{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T02:41:23Z","timestamp":1783651283080,"version":"3.55.0"},"reference-count":54,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2021,4,12]],"date-time":"2021-04-12T00:00:00Z","timestamp":1618185600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>The aim of this study was to determine the dependence of the total phenolic, flavonoid, and extractive content in extracts of Origanum vulgare L. on the soil pH, humus, total nitrogen (Ntotal), and plant-available\/mobile phosphorus (P2O5), as well as potassium (K2O), total calcium (Ca), magnesium (Mg), and sodium (Na) concentrations. Experimental fields were formed in four locations in Lithuania. Soil and perennial O. vulgare samples were taken at three sites of each experimental field. A total of 12 samples of soil and 12 samples of the tested plant were collected and analyzed. The concentrations of bioactive compounds in plants are significantly (p &lt; 0.05\u20130.01) affected by some chemical properties of the soil: the total phenolic content was reliably correlated with the soil pH, Ntotal, Ca, Mg, and P2O5; the flavonoid content was correlated with the soil P2O5, K2O, Mg, and Na; and the extractive content was correlated with the soil humus, Ntotal, and Na. The obtained results are useful for the development of the commercial cultivation of O. vulgare.<\/jats:p>","DOI":"10.3390\/plants10040750","type":"journal-article","created":{"date-parts":[[2021,4,12]],"date-time":"2021-04-12T11:05:06Z","timestamp":1618225506000},"page":"750","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Dependence of the Concentration of Bioactive Compounds in Origanum vulgare on Chemical Properties of the Soil"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6226-8358","authenticated-orcid":false,"given":"Asta","family":"Klimien\u0117","sequence":"first","affiliation":[{"name":"Botanic Garden, Klaip\u0117da University, H. Manto 84, 92294 Klaip\u0117da, Lithuania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramutis","family":"Klimas","sequence":"additional","affiliation":[{"name":"Department of Medical Technologies, Faculty of Health Sciences, Klaip\u0117da University, H. Manto 84, 92294 Klaip\u0117da, Lithuania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanna","family":"Shutava","sequence":"additional","affiliation":[{"name":"Central Botanical Garden, National Academy of Sciences of Belarus, Surganova 2v, 220012 Minsk, Belarus"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liuda","family":"Razmuvien\u0117","sequence":"additional","affiliation":[{"name":"Botanic Garden, Klaip\u0117da University, H. Manto 84, 92294 Klaip\u0117da, Lithuania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,4,12]]},"reference":[{"key":"ref_1","unstructured":"Natkevi\u010dait\u0117-Ivanauskien\u0117, M. (1976). Lietuvos Respublikos Flora (Flora of the Republic of Lithuania), Mokslas. (In Lithuanian)."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1016\/j.fct.2007.09.106","article-title":"Biological effects of essential oils\u2014A Review","volume":"46","author":"Bakkali","year":"2008","journal-title":"Food Chem. Toxicol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.jep.2004.08.033","article-title":"Polyphenols and antioxidant capacity of bulgarian medicinal plants","volume":"96","author":"Ivanova","year":"2005","journal-title":"J. Ethnopharmacol."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Charles, D.J. (2013). Antioxidant Properties of Spices, Herbs and Other Sources, Springer.","DOI":"10.1007\/978-1-4614-4310-0"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1631\/jzus.B1600377","article-title":"Chemical composition and antioxidant activities of essential oils from different parts of the oregano","volume":"18","author":"Han","year":"2017","journal-title":"J. Zhejiang Univ. Sci. B"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"112271","DOI":"10.1016\/j.indcrop.2020.112271","article-title":"Biomass production, active substance content, and bioaccessibility of greek oregano (Origanum vulgare ssp. hirtum (Link) Ietswaart) following the application of nitrogen","volume":"148","author":"Paszko","year":"2020","journal-title":"Ind. Crops Prod."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"389","DOI":"10.2478\/aoas-2020-0004","article-title":"The applications of origanum vulgare and its derivatives in human, ruminant and fish nutrition\u2014A review","volume":"20","author":"Alagawany","year":"2020","journal-title":"Ann. Anim. Sci."},{"key":"ref_8","first-page":"130","article-title":"Flavonoids and phenolic acids as antioxidants in plants and human health","volume":"16","author":"Saxena","year":"2012","journal-title":"Int. J. Pharmaceut. Sci. Rev. Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1767","DOI":"10.1021\/np100281g","article-title":"Inhibition of melanogenesis and oxidation by protocatechuic acid from Origanum vulgare (oregano)","volume":"73","author":"Chou","year":"2010","journal-title":"J. Nat. Prod."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/j.jep.2004.02.026","article-title":"Anti-hyperglycaemic activity of the aqueous extract of origanum vulgare growing wild in Tafilalet region","volume":"92","author":"Lemhadri","year":"2004","journal-title":"J. Ethnopharmacol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"7690","DOI":"10.1021\/jf302329m","article-title":"Free radical scavenging activity of 4-(3,4-Dihydroxybenzoyloxymethyl) phenyl-O-bD-glucopyranoside from Origanum vulgare and its protection against oxidative damage","volume":"60","author":"Liang","year":"2012","journal-title":"J. Agric. Food Chem."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1016\/j.jfca.2006.09.005","article-title":"Identification and quantification of flavonoids of mexican oregano (Lippia graveolens) by LC-DAD-ESI\/MS analysis","volume":"20","author":"Lin","year":"2007","journal-title":"J. Food Compos. Anal."},{"key":"ref_13","first-page":"609","article-title":"Antibacterial effects of oregano (Origanum vulgare) against gram negative bacilli","volume":"39","author":"Chaudry","year":"2007","journal-title":"Pak. J. Bot."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.plaphy.2013.05.009","article-title":"Plant phenolics: Recent advances on their biosynthesis, genetics and ecophysiology","volume":"72","author":"Cheynier","year":"2013","journal-title":"Plant Physiol. Biochem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"10877","DOI":"10.1021\/jf301807g","article-title":"Biosynthesis, Natural sources, dietary intake, pharmacokinetic properties and biological activities of hydroxycinnamic acids","volume":"60","author":"Khalifa","year":"2012","journal-title":"J. Agric. Food Chem."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1186\/s12986-016-0080-3","article-title":"Hydroxycinnamic acid derivatives: A potential class of natural compounds for the management of lipid metabolism and obesity","volume":"13","author":"Alam","year":"2016","journal-title":"Nutr. Metab."},{"key":"ref_17","unstructured":"Buchanan, B., Gruissem, W., and Jones, R. (2015). Natural products (secondary metabolites). Biochemistry and Molecular Biology of Plants, American Society of Plants."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1006\/anbo.2000.1205","article-title":"Variability of essential oil content and composition of Origanum vulgare L. populations from a North Mediterranean area (Liguria Region, Northern Italy)","volume":"86","author":"Galleti","year":"2000","journal-title":"Ann. Bot. Lond."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1016\/j.foodcont.2011.08.031","article-title":"Evaluation of the antimicrobial and antioxidant activities of essential oils, extracts and their main components from oregano from Madeira Island, Portugal","volume":"23","author":"Castilho","year":"2012","journal-title":"Food Control"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1335","DOI":"10.3389\/fpls.2018.01335","article-title":"Effect of soil pH on the growth, reproductive investment and pollen allergenicity of Ambrosia artemisiifolia L.","volume":"9","author":"Gentili","year":"2018","journal-title":"Front. Plant Sci."},{"key":"ref_21","unstructured":"Motuzas, A., Buivydait\u0117, V., Danilevi\u010dius, V., and \u0160leinys, R. (1996). Dirvotyra (Soil Science). Mokslo ir Enciklopedij\u0173 Leidykla. (In Lithuanian)."},{"key":"ref_22","unstructured":"Staugaitis, G., and Vai\u0161vila, Z.J. (2019). Dirvo\u017eemio Agrocheminiai Tyrimai (Agrochemical Studies), Spalv\u0173 Krait\u0117. (In Lithuanian)."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"11","DOI":"10.2298\/ZMSPN0304011J","article-title":"The availability of base elements (Ca, Mg, Na, K) in some important soil types in serbia","volume":"104","author":"Jakovljevic","year":"2003","journal-title":"Proc. Nat. Sci. Matica Srp."},{"key":"ref_24","first-page":"12","article-title":"Review: Progress of soil enzymology","volume":"48","author":"Wang","year":"2016","journal-title":"Soils"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1016\/j.indcrop.2008.11.001","article-title":"Herbage yield, essential oil content and composition of three oregano (Origanum vulgare L.) populations as affected by soil moisture regimes and nitrogen supply","volume":"29","author":"Azizi","year":"2009","journal-title":"Ind. Crop. Prod."},{"key":"ref_26","first-page":"549","article-title":"Changing environmental conditions and organic fertilizers in Origanum vulgare L.","volume":"6","year":"2015","journal-title":"Front. Plant Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/j.indcrop.2018.03.049","article-title":"Essential oil profile of oregano (Origanum vulgare L.) populations grown under similar soil and climate conditions","volume":"119","author":"Marshedloo","year":"2018","journal-title":"Ind. Crop. Prod."},{"key":"ref_28","unstructured":"Lietuvos Respublikos \u017dem\u0117s \u016akio Ministras (2007). \u017dem\u0117s Na\u0161umo Vertinimo Metodika (Methodology of Land Productivity Assessment), Lietuvos Respublikos \u017dem\u0117s \u016akio Ministras. (In Lithuanian)."},{"key":"ref_29","unstructured":"Ku\u010dinskas, J., Pekarskas, J., Pranskietien\u0117, I., Vai\u0161vila, Z., and \u017demaitis, A. (1999). Agrochemija (Agrochemistry), Lutut\u0117. (In Lithuanian)."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"4452","DOI":"10.1093\/jxb\/eraa049","article-title":"The intersection of nitrogen nutrition and water use in plants: New paths toward improved crops productivity","volume":"71","author":"Plett","year":"2020","journal-title":"J. Exp. Bot."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"558","DOI":"10.17221\/534\/2016-PSE","article-title":"Soil phosphorus and potassium availability in long-term field experiments with organic and mineral fertilization","volume":"62","author":"Muhlbachova","year":"2016","journal-title":"Plant Soil Environ."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/B978-0-12-800132-5.00003-1","article-title":"The physiology of potassium in crop production","volume":"126","author":"Oosterhuis","year":"2014","journal-title":"Adv. Agron."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1073","DOI":"10.1093\/aob\/mcq085","article-title":"Plant nutrition for sustainable development and global health","volume":"105","author":"White","year":"2010","journal-title":"Ann. Bot."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1016\/j.indcrop.2010.11.012","article-title":"Biological activities of extracts of plants grown in Portugal","volume":"33","author":"Albano","year":"2011","journal-title":"Ind. Crops Prod."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"498","DOI":"10.1016\/j.foodcont.2013.03.020","article-title":"Biological activities of the extracts from wild growing Origanum vulgare L.","volume":"33","year":"2013","journal-title":"Food Control"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"W\u0119glarz, Z., Kosakowska, O., Przyby\u0142, J.L., Pi\u00f3ro-Jabrucka, E., and B\u0105czek, K. (2020). The quality of greek oregano (O. vulgare L. subsp. hirtum (Link) Ietswaart) and common oregano (O. vulgare L. subsp. vulgare) cultivated in the temperate climate of Central Europe. Foods, 9.","DOI":"10.3390\/foods9111671"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.bse.2013.03.037","article-title":"Phytochemical diversity of Origanum vulgare L. subsp. vulgare (Lamiaceae) from Austria","volume":"50","author":"Lukas","year":"2013","journal-title":"Biochem. Syst. Ecol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.indcrop.2016.07.038","article-title":"Antioxidant capacity variation in the oregano (Origanum vulgare L.) collection of the german national genebank","volume":"92","author":"Yan","year":"2016","journal-title":"Ind. Crops Prod."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.phytochem.2015.09.008","article-title":"Essential oil diversity of european Origanum vulgare L. (Lamiaceae)","volume":"119","author":"Lukas","year":"2015","journal-title":"Phytochemistry"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1002\/ffj.3197","article-title":"Linalool: A review on a key odorant molecule with valuable biological properties","volume":"29","author":"Aprotosoaie","year":"2014","journal-title":"Flavour Fragr. J."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"3268","DOI":"10.1002\/jssc.200700261","article-title":"Extraction, separation, and detection methods for phenolic acids and flavonoids","volume":"30","author":"Stalikas","year":"2007","journal-title":"J. Sep. Sci."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1186\/s13568-020-01120-5","article-title":"New perspectives of purple starthistle (Centaurea calcitrapa) leaf extracts: Phytochemical analysis, cytotoxicity and antimicrobial activity","volume":"10","year":"2020","journal-title":"AMB Express"},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Santos, S., F\u00e9lix, R., Pais, A., Rocha, S.M., and Silvestre, A. (2019). The quest for phenolic compounds from macroalgae: A review of extraction and identification methodologies. Biomolecules, 9.","DOI":"10.3390\/biom9120847"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"2328","DOI":"10.3390\/molecules18022328","article-title":"Techniques for analysis of plant phenolic compounds","volume":"18","author":"Khoddami","year":"2013","journal-title":"Molecules"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1016\/j.indcrop.2013.01.021","article-title":"Field studies of nitrogen application on greek oregano (Origanum vulgare ssp. hirtum(Link) Ietswaart) essential oil during two cultivation seasons","volume":"46","author":"Karamanos","year":"2013","journal-title":"Ind. Crops Prod."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1080\/01904169909365610","article-title":"Response of wild egyptian oregano to nitrogen fertilization in a sandy soil","volume":"22","author":"Omer","year":"2008","journal-title":"J. Plant Nutr."},{"key":"ref_47","unstructured":"ISO 10390:2005 (2005). Soil Quality\u2014Determination of pH, ISO."},{"key":"ref_48","first-page":"156","article-title":"Methods for soil humus determination","volume":"3","author":"Nikitin","year":"1999","journal-title":"Agro Chem."},{"key":"ref_49","unstructured":"ISO 11261:1995 (1995). Soil Quality\u2014Determination of Total Nitrogen\u2014Modified Kjeldahl Metod, ISO."},{"key":"ref_50","first-page":"199","article-title":"Untersuchungen uber die Chemische Bodenanalyse als Grundlage fur die Beurteilung des Nahrstoffzustandes der Boden, II: Chemische Extractionsmethoden zur Phosphorus\u2014Und Kaliumbestimmung","volume":"26","author":"Egner","year":"1960","journal-title":"K. Lantbr. Ann."},{"key":"ref_51","unstructured":"NFX 31-108:2002 (2002). Soil Quality\u2014Determination of Bases that Can Be Exchanged by Means of Ammonium Acetate\u2014Agitation Method, Association Francaise de Normalisation."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1021\/jf020792b","article-title":"Analysis of antioxidative phenolic compounds in artichoke (Cynara scolymus L.)","volume":"51","author":"Wang","year":"2003","journal-title":"J. Agric. Food Chem."},{"key":"ref_53","first-page":"923462","article-title":"Total flavonoids content in the raw material and aqueous extractives from Bauhinia monandra Kurz (Caesalpiniaceae)","volume":"3","author":"Fernandes","year":"2012","journal-title":"Sci. World J."},{"key":"ref_54","unstructured":"(2011). StatSoft Statistica, StatSoft Inc.. Version 10."}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/10\/4\/750\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T13:26:53Z","timestamp":1760362013000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/10\/4\/750"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,4,12]]},"references-count":54,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2021,4]]}},"alternative-id":["plants10040750"],"URL":"https:\/\/doi.org\/10.3390\/plants10040750","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,4,12]]}}}