{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T23:01:19Z","timestamp":1791414079546,"version":"4.3.3"},"reference-count":100,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T00:00:00Z","timestamp":1619395200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Citrus trees face threats from several diseases that affect its production, in particular dry root rot (DRR). DRR is a multifactorial disease mainly attributed to Neocosmospora (Fusarium) solani and other several species of Neocosmospora and Fusarium spp. Nowadays, biological control holds a promising control strategy that showed its great potential as a reliable eco-friendly method for managing DRR disease. In the present study, antagonist rhizobacteria isolates were screened based on in vitro dual culture bioassay with N. solani. Out of 210 bacterial isolates collected from citrus rhizosphere, twenty isolates were selected and identified to the species level based on the 16S rRNA gene. Molecular identification based on 16S rRNA gene revealed nine species belonging to Bacillus, Stenotrophomonas, and Sphingobacterium genus. In addition, their possible mechanisms involved in biocontrol and plant growth promoting traits were also investigated. Results showed that pectinase, cellulose, and chitinase were produced by eighteen, sixteen, and eight bacterial isolates, respectively. All twenty isolates were able to produce amylase and protease, only four isolates produced hydrogen cyanide, fourteen isolates have solubilized tricalcium phosphate, and ten had the ability to produce indole-3-acetic acid (IAA). Surprisingly, antagonist bacteria differed substantially in their ability to produce antimicrobial substances such as bacillomycin (five isolates), iturin (ten isolates), fengycin (six isolates), surfactin (fourteen isolates), and bacteriocin (subtilosin A (six isolates)). Regarding the PGPR capabilities, an increase in the growth of the bacterial treated canola plants, used as a model plant, was observed. Interestingly, both bacterial isolates Bacillus subtilis K4-4 and GH3-8 appear to be more promising as biocontrol agents, since they completely suppressed the disease in greenhouse trials. Moreover, these antagonist bacteria could be used as bio-fertilizer for sustainable agriculture.<\/jats:p>","DOI":"10.3390\/plants10050872","type":"journal-article","created":{"date-parts":[[2021,4,27]],"date-time":"2021-04-27T06:19:11Z","timestamp":1619504351000},"page":"872","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":77,"title":["Potential Role of Rhizobacteria Isolated from Citrus Rhizosphere for Biological Control of Citrus Dry Root Rot"],"prefix":"10.3390","volume":"10","author":[{"given":"Said","family":"Ezrari","sequence":"first","affiliation":[{"name":"Phytopathology Unit, Department of Plant Protection, Ecole Nationale d\u2019Agriculture de Mekn\u00e8s, Km10, Rte Haj Kaddour, BP S\/40, Mekn\u00e8s 50001, Morocco"},{"name":"Laboratory of Functional Ecology and Engineering Environment, Department of Biology, Sidi Mohamed Ben Abdellah University, P.O. Box 2202, Route d\u2019Imouzzer, Fez 30000, Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oumayma","family":"Mhidra","sequence":"additional","affiliation":[{"name":"Phytopathology Unit, Department of Plant Protection, Ecole Nationale d\u2019Agriculture de Mekn\u00e8s, Km10, Rte Haj Kaddour, BP S\/40, Mekn\u00e8s 50001, Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1173-470X","authenticated-orcid":false,"given":"Nabil","family":"Radouane","sequence":"additional","affiliation":[{"name":"Phytopathology Unit, Department of Plant Protection, Ecole Nationale d\u2019Agriculture de Mekn\u00e8s, Km10, Rte Haj Kaddour, BP S\/40, Mekn\u00e8s 50001, Morocco"},{"name":"Laboratory of Functional Ecology and Engineering Environment, Department of Biology, Sidi Mohamed Ben Abdellah University, P.O. Box 2202, Route d\u2019Imouzzer, Fez 30000, Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdessalem","family":"Tahiri","sequence":"additional","affiliation":[{"name":"Phytopathology Unit, Department of Plant Protection, Ecole Nationale d\u2019Agriculture de Mekn\u00e8s, Km10, Rte Haj Kaddour, BP S\/40, Mekn\u00e8s 50001, Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8630-2760","authenticated-orcid":false,"given":"Giancarlo","family":"Polizzi","sequence":"additional","affiliation":[{"name":"Dipartimento di Agricoltura, Alimentazione e Ambiente, sez. Patologia Vegetale, University of Catania, Via S. Sofia 100, 95123 Catania, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abderrahim","family":"Lazraq","sequence":"additional","affiliation":[{"name":"Laboratory of Functional Ecology and Engineering Environment, Department of Biology, Sidi Mohamed Ben Abdellah University, P.O. Box 2202, Route d\u2019Imouzzer, Fez 30000, Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1299-5733","authenticated-orcid":false,"given":"Rachid","family":"Lahlali","sequence":"additional","affiliation":[{"name":"Phytopathology Unit, Department of Plant Protection, Ecole Nationale d\u2019Agriculture de Mekn\u00e8s, Km10, Rte Haj Kaddour, BP S\/40, Mekn\u00e8s 50001, Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,4,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Ezrari, S., Lahlali, R., Radouane, N., Tahiri, A., Asfers, A., Boughalleb\u2011M\u2019Hamdi, N., Amiri, S., and Lazraq, A. (2020). Characterization of Fusarium species causing dry root rot disease of citrus trees in Morocco. J. Plant Dis. Prot.","DOI":"10.1007\/s41348-020-00392-0"},{"key":"ref_2","first-page":"149","article-title":"Key pests and diseases of citrus trees with emphasis on root rot diseases: An overview","volume":"1","author":"Jaouad","year":"2020","journal-title":"Mor. J. Agri. Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3767\/persoonia.2018.40.01","article-title":"Symptomatic citrus trees reveal a new pathogenic lineage in Fusarium and two new Neocosmospora species","volume":"40","author":"Guarnaccia","year":"2018","journal-title":"Persoonia Mol. Phylogeny Evol. Fungi"},{"key":"ref_4","first-page":"127","article-title":"Characterization and pathogenicity of Fusarium solani associated with dry root rot of citrus in the eastern Mediterranean region of Turkey","volume":"20","author":"Kurt","year":"2020","journal-title":"J. Gen. Plant Pathol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1080\/07060661.2019.1633412","article-title":"Grafted twig rot on Citrus sinensis caused by a member of the Fusarium solani species complex","volume":"42","author":"Liu","year":"2019","journal-title":"Can. J. Plant Pathol."},{"key":"ref_6","first-page":"479","article-title":"Association of Fusarium solani with rolABC and wild type troyer citrange","volume":"90","author":"Spina","year":"2008","journal-title":"J. Plant Pathol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/j.simyco.2014.12.002","article-title":"Generic concepts in Nectriaceae","volume":"80","author":"Lombard","year":"2015","journal-title":"Stud. Mycol."},{"key":"ref_8","first-page":"29","article-title":"Current knowledge on Fusarium dry rot of citrus","volume":"2","author":"Adesemoye","year":"2011","journal-title":"Citrograph"},{"key":"ref_9","first-page":"29","article-title":"Interaction between Fusarium solani and Tylenchulus semipenetrans on citrus roots","volume":"21","author":"Labuschagne","year":"1989","journal-title":"Phytophylactica"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"647","DOI":"10.17660\/ActaHortic.2012.940.89","article-title":"Fusarium spp. associated to citrus dry root rot: An emerging issue for Mediterranean citriculture","volume":"940","author":"Yaseen","year":"2012","journal-title":"Acta Hortic."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"81","DOI":"10.36253\/phyto-12183","article-title":"Neocosmospora spp. associated with dry root rot of citrus in South Africa","volume":"60","author":"Guarnaccia","year":"2021","journal-title":"Phytopathol. Mediterr."},{"key":"ref_12","first-page":"86","article-title":"Evaluation of soil amended with bio-agents and compost alone or in combination for controlling citrus nematode Tylenchulus semipenetrans and Fusarium dry root rot on Volkamer lime under greenhouse conditions","volume":"9","author":"Hammam","year":"2016","journal-title":"Int. J. ChemTech Res."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1080\/03235400701453311","article-title":"Efficiency of different application methods of biocontrol agents and biocides in control of Fusarium root rot on some citrus rootstocks","volume":"42","year":"2009","journal-title":"Arch. Phytopathol. Plant Prot."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1191","DOI":"10.1080\/09583157.2018.1520198","article-title":"Impact of delivery method on the efficacy of biological control agents and the virulence of Fusarium root rot pathogens in the greenhouse","volume":"28","author":"Parikh","year":"2018","journal-title":"Biocontrol. Sci. Technol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1016\/j.scienta.2017.10.039","article-title":"Biocontrol of Fusarium mangiferae responsible for mango malformation using bacterial isolates","volume":"230","author":"Veldman","year":"2018","journal-title":"Sci. Hortic."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Vejan, P., Abdullah, R., Khadiran, T., Ismail, S., and Nasrulhaq Boyce, A. (2016). Role of plant growth promoting rhizobacteria in agricultural sustainability-A review. Molecules, 21.","DOI":"10.3390\/molecules21050573"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Dey, R., Pal, K.K., and Tilak, K. (2014). Plant growth promoting rhizobacteria in crop protection and challenges. Future Challenges in Crop Protection against Fungal Pathogens, Springer.","DOI":"10.1007\/978-1-4939-1188-2_2"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Keister, D.L., and Cregan, P.B. (1991). Plant growth promotion mediated by bacterial rhizosphere colonizers. The Rhizosphere and Plant Growth, Springer.","DOI":"10.1007\/978-94-011-3336-4"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.micres.2013.09.009","article-title":"Bacteria with ACC deaminase can promote plant growthand help to feed the world","volume":"169","author":"Glick","year":"2014","journal-title":"Microbiol. Res."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Swarnalakshmi, K., Yadav, V., Tyagi, D., Dhar, D.W., Kannepalli, A., and Kumar, S. (2020). Significance of Plant Growth Promoting Rhizobacteria in Grain Legumes: Growth Promotion and Crop Production. Plants, 9.","DOI":"10.3390\/plants9111596"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Prasad, M., Srinivasan, R., Chaudhary, M., Choudhary, M., and Jat, L.K. (2019). Plant growth promoting rhizobacteria (PGPR) for sustainable agriculture: Perspectives and challenges. PGPR Amelioration in Sustainable Agriculture, Elsevier.","DOI":"10.1016\/B978-0-12-815879-1.00007-0"},{"key":"ref_22","first-page":"1","article-title":"Screening for Fusarium antagonistic bacteria from contrasting niches designated the endophyte bacillus halotoleransas plant warden against fusarium","volume":"10","author":"Slama","year":"2019","journal-title":"Front. Microbiol."},{"key":"ref_23","first-page":"1","article-title":"Plant-growth promotion and biocontrol properties of three Streptomyces spp. isolates to control bacterial rice pathogens","volume":"10","author":"Castellanos","year":"2019","journal-title":"Front. Microbiol."},{"key":"ref_24","first-page":"37","article-title":"Wheat seeds harbour bacterial endophytes with potential as plant growth promoters and biocontrol agents of Fusarium graminearum","volume":"186\u2013187","author":"Grossi","year":"2016","journal-title":"Microbiol. Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1080\/19315260.2016.1271850","article-title":"Biocontrol Potential against Fusarium oxysporum f. sp. lycopersici and Alternaria solani and Tomato Plant Growth Due to Plant Growth\u2013Promoting Rhizobacteria","volume":"23","author":"Paramanandham","year":"2017","journal-title":"Int. J. Veg. Sci."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Singh, M., Singh, D., Gupta, A., Pandey, K.D., Singh, P.K., and Kumar, A. (2019). Plant growth promoting rhizobacteria: Application in biofertilizers and biocontrol of phytopathogens. PGPR Amelioration in Sustainable Agriculture, Elsevier.","DOI":"10.1016\/B978-0-12-815879-1.00003-3"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Verma, M., Mishra, J., and Arora, N.K. (2019). Plant Growth-Promoting Rhizobacteria: Diversity and Applications. Environ. Biotechnol. Sustain. Future, 129\u2013173.","DOI":"10.1007\/978-981-10-7284-0_6"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Basu, A., Prasad, P., Das, S.N., Kalam, S., Sayyed, R.Z., Reddy, M.S., and El Enshasy, H. (2021). Plant Growth Promoting Rhizobacteria (PGPR) as Green Bioinoculants: Recent Developments, Constraints, and Prospects. Sustainability, 13.","DOI":"10.3390\/su13031140"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"616","DOI":"10.3389\/fmicb.2019.00616","article-title":"Biological control agents against Fusarium wilt of banana","volume":"10","author":"Bubici","year":"2019","journal-title":"Front. Microbiol."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Lahlali, R., Mchachti, O., Radouane, N., Ezrari, S., Belabess, Z., Khayi, S., Mentag, R., Tahiri, A., and Barka, E.A. (2020). The Potential of Novel Bacterial Isolates from Natural Soil for the Control of Brown Rot Disease (Monilinia fructigena) on Apple Fruits. Agronomy, 10.","DOI":"10.3390\/agronomy10111814"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.micres.2018.08.001","article-title":"The first report of antifungal lipopeptide production by a Bacillus subtilis subsp. inaquosorum strain","volume":"216","author":"Knight","year":"2018","journal-title":"Microbiol. Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1521","DOI":"10.1007\/s00253-009-2176-4","article-title":"New approach for the detection of non-ribosomal peptide synthetase genes in Bacillus strains by polymerase chain reaction","volume":"85","author":"Tapi","year":"2010","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fmicb.2018.01315","article-title":"Antifungal activity of lipopeptides from Bacillus XT1 CECT 8661 against Botrytis cinerea","volume":"9","author":"Toral","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1425","DOI":"10.2298\/ABS1204425S","article-title":"Screening for the presence of biosynthetic genes for antimicrobial lipopeptides in natural isolates of Bacillus sp.","volume":"64","author":"Fira","year":"2012","journal-title":"Arch. Biol. Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1111\/jam.12214","article-title":"Bacillus amyloliquefaciens ssp. plantarum strains as potential protective starter cultures for the production of Bikalga, an alkaline fermented food","volume":"115","author":"Nielsen","year":"2013","journal-title":"J. Appl. Microbiol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1067","DOI":"10.1111\/j.1365-2672.2007.03626.x","article-title":"Isolation of the Bacillus subtilis antimicrobial peptide subtilosin from the dairy product-derived Bacillus amyloliquefaciens","volume":"104","author":"Sutyak","year":"2008","journal-title":"J. Appl. Microbiol."},{"key":"ref_37","first-page":"123","article-title":"Promoting plant protection by root-associated microbes","volume":"29","author":"Ryu","year":"2013","journal-title":"Plant Pathol. J."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1062","DOI":"10.1080\/13102818.2015.1068135","article-title":"Effects of bacteria-free filtrate from Bacillus megaterium strain L2 on the mycelium growth and spore germination of Alternaria alternata","volume":"29","author":"Li","year":"2015","journal-title":"Biotechnol. Biotechnol. Equip."},{"key":"ref_39","first-page":"1","article-title":"Antagonistic mechanism of iturin a and plipastatin a from Bacillus amyloliquefaciens S76-3 from wheat spikes against Fusarium graminearum","volume":"10","author":"Gong","year":"2015","journal-title":"PLoS ONE"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1016\/j.bcab.2018.10.003","article-title":"A multispecies consortium of bacteria having plant growth promotion and antifungal activities, for the management of Fusarium wilt complex disease in potato (Solanum tuberosum L.)","volume":"16","author":"Devi","year":"2018","journal-title":"Biocatal. Agric. Biotechnol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1007\/s11104-016-3080-1","article-title":"A microbial consortium in the rhizosphere as a new biocontrol approach against Fusarium decline of chickpea","volume":"412","author":"Palmieri","year":"2017","journal-title":"Plant Soil"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s42729-018-0001-9","article-title":"Bacteria Isolated from Treated Wastewater for Biofertilization and Crop Protection Against Fusarium spp. Pathogens","volume":"19","author":"Przemieniecki","year":"2019","journal-title":"J. Soil Sci. Plant Nutr."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1111\/jam.12728","article-title":"Detection and characterization of broad-spectrum antipathogen activity of novel rhizobacterial isolates and suppression of Fusarium crown and root rot disease of tomato","volume":"118","author":"Zhang","year":"2015","journal-title":"J. Appl. Microbiol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.biocontrol.2018.07.002","article-title":"Biocontrol efficiency of native plant growth promoting rhizobacteria against rhizome rot disease of turmeric","volume":"129","author":"Chenniappan","year":"2019","journal-title":"Biol. Control"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fmicb.2018.02363","article-title":"Antifungal activity of bacillus species against Fusarium and analysis of the potential mechanisms used in biocontrol","volume":"9","author":"Khan","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Einloft, T.C., Hartke, S., de Oliveira, P.B., Saraiva, P.S., and Dionello, R.G. (2021). Selection of rhizobacteria for biocontrol of Fusarium verticillioides on non-rhizospheric soil and maize seedlings roots. Eur. J. Plant Pathol., 1\u201316.","DOI":"10.1007\/s10658-021-02259-y"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Ali, M.A., Ren, H., Ahmed, T., Luo, J., An, Q., Qi, X., and Li, B. (2020). Antifungal Effects of Rhizospheric Bacillus Species Against Bayberry Twig Blight Pathogen Pestalotiopsis versicolor. Agronomy, 10.","DOI":"10.3390\/agronomy10111811"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"640","DOI":"10.1016\/j.plaphy.2020.04.016","article-title":"Multi-stress tolerant PGPR Bacillus xiamenensis PM14 activating sugarcane (Saccharum officinarum L.) red rot disease resistance","volume":"151","author":"Amna","year":"2020","journal-title":"Plant Physiol. Biochem."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"594","DOI":"10.1016\/j.ijbiomac.2019.12.216","article-title":"Identification and characterization of a serine protease from Bacillus licheniformis W10: A potential antifungal agent","volume":"145","author":"Ji","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1016\/j.micpath.2017.07.027","article-title":"Isolation and characterization of metabolites from Bacillus licheniformis MH48 with antifungal activity against plant pathogens","volume":"110","author":"Jeong","year":"2017","journal-title":"Microb. Pathog."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Won, S.J., Choub, V., Kwon, J.H., Kim, D.H., and Ahn, Y.S. (2018). The control of fusarium root rot and development of coastal pine (Pinus thunbergii Parl.) Seedlings in a container nursery by use of Bacillus licheniformis MH48. Forests, 10.","DOI":"10.3390\/f10010006"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"928","DOI":"10.1094\/PHYTO-02-17-0051-R","article-title":"Selection and assessment of plant growth-promoting rhizobacteria for biological control of multiple plant diseases","volume":"107","author":"Liu","year":"2017","journal-title":"Phytopathology"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"104016","DOI":"10.1016\/j.biocontrol.2019.104016","article-title":"Postharvest biocontrol of anthracnose in bananas by endophytic and soil rhizosphere bacteria associated with sisal (Agave sisalana) in Brazil","volume":"137","author":"Damasceno","year":"2019","journal-title":"Biol. Control"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.bcab.2017.11.007","article-title":"Pseudomonas stutzeri E25 and Stenotrophomonas maltophilia CR71 endophytes produce antifungal volatile organic compounds and exhibit additive plant growth-promoting effects","volume":"13","author":"Santoyo","year":"2018","journal-title":"Biocatal. Agric. Biotechnol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"101555","DOI":"10.1016\/j.pmpp.2020.101555","article-title":"Volatile compounds mediated effects of Stenotrophomonas maltophilia strain UN1512 in plant growth promotion and its potential for the biocontrol of Colletotrichum nymphaeae","volume":"112","author":"Alijani","year":"2020","journal-title":"Physiol. Mol. Plant Pathol."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Sahu, K.P., Kumar, A., Patel, A., Kumar, M., Gopalakrishnan, S., Prakash, G., Rathour, R., and Gogoi, R. (2020). Rice Blast Lesions: An Unexplored Phyllosphere Microhabitat for Novel Antagonistic Bacterial Species Against Magnaporthe oryzae. Microb. Ecol.","DOI":"10.1007\/s00248-020-01617-3"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"137171","DOI":"10.1016\/j.scitotenv.2020.137171","article-title":"Characterization of hexaconazole-degrading strain Sphingobacterium multivorum and analysis of transcriptome for biodegradation mechanism","volume":"722","author":"An","year":"2020","journal-title":"Sci. Total Environ."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/j.biocontrol.2019.01.004","article-title":"Use of biocontrol agents as potential tools in the management of chestnut blight","volume":"132","author":"Murolo","year":"2019","journal-title":"Biol. Control"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.micres.2018.11.009","article-title":"Avocado rhizobacteria emit volatile organic compounds with antifungal activity against Fusarium solani, Fusarium sp. associated with Kuroshio shot hole borer, and Colletotrichum gloeosporioides","volume":"219","author":"Eskalen","year":"2019","journal-title":"Microbiol. Res."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.biocontrol.2012.06.008","article-title":"Improvement of peanut rhizobial inoculant by incorporation of plant growth promoting rhizobacteria (PGPR) as biocontrol against the seed borne fungus, Aspergillus niger","volume":"63","author":"Yuttavanichakul","year":"2012","journal-title":"Biol. Control"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1007\/s12223-019-00764-6","article-title":"Evaluation of antagonistic activity and mechanisms of endophytic yeasts against pathogenic fungi causing economic crop diseases","volume":"65","author":"Khunnamwong","year":"2020","journal-title":"Folia Microbiol."},{"key":"ref_62","first-page":"1","article-title":"Microbial volatiles: Small molecules with an important role in intra- and inter-kingdom interactions","volume":"8","author":"Garbeva","year":"2017","journal-title":"Front. Microbiol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1094\/MPMI.2003.16.6.525","article-title":"Phylogeny of HCN Synthase-Encoding hcnBC Genes in Biocontrol Fluorescent Pseudomonads and Its Relationship with Host Plant Species and HCN Synthesis Ability","volume":"16","author":"Ramette","year":"2003","journal-title":"Mol. Plant-Microbe Interact."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1007\/s002039900127","article-title":"Mechanism, regulation, and ecological role of bacterial cyanide biosynthesis","volume":"173","author":"Blumer","year":"2000","journal-title":"Arch. Microbiol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1007\/s10658-011-9789-z","article-title":"Multifarious activity of bioformulated Pseudomonas fluorescens PS1 and biocontrol of Sclerotinia sclerotiorum in Indian rapeseed (Brassica campestris L.)","volume":"131","author":"Aeron","year":"2011","journal-title":"Eur. J. Plant Pathol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1016\/j.bcab.2018.06.019","article-title":"Inoculation of siderophore producing rhizobacteria and their consortium for growth enhancement of wheat plant","volume":"15","author":"Kumar","year":"2018","journal-title":"Biocatal. Agric. Biotechnol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1590\/S1415-475738420150053","article-title":"Plant growth-promoting bacteria as inoculants in agricultural soils","volume":"38","author":"Souza","year":"2015","journal-title":"Genet. Mol. Biol."},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Lebrazi, S., Niehaus, K., Bednarz, H., Fadil, M., Chraibi, M., and Fikri-Benbrahim, K. (2020). Screening and optimization of indole-3-acetic acid production and phosphate solubilization by rhizobacterial strains isolated from Acacia cyanophylla root nodules and their effects on its plant growth. J. Genet. Eng. Biotechnol., 18.","DOI":"10.1186\/s43141-020-00090-2"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.biocontrol.2016.10.008","article-title":"Identification and biocontrol potential of antagonistic bacteria strains against Sclerotinia sclerotiorum and their growth-promoting effects on Brassica napus","volume":"104","author":"Sun","year":"2017","journal-title":"Biol. Control"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fmicb.2015.00198","article-title":"Isolation and characterization of plant growth-promoting rhizobacteria from wheat rhizosphere and their effect on plant growth promotion","volume":"6","author":"Majeed","year":"2015","journal-title":"Front. Microbiol."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1016\/j.micres.2012.05.002","article-title":"Bacillus strains isolated from rhizosphere showed plant growth promoting and antagonistic activity against phytopathogens","volume":"167","author":"Kumar","year":"2012","journal-title":"Microbiol. Res."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s42452-019-1149-1","article-title":"Potential biocontrol and superlative plant growth promoting activity of indigenous Bacillus mojavensis PB\u201135 (R11) of soybean (Glycine max) rhizosphere","volume":"1","author":"Prajakta","year":"2019","journal-title":"SN Appl. Sci."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"488","DOI":"10.5423\/PPJ.OA.04.2017.0073","article-title":"Diffusible and volatile antifungal compounds produced by an antagonistic Bacillus velezensis G341 against various phytopathogenic fungi","volume":"33","author":"Lim","year":"2017","journal-title":"Plant Pathol. J."},{"key":"ref_74","doi-asserted-by":"crossref","unstructured":"Mart\u00ednez-Raudales, I., De La Cruz-Rodr\u00edguez, Y., Vega-Arregu\u00edn, J., Alvarado-Guti\u00e9rrez, A., Fraire-Mayorga, A., Alvarado-Rodr\u00edguez, M., Balderas-Hern\u00e1ndez, V., G\u00f3mez-Soto, J.M., and Fraire-Vel\u00e1zquez, S. (2017). Draft Genome Sequence of Bacillus velezensis 3A-25B, a Strain with Biocontrol Activity against Fungal and Oomycete Root Plant Phytopathogens, Isolated from Grassland Soil. Genome Announc., 5.","DOI":"10.1128\/genomeA.01021-17"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.jbiotec.2018.07.044","article-title":"Biological control of plant pathogens by Bacillus species","volume":"285","author":"Fira","year":"2018","journal-title":"J. Biotechnol."},{"key":"ref_76","first-page":"1","article-title":"The profile and antimicrobial activity of Bacillus lipopeptide extracts of five potential biocontrol strains","volume":"8","author":"Dimkic","year":"2017","journal-title":"Front. Microbiol."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1007\/s12223-011-0056-7","article-title":"Production of lipopeptides among Bacillus strains showing growth inhibition of phytopathogenic fungi","volume":"56","author":"Velho","year":"2011","journal-title":"Folia Microbiol."},{"key":"ref_78","first-page":"1","article-title":"Antagonism of Two Plant-Growth Promoting Bacillus velezensis Isolates Against Ralstonia solanacearum and Fusarium oxysporum","volume":"8","author":"Cao","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"e00742","DOI":"10.1002\/mbo3.742","article-title":"Selecting lipopeptide-producing, Fusarium-suppressing Bacillus spp.: Metabolomic and genomic probing of Bacillus velezensis NWUMFkBS10.5","volume":"8","author":"Adeniji","year":"2019","journal-title":"Microbiologyopen"},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.micres.2016.06.002","article-title":"Bacillus velezensis RC 218 as a biocontrol agent to reduce Fusarium head blight and deoxynivalenol accumulation: Genome sequencing and secondary metabolite cluster profiles","volume":"192","author":"Palazzini","year":"2016","journal-title":"Microbiol. Res."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/j.micres.2016.06.012","article-title":"Antagonist effects of Bacillus spp. strains against Fusarium graminearum for protection of durum wheat (Triticum turgidum L. subsp. durum)","volume":"192","author":"Ali","year":"2016","journal-title":"Microbiol. Res."},{"key":"ref_82","doi-asserted-by":"crossref","unstructured":"Khan, N., Maymon, M., and Hirsch, A. (2017). Combating Fusarium Infection Using Bacillus-Based Antimicrobials. Microorganisms, 5.","DOI":"10.3390\/microorganisms5040075"},{"key":"ref_83","doi-asserted-by":"crossref","unstructured":"Syed-Ab-Rahman, S.F., Carvalhais, L.C., Chua, E., Xiao, Y., Wass, T.J., and Schenk, P.M. (2018). Identification of soil bacterial isolates suppressing different Phytophthora spp. And promoting plant growth. Front. Plant Sci., 871.","DOI":"10.3389\/fpls.2018.01502"},{"key":"ref_84","unstructured":"Win, T.T., Bo, B., Malec, P., and Fu, P. (2021). The effect of a consortium of Penicillium sp. and Bacillus sp. in suppressing banana fungal diseases caused by Fusarium sp. and Alternaria sp.. J. Appl. Microbiol., jam.15067."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1007\/s12600-018-0706-2","article-title":"Stem inoculation with bacterial strains Bacillus amyloliquefaciens (GB03) and Microbacterium imperiale (MAIIF2a) mitigates Fusarium root rot in cassava","volume":"47","author":"Freitas","year":"2019","journal-title":"Phytoparasitica"},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1080\/03235408.2018.1441098","article-title":"Relationship of in vitro and in planta screening: Improving the selection process for biological control agents against Fusarium root rot in row crops","volume":"51","author":"Parikh","year":"2018","journal-title":"Arch. Phytopathol. Plant Prot."},{"key":"ref_87","first-page":"477","article-title":"Protective treatments against soilborne pathogens in citrus orchards","volume":"50","author":"Mohamed","year":"2010","journal-title":"J. Plant Prot. Res."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.apsoil.2017.10.025","article-title":"Anti-fungal activity of bacterial endophytes associated with legumes against Fusarium solani: Assessment of fungi soil suppressiveness and plant protection induction","volume":"124","author":"Bahroun","year":"2018","journal-title":"Appl. Soil Ecol."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fmicb.2015.01360","article-title":"Isolation and Identification of Plant Growth Promoting Rhizobacteria from Cucumber Rhizosphere and Their Effect on Plant Growth Promotion and Disease Suppression","volume":"6","author":"Islam","year":"2016","journal-title":"Front. Microbiol."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"2071","DOI":"10.1128\/AEM.66.5.2071-2078.2000","article-title":"Development of a highly sensitive nested-PCR procedure using a single closed tube for detection of Erwinia amylovora in asymptomatic plant material","volume":"66","author":"Llop","year":"2000","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1128\/jb.173.2.697-703.1991","article-title":"16S ribosomal DNA amplification for phylogenetic study","volume":"173","author":"Weisburg","year":"1991","journal-title":"J. Bacteriol."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1007\/s10482-017-0977-5","article-title":"Antifungal activity of avocado rhizobacteria against Fusarium euwallaceae and Graphium spp., associated with Euwallacea spp. nr. fornicatus, and Phytophthora cinnamomi","volume":"111","author":"Carrillo","year":"2018","journal-title":"Antonie van Leeuwenhoek Int. J. Gen. Mol. Microbiol."},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.micres.2015.01.014","article-title":"Isolation, characterization, and evaluation of multi-trait plant growth promoting rhizobacteria for their growth promoting and disease suppressing effects on ginger","volume":"173","author":"Dinesh","year":"2015","journal-title":"Microbiol. Res."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"560","DOI":"10.1080\/03235408.2018.1557884","article-title":"Evaluation of halotolerant endophytic bacteria isolated from the halophyte suaeda for biological control of fungal rice pathogens","volume":"52","author":"Etesami","year":"2019","journal-title":"Arch. Phytopathol. Plant Prot."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1128\/am.29.3.422-426.1975","article-title":"Powdered Chitin Agar as a Selective Medium for Enumeration of Actinomycetes in Water and Soil1","volume":"29","author":"Hsu","year":"1975","journal-title":"Appl. Microbiol."},{"key":"ref_96","first-page":"206","article-title":"Chitinolytic activity of Enterobacter sp. KB3 antagonistic to Rhizoctonia solani and its role in the degradation of living fungal hyphae","volume":"2","author":"Velusamy","year":"2011","journal-title":"Int. Res. J. Microbiol."},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/0003-2697(87)90612-9","article-title":"Universal chemical assay for the detection and determination of siderophores","volume":"160","author":"Schwyn","year":"1987","journal-title":"Anal. Biochem."},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/j.femsec.2004.11.017","article-title":"Effects of the humic substances of de-inking paper sludge on the antagonism between two compost bacteria and Pythium ultimum","volume":"52","author":"Charest","year":"2005","journal-title":"FEMS Microbiol. Ecol."},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1080\/15226514.2013.773283","article-title":"Bin Characterization of Bacteria in the Rhizosphere Soils of Polygonum Pubescens and Their Potential in Promoting Growth and Cd, Pb, Zn Uptake by Brassica napus","volume":"16","author":"Jing","year":"2014","journal-title":"Int. J. Phytoremediation"},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"1171","DOI":"10.1016\/j.chemosphere.2012.02.036","article-title":"Assessment of bacterial communities and characterization of lead-resistant bacteria in the rhizosphere soils of metal-tolerant Chenopodium ambrosioides grown on lead-zinc mine tailings","volume":"87","author":"Zhang","year":"2012","journal-title":"Chemosphere"}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/10\/5\/872\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T05:53:01Z","timestamp":1760161981000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/10\/5\/872"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,4,26]]},"references-count":100,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2021,5]]}},"alternative-id":["plants10050872"],"URL":"https:\/\/doi.org\/10.3390\/plants10050872","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,4,26]]}}}