{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T05:02:33Z","timestamp":1787029353094,"version":"build-2736575974"},"reference-count":62,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2021,5,27]],"date-time":"2021-05-27T00:00:00Z","timestamp":1622073600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>The anti-inflammatory, thrombolytic, and hair growth-promoting activity of the n-hexane fraction from the methanol extract of Leea indica (NFLI) leaves was investigated. NFLI showed significant inhibition of hemolysis and protein denaturation, and exhibited a concentration-dependent thrombolytic activity. When applied topically to mice at concentrations of 10, 1, 0.1%, NFLI demonstrated a significant increase in average hair length (p &lt; 0.001) compared with untreated animals. NFLI (1% concentration) exhibited the highest percentage of hair regrowth on day 7, 14 and 21 (81.24, 65.60, and 62.5%, respectively). An in silico study was further conducted to predict the binding affinity of phytochemicals previously reported in L. indica towards PGD2 synthase (PDB ID: 2VD1), an enzyme that catalyses the isomerisation of prostaglandin H2 to PGD2 which is involved in hair loss. Phthalic acid, farnesol, n-tricosane, n-tetracosane, and n-heptacosane showed the best ligand efficiencies towards PGD2 synthase and their intermolecular interactions were visualised using BIOVIA Discovery Studio Visualizer. Our results indicate that L. indica could represent a promising natural alternative to tackle alopecia.<\/jats:p>","DOI":"10.3390\/plants10061081","type":"journal-article","created":{"date-parts":[[2021,5,27]],"date-time":"2021-05-27T23:16:46Z","timestamp":1622157406000},"page":"1081","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Anti-Inflammatory, Thrombolytic and Hair-Growth Promoting Activity of the n-Hexane Fraction of the Methanol Extract of Leea indica Leaves"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3828-0768","authenticated-orcid":false,"given":"Shahenur Alam","family":"Sakib","sequence":"first","affiliation":[{"name":"Department of Pharmacy, International Islamic University Chittagong, Kumira, Chittagong 4318, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2704-7610","authenticated-orcid":false,"given":"Abu Montakim","family":"Tareq","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, International Islamic University Chittagong, Kumira, Chittagong 4318, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ameerul","family":"Islam","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, International Islamic University Chittagong, Kumira, Chittagong 4318, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3335-0368","authenticated-orcid":false,"given":"Ahmed","family":"Rakib","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Faculty of Biological Sciences, University of Chittagong, Chittagong 4331, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4021-106X","authenticated-orcid":false,"given":"Mohammad Nazmul","family":"Islam","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, International Islamic University Chittagong, Kumira, Chittagong 4318, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad Arafat","family":"Uddin","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, International Islamic University Chittagong, Kumira, Chittagong 4318, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2065-9630","authenticated-orcid":false,"given":"Md. Masudur","family":"Rahman","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, International Islamic University Chittagong, Kumira, Chittagong 4318, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3880-5261","authenticated-orcid":false,"given":"Veronique","family":"Seidel","sequence":"additional","affiliation":[{"name":"Natural Products Research Laboratory, Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow G4 0RE, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3188-2272","authenticated-orcid":false,"given":"Talha Bin","family":"Emran","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, BGC Trust University Bangladesh, Chittagong 4381, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,5,27]]},"reference":[{"key":"ref_1","unstructured":"Burkill, I.H. (1966). A Dictionary of the Economic Products of the Malay Peninsula, Ministry of Agriculture and Co-Operatices. [2nd ed.]."},{"key":"ref_2","first-page":"101","article-title":"A multi-variate approach to the study of medicinal plants in Malaysia","volume":"13","author":"Lattif","year":"1984","journal-title":"J. Singap. Natl. Acad. Sci."},{"key":"ref_3","unstructured":"Chatterjee, A., and Pakrashi, S.C. (1991). Treatise on Indian Medicinal Plants, Publications & Information Directorate."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/0378-8741(92)90033-N","article-title":"Maternity and medicinal plants in Vanuatu. I. The cycle of reproduction","volume":"37","author":"Bourdy","year":"1992","journal-title":"J. Ethnopharmacol."},{"key":"ref_5","unstructured":"Prajapati, N.D., Purohit, S.S., Sharma, A.K., and Kumar, T. (2003). A Handbook of Medicinal Plants: A Complete Source Book, Agrobios."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Khare, C.P. (2008). Indian Medicinal Plants: An Illustrated Dictionary, Springer Science & Business Media.","DOI":"10.1007\/978-0-387-70638-2"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"207","DOI":"10.2478\/v10007-008-0002-7","article-title":"Identification of chemical compounds from the leaves of Leea indica","volume":"58","author":"Srinivasan","year":"2008","journal-title":"Acta Pharm."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Singh, D., Siew, Y.-Y., Chong, T.-I., Yew, H.-C., Ho, S.S., Lim, C.S., Tan, W.-X., Neo, S.-Y., and Koh, H.-L. (2019). Identification of Phytoconstituents in Leea indica (Burm. F.) Merr. Leaves by High Performance Liquid Chromatography Micro Time-of-Flight Mass Spectrometry. Molecules, 24.","DOI":"10.3390\/molecules24040714"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"164689","DOI":"10.1155\/2012\/164689","article-title":"Bioassay-Guided Isolation of Cytotoxic Cycloartane Triterpenoid Glycosides from the Traditionally Used Medicinal Plant Leea indica","volume":"2012","author":"Wong","year":"2012","journal-title":"Evid. Based Complement Altern. Med."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"185","DOI":"10.5582\/ddt.2011.v5.4.185","article-title":"Sedative and anxiolytic effects of the methanolic extract of Leea indica (Burm. f.) Merr. leaf","volume":"5","author":"Raihan","year":"2011","journal-title":"Drug Discov."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"8954","DOI":"10.1038\/s41598-020-65728-0","article-title":"PLGA nanoparticles loaded with Gallic acid- a constituent of Leea indica against Acanthamoeba triangularis","volume":"10","author":"Mahboob","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"14651","DOI":"10.3390\/molecules181214651","article-title":"Anti-and pro-lipase activity of selected medicinal, herbal and aquatic plants, and structure elucidation of an anti-lipase compound","volume":"18","author":"Ado","year":"2013","journal-title":"Molecules"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/j.jep.2004.03.007","article-title":"Evaluation of antioxidant and nitric oxide inhibitory activities of selected Malaysian medicinal plants","volume":"92","author":"Saha","year":"2004","journal-title":"J. Ethnopharmacol."},{"key":"ref_14","first-page":"77","article-title":"Anti-proliferative activities of 32 Malaysian plant species in breast cancer cell lines","volume":"20","author":"Nurhanan","year":"2008","journal-title":"J. Trop. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Rahman, M.A., Sultana, R., Bin Emran, T., Islam, M.S., Rahman, M.A., Chakma, J.S., Rashid, H.-u., and Hasan, C.M.M. (2013). Effects of organic extracts of six Bangladeshi plants on in vitro thrombolysis and cytotoxicity. BMC Complement Altern. Med., 13.","DOI":"10.1186\/1472-6882-13-25"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Reddy, N.S., Navanesan, S., Sinniah, S.K., Wahab, N.A., and Sim, K.S. (2012). Phenolic content, antioxidant effect and cytotoxic activity of Leea indica leaves. BMC Complement Altern. Med., 12.","DOI":"10.1186\/1472-6882-12-128"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"293060","DOI":"10.1155\/2011\/293060","article-title":"Leea indica Ethyl Acetate Fraction Induces Growth-Inhibitory Effect in Various Cancer Cell Lines and Apoptosis in Ca Ski Human Cervical Epidermoid Carcinoma Cells","volume":"2011","author":"Wong","year":"2011","journal-title":"Evid. Based Complement Altern. Med."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"684740","DOI":"10.1155\/2012\/684740","article-title":"Induction of Mitochondria-Mediated Apoptosis in Ca Ski Human Cervical Cancer Cells Triggered by Mollic Acid Arabinoside Isolated from Leea indica","volume":"2012","author":"Wong","year":"2012","journal-title":"Evid. Based Complement Altern. Med."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.imr.2017.01.004","article-title":"In vitro antioxidant and anticancer activity of Leea indica leaf extracts on human prostate cancer cell lines","volume":"6","author":"Ghagane","year":"2017","journal-title":"Integr. Med. Res."},{"key":"ref_20","first-page":"S71","article-title":"Traditional uses, chemistry and pharmacological activities of Leea indica (Burm. f.) Merr. (Vitaceae): A comprehensive review","volume":"12","author":"Kekuda","year":"2018","journal-title":"Int. J. Green Pharm."},{"key":"ref_21","first-page":"3155","article-title":"Anti-hyperglycemic and hypolipidemic activity of Leea indica","volume":"3","author":"Dalu","year":"2014","journal-title":"Int. J. Bioassay"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.jep.2018.12.040","article-title":"Evaluation of anti-proliferative activity of medicinal plants used in Asian Traditional Medicine to treat cancer","volume":"235","author":"Siew","year":"2019","journal-title":"J. Ethnopharmacol."},{"key":"ref_23","first-page":"27","article-title":"Comparative anti-dirrhoeal and antimicrobial activities of methanol extract of Leea indica (Burm. F.) Merr. and Leea macrophylla Roxb. Ex. Hornem (Fam. Vitaceae) and four Bangladeshi market preparations","volume":"9","author":"Tareq","year":"2017","journal-title":"Der. Pharma. Chem."},{"key":"ref_24","first-page":"114","article-title":"Effect of single and combinational herbal formulation in alloxan induced hyperglycemia","volume":"8","author":"Patel","year":"2016","journal-title":"Der. Pharm. Lett."},{"key":"ref_25","first-page":"163","article-title":"Antioxidant and antimalarial activity of Leea indica leaf extract against malaria-mice model","volume":"7","author":"Sulistyaningsih","year":"2017","journal-title":"J. Appl. Pharm. Sci."},{"key":"ref_26","first-page":"6","article-title":"Larvicidal and repellent efficacy of some of the weed plant extracts against Culex quinquefasciatus Say","volume":"8","author":"Sreedhanya","year":"2017","journal-title":"J. Adv. Lab. Res. Biol."},{"key":"ref_27","first-page":"150","article-title":"Analgesic activity of Leea indica (Burm.f.) Merr","volume":"3","author":"Emran","year":"2012","journal-title":"Phytopharmacology"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"470","DOI":"10.1016\/j.jep.2015.10.005","article-title":"In silico prediction of prostaglandin D2 synthase inhibitors from herbal constituents for the treatment of hair loss","volume":"175","author":"Fong","year":"2015","journal-title":"J. Ethnopharmacol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1016\/j.nut.2004.09.016","article-title":"Testing various herbs for antithrombotic effect","volume":"21","author":"Yamamoto","year":"2005","journal-title":"Nutrition"},{"key":"ref_30","first-page":"1","article-title":"Hair Growth Promoting Effect of Dicerocaryum senecioides Phytochemicals","volume":"2019","author":"Rambwawasvika","year":"2019","journal-title":"Int. J. Med. Chem."},{"key":"ref_31","unstructured":"Blume-Peytavi, U., Whiting, D.A., and Tr\u00fceb, R.M. (2008). Hair Growth and Disorders, Springer Science & Business Media."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1179","DOI":"10.1111\/brv.12151","article-title":"Resting no more: Re-defining telogen, the maintenance stage of the hair-growth cycle","volume":"90","author":"Geyfman","year":"2015","journal-title":"Biol. Rev."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"847","DOI":"10.1038\/nm.3643","article-title":"Emerging interactions between skin stem cells and their niches","volume":"20","author":"Hsu","year":"2014","journal-title":"Nat. Med."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"103","DOI":"10.5487\/TR.2016.32.2.103","article-title":"Hair growth-promoting effects of lavender oil in C57BL\/6 mice","volume":"32","author":"Lee","year":"2016","journal-title":"Toxicol. Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1394","DOI":"10.1016\/j.ejmech.2005.03.012","article-title":"2-Arylidene-4-(4-phenoxy-phenyl)but-3-en-4-olides: Synthesis, reactions and biological activity","volume":"40","author":"Husain","year":"2005","journal-title":"Eur. J. Med. Chem."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"53","DOI":"10.2165\/00003495-200161010-00006","article-title":"Treatments for androgenetic alopecia and alopecia areata","volume":"61","author":"Meidan","year":"2001","journal-title":"Drugs"},{"key":"ref_37","first-page":"98","article-title":"What\u2019s new in androgenetic alopecia: Approvals, long-term safety data, cancer risk and treatment options for women","volume":"10","author":"Bergstrom","year":"2011","journal-title":"J. Drugs Derm."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1007\/s40257-014-0077-5","article-title":"Androgenetic alopecia: An evidence-based treatment update","volume":"15","author":"Varothai","year":"2014","journal-title":"Am. J. Clin. Derm."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"2777","DOI":"10.2147\/DDDT.S214907","article-title":"Minoxidil and its use in hair disorders: A review","volume":"13","author":"Suchonwanit","year":"2019","journal-title":"Drug Des. Devel."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"130","DOI":"10.2174\/187221312800166859","article-title":"Minoxidil use in dermatology, side effects and recent patents","volume":"6","author":"Rossi","year":"2012","journal-title":"Recent Pat Inflamm. Allergy Drug Discov."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1016\/j.jaad.2020.06.1009","article-title":"Oral minoxidil treatment for hair loss: A review of efficacy and safety","volume":"84","author":"Randolph","year":"2020","journal-title":"J. Am. Acad. Derm."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"126ra34","DOI":"10.1126\/scitranslmed.3003122","article-title":"Prostaglandin D2 Inhibits Hair Growth and Is Elevated in Bald Scalp of Men with Androgenetic Alopecia","volume":"4","author":"Garza","year":"2012","journal-title":"Sci. Transl. Med."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1111\/exd.12348","article-title":"Does prostaglandin D2 hold the cure to male pattern baldness?","volume":"23","author":"Nieves","year":"2014","journal-title":"Exp. Derm."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"2178","DOI":"10.1021\/jm701509k","article-title":"Novel Prostaglandin D Synthase Inhibitors Generated by Fragment-Based Drug Design","volume":"51","author":"Hohwy","year":"2008","journal-title":"J. Med. Chem."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1085","DOI":"10.1016\/S0092-8674(00)80374-8","article-title":"Cloning and crystal structure of hematopoietic prostaglandin D synthase","volume":"90","author":"Kanaoka","year":"1997","journal-title":"Cell"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"4726","DOI":"10.1016\/j.bmc.2018.08.014","article-title":"Characterization of crystal water molecules in a high-affinity inhibitor and hematopoietic prostaglandin D synthase complex by interaction energy studies","volume":"26","author":"Takaya","year":"2018","journal-title":"Bioorg. Med. Chem."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"191","DOI":"10.5487\/TR.2011.27.4.191","article-title":"Toxicological characterization of phthalic acid","volume":"27","author":"Lee","year":"2011","journal-title":"Toxicol. Res."},{"key":"ref_48","first-page":"2938","article-title":"Antioxidant, cytotoxic and phytochemical properties of the ethanol extract of Leea indica leaf","volume":"5","author":"Emran","year":"2012","journal-title":"J. Pharm. Res."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/S0923-1811(02)00146-9","article-title":"The extract of Thujae occidentalis semen inhibited 5\u03b1-reductase and androchronogenetic alopecia of B6CBAF1\/j hybrid mouse","volume":"31","author":"Park","year":"2003","journal-title":"J. Derm. Sci."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"3090","DOI":"10.2174\/1381612824666180911114810","article-title":"Medicinal Plants for the Treatment of Hair Loss and the Suggested Mechanisms","volume":"24","author":"Shen","year":"2018","journal-title":"Curr. Pharm. Des."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"966","DOI":"10.1111\/jocd.12930","article-title":"An overview of herbal alternatives in androgenetic alopecia","volume":"18","author":"Dhariwala","year":"2019","journal-title":"J. Cosmet. Derm."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Emran, T.B., Rahman, M.A., Uddin, M.M.N., Rahman, M.M., Uddin, M.Z., Dash, R., and Layzu, C. (2015). Effects of organic extracts and their different fractions of five Bangladeshi plants on in vitro thrombolysis. BMC Complement Altern. Med., 15.","DOI":"10.1186\/s12906-015-0643-2"},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Babar, Z.M., Jaswir, I., Tareq, A.M., Ali Reza, A.S.M., Azizi, W.M., Hafidz, M., Ahfter, F., Hasan, M., Farhad, S., and Uddin, M.M.R. (2019). In vivo anxiolytic and in vitro anti-inflammatory activities of water-soluble extract (WSE) of Nigella sativa (L.) seeds. Nat. Prod. Res., 1\u20136.","DOI":"10.1080\/14786419.2019.1667348"},{"key":"ref_54","first-page":"51","article-title":"Anti-inflammatory, anti-diarrheal, thrombolytic and cytotoxic activities of an ornamental medicinal plant: Persicaria orientalis","volume":"28","author":"Uddin","year":"2016","journal-title":"J. Basic Clin. Physiol. Pharm."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1186\/1477-9560-4-14","article-title":"Development of an in vitro model to study clot lysis activity of thrombolytic drugs","volume":"4","author":"Prasad","year":"2006","journal-title":"Thromb. J."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1350","DOI":"10.1016\/j.fct.2010.02.036","article-title":"Hair growth-promoting effect of Zizyphus jujuba essential oil","volume":"48","author":"Yoon","year":"2010","journal-title":"Food Chem. Toxicol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1472-6882-13-9","article-title":"Hair growth-promoting activity of hot water extract of Thuja Orient","volume":"13","author":"Zhang","year":"2013","journal-title":"BMC Complement Altern. Med."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1739","DOI":"10.1021\/jm0306430","article-title":"Glide: A new approach for rapid, accurate docking and scoring. 1. Method and assessment of docking accuracy","volume":"47","author":"Friesner","year":"2004","journal-title":"J. Med. Chem."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"6177","DOI":"10.1021\/jm051256o","article-title":"Extra precision glide: Docking and scoring incorporating a model of hydrophobic enclosure for protein-ligand complexes","volume":"49","author":"Friesner","year":"2006","journal-title":"J. Med. Chem."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1923","DOI":"10.2174\/1568026614666140929124445","article-title":"Structure-based virtual screening for drug discovery: Principles, applications and recent advances","volume":"14","author":"Lionta","year":"2014","journal-title":"Curr. Top. Med. Chem."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"22129","DOI":"10.1039\/C6CP03670H","article-title":"Assessing the performance of the MM\/PBSA and MM\/GBSA methods. 6. Capability to predict protein\u2013protein binding free energies and re-rank binding poses generated by protein\u2013protein docking","volume":"18","author":"Chen","year":"2016","journal-title":"Phys. Chem. Chem. Phys."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1002\/jcc.21306","article-title":"Drug efficiency indices for improvement of molecular docking scoring functions","volume":"31","author":"Maran","year":"2010","journal-title":"J. Comput. Chem."}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/10\/6\/1081\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T06:09:19Z","timestamp":1760162959000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/10\/6\/1081"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,5,27]]},"references-count":62,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2021,6]]}},"alternative-id":["plants10061081"],"URL":"https:\/\/doi.org\/10.3390\/plants10061081","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,5,27]]}}}