{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T20:17:01Z","timestamp":1789676221635,"version":"4.0.1"},"reference-count":33,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2021,2,9]],"date-time":"2021-02-09T00:00:00Z","timestamp":1612828800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Artemisia argyi is widely used as traditional medicine in East Asia. However, its effects against inflammation and gastric ulcers have not been reported yet. We analyzed anti-inflammatory activity and its molecular mechanisms of A. argyi using RAW264.7 cells line, then evaluated the curative efficacy in rats with acute gastric ulcers. Nitric oxide and IL-6 production was measured using Griess reagent and an ELISA kit. Inducible nitric oxide synthase (iNOS), interleukin (IL)-6, and mucin (MUC)1, MUC5AC, and MUC6 mRNA were determined by SYBR Green or Taqman qRT-PCR methods. The phosphorylation of ERK, JNK, p38, and c-Jun protein were detected by western blotting. RW0117 inhibited LPS-induced NO and IL-6 production. The mRNA levels of iNOS and IL-6 were strongly suppressed. The phosphorylation of ERK, JNK, and c-Jun decreased by treatment with RW0117. Oral administration of RW0117 recovered the amount of mucin mRNA and protein level that was decreased due to gastric ulcers by HCl-EtOH. A. argyi exhibited strong anti-inflammatory effects and contributed to the modulation of HCl-EtOH-induced gastric ulcer in rats.<\/jats:p>","DOI":"10.3390\/plants10020332","type":"journal-article","created":{"date-parts":[[2021,2,10]],"date-time":"2021-02-10T04:33:46Z","timestamp":1612931626000},"page":"332","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Anti-Inflammatory Effect of Artemisia argyi on Ethanol-Induced Gastric Ulcer: Analytical, In Vitro and In Vivo Studies for the Identification of Action Mechanism and Active Compounds"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5103-437X","authenticated-orcid":false,"given":"Myoung-Sook","family":"Shin","sequence":"first","affiliation":[{"name":"Korean Medicine, Gachon University, Seongnam-si, Gyeonggi-do 13120, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaemin","family":"Lee","sequence":"additional","affiliation":[{"name":"Korean Medicine, Gachon University, Seongnam-si, Gyeonggi-do 13120, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin Woo","family":"Lee","sequence":"additional","affiliation":[{"name":"Research &amp; Development Center, Richwood Pharmaceuticals, 1, Gwanak-gu, Seoul 08826, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Se Hoon","family":"Park","sequence":"additional","affiliation":[{"name":"Research &amp; Development Center, Richwood Pharmaceuticals, 1, Gwanak-gu, Seoul 08826, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Il Kyun","family":"Lee","sequence":"additional","affiliation":[{"name":"Research &amp; Development Center, Richwood Pharmaceuticals, 1, Gwanak-gu, Seoul 08826, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jung A.","family":"Choi","sequence":"additional","affiliation":[{"name":"Research &amp; Development Center, Richwood Pharmaceuticals, 1, Gwanak-gu, Seoul 08826, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jung Suk","family":"Lee","sequence":"additional","affiliation":[{"name":"Research &amp; Development Center, Richwood Pharmaceuticals, 1, Gwanak-gu, Seoul 08826, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ki Sung","family":"Kang","sequence":"additional","affiliation":[{"name":"Korean Medicine, Gachon University, Seongnam-si, Gyeonggi-do 13120, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,2,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1097\/MCC.0b013e3280106806","article-title":"The impact of endogenous triggers on trauma-associated inflammation","volume":"12","author":"Zedler","year":"2006","journal-title":"Curr. Opin. Crit. Care"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"499","DOI":"10.3109\/07853899709007474","article-title":"Pro- and anti-inflammatory cytokines in rheumatoid arthritis","volume":"29","author":"Punnonen","year":"1997","journal-title":"Ann. Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2046","DOI":"10.1073\/pnas.91.6.2046","article-title":"Inducible isoforms of cyclooxygenase and nitric-oxide synthase in inflammation","volume":"91","author":"Vane","year":"1994","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1425","DOI":"10.4049\/jimmunol.144.4.1425","article-title":"Cytolytic mechanisms of activated macrophages. Tumor necrosis factor and Larginine-dependent mechanisms act synergistically as the major cytolytic mechanisms of activated macrophages","volume":"144","author":"Higuchi","year":"1990","journal-title":"J. Immunol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"3051","DOI":"10.1096\/fasebj.6.12.1381691","article-title":"Nitric oxide as a secretory product of mammalian cells","volume":"6","author":"Nathan","year":"1992","journal-title":"FASEB J."},{"key":"ref_6","first-page":"469","article-title":"Nitric oxide and superoxide in inflammation and immune regulation","volume":"54","author":"Guzik","year":"2003","journal-title":"J. Physiol. Pharmacol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1159\/000090167","article-title":"Alcohol-related diseases of the esophagus and stomach","volume":"23","author":"Franke","year":"2005","journal-title":"Dig. Dis."},{"key":"ref_8","first-page":"131","article-title":"Unidentified curved baciliin the stomach of patients with gastritis and peptic ulceration","volume":"1","author":"Marshal","year":"1984","journal-title":"Lancet"},{"key":"ref_9","first-page":"425","article-title":"Development of Helicobacter pylori-induced gastric carcinoma in Mongolian gerbils","volume":"58","author":"Honda","year":"1998","journal-title":"Cancer Res."},{"key":"ref_10","first-page":"701","article-title":"Gastroduodenal mucosal defence and mucosal protective agents","volume":"29","author":"Goel","year":"1991","journal-title":"Indian J. Exp. Biol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1038\/nrgastro.2009.125","article-title":"Insights into the future of gastric acid suppression","volume":"6","author":"DeVault","year":"2009","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1016\/j.alcohol.2008.08.009","article-title":"Gastroprotective effect of cyanidin 3-glucoside on ethanol-induced gastric lesions in rats","volume":"42","author":"Li","year":"2008","journal-title":"Alcohol"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"24166","DOI":"10.1038\/srep24166","article-title":"The caspase-1 inhibitor AC-YVAD-CMK attenuates acute gastric injury in mice: Involvement of silencing NLRP3 inflammasome activities","volume":"6","author":"Zhang","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.taap.2013.05.035","article-title":"Protective effect of tetrahydrocoptisine against ethanol-induced gastric ulcer in mice","volume":"272","author":"Li","year":"2013","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1016\/j.jep.2005.01.011","article-title":"Allophylus serratus: A plant with potential anti-ulcerogenic activity","volume":"99","author":"Vinay","year":"2005","journal-title":"J. Ethnopharmacol."},{"key":"ref_16","first-page":"1867","article-title":"Anti-ulcer effect of Aloe vera in non-steroidal anti-inflammatory drug induced peptic ulcers in rats","volume":"5","author":"Sai","year":"2011","journal-title":"Afr. J. Pharm. Pharmacol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1093\/abbs\/gmy002","article-title":"Kaempferol protects ethanol-induced gastric ulcers in mice via pro-inflammatory cytokines and NO","volume":"50","author":"Li","year":"2018","journal-title":"Acta. Biochim. Biophys. Sin."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Yun, C., Jung, Y., Chun, W., Yang, B., Ryu, J., Lim, C., and Cho, S.I. (2016). Anti-inflammatory effects of Artemisia leaf extract in mice with contact dermatitis in vitro and in vivo. Mediat. Inflamm.","DOI":"10.1155\/2016\/8027537"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.jep.2017.07.033","article-title":"Artemisia argyi attenuates airway inflammation in ovalbumin-induced asthmatic animals","volume":"209","author":"Shin","year":"2017","journal-title":"J. Ethnopharmacol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"402","DOI":"10.3389\/fphar.2020.00402","article-title":"Immunosuppressive Activity of Artemisia argyi Extract and Isolated Compounds","volume":"11","author":"Reinhardt","year":"2020","journal-title":"Front. Pharmacol."},{"key":"ref_21","first-page":"1741","article-title":"Polyphenol mixture of a native Korean variety of Artemisia argyi H. (Seomae mugwort) and its anti-inflammatory effects","volume":"44","author":"Kim","year":"2019","journal-title":"Int. J. Mol. Med."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1121","DOI":"10.1016\/j.lfs.2011.04.011","article-title":"Eupatilin inhibits lipopolysaccharide-induced expression of inflammatory mediators in macrophages","volume":"88","author":"Choi","year":"2011","journal-title":"Life Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1016\/j.jep.2009.06.001","article-title":"Inhibitory effect of eupatilin and jaceosidin isolated from Artemisia princeps on carrageenan-induced inflammation in mice","volume":"125","author":"Min","year":"2009","journal-title":"J. Ethnopharmacol."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Kim, D.E., Min, K.J., Kim, M.J., Kim, S.H., and Kwon, T.K. (2019). Hispidulin inhibits mast cell-mediated allergic inflammation through down-regulation of histamine release and inflammatory cytokines. Molecules, 24.","DOI":"10.3390\/molecules24112131"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"4652","DOI":"10.1021\/acs.jafc.8b00152","article-title":"Stimulation of innate immune function by panax ginseng after heat processing","volume":"66","author":"Shin","year":"2018","journal-title":"J. Agric. Food Chem."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Shin, M.S., Kim, S.B., Lee, J., Choi, H.S., Park, J., Park, J.Y., and Kang, K.S. (2018). Beneficial effect of herbal formulation KM1608 on inflammatory bowel diseases: A preliminary experimental study. Molecules, 23.","DOI":"10.3390\/molecules23082068"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"67","DOI":"10.3904\/kjim.2018.290","article-title":"Gastroprotective effects of irsogladine maleate on ethanol\/hydrochloric acid induced gastric ulcers in mice","volume":"36","author":"Kwon","year":"2021","journal-title":"Korean J. Intern. Med."},{"key":"ref_28","first-page":"147","article-title":"Effects of Abexol (beeswax alcohols) on gastrointestinal symptoms in middle-aged and older subjects","volume":"40","author":"Illnait","year":"2009","journal-title":"Rev. CENIC Cienc. Biol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"648","DOI":"10.1016\/j.jff.2018.11.029","article-title":"Phytochemical components and biological activities of Artemisia Argyi","volume":"52","author":"Song","year":"2019","journal-title":"J. Funct. Foods"},{"key":"ref_30","first-page":"1","article-title":"Characterization of nutritional composition, antioxidative capacity, and sensory attributes of Seomae mugwort, a native Korean variety of Artemisia argyi H. Lev. & Vaniot","volume":"2015","author":"Kim","year":"2015","journal-title":"J. Anal. Methods Chem."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1055\/s-2003-38486","article-title":"Antitumor activity of flavones isolated from Artemisia argyi","volume":"69","author":"Seo","year":"2003","journal-title":"Planta Med."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"632","DOI":"10.1080\/14786419.2017.1332603","article-title":"Two new flavonoids from Artemisia argyi with their anticoagulation activities","volume":"32","author":"Lv","year":"2018","journal-title":"Nat. Prod. Res."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"939","DOI":"10.1111\/jpn.12895","article-title":"Immunomodulatory effect of Artemisia argyi polysaccharide on peripheral blood leucocyte of broiler chickens","volume":"102","author":"Zhang","year":"2018","journal-title":"J. Anim. Physiol. Anim. Nutr."}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/10\/2\/332\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T05:21:50Z","timestamp":1760160110000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/10\/2\/332"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,2,9]]},"references-count":33,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2021,2]]}},"alternative-id":["plants10020332"],"URL":"https:\/\/doi.org\/10.3390\/plants10020332","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,2,9]]}}}