{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T10:15:39Z","timestamp":1788862539010,"version":"build-2803163510"},"reference-count":48,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2021,3,30]],"date-time":"2021-03-30T00:00:00Z","timestamp":1617062400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>One of the most important compounds that exhibit a wide range of biological activities with especially strong antioxidant action are plant polyphenols. In the course of the experiment, the dose-dependent effects of polyphenols-rich extracts isolated from the Lamiaceae family Kazakhstani plants were studied on the processes of lipid peroxidation and on the degree of erythrocytes hemolysis. The activity of aqueous-ethanolic extracts from dried parts of plants, such as Origanum vulgare, Ziziphora bungeana, Dracocephalum integrifolium, Mentha piperita, Leonurus turkestanicus, Thymus serpyllum, and Salvia officinalis, was studied in a Wistar rat model. Lipid peroxidation (LPO) in liver microsomes was assessed by measuring malondialdehyde content in the form of thiobarbituric acid-reacting substances (TBARS). Estimation of osmotic resistance of isolated erythrocytes was evaluated based on hemoglobin absorbance. The amount of total phenolics in the extracts was measured using the Folin-Ciocalteu reagent method. Based on the results, Thymus serpyllum extract exhibited a significantly higher antioxidant activity (IC50 = 3.3 \u00b1 0.7) compared to other plant extracts. Accordingly, among the extracts studied, those from Salvia officinalis, Thymus serpyllum, and Origanum vulgare show the most pronounced membrane-stabilizing activity. Antioxidant and antihemolytic properties of green tea and Origanum vulgare extract mixtures were similar to that of each individual plant extract. Similar results were obtained when the green tea extract was mixed with Mentha piperita, Ziziphora bungeana, and Dracocephalum integrifolium extracts, indicating no discernible synergistic interaction.<\/jats:p>","DOI":"10.3390\/plants10040666","type":"journal-article","created":{"date-parts":[[2021,3,31]],"date-time":"2021-03-31T00:13:10Z","timestamp":1617149590000},"page":"666","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Systematic Analysis of Combined Antioxidant and Membrane-Stabilizing Properties of Several Lamiaceae Family Kazakhstani Plants for Potential Production of Tea Beverages"],"prefix":"10.3390","volume":"10","author":[{"given":"Alibek","family":"Ydyrys","sequence":"first","affiliation":[{"name":"Biomedical Research Centre, Al-Farabi Kazakh National University, al-Farabi Av. 71, Almaty 050040, Kazakhstan"},{"name":"Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, al-Farabi Av. 71, Almaty 050040, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nazgul","family":"Zhaparkulova","sequence":"additional","affiliation":[{"name":"Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, al-Farabi Av. 71, Almaty 050040, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arailym","family":"Aralbaeva","sequence":"additional","affiliation":[{"name":"Faculty of Medicine and Health Care, Al-Farabi Kazakh National University, al-Farabi Av. 71, Almaty 050040, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aigul","family":"Mamataeva","sequence":"additional","affiliation":[{"name":"Department of Food Biotechnology, Almaty Technological University, Furkat Str. 348\/4, Almaty 050008, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ainur","family":"Seilkhan","sequence":"additional","affiliation":[{"name":"Faculty of Natural Sciences and Geography, Abai \u041a\u0430zakh National Pedagogical University, Dostuk 13, Almaty 050020, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sayagul","family":"Syraiyl","sequence":"additional","affiliation":[{"name":"Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, al-Farabi Av. 71, Almaty 050040, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ma\u0456ra","family":"Murzakhmetova","sequence":"additional","affiliation":[{"name":"Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, al-Farabi Av. 71, Almaty 050040, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,3,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.ajps.2017.08.004","article-title":"Bioactive flavonoids in medicinal plants: Structure, activity and biological fate","volume":"13","author":"Wang","year":"2018","journal-title":"Asian J. Pharm. Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s10600-020-03184-y","article-title":"Phytochemical Study of Bergenia crassifolia","volume":"56","author":"Akzhigitova","year":"2020","journal-title":"Chem. Nat. Compd."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Koz\u0142owska, A., and Szostak-W\u0119gierek, D. (2017). Flavonoids\u2014Food Sources, Health Benefits, and Mechanisms Involved. Bioactive Molecules in Food, Springer.","DOI":"10.1007\/978-3-319-54528-8_54-1"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1111\/j.1749-6632.2001.tb05818.x","article-title":"Environmental risks and public health","volume":"933","author":"Goldstein","year":"2006","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2483","DOI":"10.1105\/tpc.111.087916","article-title":"Plant Science and Human Nutrition: Challenges in Assessing Health-Promoting Properties of Phytochemicals","volume":"23","author":"Traka","year":"2011","journal-title":"Plant Cell"},{"key":"ref_6","first-page":"934","article-title":"The states of coenpopulations of endemic, relict and rare species of plant Limonium michelsonii and their protection","volume":"26","author":"Ydyrys","year":"2013","journal-title":"World Appl. Sci. J."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/j.ijbiomac.2016.01.090","article-title":"Polyphenolic\u2013polysaccharide conjugates from plants of Rosaceae\/Asteraceae family as potential radioprotectors","volume":"86","author":"Zbikowska","year":"2016","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_8","unstructured":"Watson, R., Preedy, V., and Zibadi, S. (2018). Polyphenols in Human Health and Disease, Academic Press. [2nd ed.]."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Fernandes, I., P\u00e9rez-Gregorio, R., Soares, S., Mateus, N., and De Freitas, V. (2017). Wine Flavonoids in Health and Disease Prevention. Molecules, 22.","DOI":"10.3390\/molecules22020292"},{"key":"ref_10","first-page":"187","article-title":"Natural Flavonoids as Potential Herbal Medication for the Treatment of Diabetes Mellitus and its Complications","volume":"10","author":"Chen","year":"2015","journal-title":"Nat. Prod. Commun."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"3779","DOI":"10.3390\/nu5103779","article-title":"Polyphenols: Benefits to the Cardiovascular System in Health and in Aging","volume":"5","author":"Khurana","year":"2013","journal-title":"Nutrients"},{"key":"ref_12","first-page":"315","article-title":"Free radicals and antioxidants: Physiology, human pathology and therapeutic aspects (part II)","volume":"53","author":"Sahnoun","year":"1998","journal-title":"Therapie"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2014\/761264","article-title":"Oxidative Stress, Prooxidants, and Antioxidants: The Interplay","volume":"2014","author":"Rahal","year":"2014","journal-title":"BioMed Res. Int."},{"key":"ref_14","first-page":"1","article-title":"Oxidative Stress and Lipid Peroxidation Products in Cancer Progression and Therapy","volume":"2012","author":"Barrera","year":"2012","journal-title":"Int. Sch. Res. Not."},{"key":"ref_15","first-page":"1","article-title":"Role of ROS and RNS Sources in Physiological and Pathological Conditions","volume":"2016","author":"Reed","year":"2016","journal-title":"Oxidative Med. Cell. Longev."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"6141","DOI":"10.2174\/1381612811319340008","article-title":"Tea and Health: Studies in Humans","volume":"19","author":"Khan","year":"2013","journal-title":"Curr. Pharm. Des."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.fitote.2014.05.005","article-title":"Role of herbal bioactives as a potential bioavailability enhancer for Active Pharmaceutical Ingredients","volume":"97","author":"Ajazuddin","year":"2014","journal-title":"Fitoterapia"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1573","DOI":"10.1111\/j.1476-5381.2010.00873.x","article-title":"Guidelines for reporting experiments involving animals: The ARRIVE guidelines","volume":"160","author":"McGrath","year":"2010","journal-title":"Br. J. Pharmacol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/0003-2697(79)90738-3","article-title":"Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction","volume":"95","author":"Ohkawa","year":"1979","journal-title":"Anal. Biochem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1007\/s10517-014-2734-3","article-title":"Antitumor Activity of Ethanol Extract from Hippophae Rhamnoides L. Leaves towards Human Acute Myeloid Leukemia Cells In Vitro","volume":"158","author":"Zhamanbaeva","year":"2014","journal-title":"Bull. Exp. Biol. Med."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1016\/S0076-6879(99)99017-1","article-title":"Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent","volume":"299","author":"Singleton","year":"1999","journal-title":"Methods Enzymol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"746","DOI":"10.1002\/ptr.2348","article-title":"Antioxidant and prooxidant properties of a polyphenol-rich extract fromGeranium sanguineum L.in vitro andin vivo","volume":"22","author":"Murzakhmetova","year":"2008","journal-title":"Phytother. Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"3053","DOI":"10.1021\/jf049001r","article-title":"Rapid Tests to Assess the Antioxidant Activity ofPhaseolus vulgarisL. Dry Beans","volume":"53","author":"Heimler","year":"2005","journal-title":"J. Agric. Food Chem."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1129","DOI":"10.1055\/s-0030-1270731","article-title":"Sustainable Medicines and Global Health Care","volume":"77","author":"Cordell","year":"2011","journal-title":"Planta Med."},{"key":"ref_25","first-page":"937","article-title":"The basic science of natural ingredients","volume":"13","author":"Krausz","year":"2014","journal-title":"J. Drugs Derm."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1111\/nyas.13365","article-title":"Protective influence of healthful nutrition on mechanisms of environmental pollutant toxicity and disease risks","volume":"1398","author":"Hoffman","year":"2017","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/S2221-1691(14)60213-6","article-title":"Herbal antioxidant in clinical practice: A review","volume":"4","author":"Alok","year":"2014","journal-title":"Asian Pac. J. Trop. Biomed."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1080\/09637480050029683","article-title":"Antioxidant potential of green and black tea determined using the ferric reducing power (FRAP) assay","volume":"51","year":"2000","journal-title":"Int. J. Food Sci. Nutr."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"910","DOI":"10.1016\/j.ypmed.2004.10.014","article-title":"Antioxidative properties of black tea","volume":"40","author":"Luczaj","year":"2005","journal-title":"Prev. Med."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/07315724.2002.10719187","article-title":"The Role of Tea in Human Health: An Update","volume":"21","author":"McKay","year":"2002","journal-title":"J. Am. Coll. Nutr."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1642S","DOI":"10.3945\/ajcn.113.058230","article-title":"Cellular targets for the beneficial actions of tea polyphenols","volume":"98","author":"Lorenz","year":"2013","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"265","DOI":"10.2174\/1389201016666150118133604","article-title":"Exploring the Nutraceutical Potential of Polyphenols from Black, Green and White Tea Infusions\u2014An Overview","volume":"16","author":"Tenore","year":"2015","journal-title":"Curr. Pharm. Biotechnol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/j.phrs.2011.03.009","article-title":"Tea and cardiovascular disease","volume":"64","author":"Deka","year":"2011","journal-title":"Pharmacol. Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1676S","DOI":"10.3945\/ajcn.113.058271","article-title":"Cancer prevention by green tea: Evidence from epidemiologic studies","volume":"98","author":"Yuan","year":"2013","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.foodchem.2014.02.099","article-title":"Decoction, infusion and hydroalcoholic extract of Origanum vulgare L.: Different performances regarding bioactivity and phenolic compounds","volume":"158","author":"Martins","year":"2014","journal-title":"Food Chem."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1016\/0891-5849(94)00240-K","article-title":"Flavonoids as antioxidant agents: Importance of their interaction with biomembranes","volume":"19","author":"Saija","year":"1995","journal-title":"Free Radic. Biol. Med."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Tungmunnithum, D., Thongboonyou, A., Pholboon, A., and Yangsabai, A. (2018). Flavonoids and Other Phenolic Compounds from Medicinal Plants for Pharmaceutical and Medical Aspects: An Overview. Medicines (Basel), 5.","DOI":"10.3390\/medicines5030093"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"75","DOI":"10.2174\/1871524919666190502105855","article-title":"Antioxidant Effect of Flavonoids Present in Euterpe oleracea Martius and Neurodegenerative Diseases: A Literature Review","volume":"19","author":"Almeida","year":"2019","journal-title":"Cent. Nerv. Syst. Agents Med. Chem."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"\u0160mejkal, K., Malan\u00edk, M., Zhaparkulova, K., Sakipova, Z., Ibragimova, L., Ibadullaeva, G., and \u017demli\u010dka, M. (2016). Kazakh Ziziphora Species as Sources of Bioactive Substances. Molecules, 21.","DOI":"10.3390\/molecules21070826"},{"key":"ref_40","first-page":"266","article-title":"Studies on the Flavonoids of Dracocephalum integrifolium Bge","volume":"22","author":"Guo","year":"1980","journal-title":"J. Integr. Plant Biol."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Bodalska, A., Kowalczyk, A., W\u0142odarczyk, M., and Fecka, I. (2019). Analysis of Polyphenolic Composition of a Herbal Medicinal Product\u2014Peppermint Tincture. Molecules, 25.","DOI":"10.3390\/molecules25010069"},{"key":"ref_42","first-page":"52","article-title":"Salvia Officinalis of different origins antioxidant activity, phenolic and flavonoid content of extracts","volume":"27","author":"Alimpic","year":"2016","journal-title":"Agro Food Ind. Hi Tech."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Marchioni, I., Najar, B., Ruffoni, B., Copetta, A., Pistelli, L., and Pistelli, L. (2020). Bioactive Compounds and Aroma Profile of Some Lamiaceae Edible Flowers. Plants, 9.","DOI":"10.3390\/plants9060691"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1283","DOI":"10.1016\/j.biopha.2017.11.085","article-title":"A composition of medicinal plants with an enhanced ability to suppress microsomal lipid peroxidation and a protective activity against carbon tetrachloride-induced hepatotoxicity","volume":"96","author":"Aralbaeva","year":"2017","journal-title":"Biomed. Pharmacother."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"291","DOI":"10.3109\/10715768809066894","article-title":"Mechanisms of hemolysis induced by copper","volume":"4","author":"Fernandes","year":"1988","journal-title":"Free Radic. Res. Commun."},{"key":"ref_46","first-page":"239","article-title":"(1999) Red blood cell membrane lipid peroxidation and resistance to erythropoietin therapy in hemodialysis patients","volume":"52","author":"Gallucci","year":"1999","journal-title":"Clin. Nephrol."},{"key":"ref_47","first-page":"39","article-title":"The effect of Pycnogenol on the erythrocyte membrane fluidity","volume":"23","author":"Kilanczyk","year":"2004","journal-title":"Gen. Physiol. Biophys."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.fshw.2013.03.004","article-title":"Black tea in chemo-prevention of cancer and other human diseases","volume":"2","author":"Pan","year":"2013","journal-title":"Food Sci. Hum. Wellness"}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/10\/4\/666\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T13:33:12Z","timestamp":1760362392000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/10\/4\/666"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,3,30]]},"references-count":48,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2021,4]]}},"alternative-id":["plants10040666"],"URL":"https:\/\/doi.org\/10.3390\/plants10040666","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,3,30]]}}}