{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T16:44:23Z","timestamp":1791477863750,"version":"4.3.4"},"reference-count":45,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2021,3,23]],"date-time":"2021-03-23T00:00:00Z","timestamp":1616457600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["PTDC\/ASP-PLA\/31946\/2017, POCI-01-0145-FEDER-031946"],"award-info":[{"award-number":["PTDC\/ASP-PLA\/31946\/2017, POCI-01-0145-FEDER-031946"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["CEECIND\/02082\/2017"],"award-info":[{"award-number":["CEECIND\/02082\/2017"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>The root lesion Pratylenchus penetrans is an economically important pest affecting a wide range of plants. The morphometry of five P. penetrans isolates, parasitizing potato roots in Portugal, was compared and variability within and between isolates was observed. Of the 15 characters assessed, vulva position (V%) in females and the stylet length in both females\/males showed the lowest coefficient of intra and inter-isolate variability. Moreover, DNA sequencing of the internal transcribed spacers (ITS) genomic region and cytochrome c oxidase subunit 1 (COI) gene was performed, in order to evaluate the intraspecific genetic variability of this species. ITS revealed higher isolate genetic diversity than the COI gene, with 15 and 7 different haplotypes from the 15 ITS and 14 COI sequences, respectively. Intra- and inter-isolate genetic diversity was found considering both genomic regions. The differentiation of these isolates was not related with their geographical origin. In spite of the high intraspecific variability, phylogenetic analyses revealed that both ITS region and COI gene separate P. penetrans from other related species. Our findings contribute to increasing the understanding of P. penetrans variability.<\/jats:p>","DOI":"10.3390\/plants10030603","type":"journal-article","created":{"date-parts":[[2021,3,23]],"date-time":"2021-03-23T23:59:41Z","timestamp":1616543981000},"page":"603","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Pratylenchus penetrans Parasitizing Potato Crops: Morphometric and Genetic Variability of Portuguese Isolates"],"prefix":"10.3390","volume":"10","author":[{"given":"Diogo","family":"Gil","sequence":"first","affiliation":[{"name":"Centre of Functional Ecology, Department of Life Sciences, University of Coimbra, Cal\u00e7ada Martim de Freitas, 3000-456 Coimbra, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6594-4892","authenticated-orcid":false,"given":"Joana M.S.","family":"Cardoso","sequence":"additional","affiliation":[{"name":"Centre of Functional Ecology, Department of Life Sciences, University of Coimbra, Cal\u00e7ada Martim de Freitas, 3000-456 Coimbra, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabel","family":"Abrantes","sequence":"additional","affiliation":[{"name":"Centre of Functional Ecology, Department of Life Sciences, University of Coimbra, Cal\u00e7ada Martim de Freitas, 3000-456 Coimbra, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4944-6789","authenticated-orcid":false,"given":"Iv\u00e2nia","family":"Esteves","sequence":"additional","affiliation":[{"name":"Centre of Functional Ecology, Department of Life Sciences, University of Coimbra, Cal\u00e7ada Martim de Freitas, 3000-456 Coimbra, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,3,23]]},"reference":[{"key":"ref_1","first-page":"27","article-title":"A new parasitic nema found infesting cotton and potatoes","volume":"11","author":"Cobb","year":"1917","journal-title":"J. Agric. Res."},{"key":"ref_2","unstructured":"Filipjev, I.N., and Stekhoven, J.H.S. (1941). A Manual of Agricultural Helminthology, Brill."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Castillo, P., and Vovlas, N. (2007). Pratylenchus (Nematoda: Pratylenchidae): Diagnosis, Biology, Pathogenicity and Management, Brill.","DOI":"10.1163\/ej.9789004155640.i-523"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1094\/Phyto-75-412","article-title":"Synergistic interactions between Verticillium dahliae and Pratylenchus penetrans in potato early dying disease","volume":"75","author":"Rowe","year":"1985","journal-title":"Phytopathology"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1007\/BF02852173","article-title":"Root lesion nematodes in potatoes","volume":"56","author":"Kimpinski","year":"1979","journal-title":"Am. Potato J."},{"key":"ref_6","first-page":"189","article-title":"Species of Pratylenchus associated with Solanum tuberosum cv. Superior in Ohio","volume":"12","author":"Brown","year":"1980","journal-title":"J. Nematol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1080\/07060668209501283","article-title":"Plant-parasitic nematodes associated with potatoes (Solanum tuberosum L.) in Simcoe County, Ontario","volume":"4","author":"Olthof","year":"1982","journal-title":"Can. J. Plant Pathol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1077","DOI":"10.1094\/Phyto-80-1077","article-title":"Role of Pratylenchus penetrans in potato early dying disease of Russet Burbank potato","volume":"80","author":"MacGuidwin","year":"1990","journal-title":"Phytopathology"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1094\/PD-89-0207B","article-title":"First report of stunting and root rot of potato associated with Pratylenchus penetrans in the Columbia Basin of Washington","volume":"89","author":"Ingham","year":"2005","journal-title":"Plant Dis."},{"key":"ref_10","first-page":"25","article-title":"Field damage in potato by lesion nematode Pratylenchus penetrans, its association with tuber symptoms and its survival in storage","volume":"37","author":"Holgado","year":"2009","journal-title":"Nematol. Mediterr."},{"key":"ref_11","first-page":"91","article-title":"Occurrence and distribution of nematodes in Idaho crops","volume":"20","author":"Hafez","year":"2010","journal-title":"Int. J. Nematol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1007\/s10658-014-0551-1","article-title":"Root-lesion and root-knot nematodes parasitizing potato","volume":"141","author":"Esteves","year":"2015","journal-title":"Eur. J. Plant Pathol."},{"key":"ref_13","first-page":"363","article-title":"Morphology and morphometrics of six species of Pratylenchus","volume":"1","author":"Roman","year":"1969","journal-title":"J. Nematol."},{"key":"ref_14","first-page":"185","article-title":"Morphological variation in Pratylenchus penetrans","volume":"8","author":"Tarte","year":"1976","journal-title":"J. Nematol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.ympev.2017.07.027","article-title":"Molecular characterization and species delimiting of plant-parasitic nematodes of the genus Pratylenchus from the Penetrans group (Nematoda: Pratylenchidae)","volume":"117","author":"Janssen","year":"2017","journal-title":"Mol. Phylogenet. Evol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1163\/156854100509024","article-title":"Molecular characterisation of 18 Pratylenchus species using rDNA restriction fragment length polymorphism","volume":"2","author":"Waeyenberge","year":"2000","journal-title":"Nematology"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1007\/s10658-011-9763-9","article-title":"Molecular variability and phylogenetic relationships among different species and populations of Pratylenchus (Nematoda: Pratylenchidae) as inferred from the analysis of the ITS rDNA","volume":"130","author":"Reyes","year":"2011","journal-title":"Eur. J. Plant Pathol."},{"key":"ref_18","first-page":"687","article-title":"Phylogenetic analysis of Pratylenchus (Nematoda, Pratylenchidae) based on ribosomal internal transcribed spacers (ITS) and D2-D3 expansion segments of 28S rRNA gene","volume":"37","author":"Wang","year":"2012","journal-title":"Acta Zootaxonomica Sin."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1016\/j.ympev.2008.04.028","article-title":"A phylogenetic framework for root lesion nematodes of the genus Pratylenchus (Nematoda): Evidence from 18S and D2\u2013D3 expansion segments of 28S ribosomal RNA genes and morphological characters","volume":"48","author":"Subbotin","year":"2008","journal-title":"Mol. Phylogenet. Evol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"927","DOI":"10.1163\/156854109X456862","article-title":"A phylogenetic tree of nematodes based on about 1200 full-length small subunit ribosomal DNA sequences","volume":"11","author":"Holterman","year":"2009","journal-title":"Nematology"},{"key":"ref_21","first-page":"142","article-title":"Discrimination of six Pratylenchus species using PCR and species-specific primers","volume":"36","author":"Ploeg","year":"2004","journal-title":"J. Nematol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1163\/156854101753389220","article-title":"Morphological and molecular characterisation of Pratylenchus arlingtoni n. sp., P. convallariae and P. fallax (Nematoda: Pratylenchidae)","volume":"3","author":"Handoo","year":"2001","journal-title":"Nematol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1007\/s10658-014-0443-4","article-title":"Morphological and molecular characterisation of Pratylenchus oleae n. sp. (Nematoda: Pratylenchidae) parasitizing wild and cultivated olives in Spain and Tunisia","volume":"140","author":"Guesmi","year":"2014","journal-title":"Eur. J. Plant Pathol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1163\/15685411-00002981","article-title":"Characterisation of amphimictic and parthenogenetic populations of Pratylenchus bolivianus Corbett, 1983 (Nematoda: Pratylenchidae) and their phylogenetic relationships with closely related species","volume":"18","author":"Troccoli","year":"2016","journal-title":"Nematology"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1163\/15685411-00003175","article-title":"Morphological and molecular characterisation of Pratylenchus rwandae n. sp. (Tylenchida: Pratylenchidae) associated with maize in Rwanda","volume":"20","author":"Singh","year":"2018","journal-title":"Nematology"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"257","DOI":"10.2478\/s11756-018-00165-z","article-title":"Molecular and morphological variation of the root-lesion nematode Pratylenchus neglectus","volume":"74","author":"Divsalar","year":"2019","journal-title":"Biologia"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1007\/s10658-019-01792-1","article-title":"Molecular variability of the root-lesion nematode, Pratylenchus loosi (Nematoda: Pratylenchidae), from tea in Iran","volume":"155","author":"Mirghasemi","year":"2019","journal-title":"Eur. J. Plant Pathol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"987","DOI":"10.1163\/156854112X638424","article-title":"Pratylenchus speijeri n. sp. (Nematoda: Pratylenchidae), a new root-lesion nematode pest of plantain in West Africa","volume":"14","author":"Troccoli","year":"2012","journal-title":"Nematology"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1007\/s10658-018-1480-1","article-title":"Sequence variation in ribosomal DNA and in the nuclear hsp90 gene of Pratylenchus penetrans (Nematoda: Pratylenchidae) populations and phylogenetic analysis","volume":"152","author":"Fanelli","year":"2018","journal-title":"Eur. J. Plant Pathol."},{"key":"ref_30","first-page":"193","article-title":"Molecular, morphological and thermal characters of 19 Pratylenchus spp. and relatives using the D3 segment of the nuclear LSU rRNA gene","volume":"31","author":"Carta","year":"2001","journal-title":"Nematropica"},{"key":"ref_31","first-page":"29","article-title":"Taxonomic studies on the genus Pratylenchus (Nematoda)","volume":"66","author":"Loof","year":"1960","journal-title":"Tijdschrift Plantenziekten"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Rusinque, L.C.M., Vicente, C., In\u00e1cio, M.L.S., N\u00f3brega, F., Camacho, M.J., Lima, A., and Ramos, A.P. (2020). First report of Pratylenchus penetrans (Nematoda: Pratylenchida) associated with Amaryllis (Hippeastrum \u00d7 hybridum), in Portugal. Plant Dis., 1\u20135.","DOI":"10.1094\/PDIS-03-20-0524-PDN"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1163\/15685411-00002993","article-title":"Diversity of root-lesion nematodes (Pratylenchus spp.) associated with wheat (Triticum aestivum and T. durum) in Morocco","volume":"18","author":"Mokrini","year":"2016","journal-title":"Nematology"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1007\/s40858-015-0013-0","article-title":"Characterization of Brazilian populations of Pratylenchus brachyurus using morphological and molecular analyses","volume":"40","author":"Machado","year":"2015","journal-title":"Trop. Plant Pathol."},{"key":"ref_35","first-page":"205","article-title":"Morphological observations of Pratylenchus penetrans from celery and strawberry in southern Ontario","volume":"23","author":"Townshend","year":"1991","journal-title":"J. Nematol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1051\/fruits:2008028","article-title":"In vitro rearing of Pratylenchidae nematodes on carrot discs","volume":"63","author":"Boisseau","year":"2008","journal-title":"Fruits"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"91","DOI":"10.3354\/meps300091","article-title":"Mitochondrial DNA variation and cryptic speciation within the free-living marine nematode Pellioditis marina","volume":"300","author":"Derycke","year":"2005","journal-title":"Mar. Ecol. Prog. Ser."},{"key":"ref_38","first-page":"95","article-title":"BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95\/98\/NT","volume":"41","author":"Hall","year":"1999","journal-title":"Nucleic Acids Symp. Ser."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"3389","DOI":"10.1093\/nar\/25.17.3389","article-title":"Gapped BLAST and PSI-BLAST: A new generation of protein database search programs","volume":"25","author":"Altschul","year":"1997","journal-title":"Nucleic Acids Res."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"3299","DOI":"10.1093\/molbev\/msx248","article-title":"DnaSP 6: DNA sequence polymorphism analysis of large data sets","volume":"34","author":"Rozas","year":"2017","journal-title":"Mol. Biol. Evol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1093\/molbev\/msy096","article-title":"MEGA X: Molecular evolutionary genetics analysis across computing platforms","volume":"35","author":"Kumar","year":"2018","journal-title":"Mol. Biol. Evol."},{"key":"ref_42","first-page":"406","article-title":"The neighbor-joining method: A new method for reconstructing phylogenetic trees","volume":"4","author":"Saitou","year":"1987","journal-title":"Mol. Biol. Evol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"11030","DOI":"10.1073\/pnas.0404206101","article-title":"Prospects for inferring very large phylogenies by using the neighbor-joining method","volume":"101","author":"Tamura","year":"2004","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1093\/genetics\/123.3.585","article-title":"Statistical method for testing the neutral mutation hypothesis by DNA polymorphism","volume":"123","author":"Tajima","year":"1989","journal-title":"Genetics"},{"key":"ref_45","unstructured":"Wessa, P. (2021, January 10). Kendall tau Rank Correlation (v1.0.13) in Free Statistics Software (v1.2.1). Available online: https:\/\/www.wessa.net\/rwasp_kendall.wasp\/."}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/10\/3\/603\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T05:39:42Z","timestamp":1760161182000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/10\/3\/603"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,3,23]]},"references-count":45,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2021,3]]}},"alternative-id":["plants10030603"],"URL":"https:\/\/doi.org\/10.3390\/plants10030603","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,3,23]]}}}