{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T12:16:51Z","timestamp":1784204211250,"version":"3.55.0"},"reference-count":55,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2021,7,7]],"date-time":"2021-07-07T00:00:00Z","timestamp":1625616000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004685","name":"Universidad Aut\u00f3noma de Nuevo Le\u00f3n","doi-asserted-by":"publisher","award":["04-093765-FAR-11\/250-FCQ-UANL"],"award-info":[{"award-number":["04-093765-FAR-11\/250-FCQ-UANL"]}],"id":[{"id":"10.13039\/501100004685","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007476","name":"Universidad Aut\u00f3noma del Estado de Morelos","doi-asserted-by":"publisher","award":["LANEM Project"],"award-info":[{"award-number":["LANEM Project"]}],"id":[{"id":"10.13039\/501100007476","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Cissus\u00a0incisa leaves have been traditionally used in Mexican traditional medicine to treat certain cancerous illness. This study explored the metabolomic profile of this species using untargeted technique. Likewise, it determined the cytotoxic activity and interpreted all data by computational tools. The metabolomic profile was developed through UHPLC-QTOF-MS\/MS for dereplication purposes. MetaboAnalyst database was used in metabolic pathway analysis and the network topological analysis. Hexane, chloroform\/methanol, and aqueous extracts were evaluated on HepG2, Hep3B, HeLa, PC3, A549, and MCF7 cancer cell lines and IHH immortalized hepatic cells, using Cell Titer proliferation assay kit. Hexane extract was the most active against Hep3B (IC50 = 27 \u00b1 3 \u03bcg\/mL), while CHCl3\/MeOH extract was the most selective (SI = 2.77) on the same cell line. A Principal Component Analysis (PCA) showed similar profiles between the extracts, while a Venn diagram revealed 80 coincident metabolites between the bioactive extracts. The sesquiterpenoid and triterpenoid biosynthesis pathway was the most significant identified. The Network Pharmacology (NP) approach revealed several targets for presqualene diphosphate, phytol, stearic acid, \u03b4-tocopherol, ursolic acid and \u03b3-linolenic acid, involved in cellular processes such as apoptosis. This work highlights the integration of untargeted metabolomic profile and cytotoxic activity to explore plant extracts, and the NP approach to interpreting the experimental results.<\/jats:p>","DOI":"10.3390\/plants10071389","type":"journal-article","created":{"date-parts":[[2021,7,6]],"date-time":"2021-07-06T23:53:51Z","timestamp":1625615631000},"page":"1389","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Metabolomic Profile and Cytotoxic Activity of Cissus\u00a0incisa Leaves Extracts"],"prefix":"10.3390","volume":"10","author":[{"given":"Deyani","family":"Nocedo-Mena","sequence":"first","affiliation":[{"name":"Facultad de Ciencias Qu\u00edmicas, Universidad Aut\u00f3noma de Nuevo Le\u00f3n, Av. Universidad S\/N, Ciudad Universitaria, San Nicol\u00e1s de los Garza 66451, Nuevo Le\u00f3n, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8875-8734","authenticated-orcid":false,"given":"Mar\u00eda Yolanda","family":"R\u00edos","sequence":"additional","affiliation":[{"name":"Centro de Investigaciones Qu\u00edmicas-IICBA, Universidad Aut\u00f3noma del Estado de Morelos, Av. Universidad 1001, Cuernavaca 62209, Morelos, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4696-7359","authenticated-orcid":false,"given":"M. \u00c1ngeles","family":"Ram\u00edrez-Cisneros","sequence":"additional","affiliation":[{"name":"Centro de Investigaciones Qu\u00edmicas-IICBA, Universidad Aut\u00f3noma del Estado de Morelos, Av. Universidad 1001, Cuernavaca 62209, Morelos, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4456-1235","authenticated-orcid":false,"given":"Leticia","family":"Gonz\u00e1lez-Maya","sequence":"additional","affiliation":[{"name":"Facultad de Farmacia, Universidad Aut\u00f3noma del Estado de Morelos, Av. Universidad 1001, Cuernavaca 62209, Morelos, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica N.","family":"S\u00e1nchez-Carranza","sequence":"additional","affiliation":[{"name":"Facultad de Farmacia, Universidad Aut\u00f3noma del Estado de Morelos, Av. Universidad 1001, Cuernavaca 62209, Morelos, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8179-7231","authenticated-orcid":false,"given":"Mar\u00eda del Rayo","family":"Camacho-Corona","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Qu\u00edmicas, Universidad Aut\u00f3noma de Nuevo Le\u00f3n, Av. Universidad S\/N, Ciudad Universitaria, San Nicol\u00e1s de los Garza 66451, Nuevo Le\u00f3n, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,7,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Kim, C., and Kim, B. (2018). Anti-cancer natural products and their bioactive compounds inducing ER stress-mediated apoptosis: A review. 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