{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,22]],"date-time":"2026-03-22T16:08:07Z","timestamp":1774195687758,"version":"3.50.1"},"reference-count":35,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2021,4,16]],"date-time":"2021-04-16T00:00:00Z","timestamp":1618531200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>This study describes the chemical composition, antitumor, antioxidant, and antimicrobial activities of the plant Thymus algeriensis Boiss. Essential oils (EOs) were collected in different periods (before, during, and after flowering stage) from the El-Guetfa region, M\u2019sila, Algeria. The EOs extraction was achieved using three distinguishing techniques: hydro (Clevenger trap), steam, and microwave distillations, targeting different aerial parts of the plant (stems, flowers, and leaves). The EOs chemical components were estimated using GC-FID and GC-MS apparatuses. The resulting yield of the extracted oil was moderate and ranged between 0.84 and 1.53% (wt\/vol). In total, eighty-five components were identified, in which the oxygenated monoterpenes family formed the main portion, starting from 40.56 up to 70.66%. The obtained essential oil was dominated by five major components that varied from low to quite moderate percentages: camphor (17.45\u201332.56%), borneol (11.16\u201322.2%), camphene (7.53\u201312.86%), 1.8-cineole (5.16\u201311.21%), and bornyl acetate (3.86\u20137.92%). The biological results of this oil pointed out that the EOs extracted from the leaves part exposed a weak radical scavenging activity afterward using two well-known antioxidant assays DPPH (IC50 = 8.37 mg\/mL) and ABTS (10.84 mg\/mL). Meanwhile, this oil presented strong inhibition activity against colon cancer cell line HCT116 (LC50 = 39.8 \u00b5g\/mL) and a moderate inhibitory against hepatocellular cancer cells HePG2 (LC50 &gt; 100 \u00b5g\/mL). In addition, this oil antimicrobial activity was quite important against Micrococcus luteus (M. luteus), Staphylococcus aureus CIP 7625, Escherichia coli ATCC 10536, Saccharomyces cerevisiae ATCC 4226, Candida albicans IPA200, Candida tropicalis (Ct), and Candida glabrata (Cg) after using Amoxicillin and Itraconazole as references.<\/jats:p>","DOI":"10.3390\/plants10040786","type":"journal-article","created":{"date-parts":[[2021,4,19]],"date-time":"2021-04-19T06:35:53Z","timestamp":1618814153000},"page":"786","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Biological Properties of Essential Oils from Thymus algeriensis Boiss"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6918-7956","authenticated-orcid":false,"given":"Hamza","family":"Ouakouak","sequence":"first","affiliation":[{"name":"Chemistry Department, University of Hamma Lakhdar, B.P.789, 39000 El-Oued, Algeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0377-0161","authenticated-orcid":false,"given":"Adel","family":"Benarfa","sequence":"additional","affiliation":[{"name":"Technical Platform of Physico-Chemical Analysis (PTAPC-Laghouat-CRAPC), P.O. Box. 37G, Road of Gharda\u00efa, 03000 Laghouat, Algeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3536-6358","authenticated-orcid":false,"given":"Mohammed","family":"Messaoudi","sequence":"additional","affiliation":[{"name":"Chemistry Department, University of Hamma Lakhdar, B.P.789, 39000 El-Oued, Algeria"},{"name":"Nuclear Research Centre of Birine, P.O. Box 180, Ain Oussera, 17200 Djelfa, Algeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1841-2153","authenticated-orcid":false,"given":"Samir","family":"Begaa","sequence":"additional","affiliation":[{"name":"Nuclear Research Centre of Birine, P.O. Box 180, Ain Oussera, 17200 Djelfa, Algeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8183-7624","authenticated-orcid":false,"given":"Barbara","family":"Sawicka","sequence":"additional","affiliation":[{"name":"Department of Plant Production Technology and Commodities Science, University of Life Science in Lublin, Akademicka 15 str., 20-950 Lublin, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5957-0751","authenticated-orcid":false,"given":"Naima","family":"Benchikha","sequence":"additional","affiliation":[{"name":"Chemistry Department, University of Hamma Lakhdar, B.P.789, 39000 El-Oued, Algeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9215-9737","authenticated-orcid":false,"given":"Jesus","family":"Simal-Gandara","sequence":"additional","affiliation":[{"name":"Nutrition and Bromatology Group, Department of Analytical and Food Chemistry, Faculty of Food Science and Technology, University of Vigo\u2014Ourense Campus, E32004 Ourense, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,4,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1016\/j.apjtb.2015.05.007","article-title":"Essential oils used in aromatherapy: A systemic review","volume":"5","author":"Ali","year":"2015","journal-title":"Asian Pac. 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