{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T06:41:40Z","timestamp":1787985700424,"version":"build-2784847793"},"reference-count":65,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2021,2,23]],"date-time":"2021-02-23T00:00:00Z","timestamp":1614038400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Eysenhardtia platycarpa (Fabaceae) is a medicinal plant used in Mexico. Biotechnological studies of its use are lacking. The objective of this work was to establish a cell suspension culture (CSC) of E. platycarpa, determine the phytochemical constituents by spectrophotometric and gas chromatography\u2012mass spectrometry (GC\u2012MS) methods, evaluate its antifungal activity, and compare them with the intact plant. Friable callus and CSC were established with 2 mg\/L 1-naphthaleneacetic acid plus 0.1 mg\/L kinetin. The highest total phenolics of CSC was 15.6 mg gallic acid equivalents (GAE)\/g dry weight and the total flavonoids content ranged from 56.2 to 104.1 \u00b5g quercetin equivalents (QE)\/g dry weight. The GC\u2012MS analysis showed that the dichloromethane extracts of CSC, sapwood, and heartwood have a high amount of hexadecanoic acid (22.3\u201335.3%) and steroids (13.5\u201314.7%). Heartwood and sapwood defatted hexane extracts have the highest amount of stigmasterol (~23.4%) and \u03b2-sitosterol (~43%), and leaf extracts presented \u03b2-amyrin (16.3%). Methanolic leaf extracts showed mostly sugars and some polyols, mainly D-pinitol (74.3%). Compared with the intact plant, dichloromethane and fatty hexane extracts of CSC exhibited percentages of inhibition higher for Sclerotium cepivorum: 71.5% and 62.0%, respectively. The maximum inhibition for Rhizoctonia solani was with fatty hexane extracts of the sapwood (51.4%). Our study suggests that CSC extracts could be used as a possible complementary alternative to synthetic fungicides.<\/jats:p>","DOI":"10.3390\/plants10020414","type":"journal-article","created":{"date-parts":[[2021,2,23]],"date-time":"2021-02-23T12:40:16Z","timestamp":1614084016000},"page":"414","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Establishment of a Cell Suspension Culture of Eysenhardtia platycarpa: Phytochemical Screening of Extracts and Evaluation of Antifungal Activity"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2811-1143","authenticated-orcid":false,"given":"Antonio","family":"Bernab\u00e9-Antonio","sequence":"first","affiliation":[{"name":"Departamento de Madera, Celulosa y Papel, Centro Universitario de Ciencias Exactas e Ingenier\u00edas, Universidad de Guadalajara, Km 15.5 Carretera Guadalajara-Nogales, Col. Las Agujas, Zapopan 45100, Jalisco, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandro","family":"S\u00e1nchez-S\u00e1nchez","sequence":"additional","affiliation":[{"name":"Departamento de Madera, Celulosa y Papel, Centro Universitario de Ciencias Exactas e Ingenier\u00edas, Universidad de Guadalajara, Km 15.5 Carretera Guadalajara-Nogales, Col. Las Agujas, Zapopan 45100, Jalisco, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Romero-Estrada","sequence":"additional","affiliation":[{"name":"Departamento de Madera, Celulosa y Papel, Centro Universitario de Ciencias Exactas e Ingenier\u00edas, Universidad de Guadalajara, Km 15.5 Carretera Guadalajara-Nogales, Col. Las Agujas, Zapopan 45100, Jalisco, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan Carlos","family":"Meza-Contreras","sequence":"additional","affiliation":[{"name":"Departamento de Madera, Celulosa y Papel, Centro Universitario de Ciencias Exactas e Ingenier\u00edas, Universidad de Guadalajara, Km 15.5 Carretera Guadalajara-Nogales, Col. Las Agujas, Zapopan 45100, Jalisco, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Antonio","family":"Silva-Guzm\u00e1n","sequence":"additional","affiliation":[{"name":"Departamento de Madera, Celulosa y Papel, Centro Universitario de Ciencias Exactas e Ingenier\u00edas, Universidad de Guadalajara, Km 15.5 Carretera Guadalajara-Nogales, Col. Las Agujas, Zapopan 45100, Jalisco, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco Javier","family":"Fuentes-Talavera","sequence":"additional","affiliation":[{"name":"Departamento de Madera, Celulosa y Papel, Centro Universitario de Ciencias Exactas e Ingenier\u00edas, Universidad de Guadalajara, Km 15.5 Carretera Guadalajara-Nogales, Col. Las Agujas, Zapopan 45100, Jalisco, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2195-2169","authenticated-orcid":false,"given":"Israel","family":"Hurtado-D\u00edaz","sequence":"additional","affiliation":[{"name":"Departamento de Madera, Celulosa y Papel, Centro Universitario de Ciencias Exactas e Ingenier\u00edas, Universidad de Guadalajara, Km 15.5 Carretera Guadalajara-Nogales, Col. Las Agujas, Zapopan 45100, Jalisco, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7464-9898","authenticated-orcid":false,"given":"Laura","family":"Alvarez","sequence":"additional","affiliation":[{"name":"Centro de Investigaciones Qu\u00edmicas-IICBA, Universidad Aut\u00f3noma del Estado de Morelos, Av. Universidad No. 1001, Col. Chamilpa, Cuernavaca 62209, Morelos, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7127-7264","authenticated-orcid":false,"given":"Francisco","family":"Cruz-Sosa","sequence":"additional","affiliation":[{"name":"Departamento de Biotecnolog\u00eda, Divisi\u00f3n de Ciencias Biol\u00f3gicas y de la Salud, Universidad Aut\u00f3noma Metropolitana-Unidad Iztapalapa, San Rafael Atlixco 186, Col. Vicentina, Ciudad de M\u00e9xico 09340, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,2,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1590\/S1678-91992010000300006","article-title":"Biological properties of medicinal plants: A review of their antimicrobial activity","volume":"16","author":"Silva","year":"2010","journal-title":"J. Venom. Anim. Toxins"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.cropro.2018.04.003","article-title":"Formulation of botanicals for the control of plant-pathogens: A review","volume":"110","author":"Borges","year":"2018","journal-title":"Crop Prot."},{"key":"ref_3","first-page":"29","article-title":"Plant cell cultures\u2014An alternative and efficient source for the production of biologically important secondary metabolites","volume":"2","author":"Mulabagal","year":"2004","journal-title":"Int. J. Appl. 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