{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T09:16:16Z","timestamp":1791969376460,"version":"4.3.4"},"reference-count":46,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2021,6,2]],"date-time":"2021-06-02T00:00:00Z","timestamp":1622592000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>The Tamarix dioica (T. dioica) is widely used medicinal plant to cure many chronic ailments. T. dioica is being used to manage diabetes mellitus in traditional medicinal system; however, very little scientific evidence is available on this plant in this context. The current study involves the fractionation of crude methanolic extract of T. dioica using n-hexane, ethyl acetate, chloroform, and n-butanol. The screening for antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay was carried out. The in vitro antidiabetic potential was assessed by measuring \u03b1-glucosidase inhibition. Total phenolic and flavonoid contents were also determined for each fraction. The metabolites were identified using highly sensitive and emerging 1H-NMR technique. The results revealed the ethyl acetate fraction as the most potent with DPPH scavenging activity of 84.44 \u00b1 0.21% and \u03b1-glucosidase inhibition with IC50 value of 122.81 \u00b1 2.05 \u00b5g\/mL. The total phenolic and flavonoid content values of 205.45 \u00b1 1.36 mg gallic acid equivalent per gram dried extract and 156.85 \u00b1 1.33 mg quercetin equivalent per gram dried extract were obtained for ethyl acetate fraction. The bucketing of 1H-NMR spectra identified 22 metabolites including some pharmacologically important like tamarixetin, tamaridone, quercetin, rutin, apigenin, catechin, kaempferol, myricetin and isorhamnetin. Leucine, lysine, glutamic acid, aspartic acid, serine, and tyrosine were the major amino acids identified in ethyl acetate fraction. The molecular docking analysis provided significant information on the binding affinity among secondary metabolites and \u03b1-glucosidase. These metabolites were most probably responsible for the antioxidant activity and \u03b1-glucosidase inhibitory potential of ethyl acetate fraction. The study ascertained the ethnomedicinal use of T. dioica to manage diabetes mellitus and may be a helpful lead towards naturopathic mode for anti-hyperglycemia.<\/jats:p>","DOI":"10.3390\/plants10061128","type":"journal-article","created":{"date-parts":[[2021,6,1]],"date-time":"2021-06-01T23:07:03Z","timestamp":1622588823000},"page":"1128","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["The In Vitro \u03b1-Glucosidase Inhibition Activity of Various Solvent Fractions of Tamarix dioica and 1H-NMR Based Metabolite Identification and Molecular Docking Analysis"],"prefix":"10.3390","volume":"10","author":[{"given":"Aamir","family":"Niaz","sequence":"first","affiliation":[{"name":"Department of Chemistry, GC University Lahore, Lahore 54000, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmad","family":"Adnan","sequence":"additional","affiliation":[{"name":"Department of Chemistry, GC University Lahore, Lahore 54000, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5177-0302","authenticated-orcid":false,"given":"Rashida","family":"Bashir","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Education Lahore, Lahore 54000, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Waseem","family":"Mumtaz","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Gujrat, Gujrat 50700, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4624-9683","authenticated-orcid":false,"given":"Syed Ali","family":"Raza","sequence":"additional","affiliation":[{"name":"Department of Chemistry, GC University Lahore, Lahore 54000, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6224-413X","authenticated-orcid":false,"given":"Umer","family":"Rashid","sequence":"additional","affiliation":[{"name":"Institute of Advanced Technology, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4177-4072","authenticated-orcid":false,"given":"Chin Ping","family":"Tan","sequence":"additional","affiliation":[{"name":"Department of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tai Boon","family":"Tan","sequence":"additional","affiliation":[{"name":"Department of Food Services and Management, Faculty of Food Science and Technology, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,6,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"112245","DOI":"10.1016\/j.jep.2019.112245","article-title":"The genus Tamarix: Traditional uses, phytochemistry, and pharmacology","volume":"246","author":"Bahramsoltani","year":"2020","journal-title":"J. Ethnopharmacol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"S2660","DOI":"10.1080\/10942912.2017.1387138","article-title":"Chemical composition of the essential oils from Tamarix dioica and determination of its antibacterial activity","volume":"20","author":"Bughio","year":"2017","journal-title":"Int. J. Food Prop."},{"key":"ref_3","first-page":"40","article-title":"Antifungal activity of Tamarix dioica\u2014An in vitro study","volume":"2","author":"Khan","year":"2004","journal-title":"Gomal J. Med. Sci."},{"key":"ref_4","first-page":"181","article-title":"Phytochemical screening of Tamarix dioica Roxb. ex Roch","volume":"7","author":"Samejo","year":"2013","journal-title":"J. Pharm. Res."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Imtiaz, S.M., Aleem, A., Saqib, F., Ormenisan, A.N., Elena Neculau, A., and Anastasiu, C.V. (2019). The potential involvement of an ATP-dependent potassium channel-opening mechanism in the smooth muscle relaxant properties of Tamarix dioica Roxb. Biomolecules, 9.","DOI":"10.3390\/biom9110722"},{"key":"ref_6","first-page":"81","article-title":"Medicinal plants with antidiabetic potential-A review","volume":"13","author":"ReMukesh","year":"2017","journal-title":"Am-Eurasian. J. Agric. Environ. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1038\/s41440-017-0008-y","article-title":"Hypertension with diabetes mellitus complications","volume":"41","author":"Yamazaki","year":"2018","journal-title":"Hypertens. Res."},{"key":"ref_8","first-page":"224","article-title":"Diabetes mellitus: A review","volume":"3","author":"Deshmukh","year":"2015","journal-title":"Int. J. Pure App. Biosci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1661","DOI":"10.2337\/diab.45.12.1661","article-title":"Thiazolidinediones in the treatment of insulin resistance and type II diabetes","volume":"45","author":"Saltiel","year":"1996","journal-title":"Diabetes"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"313","DOI":"10.2174\/157339911797415585","article-title":"The role of oxidative stress in the pathogenesis of type 2 diabetes mellitus micro-and macrovascular complications: Avenues for a mechanistic-based therapeutic approach","volume":"7","author":"Folli","year":"2011","journal-title":"Curr. Diabetes Rev."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1425","DOI":"10.1007\/s12013-014-0365-y","article-title":"The role of oxidative stress in diabetic neuropathy: Generation of free radical species in the glycation reaction and gene polymorphisms encoding antioxidant enzymes to genetic susceptibility to diabetic neuropathy in population of type I diabetic patients","volume":"71","author":"Babizhayev","year":"2015","journal-title":"Cell Biochem. Biophys."},{"key":"ref_12","first-page":"CD005061","article-title":"Alpha-glucosidase inhibitors for people with impaired glucose tolerance or impaired fasting blood glucose","volume":"18","author":"Lucassen","year":"2006","journal-title":"Cochrane Database Syst. Rev."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"e7022","DOI":"10.7717\/peerj.7022","article-title":"Antioxidant activity, \u03b1-glucosidase inhibition and phytochemical profiling of Hyophorbelagenicaulis leaf extracts","volume":"7","author":"William","year":"2019","journal-title":"PeerJ"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1530","DOI":"10.1002\/jsfa.10771","article-title":"Polyphenol fingerprinting and hypoglycemic attributes of optimized Cycas circinalis leaf extracts","volume":"101","author":"Arshad","year":"2021","journal-title":"J. Sci. Food Agric."},{"key":"ref_15","first-page":"637","article-title":"Antihyperglycemic effect of Conocarpus erectus leaf extract in alloxan-induced diabetic mice","volume":"31","author":"Raza","year":"2018","journal-title":"Pak. J. Pharm. Sci."},{"key":"ref_16","first-page":"2737","article-title":"Antioxidant activity, Hypoglycemic potential and metabolite profiling of Hyophorbe indica leaf extract","volume":"31","author":"William","year":"2018","journal-title":"Pak. J. Pharm. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41598-020-60076-5","article-title":"UHPLC-QTOF-MS\/MS based phytochemical characterization and anti-hyperglycemic prospective of hydro-ethanolic leaf extract of Butea monosperma","volume":"10","author":"Farooq","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2616","DOI":"10.1080\/10942912.2016.1247098","article-title":"Anti-obesity and antioxidant activities of selected medicinal plants and phytochemical profiling of bioactive compounds","volume":"20","author":"Saari","year":"2017","journal-title":"Int. J. Food Prop."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1128","DOI":"10.1016\/j.sjbs.2018.04.007","article-title":"1H NMR based metabolite profiling for optimizing the ethanol extraction of Wolfiporiacocos","volume":"25","author":"Oh","year":"2018","journal-title":"Saudi J. Biol. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Aabideen, Z.U., Mumtaz, M.W., Akhtar, M.T., Mukhtar, H., Raza, S.A., Touqeer, T., and Saari, N. (2020). Anti-obesity attributes; UHPLC-QTOF-MS\/MS-based metabolite profiling and molecular docking insights of Taraxacum officinale. Molecules, 25.","DOI":"10.3390\/molecules25214935"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1186\/s43088-019-0028-6","article-title":"Molecular docking studies of phytochemicals against trehalose\u20136\u2013phosphate phosphatases of pathogenic microbes","volume":"9","author":"Umesh","year":"2020","journal-title":"Beni-Suef Univ. J. Basic Appl. Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"112445","DOI":"10.1016\/j.indcrop.2020.112445","article-title":"Antidiabetic functionality of Vitex negundo L. leaves based on UHPLC-QTOF-MS\/MS based bioactives profiling and molecular docking insights","volume":"152","author":"Nadeem","year":"2020","journal-title":"Ind. Crops Prod."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1","DOI":"10.29252\/qums.13.5.1","article-title":"Effect of hydroalcoholic extract of Tamarix dioica on liver histopathological changes in rats fed with high fat diet","volume":"13","author":"Hajinezhad","year":"2019","journal-title":"Qom Univ. Med. Sci. J."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2568","DOI":"10.1007\/s13197-012-0754-4","article-title":"Influence of different solvents in extraction of phenolic compounds from vegetable residues and their evaluation as natural sources of antioxidants","volume":"51","author":"Babbar","year":"2014","journal-title":"J. Food Sci. Technol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"270","DOI":"10.4161\/oxim.2.5.9498","article-title":"Plant polyphenols as dietary antioxidants in human health and disease","volume":"2","author":"Pandey","year":"2009","journal-title":"Oxidative Med. Cell. Longev."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Tungmunnithum, D., Thongboonyou, A., Pholboon, A., and Yangsabai, A. (2018). Flavonoids and other phenolic compounds from medicinal plants for pharmaceutical and medical aspects: An overview. Medicines, 5.","DOI":"10.3390\/medicines5030093"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1016\/j.talanta.2006.03.050","article-title":"Radical scavenging ability of polyphenolic compounds towards DPPH free radical","volume":"71","author":"Troncoso","year":"2007","journal-title":"Talanta"},{"key":"ref_28","first-page":"403","article-title":"Comparison of antioxidant activity of some medicinally important plants from Pakistan","volume":"12","author":"Raza","year":"2013","journal-title":"Acta Sci. Pol. Technol. Aliment."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"353","DOI":"10.4103\/2221-1691.284430","article-title":"Metabolite profiling and antidiabetic attributes of ultrasonicated leaf extracts of Conocarpu slancifolius","volume":"10","author":"Raza","year":"2020","journal-title":"Asian Pac. J. Trop. Biomed."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"281078","DOI":"10.1155\/2012\/281078","article-title":"Screening of \u03b1-glucosidase inhibitory activity from some plants of Apocynaceae, Clusiaceae, Euphorbiaceae, and Rubiaceae","volume":"2012","author":"Elya","year":"2012","journal-title":"J. Biomed. Biotechnol."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Goboza, M., Meyer, M., Aboua, Y.G., and Oguntibeju, O.O. (2020). In vitro antidiabetic and antioxidant effects of different extracts of Catharanthus roseus and its indole alkaloid, vindoline. Molecules, 25.","DOI":"10.3390\/molecules25235546"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1747","DOI":"10.1002\/cbdv.201600157","article-title":"Evaluation of antioxidant, anticholinesterase, and antidiabetic potential of dry leaves and stems in Tamarix aphylla growing wild in Tunisia","volume":"13","author":"Mahfoudhi","year":"2016","journal-title":"Chem. Biodivers."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2293","DOI":"10.1271\/bbb.110407","article-title":"Effect of environmental conditions on the \u03b1-glucosidase inhibitory activity of mulberry leaves","volume":"75","author":"Nakanishi","year":"2011","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"482","DOI":"10.26656\/fr.2017.4(2).300","article-title":"Antioxidant, iron chelating, lipase inhibition activities and metabolite\u2019s prediction of hyrdoethanolic leaf extract of Conocarpus erectus","volume":"4","author":"Raza","year":"2019","journal-title":"Food Res."},{"key":"ref_35","first-page":"C1080","article-title":"Potent antidiabetic activity and metabolite profiling of Melicopelunu-ankenda leaves","volume":"81","author":"Hamid","year":"2016","journal-title":"J. Food Sci."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"602","DOI":"10.1016\/j.indcrop.2015.09.036","article-title":"Phytochemical and biological features of Phyllanthus niruri and Phyllanthus urinaria harvested at different growth stages revealed by 1H NMR-based metabolomics","volume":"77","author":"Mediani","year":"2015","journal-title":"Ind. Crop Prod."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/S0378-8741(01)00354-3","article-title":"Antioxidant and antimicrobial activities of Tamarix ramosissima","volume":"78","author":"Sultanova","year":"2001","journal-title":"J. Ethnopharmacol."},{"key":"ref_38","first-page":"871","article-title":"Metabolite profiling of Cycas revoluta leaf extract and docking studies on alpha-glucosidase inhibitory molecular targets by phytochemicals","volume":"32","author":"Arshad","year":"2019","journal-title":"Pak. J. Pharm. Sci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"3206","DOI":"10.1007\/s11694-019-00243-z","article-title":"Calotropis procera: UHPLC-QTOF-MS\/MS based profiling of bioactives, antioxidant and anti-diabetic potential of leaf extracts and an insight into molecular docking","volume":"13","author":"Nadeem","year":"2019","journal-title":"J. Food Meas. Charact."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.1016\/j.nut.2011.01.008","article-title":"Effects of flavonoids on \u03b1-glucosidase activity: Potential targets for glucose homeostasis","volume":"27","author":"Pereira","year":"2011","journal-title":"Nutrition"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Rahman, N., Muhammad, I., Khan, H., Aschner, M., Filosa, R., and Daglia, M. (2019). Molecular docking of isolated alkaloids for possible \u03b1-glucosidase inhibition. Biomolecules, 9.","DOI":"10.3390\/biom9100544"},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Ivanovi\u0107, M., Makoter, K., and Islam\u010devi\u0107Razbor\u0161ek, M. (2021). Comparative study of chemical composition and antioxidant activity of essential oils and crude extracts of four characteristic Zingiberaceae Herbs. Plants, 10.","DOI":"10.3390\/plants10030501"},{"key":"ref_43","first-page":"1427","article-title":"Effect of ethanol concentration, extraction time and extraction temperature on the recovery of phenolic compounds and antioxidant capacity of Orthosiphon stamineus extracts","volume":"18","author":"Chew","year":"2011","journal-title":"Int. Food Res. J."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1002\/jsfa.2740610205","article-title":"Gravimetric determination of tannins and their correlations with chemical and protein precipitation methods","volume":"61","author":"Makkar","year":"1993","journal-title":"J. Sci. Food Agric."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"8780704","DOI":"10.1155\/2020\/8780704","article-title":"Total phenolic, flavonoid contents, and antioxidant activities of fruit, seed, and bark extracts of Zanthoxylum armatum DC","volume":"2020","author":"Phuyal","year":"2020","journal-title":"Sci. World J."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1016\/j.foodres.2012.09.022","article-title":"1H-NMR-based metabolomics approach to understanding the drying effects on the phytochemicals in Cosmos caudatus","volume":"49","author":"Mediani","year":"2012","journal-title":"Food Res. Int."}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/10\/6\/1128\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T06:10:01Z","timestamp":1760163001000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/10\/6\/1128"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6,2]]},"references-count":46,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2021,6]]}},"alternative-id":["plants10061128"],"URL":"https:\/\/doi.org\/10.3390\/plants10061128","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,6,2]]}}}