{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,18]],"date-time":"2026-04-18T07:33:10Z","timestamp":1776497590996,"version":"3.51.2"},"reference-count":25,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2021,6,29]],"date-time":"2021-06-29T00:00:00Z","timestamp":1624924800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012190","name":"Ministry of Science and Higher Education of the Russian Federation","doi-asserted-by":"publisher","award":["075-15-2021-310; MD-135.2021.1.4"],"award-info":[{"award-number":["075-15-2021-310; MD-135.2021.1.4"]}],"id":[{"id":"10.13039\/501100012190","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>The medicinal plant Dactylorhiza maculata (L.) So\u00f3 (family Orchidaceae) is used to treat gastritis, colic, gastrointestinal tract, and bladder diseases. This study aimed to investigate the properties and characteristics of the in vitro Dactylorhiza maculata extract. The recommended parameters for producing Dactylorhiza maculata extract were determined: temperature 60 \u00b0C, process duration 60 min, hydro module 1:10. It is recommended to carry out the extraction using an aqueous ethyl alcohol solution with a mass fraction of the parent substance of 70%. It was found that such biologically active substances as\u00a0rutin, quercetin, 3,3\u2032,4\u2032,5,5\u2032,7-hexahydroxyflavonone, 3,3\u2032,4\u2032,5,5\u2032,7-hexahydroxyflavonone-3-O-glycoside, gallic acid, and ferulic acid were dominant in Dactylorhiza maculata.\u00a0A high phosphorus content was noted (2410.8 mg\/kg dry matter).\u00a0The studied Dactylorhizamaculata samples contained a large number of organic acids and water-soluble vitamins. The tested extracts were safe in terms of the content of heavy metals, pesticides, aflatoxin B1, and radionuclides, as well as pathogenic and opportunistic microorganisms; the content was significantly lower than the threshold limit values. The studied complex of biologically active substances from Dactylorhiza maculata extract samples had antimicrobial properties. It was found that the antioxidant activity of the samples was 217.89 \u00b1 10.89 mg AA\/g (AA\u2014ascorbic acid). The high content of bioactive substances and the antimicrobial and antioxidant properties of Dactylorhizamaculata extract samples determine the application potential of this plant as a substitute for growth stimulants and feed antibiotics in the production of feed additives, aiming to increase the physiological and immune status of livestock and poultry.<\/jats:p>","DOI":"10.3390\/plants10071330","type":"journal-article","created":{"date-parts":[[2021,6,29]],"date-time":"2021-06-29T10:52:46Z","timestamp":1624963966000},"page":"1330","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Study of the Properties of In Vitro Dactylorhiza maculata (L.) So\u00f3 (Family Orchidaceae) Extracts"],"prefix":"10.3390","volume":"10","author":[{"given":"Stanislav","family":"Sukhikh","sequence":"first","affiliation":[{"name":"Institute of Living Systems, Immanuel Kant Baltic Federal University, A. Nevskogo Street 14, 236016 Kaliningrad, Russia"},{"name":"Department of Bionanotechnology, Kemerovo State University, Krasnaya Street 6, 650043 Kemerovo, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Svetlana","family":"Noskova","sequence":"additional","affiliation":[{"name":"Institute of Living Systems, Immanuel Kant Baltic Federal University, A. Nevskogo Street 14, 236016 Kaliningrad, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1252-9572","authenticated-orcid":false,"given":"Svetlana","family":"Ivanova","sequence":"additional","affiliation":[{"name":"Natural Nutraceutical Biotesting Laboratory, Kemerovo State University, Krasnaya Street 6, 650043 Kemerovo, Russia"},{"name":"Department of General Mathematics and Informatics, Kemerovo State University, Krasnaya Street 6, 650043 Kemerovo, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6280-1194","authenticated-orcid":false,"given":"Liubov","family":"Skrypnik","sequence":"additional","affiliation":[{"name":"Institute of Living Systems, Immanuel Kant Baltic Federal University, A. Nevskogo Street 14, 236016 Kaliningrad, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8374-3907","authenticated-orcid":false,"given":"Artem","family":"Pungin","sequence":"additional","affiliation":[{"name":"Institute of Living Systems, Immanuel Kant Baltic Federal University, A. Nevskogo Street 14, 236016 Kaliningrad, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"Ulrikh","sequence":"additional","affiliation":[{"name":"Kuzbass State Agricultural Academy, Markovtseva Street 5, 650056 Kemerovo, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5284-4748","authenticated-orcid":false,"given":"Evgeny","family":"Chupakhin","sequence":"additional","affiliation":[{"name":"Institute of Living Systems, Immanuel Kant Baltic Federal University, A. Nevskogo Street 14, 236016 Kaliningrad, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4921-8997","authenticated-orcid":false,"given":"Olga","family":"Babich","sequence":"additional","affiliation":[{"name":"Institute of Living Systems, Immanuel Kant Baltic Federal University, A. Nevskogo Street 14, 236016 Kaliningrad, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,6,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"98","DOI":"10.31254\/phyto.2017.6206","article-title":"Antiviral activities of Cucumis metuliferus fruits alkaloids on infectious bursal disease virus (IBDV)","volume":"6","author":"Anyanwu","year":"2017","journal-title":"J. Phytopharmacol."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Babich, O., Sukhikh, S., Prosekov, A., Asyakina, L., and Ivanova, S. (2020). Medicinal Plants to Strengthen Immunity during a Pandemic. Pharmaceuticals, 13.","DOI":"10.3390\/ph13100313"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Babich, O., Sukhikh, S., Pungin, A., Ivanova, S., Asyakina, L., and Prosekov, A. (2020). Modern Trends in the In Vitro Production and Use of Callus, Suspension Cells and Root Cultures of Medicinal Plants. Molecules, 25.","DOI":"10.3390\/molecules25245805"},{"key":"ref_4","first-page":"44","article-title":"Molecular and morphological approaches to studying the Dactylorhiza genus in the Komi Republic","volume":"43","author":"Kirillova","year":"2018","journal-title":"Vestn. Tomsk. Gos. Univ. Biol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.sajb.2018.09.011","article-title":"Acetylcholinesterase inhibitors from southern African plants: An overview of ethnobotanical, pharmacological potential and phytochemical research including and beyond Alzheimer\u2019s disease treatment","volume":"120","author":"Masondo","year":"2019","journal-title":"S. Afr. J. Bot."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"112803","DOI":"10.1016\/j.jep.2020.112803","article-title":"Secondary metabolites (essential oils) from sand-dune plants induce cytotoxic effects in cancer cells","volume":"258","author":"Beeby","year":"2020","journal-title":"J. Ethnopharmacol."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Alonso, A., Dominguez, F., Ruiz, A.J., Campos, N., Zapata, J.R., and Carranza, C. (2017). Medicinal plants from north and Central America and the Caribbean considered toxic for humans: The other side of the coin. Med. Evid. Based Complement. Altern. Med., 2017.","DOI":"10.1155\/2017\/9439868"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Guti\u00e9rrez-Grijalva, E.P., Picos-Salas, M.A., Leyva-L\u00f3pez, N., Criollo-Mendoza, M.S., Vazquez-Olivo, G., and Heredia, J.B. (2018). Flavonoids and phenolic acids from oregano: Occurrence, biological activity and health benefits. Plants, 7.","DOI":"10.3390\/plants7010002"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"209","DOI":"10.11648\/j.cmr.20170606.16","article-title":"Phytochemical and Proximate Analysis of Some Medicinal Leaves","volume":"6","author":"Aborisade","year":"2017","journal-title":"CMR"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"33","DOI":"10.2298\/ZMSPN1222033R","article-title":"Morphological variabillity of populations of the Dactylorhiza maculata (L.) So\u00f3 1962 and D. majalis (Reichenb.) P. F. Hunt et Summerhayes 1965 (Orchidales, Orchidaceae) with Stara planina. Matica Srpska","volume":"122","author":"Radak","year":"2012","journal-title":"Zbornik Matice Srpske za Prirodne Nauke"},{"key":"ref_11","first-page":"592","article-title":"Orchids: A review of uses in traditional medicine, its phytochemistry and pharmacology","volume":"4","author":"Gutierrez","year":"2010","journal-title":"J. Med. Plants Res."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1134\/S1062359019030063","article-title":"Study of Dactylorhiza Seeds (D. baltica and D. maculata) from the Orchid Collection of the Cryobank at Timiryazev institute of plant physiology Russian Academy of Sciences","volume":"46","author":"Nikishina","year":"2019","journal-title":"Biol. Bull."},{"key":"ref_13","first-page":"135","article-title":"Amino acid composition of vegetative organs Dactylorhiza maculata (L.) So\u00f3 (Orchidaceae)","volume":"2","author":"Sechin","year":"2019","journal-title":"Khimiya Rastit. Syr\u2019ya"},{"key":"ref_14","first-page":"6","article-title":"Effect of supernatant of associative bacteria of the genus Pseudomonas on germination, morphogenesis and growth of Dactylorhiza incarnata (L.) So\u00f3 (Orchidaceae) in vitro","volume":"51","author":"Sidorov","year":"2020","journal-title":"Vestn. Tomsk. Gos. Univ. Biol."},{"key":"ref_15","unstructured":"Fanali, S., Haddad, P.R., Poole, C.F., and Riekkola, M.-L. (2017). Modeling of preparative liquid chromatography. Liquid Chromatography, Elsevier. [2nd ed.]."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1143","DOI":"10.3389\/fpls.2015.01143","article-title":"Heavy metal tolerance in plants: Role of transcriptomics, proteomics, metabolomics, and ionomics","volume":"6","author":"Singh","year":"2016","journal-title":"Front. Plant. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"El-Shemy, H. (2017). From medicinal plant raw material to herbal remedies. Aromatic and Medicinal Plants, IntechOpun.","DOI":"10.5772\/67161"},{"key":"ref_18","unstructured":"Spiridovich, E.V., Paromchik, I.I., and Fomenko, T.I. (2013). Soderzhanie efirnogo masla Monarda fistulosa L., inducirovannogo v usloviayh centralnogo botanichskogo sada NAN Belarusi [The content of Monarda fistulosa L. essential oil, introduced in the conditions of the Central Botanical Garden of the National Academy of Sciences of Belarus]. Biologicheski Aktivnye Veschestva Rasteniy\u2014Izuchenie i Ispol\u2019zovanie [Biologically Active Substances of Plants-Study and Using], GNU \u201cCentral Botanical Garden of the Academy of Sciences of Belarus\u201d. (In Russian)."},{"key":"ref_19","first-page":"439","article-title":"Carotenoids, tocopherols, organic acids, charbohydrate and mineral content in different medicinal plant extracts","volume":"73","author":"Mihaylova","year":"2018","journal-title":"Z. Nat. C. J. Biosci."},{"key":"ref_20","first-page":"837","article-title":"Histo-anatomical researches, polyphenolic profile and biological activities of the aerial parts and tubers of Dactylorhiza maculata L. SO\u00d3. (Orchidaceae)","volume":"65","author":"Ielciu","year":"2017","journal-title":"Farmacia"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Salmer\u00f3n-Manzano, E., and Manzano-Agugliaro, F. (2020). Worldwide Research on Low Cost Technologies through Bibliometric Analysis. Inventions, 5.","DOI":"10.3390\/inventions5010009"},{"key":"ref_22","unstructured":"Alhajj, M., and Farhana, A. (2021, June 17). Enzyme Linked Immunosorbent Assay. StatPearls, Available online: https:\/\/www.ncbi.nlm.nih.gov\/books\/NBK555922\/."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1515\/intox-2016-0012","article-title":"Organochlorine pesticides, their toxic effects on living organisms and their fate in the environment","volume":"9","author":"Jayaraj","year":"2016","journal-title":"Interdiscip. Toxicol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.sajb.2018.07.024","article-title":"The role of the South African Journal of Botany as a vehicle to promote medicinal plant research\u2013A bibliometric appraisal","volume":"122","author":"Viljoen","year":"2019","journal-title":"S. Afr. J. Bot."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Salehi, B., Sharopov, F., Martorell, M., Rajkovic, J., Ademiluyi, A.O., and Sharifi, M. (2018). Phytochemicals in Helicobacter pylori infections: What are we doing now?. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19082361"}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/10\/7\/1330\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T06:27:06Z","timestamp":1760164026000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/10\/7\/1330"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6,29]]},"references-count":25,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2021,7]]}},"alternative-id":["plants10071330"],"URL":"https:\/\/doi.org\/10.3390\/plants10071330","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,6,29]]}}}