2025). Recent Advances of Silver Nanoparticles in Wound Healing: Evaluation of In Vivo and In Vitro Studies. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26209889"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Dahri, M., Rezaeian, M., Sadeghzadeh, H., Beheshtizadeh, N., Sadeghi, M.M., Zakerhamidi, D., Faraji, S.N., Pakdel, H., Dahri, B., and Maleki, R. (2025). Nanomaterial-driven macrophage polarization: Emerging strategies for immunomodulation and regenerative medicine. Biomed. Pharmacother., 190.","DOI":"10.1016\/j.biopha.2025.118360"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Ferdous, Z., and Nemmar, A. (2020). Health Impact of Silver Nanoparticles: A Review of the Biodistribution and Toxicity Following Various Routes of Exposure. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21072375"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Zhang, X.F., Liu, Z.G., Shen, W., and Gurunathan, S. (2016). Silver Nanoparticles: Synthesis, Characterization, Properties, Applications, and Therapeutic Approaches. Int. J. Mol. Sci., 17.","DOI":"10.3390\/ijms17091534"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Eker, F., Akda\u015f\u00e7i, E., Duman, H., Bechelany, M., and Karav, S. (2025). Green synthesis of silver nanoparticles using plant extracts: A comprehensive review of physicochemical properties and multifunctional applications. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26136222"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Dhir, R., Chauhan, S., Subham, P., Kumar, S., Sharma, P., Shidiki, A., and Kumar, G. (2024). Plant-mediated synthesis of silver nanoparticles: Unlocking their pharmacological potential\u2013a comprehensive review. Front. Bioeng. Biotechnol., 11.","DOI":"10.3389\/fbioe.2023.1324805"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"7547","DOI":"10.1021\/acs.iecr.2c00285","article-title":"Biomimetic metallic nanostructures for biomedical applications, catalysis, and beyond","volume":"61","author":"Yaraki","year":"2022","journal-title":"Ind. Eng. Chem. Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2804","DOI":"10.1039\/D0RA09941D","article-title":"Green synthesis of silver nanoparticles using plant extracts and their antimicrobial activities: A review of recent literature","volume":"11","author":"Vanlalveni","year":"2021","journal-title":"RSC Adv."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Thatyana, M., Dube, N.P., Kemboi, D., Manicum, A.E., Mokgalaka-Fleischmann, N.S., and Tembu, J.V. (2023). Advances in Phytonanotechnology: A Plant-Mediated Green Synthesis of Metal Nanoparticles Using Phyllanthus Plant Extracts and Their Antimicrobial and Anticancer Applications. Nanomaterials, 13.","DOI":"10.3390\/nano13192616"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Simon, S., Sibuyi, N.R.S., Fadaka, A.O., Meyer, S., Josephs, J., Onani, M.O., Meyer, M., and Madiehe, A.M. (2022). Biomedical applications of plant extract-synthesized silver nanoparticles. Biomedicines, 10.","DOI":"10.3390\/biomedicines10112792"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1049\/iet-nbt.2015.0084","article-title":"Evaluation of the wound healing efficacy of chemical and phytogenic silver nanoparticles","volume":"10","author":"Pothireddy","year":"2016","journal-title":"IET Nanobiotechnol."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Ahmad, B., Chang, L., Yin, C., Wu, Z., Tong, A., Tong, C., and Liu, B. (2024). Assessing Phytogenic and Chemogenic Silver Nanoparticles for Antibacterial Activity and Expedited Wound Recuperation. Nanomaterials, 14.","DOI":"10.3390\/nano14030237"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2625","DOI":"10.1007\/s13204-021-02135-5","article-title":"A review of the phytochemical mediated synthesis of AgNP (silver nanoparticle): The wonder particle of the past decade","volume":"11","author":"Shaikh","year":"2021","journal-title":"Appl. Nanosci."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Noga, M., Milan, J., Frydrych, A., and Jurowski, K. (2023). Toxicological Aspects, Safety Assessment, and Green Toxicology of Silver Nanoparticles (AgNPs)\u2014Critical Review: State of the Art. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24065133"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Nie, P., Zhao, Y., and Xu, H. (2023). Synthesis, applications, toxicity and toxicity mechanisms of silver nanoparticles: A review. Ecotoxicol. Environ. Saf., 253.","DOI":"10.1016\/j.ecoenv.2023.114636"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"He, J., Ma, Y., Niu, X., Pei, J., Yan, R., Xu, F., Ma, J., Ma, X., Jia, S., and Ma, W. (2024). Silver nanoparticles induce endothelial cytotoxicity through ROS-mediated mitochondria-lysosome damage and autophagy perturbation: The protective role of N-acetylcysteine. Toxicology, 502.","DOI":"10.1016\/j.tox.2024.153734"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Oselusi, S.O., Sibuyi, N.R., Meyer, M., and Madiehe, A.M. (2025). Anti-inflammatory, cytotoxic, and potential wound healing effects of phytofabricated Ehretia rigida leaf aqueous extract-synthesized silver nanoparticles. Sci. Rep., 15.","DOI":"10.1038\/s41598-025-24111-7"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1038\/s41565-018-0246-4","article-title":"Minimum information reporting in bio-nano experimental literature","volume":"13","author":"Faria","year":"2018","journal-title":"Nat. Nanotechnol."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Ansari, M., Ahmed, S., Abbasi, A., Khan, M.T., Subhan, M., Bukhari, N.A., Hatamleh, A.A., and Abdelsalam, N.R. (2023). Plant mediated fabrication of silver nanoparticles, process optimization, and impact on tomato plant. Sci. Rep., 13.","DOI":"10.1038\/s41598-023-45038-x"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Miranda, A., Akpobolokemi, T., Chung, E., Ren, G., and Raimi-Abraham, B.T. (2022). pH Alteration in Plant-Mediated Green Synthesis and Its Resultant Impact on Antimicrobial Properties of Silver Nanoparticles (AgNPs). Antibiotics, 11.","DOI":"10.3390\/antibiotics11111592"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Velgosova, O., Ma\u010d\u00e1k, L., Lisnichuk, M., and Varga, P. (2025). Influence of pH and Temperature on the Synthesis and Stability of Biologically Synthesized AgNPs. Appl. Nano, 6.","DOI":"10.3390\/applnano6040022"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1093\/jbcr\/iraa205","article-title":"Incorporation of Silver Nanoparticles in Hydrogel Matrices for Controlling Wound Infection","volume":"42","author":"Pangli","year":"2021","journal-title":"J. Burn Care Res."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Nicolae-Maranciuc, A., and Chicea, D. (2025). Polymeric Systems as Hydrogels and Membranes Containing Silver Nanoparticles for Biomedical and Food Applications: Recent Approaches and Perspectives. Gels, 11.","DOI":"10.3390\/gels11090699"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1111\/iep.12346","article-title":"Experimental models and methods for cutaneous wound healing assessment","volume":"101","author":"Andrade","year":"2020","journal-title":"Int. J. Exp. Pathol."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Hofmann, E., Fink, J., Pignet, A.-L., Schwarz, A., Schellnegger, M., Nischwitz, S.P., Holzer-Geissler, J.C., Kamolz, L.-P., and Kotzbeck, P. (2023). Human in vitro skin models for wound healing and wound healing disorders. Biomedicines, 11.","DOI":"10.3390\/biomedicines11041056"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Velgosova, O., Dolinsk\u00e1, S., Podolsk\u00e1, H., Ma\u010d\u00e1k, L., and \u010ci\u017em\u00e1rov\u00e1, E. (2024). Impact of plant extract phytochemicals on the synthesis of silver nanoparticles. Materials, 17.","DOI":"10.3390\/ma17102252"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"14191","DOI":"10.1007\/s11356-024-32180-w","article-title":"Valorization of plant by-products in the biosynthesis of silver nanoparticles with antimicrobial and catalytic properties","volume":"31","author":"Rocha","year":"2024","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Alami, M.M., Guo, S., Mei, Z., Yang, G., and Wang, X. (2024). Environmental factors on secondary metabolism in medicinal plants: Exploring accelerating factors. Med. Plant Biol., 3.","DOI":"10.48130\/mpb-0024-0016"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Lima, A.K.O., Vieira, \u00cd.R.S., Souza, L.M.d.S., Flor\u00eancio, I., Silva, I.G.M.d., Tavares Junior, A.G., Machado, Y.A.A., Santos, L.C.d., Taube, P.S., and Nakazato, G. (2025). Green synthesis of silver nanoparticles using Paullinia cupana Kunth leaf extract collected in different seasons: Biological studies and catalytic properties. Pharmaceutics, 17.","DOI":"10.3390\/pharmaceutics17030356"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Martins, R., Barbosa, A., Advinha, B., Sales, H., Pontes, R., and Nunes, J. (2023). Green extraction techniques of bioactive compounds: A state-of-the-art review. Processes, 11.","DOI":"10.3390\/pr11082255"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"9369","DOI":"10.1021\/jf501574q","article-title":"Phenolic compounds in Rosaceae fruit and nut crops","volume":"62","author":"Ogah","year":"2014","journal-title":"J. Agric. Food Chem."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Gladikosti\u0107, N., Ikoni\u0107, B., Tesli\u0107, N., Zekovi\u0107, Z., Bo\u017eovi\u0107, D., Putnik, P., Bursa\u0107 Kova\u010devi\u0107, D., and Pavli\u0107, B. (2023). Essential Oils from Apiaceae, Asteraceae, Cupressaceae and Lamiaceae Families Grown in Serbia: Comparative Chemical Profiling with In Vitro Antioxidant Activity. Plants, 12.","DOI":"10.3390\/plants12040745"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"S\u00e1nchez, M., Gonz\u00e1lez-Burgos, E., Iglesias, I., and G\u00f3mez-Serranillos, M.P. (2020). Pharmacological update properties of Aloe vera and its major active constituents. Molecules, 25.","DOI":"10.3390\/molecules25061324"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Kumari, A., Raina, N., Wahi, A., Goh, K.W., Sharma, P., Nagpal, R., Jain, A., Ming, L.C., and Gupta, M. (2022). Wound-healing effects of curcumin and its nanoformulations: A comprehensive review. Pharmaceutics, 14.","DOI":"10.3390\/pharmaceutics14112288"},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Alzohairy, M.A. (2016). Therapeutics role of Azadirachta indica (Neem) and their active constituents in diseases prevention and treatment. Evid.-Based Complement. Altern. Med., 2016.","DOI":"10.1155\/2016\/7382506"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"S4081","DOI":"10.4103\/jpbs.jpbs_1439_24","article-title":"Anti-Inflammatory Activity and Free Radical Scavenging Activity of Tridax Procumbens Leaves-Based Chitosan Gel","volume":"16","author":"Nandita","year":"2024","journal-title":"J. Pharm. Bioallied Sci."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Boudou, F., Belakredar, A., Keziz, A., Alsaeedi, H., Cornu, D., Bechelany, M., and Barhoum, A. (2025). Camellia sinensis phytochemical profiling, drug-likeness, and antibacterial activity against gram-positive and gram-negative bacteria: In vitro and in silico insights. Front. Chem., 13.","DOI":"10.3389\/fchem.2025.1555574"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Dubey, S., and Dixit, A.K. (2023). Preclinical evidence of polyherbal formulations on wound healing: A systematic review on research trends and perspectives. J. Ayurveda Integr. Med., 14.","DOI":"10.1016\/j.jaim.2023.100688"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Yu, Z., Xie, C., Liu, M., Chen, Y., Liu, H., Zhang, J., Wang, Q., Lei, P., Yan, F., and Long, Y. (2026). Eco-Synthesized silver nanoparticles from Pyrrosia petiolosa: Lymphocyte-sparing activity and targeted full-Thickness traumatic wound healing via motor protein modulation. Green Chem. Lett. Rev., 19.","DOI":"10.1080\/17518253.2026.2649033"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Tahamtan, M.S., Ansari, F., Moharrami Kasmaie, F., Zareshahrabadi, Z., Amani, A.M., Salehi, M., Amiri, S., Ghasemian, S., Askari Jahromi, M., and Vaez, A. (2026). Integration of green synthesized silver nanoparticles in electrospun PVA-alginate and resveratrol-loaded ZIF-8 into alginate lyophilized hydrogel as a dual-layered biomimetic skin construct. Int. J. Biol. Macromol., 338.","DOI":"10.1016\/j.ijbiomac.2025.149681"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"2230","DOI":"10.1007\/s44174-025-00366-6","article-title":"Microwave-Assisted Green Synthesis of Silver Nanoparticles Using Euphorbia hirta and Ocimum americanum: Optimization, Characterization, and Biomedical Applications","volume":"4","author":"Karthikeyan","year":"2026","journal-title":"Biomed. Mater. Devices"},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Gupta, T., Badhwar, R., and Singh, R. (2026). Green synthesis of Carbopol-silver nanoparticles using Hibiscus rosasinensis and Cymbopogon citratus: A synergistic in vitro and in vivo approach for accelerated wound healing in Swiss albino mice. Biocatal. Agric. Biotechnol., 73.","DOI":"10.1016\/j.bcab.2026.103975"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"G\u00fcneri, G., and Keskin, M. (2026). An In Vitro Study on the Efficacy of Green Synthesized Silver Nanoparticles on Surgical Site Infections and Healings. Biomedicines, 14.","DOI":"10.3390\/biomedicines14030634"},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Dehghan, H., Mohammadparast-Tabas, P., Ghasempour, A., Soleimanmanesh, Y., Jafari Bidhendi, M., Nabavi Sales, F.S., Gholami, A., Zare-Bidaki, M., and Mortazavi-Derazkola, S. (2026). Eco-inspired synthesis of silver nanoparticles from Prunus avium stems: Mechanistic insights into multifunctional biomedical activities. Mater. Today Commun., 51.","DOI":"10.1016\/j.mtcomm.2026.114717"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"6514","DOI":"10.1007\/s12011-026-05130-5","article-title":"In Vivo Wound Healing Activity Using Pomegranate Peel Derived\u2014Silver Nanoparticle Hydrogel Pre-Exposed with 660 nm Red Light","volume":"204","author":"Ahmed","year":"2026","journal-title":"Biol. Trace Elem. Res."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Wang, X., Huang, J., Zhao, J., Peng, L., Qi, L., Wu, T., He, J., Gao, Y., and Zhou, Y. (2025). Dual stimuli-responsive and self-healing hyaluronic acid-based nanocomposite hydrogels for promoting wound healing of methicillin-resistant Staphylococcus aureus infection. Int. J. Biol. Macromol., 320.","DOI":"10.1016\/j.ijbiomac.2025.145829"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1007\/s12011-025-04666-2","article-title":"Advanced Bioinspired Silver Nanoparticles Integrated into Polyherbal Gel for Enhanced Diabetic Foot Ulcer Regeneration","volume":"204","author":"Shelar","year":"2026","journal-title":"Biol. Trace Elem. Res."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Sharma, S., Bose, A., Biswas, S., Sen, S., and Roy, I. (2025). Cyperus rotundus mediated green synthesis of silver nanoparticles for antibacterial wound dressing applications. Sci. Rep., 15.","DOI":"10.1038\/s41598-025-03555-x"},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Palanisamy, C.P., Poompradub, S., Sansanaphongpricha, K., Jayaraman, S., Subramani, K., and Sonsudin, F. (2025). Green synthesis of Nigella sativa-mediated silver nanoparticles for enhanced antibacterial activity and wound healing: Mechanistic insights and biomedical applications. Environ. Nanotechnol. Monit. Manag., 24.","DOI":"10.1016\/j.enmm.2025.101085"},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Lv, S., Liu, Y., Liu, C., Yin, J., An, D., Deng, S., and Liu, G. (2025). Green construction of Dendrobium officinale extracts-silver nanocomposites for skin wound healing. Ind. Crops Prod., 224.","DOI":"10.1016\/j.indcrop.2024.120384"},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Guzzatti, M.F.M., de Moura, A.B., Venturini, L.M., de Roch Casagrande, L., Lima, I.R., da Costa, C., de Pieri, E., Tietbohl, L.T.W., Feuser, P.E., and Machado-de-\u00c1vila, R.A. (2025). Biosynthesized Silver Nanoparticles Modulate Inflammation in a Palatine Wound Model. Clin. Exp. Dent. Res., 11.","DOI":"10.1002\/cre2.70213"},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Govindarasu, M., Vaiyapuri, M., and Thiruvengadam, M. (2025). Green synthesis of chitosan\u2013silver nanoparticles using Lannea coromandelica: A synergistic in vitro and in vivo approach for accelerated wound healing in BALB\/c mice. Biocatal. Agric. Biotechnol., 70.","DOI":"10.1016\/j.bcab.2025.103876"},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Erol, I., Hazman, \u00d6., Khamidov, G., and B\u00fcy\u00fckben, A. (2025). Enhancement of thermal and biological properties of chitosan by a styrenic polymer and biosynthesized silver nanoparticles. Mater. Today Commun., 44.","DOI":"10.1016\/j.mtcomm.2025.112137"},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Chang, L., Ahmad, B., Tong, A., Wu, Z., Xu, P., Gao, Z., Yin, C., Luan, M., Tong, C., and Liu, B. (2025). Green synthesis of silver nanoparticles using Fructus Aurantii-loaded Citrus pectin hydrogel for accelerated healing of MRSA-infected diabetic wounds. Int. J. Biol. Macromol., 315.","DOI":"10.1016\/j.ijbiomac.2025.144222"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1143","DOI":"10.1007\/s13369-025-09988-w","article-title":"Cassia sericea Leaf Extract for Eco-Friendly Synthesis of Silver and Gold Nanoparticles with Boosted Wound Healing and Antimicrobial Potential","volume":"51","author":"Alsareii","year":"2026","journal-title":"Arab. J. Sci. Eng."},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Verma, P., Dixit, J., Singh, C., Singh, A.N., Singh, A., Tiwari, K.N., Muthu, M.S., Nath, G., and Mishra, S.K. (2024). Preparation of hydrogel from the hydroalcoholic root extract of Premna integrifolia L. and its mediated green synthesis of silver nanoparticles for wound healing efficacy. Mater. Today Commun., 41.","DOI":"10.1016\/j.mtcomm.2024.110228"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"12146","DOI":"10.1021\/acsomega.3c10489","article-title":"Evaluation of Plant-Based Silver Nanoparticles for Antioxidant Activity and Promising Wound-Healing Applications","volume":"9","author":"Qubtia","year":"2024","journal-title":"ACS Omega"},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Murugapandi, M., Elanchezhiyan, S.S., Oh, T.H., Ramasundaram, S., and Muniyappan, N. (2024). An evaluation of the pharmacological responses of metal nanoparticles derived from aqueous extract of Caralluma adscendens R. Brown var. bicolor. J. Mol. Liq., 401.","DOI":"10.1016\/j.molliq.2024.124618"},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Huang, T.Y., Lin, J.Y., and Su, W.T. (2024). Coaxial nanofibers encapsulated with Ampelopsis brevipedunculata extract and green synthesized AgNPs for wound repair. Colloids Surf. B Biointerfaces, 235.","DOI":"10.1016\/j.colsurfb.2024.113771"},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Ahmad, M.Z., Saeed, A.M., Elnoubi, O.A.E., Alasiri, A.S., Abdel-Wahab, B.A., Alqahtani, A.A., Pathak, K., Saikia, R., Kakoti, B.B., and Das, A. (2024). Chitosan-based topical formulation integrated with green-synthesized silver nanoparticles utilizing Camellia sinensis leaf extracts: A promising approach for managing infected wounds. Int. J. Biol. Macromol., 257.","DOI":"10.1016\/j.ijbiomac.2023.128573"},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Zare-Bidaki, M., Ghasempour, A., Mohammadparast-Tabas, P., Ghoreishi, S.M., Alamzadeh, E., Javanshir, R., Le, B.N., Barakchi, M., Fattahi, M., and Mortazavi-Derazkola, S. (2023). Enhanced in vivo wound healing efficacy and excellent antibacterial, antifungal, antioxidant and anticancer activities via AgNPs@PCS. Arab. J. Chem., 16.","DOI":"10.1016\/j.arabjc.2023.105194"},{"key":"ref_69","doi-asserted-by":"crossref","unstructured":"Singh, K., Yadav, V.B., Yadav, U., Nath, G., Srivastava, A., Zamboni, P., Kerkar, P., Saxena, P.S., and Singh, A.V. (2023). Evaluation of biogenic nanosilver-acticoat for wound healing: A tri-modal in silico, in vitro and in vivo study. Colloids Surf. A-Physicochem. Eng. Asp., 670.","DOI":"10.1016\/j.colsurfa.2023.131575"},{"key":"ref_70","doi-asserted-by":"crossref","unstructured":"Kodasi, B., Kamble, R.R., Shettar, A.K., Hoskeri, J.H., Keri, R.S., Metre, T.V., Bheemayya, L., Nadoni, V.B., and Nayak, M.R. (2023). Novel jointured green synthesis of chitosan-silver nanocomposite: An approach towards reduction of nitroarenes, anti-proliferative, wound healing and antioxidant applications. Int. J. Biol. Macromol., 246.","DOI":"10.1016\/j.ijbiomac.2023.125578"},{"key":"ref_71","doi-asserted-by":"crossref","unstructured":"Bharali, A., Sarma, H., Biswas, N., Kalita, J.M., Das, B., Sahu, B.P., Prasad, S.K., and Laloo, D. (2023). Green synthesis of silver nanoparticles using hydroalcoholic root extract of Potentilla fulgens and evaluation of its cutaneous wound healing potential. Mater. Today Commun., 35.","DOI":"10.1016\/j.mtcomm.2023.106050"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"1881","DOI":"10.1007\/s13346-022-01121-w","article-title":"Synthesis and evaluation of wound healing properties of hydro-diab hydrogel loaded with green-synthetized AGNPS: In vitro and in ex vivo studies","volume":"12","author":"Ruffo","year":"2022","journal-title":"Drug Deliv. Transl. Res."},{"key":"ref_73","doi-asserted-by":"crossref","unstructured":"Alsareii, S.A., Manaa Alamri, A., AlAsmari, M.Y., Bawahab, M.A., Mahnashi, M.H., Shaikh, I.A., Shettar, A.K., Hoskeri, J.H., and Kumbar, V. (2022). Synthesis and Characterization of Silver Nanoparticles from Rhizophora apiculata and Studies on Their Wound Healing, Antioxidant, Anti-Inflammatory, and Cytotoxic Activity. Molecules, 27.","DOI":"10.3390\/molecules27196306"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"3633","DOI":"10.1016\/j.sjbs.2021.05.007","article-title":"Green synthesis of silver nanoparticles from aqueous extract of Scutellaria barbata and coating on the cotton fabric for antimicrobial applications and wound healing activity in fibroblast cells (L929)","volume":"28","author":"Veeraraghavan","year":"2021","journal-title":"Saudi J. Biol. Sci."},{"key":"ref_75","doi-asserted-by":"crossref","unstructured":"Shanmugapriya, K., Palanisamy, S., Boomi, P., Subaskumar, R., Ravikumar, S., and Thayumanavan, T. (2021). An eco-friendly Gnaphalium polycaulon mediated silver nanoparticles: Synthesis, characterization, antimicrobial, wound healing and drug release studies. J. Drug Deliv. Sci. Technol., 61.","DOI":"10.1016\/j.jddst.2020.102202"},{"key":"ref_76","doi-asserted-by":"crossref","unstructured":"Shahabadi, N., Zendehcheshm, S., Khademi, F., Rashidi, K., Chehri, K., and Dehpahni, M.F. (2021). Green synthesis of Chloroxine-conjugated silver nanoflowers: Promising antimicrobial activity and in vivo cutaneous wound healing effects. J. Environ. Chem. Eng., 9.","DOI":"10.1016\/j.jece.2021.105215"},{"key":"ref_77","doi-asserted-by":"crossref","unstructured":"Fatima, F., Aldawsari, M.F., Ahmed, M.M., Anwer, M.K., Naz, M., Ansari, M.J., Hamad, A.M., Zafar, A., and Jafar, M. (2021). Green Synthesized Silver Nanoparticles Using Tridax procumbens for Topical Application: Excision Wound Model and Histopathological Studies. Pharmaceutics, 13.","DOI":"10.3390\/pharmaceutics13111754"},{"key":"ref_78","doi-asserted-by":"crossref","unstructured":"Chinnasamy, G., Chandrasekharan, S., Koh, T.W., and Bhatnagar, S. (2021). Synthesis, Characterization, Antibacterial and Wound Healing Efficacy of Silver Nanoparticles From Azadirachta indica. Front. Microbiol., 12.","DOI":"10.3389\/fmicb.2021.611560"},{"key":"ref_79","first-page":"58","article-title":"Green synthesis of silver nanoparticles from different parts of the plant Macrotyloma uniflorum","volume":"3","author":"Mutahir","year":"2015","journal-title":"Octa J. Biosci."},{"key":"ref_80","doi-asserted-by":"crossref","unstructured":"Lima, A.K.O., Souza, L.M.d.S., Reis, G.F., Junior, A.G.T., Ara\u00fajo, V.H.S., Santos, L.C.d., Silva, V.R.P.d., Chorilli, M., Braga, H.d.C., and Tada, D.B. (2024). Synthesis of silver nanoparticles using extracts from different parts of the Paullinia cupana Kunth plant: Characterization and in vitro antimicrobial activity. Pharmaceuticals, 17.","DOI":"10.3390\/ph17070869"},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"3858","DOI":"10.1039\/D4RA07519F","article-title":"A review on green synthesis of silver nanoparticles (SNPs) using plant extracts: A multifaceted approach in photocatalysis, environmental remediation, and biomedicine","volume":"15","author":"Shahzadi","year":"2025","journal-title":"RSC Adv."},{"key":"ref_82","doi-asserted-by":"crossref","unstructured":"Rodr\u00edguez-F\u00e9lix, F., Graciano-Verdugo, A.Z., Moreno-V\u00e1squez, M.J., Lagarda-D\u00edaz, I., Barreras-Urbina, C.G., Armenta-Villegas, L., Olgu\u00edn-Moreno, A., and Tapia-Hern\u00e1ndez, J.A. (2022). Trends in sustainable green synthesis of silver nanoparticles using agri-food waste extracts and their applications in health. J. Nanomater., 2022.","DOI":"10.1155\/2022\/8874003"},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3897\/pharmacia.72.e168925","article-title":"Effect of solvent polarity on the extraction of bioactive compounds from Heracleum persicum fruits","volume":"72","author":"Tsaturyan","year":"2025","journal-title":"Pharmacia"},{"key":"ref_84","doi-asserted-by":"crossref","unstructured":"Kim, S.M., Choi, H.J., Lim, J.A., Woo, M.A., Chang, H.J., Lee, N., and Lim, M.C. (2023). Biosynthesis of Silver Nanoparticles from Duchesnea indica Extracts Using Different Solvents and Their Antibacterial Activity. Microorganisms, 11.","DOI":"10.3390\/microorganisms11061539"},{"key":"ref_85","doi-asserted-by":"crossref","unstructured":"Deshi, J.J., Barminas, J.T., Onwuka, J.C., Dass, P.M., Maitera, O.N., and Muazu, I. (2016). Antimicrobial efficacy of biosynthesized silver nanoparticles from different solvent extracts of Waltheria americana root. J. Anal. Sci. Technol., 7.","DOI":"10.1186\/s40543-016-0104-7"},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"90","DOI":"10.31925\/farmacia.2021.1.12","article-title":"Biogenic synthesis of nanosilver particles from root extract of Morus alba L. and their biological activities","volume":"69","author":"Minhas","year":"2021","journal-title":"Farmacia"},{"key":"ref_87","doi-asserted-by":"crossref","unstructured":"Balu, S.K., Andra, S., Damiri, F., Sivaramalingam, A., Sudandaradoss, M.V., Kumarasamy, K., Bhakthavachalam, K., Ali, F., Kundu, M.K., and Rahman, M.H. (2022). Size-dependent antibacterial, antidiabetic, and toxicity of silver nanoparticles synthesized using solvent extraction of Rosa indica L. Petals. Pharmaceuticals, 15.","DOI":"10.3390\/ph15060689"},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"155","DOI":"10.2174\/1874070701913010155","article-title":"Water an eco-friendly crossroad in green extraction: An overview","volume":"13","author":"Mihaylova","year":"2019","journal-title":"Open Biotechnol. J."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1021\/es403969x","article-title":"Effects of chloride and ionic strength on physical morphology, dissolution, and bacterial toxicity of silver nanoparticles","volume":"48","author":"Chambers","year":"2014","journal-title":"Environ. Sci. Technol."},{"key":"ref_90","doi-asserted-by":"crossref","unstructured":"Byrne, F., Jin, S., Paggiola, G., Petchey, T., Clark, J., Farmer, T., Hunt, A., McElroy, C., and Sherwood, J. (2016). Tools and techniques for solvent selection: Green solvent selection guides. Sustain. Chem. Process., 4.","DOI":"10.1186\/s40508-016-0051-z"},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1021\/ar1001457","article-title":"Microwave-assisted green synthesis of silver nanostructures","volume":"44","author":"Nadagouda","year":"2011","journal-title":"Acc. Chem. Res."},{"key":"ref_92","doi-asserted-by":"crossref","unstructured":"Rashed, M.N., Abdelrady, E., and Ghabrial, T.M. (2025). Microwave assisted green synthesis of silver nanoparticles using Trigonella hamosa L. plant extract for the photodegradation of some water pollutants. Sci. Rep., 15.","DOI":"10.1038\/s41598-025-21112-4"},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"95","DOI":"10.19261\/cjm.2017.461","article-title":"Silver nanoparticles synthesized using Lantana camara flower extract by reflux, microwave and ultrasound methods","volume":"13","author":"Fatimah","year":"2018","journal-title":"Chem. J. Mold."},{"key":"ref_94","doi-asserted-by":"crossref","unstructured":"Liaqat, N., Jahan, N., Khalil Ur, R., Anwar, T., and Qureshi, H. (2022). Green synthesized silver nanoparticles: Optimization, characterization, antimicrobial activity, and cytotoxicity study by hemolysis assay. Front. Chem., 10.","DOI":"10.3389\/fchem.2022.952006"},{"key":"ref_95","doi-asserted-by":"crossref","unstructured":"Melkamu, W.W., and Bitew, L.T. (2021). Green synthesis of silver nanoparticles using Hagenia abyssinica (Bruce) J.F. Gmel plant leaf extract and their antibacterial and anti-oxidant activities. Heliyon, 7.","DOI":"10.2139\/ssrn.3895676"},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"965","DOI":"10.1016\/j.talanta.2008.05.019","article-title":"Response surface methodology (RSM) as a tool for optimization in analytical chemistry","volume":"76","author":"Bezerra","year":"2008","journal-title":"Talanta"},{"key":"ref_97","doi-asserted-by":"crossref","unstructured":"Kesi\u0107, A., Markovi\u0107, K., Grujovi\u0107, M., and Markovi\u0107, Z. (2023). An investigation of the optimal conditions for the green synthesis of silver nanoparticles using an aqueous extract from the Agrimonia eupatoria L. plant. Mater. Proc., 14.","DOI":"10.3390\/IOCN2023-14477"},{"key":"ref_98","doi-asserted-by":"crossref","unstructured":"Manosalva, N., Tortella, G., Cristina Diez, M., Schalchli, H., Seabra, A.B., Dur\u00e1n, N., and Rubilar, O. (2019). Green synthesis of silver nanoparticles: Effect of synthesis reaction parameters on antimicrobial activity. World J. Microbiol. Biotechnol., 35.","DOI":"10.1007\/s11274-019-2664-3"},{"key":"ref_99","doi-asserted-by":"crossref","unstructured":"Ni, Q., Zhu, T., Wang, W., Guo, D., Li, Y., Chen, T., and Zhang, X. (2024). Green synthesis of narrow-size silver nanoparticles using Ginkgo biloba leaves: Condition optimization, characterization, and antibacterial and cytotoxic activities. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25031913"},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"279","DOI":"10.51470\/PLANTARCHIVES.2021.v21.no2.043","article-title":"Green synthesis of silver nanoparticles from fruit rind extract of Garcinia mangostana L. and evaluation of antibacterial properties","volume":"21","author":"Madhavan","year":"2021","journal-title":"Plant Arch."},{"key":"ref_101","doi-asserted-by":"crossref","unstructured":"Yegon, A.K., Oyetade, J.A., Mtavangu, S.G., Rwiza, M.J., and Machunda, R.L. (2025). Green synthesis of silver silver chloride (Ag\/AgCl) nanoparticles using macadamia nutshell xylan extract, characterization and evaluation of its antibacterial activity. Discov. Nano, 20.","DOI":"10.1186\/s11671-025-04192-z"},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"5281","DOI":"10.1007\/s12011-025-04566-5","article-title":"Therapeutic potential of green synthesized polyherbal formulated silver nanoparticles in alloxan-induced diabetes mellitus\u2014An in vivo strategy","volume":"203","author":"Afshin","year":"2025","journal-title":"Biol. Trace Elem. Res."},{"key":"ref_103","doi-asserted-by":"crossref","unstructured":"Zhang, C., Li, Y., Huo, Y., Wang, H., and Wang, D. (2025). Dianthus superbus L. (QM) Extract-Assisted Silver Nanoparticle Gelatin Films with Antioxidant and Antimicrobial Properties for Fresh Fruit Preservation. Foods, 14.","DOI":"10.3390\/foods14132327"},{"key":"ref_104","doi-asserted-by":"crossref","unstructured":"Wang, J., Guan, J., Ma, X., Zhao, D., Han, Y., Guo, D., Bai, J., Guo, Z., and Zhang, X. (2025). Artemisia argyi-Mediated Synthesis of Monodisperse Silver Nanoparticles as Components of Bioactive Nanofibrous Dressings with Dual Antibacterial and Regenerative Functions. J. Funct. Biomater., 16.","DOI":"10.3390\/jfb16070236"},{"key":"ref_105","doi-asserted-by":"crossref","unstructured":"Pote, S., Salve, P., and Kambi, M. (2025). One-pot green synthesis, optimization, and characterization of silver nanoparticles from Mitragyna parvifolia: A novel therapeutic strategy for rheumatoid arthritis targeting COX-2 and TNF-\u03b1. Bioorg. Chem., 163.","DOI":"10.1016\/j.bioorg.2025.108739"},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1016\/j.jfda.2017.07.005","article-title":"Silver nanoparticle biosynthesis by using phenolic acids in rice husk extract as reducing agents and dispersants","volume":"26","author":"Liu","year":"2018","journal-title":"J. Food Drug Anal."},{"key":"ref_107","doi-asserted-by":"crossref","unstructured":"Alwis, W.H.S., Murthy, V., Wang, H., Khandanlou, R., and Mandal, P.K. (2024). Green synthesis of Terminalia ferdinandiana Exell-mediated silver nanoparticles and evaluation of antibacterial performance. Biomolecules, 14.","DOI":"10.3390\/biom14121516"},{"key":"ref_108","doi-asserted-by":"crossref","unstructured":"Aldayel, M.F. (2024). Biofabrication of silver nanoparticles using Pergularia tomentosa extract and evaluation of their antibacterial, antioxidant, and cytotoxic properties. Life, 14.","DOI":"10.3390\/life14121639"},{"key":"ref_109","doi-asserted-by":"crossref","first-page":"34902","DOI":"10.1039\/D5RA05480J","article-title":"Synthesis of biogenic silver nanoparticles using Piper chaba leaf extract for dual-functional applications: Hg (ii) sensing and catalysis","volume":"15","author":"Akhe","year":"2025","journal-title":"RSC Adv."},{"key":"ref_110","doi-asserted-by":"crossref","unstructured":"Abada, E., Habib, F., Mashraqi, A., Modafer, Y., Alsolami, W., Ismail, K., Alamri, A.A., Mashlawi, A.M., and Shater, A.M. (2025). Eco-Friendly Synthesis and Characterization of Senna italica-Derived Silver Nanoparticles with Broad-Spectrum Antimicrobial Activity. Int. J. Microbiol., 2025.","DOI":"10.1155\/ijm\/2072594"},{"key":"ref_111","doi-asserted-by":"crossref","unstructured":"S\u00e1nchez-G\u00e1lvez, J., Mart\u00ednez-Isasi, S., G\u00f3mez-Salgado, J., Rumbo-Prieto, J.M., Sobrido-Prieto, M., S\u00e1nchez-Hern\u00e1ndez, M., Garc\u00eda-Mart\u00ednez, M., and Fern\u00e1ndez-Garc\u00eda, D. (2024). Cytotoxicity and concentration of silver ions released from dressings in the treatment of infected wounds: A systematic review. Front. Public Health, 12.","DOI":"10.3389\/fpubh.2024.1331753"},{"key":"ref_112","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1089\/wound.2020.1232","article-title":"Moist Wound Healing with Commonly Available Dressings","volume":"10","author":"Nuutila","year":"2021","journal-title":"Adv. Wound Care"},{"key":"ref_113","doi-asserted-by":"crossref","unstructured":"Gounden, V., and Singh, M. (2024). Hydrogels and Wound Healing: Current and Future Prospects. Gels, 10.","DOI":"10.3390\/gels10010043"},{"key":"ref_114","doi-asserted-by":"crossref","unstructured":"Alipour, S., Mirfardi, A., Seradj, S.H., Mohammadi, R., and Masjedi, M. (2025). Trigonella foenum-graecum L. (fenugreek) seed extract-loaded niosomal thin film as a wound dressing: Formulation, optimisation, in vitro and in vivo characterisation. Nat. Prod. Res., 1\u20139.","DOI":"10.1080\/14786419.2025.2511169"},{"key":"ref_115","doi-asserted-by":"crossref","unstructured":"Ho, T.C., Chang, C.C., Chan, H.P., Chung, T.W., Shu, C.W., Chuang, K.P., Duh, T.H., Yang, M.H., and Tyan, Y.C. (2022). Hydrogels: Properties and Applications in Biomedicine. Molecules, 27.","DOI":"10.3390\/molecules27092902"},{"key":"ref_116","doi-asserted-by":"crossref","first-page":"2472","DOI":"10.1002\/jbm.b.34048","article-title":"Sterilization of hydrogels for biomedical applications: A review","volume":"106","author":"Galante","year":"2018","journal-title":"J. Biomed. Mater. Res. B Appl. Biomater."},{"key":"ref_117","doi-asserted-by":"crossref","first-page":"218","DOI":"10.69613\/75xtdd94","article-title":"Review on Topical Gels as Drug Delivery System: Review Article","volume":"2","author":"Aishwarya","year":"2024","journal-title":"J. Pharma Insights Res."},{"key":"ref_118","doi-asserted-by":"crossref","unstructured":"Rajinikanth, B.S., Rajkumar, D.S.R., Keerthika, K., and Vijayaragavan, V. (2024). Chitosan-Based Biomaterial in Wound Healing: A Review. Cureus, 16.","DOI":"10.7759\/cureus.55193"},{"key":"ref_119","doi-asserted-by":"crossref","first-page":"5509","DOI":"10.1039\/D2RA07673J","article-title":"Biomedical materials for wound dressing: Recent advances and applications","volume":"13","author":"Nguyen","year":"2023","journal-title":"RSC Adv."},{"key":"ref_120","first-page":"1","article-title":"Silver Nanoparticle-Based Dressings for Various Wounds: Benefits and Adverse Effects","volume":"95","author":"Phatanodom","year":"2022","journal-title":"Pol. Przegl. Chir."},{"key":"ref_121","first-page":"14","article-title":"Dressing conformability and silver-containing wound dressings","volume":"6","author":"Bowler","year":"2010","journal-title":"Wounds"},{"key":"ref_122","doi-asserted-by":"crossref","unstructured":"R\u00fcther, L., and Voss, W. (2021). Hydrogel or ointment? Comparison of five different galenics regarding tissue breathability and transepidermal water loss. Heliyon, 7.","DOI":"10.1016\/j.heliyon.2021.e06071"},{"key":"ref_123","doi-asserted-by":"crossref","unstructured":"Sadeghi, M.M., Alipour, S., Morowvat, M.H., Salehi, M.S., Das, S.S., and Dehshahri, A. (2025). Green biosynthesized selenium nanoparticles using whole cell and cell lysate of Spirulina platensis: Characterizations and in vitro wound healing studies. BioNanoScience, 15.","DOI":"10.1007\/s12668-024-01753-x"},{"key":"ref_124","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1007\/5584_2024_817","article-title":"3D-Bioprinted Skin Tissues for Improving Wound Healing: Current Status and Perspective","volume":"1474","author":"Gopakumar","year":"2025","journal-title":"Adv. Exp. Med. Biol."},{"key":"ref_125","doi-asserted-by":"crossref","unstructured":"Truzzi, F., Tibaldi, C., Dilloo, S., Saltari, A., Levesque, M.P., Arcangeli, F., Garzi, A., Ruggiero, G., and Dinelli, G. (2025). Topical Administration of Vitamin D2 Combined with Colloidal Silver Nanoparticles Promotes Wound Repair and Protection Against Skin Irritation and UVB Irradiation in 3D Reconstructed Human Skin Models. Pharmaceutics, 17.","DOI":"10.3390\/pharmaceutics17040472"},{"key":"ref_126","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1111\/wrr.12513","article-title":"Non-animal models of wound healing in cutaneous repair: In silico, in vitro, ex vivo, and in vivo models of wounds and scars in human skin","volume":"25","author":"Bayat","year":"2017","journal-title":"Wound Repair Regen."},{"key":"ref_127","doi-asserted-by":"crossref","unstructured":"Ueck, C., Volksdorf, T., Houdek, P., Vidal, Y.S.S., Sehner, S., Ellinger, B., Lobmann, R., Larena-Avellaneda, A., Reinshagen, K., and Ridderbusch, I. (2017). Comparison of In-Vitro and Ex-Vivo Wound Healing Assays for the Investigation of Diabetic Wound Healing and Demonstration of a Beneficial Effect of a Triterpene Extract. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0169028"},{"key":"ref_128","doi-asserted-by":"crossref","unstructured":"Ahmad, N. (2022). In Vitro and In Vivo Characterization Methods for Evaluation of Modern Wound Dressings. Pharmaceutics, 15.","DOI":"10.3390\/pharmaceutics15010042"},{"key":"ref_129","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.wndm.2018.12.001","article-title":"Wound Healing Models; A Systematic Review of Animal and Non-Animal Models","volume":"24","author":"Sami","year":"2018","journal-title":"Wound Med."},{"key":"ref_130","doi-asserted-by":"crossref","unstructured":"Almuhanna, Y. (2025). Microbial biofilms as barriers to chronic wound healing: Diagnostic challenges and therapeutic advances. J. Clin. Med., 14.","DOI":"10.3390\/jcm14228121"},{"key":"ref_131","doi-asserted-by":"crossref","unstructured":"Ram\u00edrez-Rosas, S.L., Delgado-Alvarado, E., Sanchez-Vargas, L.O., Herrera-May, A.L., Pe\u00f1a-Juarez, M.G., and Gonzalez-Calderon, J.A. (2022). Green route to produce silver nanoparticles using the bioactive flavonoid quercetin as a reducing agent and food anti-caking agents as stabilizers. Nanomaterials, 12.","DOI":"10.3390\/nano12193545"},{"key":"ref_132","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1080\/24701556.2021.1891428","article-title":"Green synthesis of silver nanoparticles using gallic acid as reducing and capping agent: Effect of pH and gallic acid concentration on average particle size and stability","volume":"52","author":"Ahani","year":"2022","journal-title":"Inorg. Nano-Met. Chem."},{"key":"ref_133","doi-asserted-by":"crossref","unstructured":"Ali, A., Sattar, M., Hussain, F., Tareen, M.H.K., Militky, J., and Noman, M.T. (2021). Single-step green synthesis of highly concentrated and stable colloidal dispersion of core-shell silver nanoparticles and their antimicrobial and ultra-high catalytic properties. Nanomaterials, 11.","DOI":"10.20944\/preprints202104.0007.v1"},{"key":"ref_134","doi-asserted-by":"crossref","unstructured":"Jain, S., and Mehata, M.S. (2017). Medicinal plant leaf extract and pure flavonoid mediated green synthesis of silver nanoparticles and their enhanced antibacterial property. Sci. Rep., 7.","DOI":"10.1038\/s41598-017-15724-8"},{"key":"ref_135","first-page":"356","article-title":"Sustainable extraction and purification of phytochemicals: A review of green solvents and techniques","volume":"9","author":"Darwin","year":"2025","journal-title":"Chem. Methodol."},{"key":"ref_136","doi-asserted-by":"crossref","unstructured":"Xiong, Z., Liu, L., Zhang, Z., Cao, L., Cao, D., Du, Z., and Tang, Y. (2022). Unravelling the role of surface modification in the dermocompatibility of silver nanoparticles in vitro and in vivo. Chemosphere, 291.","DOI":"10.1016\/j.chemosphere.2021.133111"},{"key":"ref_137","doi-asserted-by":"crossref","first-page":"2295","DOI":"10.3390\/ma6062295","article-title":"Mechanisms of silver nanoparticle release, transformation and toxicity: A critical review of current knowledge and recommendations for future studies and applications","volume":"6","author":"Reidy","year":"2013","journal-title":"Materials"},{"key":"ref_138","doi-asserted-by":"crossref","unstructured":"Alaroud, A.A., Bani Melhim, S., Banat, F., Husaini, A., Abudoleh, S.M., Alkawareek, M.Y., and Alkilany, A.M. (2026). Matrix-Dependent Stability and Antibacterial Efficacy of Silver Nanoparticles: A Comparative Study of Anionic Carbopol vs. Non-Ionic Pluronic Hydrogels. Pharmaceutics, 18.","DOI":"10.3390\/pharmaceutics18030314"},{"key":"ref_139","doi-asserted-by":"crossref","unstructured":"Lee, C.K., Wang, F.T., Huang, C.H., and Chan, W.H. (2024). Dose-dependent effects of silver nanoparticles on cell death modes in mouse blastocysts induced via endoplasmic reticulum stress and mitochondrial apoptosis. Toxicol. Res., 13.","DOI":"10.1093\/toxres\/tfae158"},{"key":"ref_140","doi-asserted-by":"crossref","unstructured":"Percie du Sert, N., Ahluwalia, A., Alam, S., Avey, M.T., Baker, M., Browne, W.J., Clark, A., Cuthill, I.C., Dirnagl, U., and Emerson, M. (2020). Reporting animal research: Explanation and elaboration for the ARRIVE guidelines 2.0. PLoS Biol., 18.","DOI":"10.1371\/journal.pbio.3000411"},{"key":"ref_141","doi-asserted-by":"crossref","first-page":"1007","DOI":"10.1177\/0300985813485099","article-title":"Principles for valid histopathologic scoring in research","volume":"50","author":"Olivier","year":"2013","journal-title":"Vet. Pathol."},{"key":"ref_142","doi-asserted-by":"crossref","unstructured":"Hooijmans, C.R., Rovers, M.M., De Vries, R.B., Leenaars, M., Ritskes-Hoitinga, M., and Langendam, M.W. (2014). SYRCLE\u2019s risk of bias tool for animal studies. BMC Med. Res. Methodol., 14.","DOI":"10.1186\/1471-2288-14-43"},{"key":"ref_143","unstructured":"U.S. Food and Drug Administration (2011). How to Write a Request for Designation (RFD): Guidance for Industry."},{"key":"ref_144","unstructured":"U.S. Food and Drug Administration (2022). Drug Products, Including Biological Products, That Contain Nanomaterials: Guidance for Industry."},{"key":"ref_145","unstructured":"(2025). Biological Evaluation of Medical Devices\u2014Part 1: Requirements and General Principles for the Evaluation of Biological Safety within a Risk Management Process (Standard No. ISO 10993-1:2025)."},{"key":"#cr-split#-ref_146.1","unstructured":"European Parliament, and Council of the European Union (2017). Regulation"},{"key":"#cr-split#-ref_146.2","unstructured":"(EU) 2017\/745 of the European Parliament and of the Council on Medical Devices. Off. J. Eur. Union, 60, 1-175."}],"container-title":["Pharmaceutics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4923\/18\/9\/1161\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T18:28:33Z","timestamp":1789496913000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4923\/18\/9\/1161"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,15]]},"references-count":147,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["pharmaceutics18091161"],"URL":"https:\/\/doi.org\/10.3390\/pharmaceutics18091161","relation":{},"ISSN":["1999-4923"],"issn-type":[{"value":"1999-4923","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,15]]}}}