{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,21]],"date-time":"2026-06-21T07:03:15Z","timestamp":1782025395868,"version":"3.54.5"},"reference-count":71,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2021,8,11]],"date-time":"2021-08-11T00:00:00Z","timestamp":1628640000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Kuok Foundation Berhad","award":["4393\/000"],"award-info":[{"award-number":["4393\/000"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>The lack of specific treatment for chikungunya fever makes the need for anti-chikungunya virus agents more crucial. This study was conducted to evaluate 132 extracts obtained by sequential solvent extraction from 21 medicinal plants for cytopathic effect inhibitory activity using virus-infected Vero cells in two different sample introduction modes. Among the extracts, 42 extracts (31.8%) from 12 plants in the concurrent mode and three extracts (2.3%) from a plant in the non-concurrent mode displayed strong cytopathic effect inhibitory activity (cell viability \u226570%). Viral load quantification analysis unveiled that the extracts of Clinacanthus nutans (chloroform, ethyl acetate, and ethanol), Hydrocotyle sibthorpioides (ethanol), and Ocimum americanum (ethanol and methanol) hindered the release of viral progeny from the infected cells while the extracts of Ficus deltoidea (ethanol), Gynura bicolor (water), H. sibthorpioides (water), and O. americanum (chloroform and ethyl acetate) blocked the entry of virus into the cells. The extracts of Diodella sarmentosa (ethyl acetate), Diplazium esculentum (chloroform, ethyl acetate, and ethanol), and G. bicolor (ethanol) possessed virucidal effect and caused 5.41-log to 6.63-log reductions of viral load compared to the virus control. The results indicate that these medicinal plants are potential sources of anti-chikungunya virus agents that have varied modes of action.<\/jats:p>","DOI":"10.3390\/plants10081658","type":"journal-article","created":{"date-parts":[[2021,8,11]],"date-time":"2021-08-11T21:48:12Z","timestamp":1628718492000},"page":"1658","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Mode-Dependent Antiviral Activity of Medicinal Plant Extracts against the Mosquito-Borne Chikungunya Virus"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7820-595X","authenticated-orcid":false,"given":"Sze Mun","family":"Chan","sequence":"first","affiliation":[{"name":"Department of Allied Health Sciences, Faculty of Science, Universiti Tunku Abdul Rahman, Bandar Barat, Kampar 31900, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kong Soo","family":"Khoo","sequence":"additional","affiliation":[{"name":"Department of Chemical Science, Faculty of Science, Universiti Tunku Abdul Rahman, Bandar Barat, Kampar 31900, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0478-0425","authenticated-orcid":false,"given":"Shamala Devi","family":"Sekaran","sequence":"additional","affiliation":[{"name":"Faculty of Medicine & Health Sciences, UCSI University, Bandar Springhill, Port Dickson 71010, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5949-491X","authenticated-orcid":false,"given":"Nam Weng","family":"Sit","sequence":"additional","affiliation":[{"name":"Department of Allied Health Sciences, Faculty of Science, Universiti Tunku Abdul Rahman, Bandar Barat, Kampar 31900, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,8,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Richman, D.D., Whitley, R.J., and Hayden, F.G. (2017). Alphaviruses. Clinical Virology, ASM Press. [4th ed.].","DOI":"10.1128\/9781555819439"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1017\/S0022172400044442","article-title":"The Newala epidemic: III. The virus: Isolation, pathogenic properties and relationship to the epidemic","volume":"54","author":"Ross","year":"1956","journal-title":"J. Hyg."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1003","DOI":"10.1016\/j.idc.2019.08.006","article-title":"Chikungunya: Epidemiology, pathogenesis, clinical features, management, and prevention","volume":"33","author":"Vairo","year":"2019","journal-title":"Infect. Dis. Clin. N. Am."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1172\/JCI84417","article-title":"Chikungunya virus: Epidemiology, replication, disease mechanisms, and prospective intervention strategies","volume":"127","author":"Silva","year":"2017","journal-title":"J. Clin. Investig."},{"key":"ref_5","unstructured":"Centers for Disease Control and Prevention (2021, January 03). Chikungunya Virus, Available online: https:\/\/www.cdc.gov\/chikungunya\/index.html."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Puntasecca, C.J., King, C.H., and LaBeaud, A.D. (2021). Measuring the global burden of chikungunya and Zika viruses: A systematic review. PLoS Negl. Trop. Dis., 15.","DOI":"10.1371\/journal.pntd.0009055"},{"key":"ref_7","unstructured":"European Centre for Disease Prevention and Control (2021, January 03). Communicable Disease Threats Report, Week 51, 13\u201319 December 2020. Available online: https:\/\/www.ecdc.europa.eu\/en\/publications-data\/communicable-disease-threats-report-13-19-december-2020-week-51."},{"key":"ref_8","unstructured":"Ministry of Health Malaysia (2021, January 03). Kenyataan Akhbar Ketua Pengarah Kesihatan Malaysia Mengenai Situasi Semasa Deman Denggi, Zika Dan Chikungunya Di Malaysia\u2014Minggu Ke 51\/2020, (In Malay)."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Ferreira, F.C., da Silva, A.S., Recht, J., Guaraldo, L., Moreira, M.E., de Siqueira, A.M., Gerardin, P., and Brasil, P. (2021). Vertical transmission of chikungunya virus: A systematic review. PLoS ONE, 16.","DOI":"10.1371\/journal.pone.0249166"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"e00350-19","DOI":"10.1128\/JCM.00350-19","article-title":"Beyond fever and pain: Diagnostic methods for chikungunya virus","volume":"57","author":"Natrajan","year":"2019","journal-title":"J. Clin. Microbiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"345","DOI":"10.3389\/fcimb.2018.00345","article-title":"Cellular and molecular immune response to chikungunya virus infection","volume":"8","author":"Tanabe","year":"2018","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.tmaid.2017.01.004","article-title":"Long-term sequelae of chikungunya virus disease: A systematic review","volume":"15","author":"Nelen","year":"2017","journal-title":"Travel Med. Infect. Dis."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"e00104-16","DOI":"10.1128\/CMR.00104-16","article-title":"Vaccine and therapeutic options to control chikungunya virus","volume":"31","author":"Powers","year":"2017","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2881","DOI":"10.3389\/fmicb.2019.02881","article-title":"Recent progress in vaccine development against chikungunya virus","volume":"10","author":"Gao","year":"2019","journal-title":"Front. Microbiol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"618624","DOI":"10.3389\/fpubh.2020.618624","article-title":"Current and promising antivirals against chikungunya virus","volume":"8","author":"Hucke","year":"2020","journal-title":"Front. Public Health"},{"key":"ref_16","unstructured":"World Health Organization (1993). Guidelines on the Conservation of Medicinal Plants, International Union for the Conservation of Nature."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1016\/j.micpath.2018.08.034","article-title":"Role of secondary metabolites in plant defense against pathogens","volume":"124","author":"Zaynab","year":"2018","journal-title":"Microb. Pathog."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.jep.2017.08.005","article-title":"Anti-HIV activity of southern African plants: Current developments, phytochemistry and future research","volume":"210","author":"Prinsloo","year":"2018","journal-title":"J. Ethnopharmacol."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Salehi, B., Kumar, N.V.A., \u015eener, B., Sharifi-Rad, M., K\u0131l\u0131\u00e7, M., Mahady, G.B., Vlaisavljevic, S., Iriti, M., Kobarfard, F., and Setzer, W.N. (2018). Medicinal plants used in the treatment of human immunodeficiency virus. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19051459"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"811","DOI":"10.1002\/ptr.6024","article-title":"Antiviral potential of medicinal plants against HIV, HSV, influenza, hepatitis, and coxsackievirus: A systematic review","volume":"32","author":"Akram","year":"2018","journal-title":"Phytother. Res."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Mohan, S., Elhassan Taha, M.M., Makeen, H.A., Alhazmi, H.A., Al Bratty, M., Sultana, S., Ahsan, W., Najmi, A., and Khalid, A. (2020). Bioactive natural antivirals: An updated review of the available plants and isolated molecules. Molecules, 25.","DOI":"10.3390\/molecules25214878"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1007\/s13659-018-0178-6","article-title":"The progress of anti-HBV constituents from medicinal plants in China","volume":"8","author":"Geng","year":"2018","journal-title":"Nat. Prod. Bioprospect."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Saleh, M.S.M., and Kamisah, Y. (2020). Potential medicinal plants for the treatment of dengue fever and severe acute respiratory syndrome-coronavirus. Biomolecules, 11.","DOI":"10.3390\/biom11010042"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"33","DOI":"10.21010\/ajtcam.v14i4S.5","article-title":"Anti-viral effects of medicinal plants in the management of dengue: A systematic review","volume":"14","author":"Frederico","year":"2017","journal-title":"Afr. J. Tradit. Complement. Altern. Med."},{"key":"ref_25","first-page":"175","article-title":"Investigation of twenty selected medicinal plants from Malaysia for anti-Chikungunya virus activity","volume":"19","author":"Chan","year":"2016","journal-title":"Int. Microbiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"622","DOI":"10.1016\/j.ijantimicag.2018.07.017","article-title":"Antiplasmodial, anti-chikungunya virus and antioxidant activities of 64 endemic plants from the Mascarene Islands","volume":"52","author":"Ledoux","year":"2018","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.antiviral.2014.11.001","article-title":"The green tea catechin, epigallocatechin gallate inhibits chikungunya virus infection","volume":"113","author":"Weber","year":"2015","journal-title":"Antivir. Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/j.antiviral.2017.03.014","article-title":"Curcumin inhibits Zika and chikungunya virus infection by inhibiting cell binding","volume":"142","author":"Mounce","year":"2017","journal-title":"Antivir. Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1128\/AAC.01467-12","article-title":"Inhibition of chikungunya virus replication by harringtonine, a novel antiviral that suppresses viral protein expression","volume":"57","author":"Kaur","year":"2013","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.jchromb.2017.12.005","article-title":"Influence of direct and sequential extraction methodology on metabolic profiling","volume":"1073","author":"Harnly","year":"2018","journal-title":"J. Chromatogr. B"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1128\/CMR.12.4.564","article-title":"Plant products as antimicrobial agents","volume":"12","author":"Cowan","year":"1999","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/j.phytochem.2016.11.007","article-title":"Ailanthusins A-G and nor-lupane triterpenoids from Ailanthus triphysa","volume":"134","author":"Thongnest","year":"2017","journal-title":"Phytochemistry"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2697","DOI":"10.1002\/jsfa.4516","article-title":"Evaluating the toxic and beneficial effects of jering beans (Archidendron jiringa) in normal and diabetic rats","volume":"91","author":"Shukri","year":"2011","journal-title":"J. Sci. Food Agric."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"114143","DOI":"10.1016\/j.jep.2021.114143","article-title":"Phytochemistry and pharmacological activities of Arundina graminifolia (D.Don) Hochr. and other common Orchidaceae medicinal plants","volume":"276","author":"Zhang","year":"2021","journal-title":"J. Ethnopharmacol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1241","DOI":"10.1002\/ptr.6076","article-title":"A comprehensive review of phytochemical profile, bioactives for pharmaceuticals, and pharmacological attributes of Azadirachta indica","volume":"32","author":"Saleem","year":"2018","journal-title":"Phytother. Res."},{"key":"ref_36","first-page":"153","article-title":"A review of the taxonomy, ethnobotany, phytochemistry and pharmacology of Basella alba (Basellaceae)","volume":"4","author":"Deshmukh","year":"2014","journal-title":"J. Appl. Pharm. Sci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"74","DOI":"10.2174\/1570163815666180308142912","article-title":"Beta vulgaris\u2014A mini review of traditional uses in Iran, phytochemistry and pharmacology","volume":"16","author":"Hamedi","year":"2019","journal-title":"Curr. Drug Discov. Technol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/j.jep.2017.05.007","article-title":"Ethnopharmacological uses, phytochemistry, biological activities, and therapeutic applications of Clinacanthus nutans (Burm. f.) Lindau: A comprehensive review","volume":"206","author":"Kamarudin","year":"2017","journal-title":"J. Ethnopharmacol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"871","DOI":"10.2174\/1381612825666190402163940","article-title":"Ethnobotany, phytochemistry and traditional uses of Curcuma spp. and pharmacological profile of two important species (C. longa and C. zedoaria): A review","volume":"25","author":"Ayati","year":"2019","journal-title":"Curr. Pharm. Des."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/S0378-8741(98)00060-9","article-title":"Evaluation of Nigerian traditional medicines: II. Effects of some Nigerian folk remedies on peptic ulcer","volume":"62","author":"Akah","year":"1998","journal-title":"J. Ethnopharmacol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1016\/j.jep.2012.11.029","article-title":"An ethnobotanical study of medicinal plants and traditional therapies on Batan Island, the Philippines","volume":"145","author":"Abe","year":"2013","journal-title":"J. Ethnopharmacol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"902734","DOI":"10.1155\/2014\/902734","article-title":"Ficus deltoidea Jack: A review on its phytochemical and pharmacological importance","volume":"2014","author":"Bunawan","year":"2014","journal-title":"Evid. Based Complement. Alternat. Med."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"958407","DOI":"10.1155\/2013\/958407","article-title":"Antioxidant capacity, cytotoxicity, and acute oral toxicity of Gynura bicolor","volume":"2013","author":"Teoh","year":"2013","journal-title":"Evid. Based Complement. Altern. Med."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1016\/j.jep.2010.04.036","article-title":"Traditionally used Thai medicinal plants: In vitro anti-inflammatory, anticancer and antioxidant activities","volume":"130","author":"Siriwatanametanon","year":"2010","journal-title":"J. Ethnopharmacol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1847","DOI":"10.1002\/ptr.6924","article-title":"Journey of Hydrocotyle sibthorpioides Lam.: From traditional utilization to modern therapeutics\u2014A review","volume":"35","author":"Hazarika","year":"2021","journal-title":"Phytother. Res."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Lim, T.K. (2013). Edible Medicinal and Non-Medicinal Plants: Fruits, Springer Science + Business Media.","DOI":"10.1007\/978-94-007-5653-3"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"108610","DOI":"10.1016\/j.foodres.2019.108610","article-title":"Comprehensive approaches on the chemical constituents and pharmacological properties of flowers and leaves of American basil (Ocimum americanum L.)","volume":"125","author":"Zengin","year":"2019","journal-title":"Food Res. Int."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Saleh, M.S.M., Jalil, J., Zainalabidin, S., Asmadi, A.Y., Mustafa, N.H., and Kamisah, Y. (2021). Genus Parkia: Phytochemical, medicinal uses, and pharmacological properties. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22020618"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1016\/S0254-6272(14)60018-2","article-title":"Parsley: A review of ethnopharmacology, phytochemistry and biological activities","volume":"33","author":"Farzaei","year":"2013","journal-title":"J. Tradit. Chin. Med."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"645","DOI":"10.4103\/1995-7645.248321","article-title":"Salacca zalacca: A short review of the palm botany, pharmacological uses and phytochemistry","volume":"11","author":"Saleh","year":"2018","journal-title":"Asian Pac. J. Trop. Med."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1016\/j.foodchem.2018.09.146","article-title":"Chayote (Sechium edule): A review of nutritional composition, bioactivities and potential applications","volume":"275","author":"Vieira","year":"2019","journal-title":"Food Chem."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1482","DOI":"10.5530\/pj.2020.12.203","article-title":"Ethnobotany and traditional knowledge of Acanthaceae in Peninsular Malaysia: A review","volume":"12","author":"Zakaria","year":"2020","journal-title":"Pharmacogn. J."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Bernard, E., Solignat, M., Gay, B., Chazal, N., Higgs, S., Devaux, C., and Briant, L. (2010). Endocytosis of chikungunya virus into mammalian cells: Role of clathrin and early endosomal compartments. PLoS ONE, 5.","DOI":"10.1371\/journal.pone.0011479"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"817","DOI":"10.1002\/jmv.21663","article-title":"Assessment of in vitro prophylactic and therapeutic efficacy of chloroquine against Chikungunya virus in Vero cells","volume":"82","author":"Khan","year":"2010","journal-title":"J. Med. Virol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"2183","DOI":"10.1021\/np300637t","article-title":"Prostratin and 12-O-tetradecanoylphorbol 13-acetate are potent and selective inhibitors of Chikungunya virus replication","volume":"75","author":"Bourjot","year":"2012","journal-title":"J. Nat. Prod."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Scholte, F.E., Tas, A., Martina, B.E., Cordioli, P., Narayanan, K., Makino, S., Snijder, E.J., and van Hemert, M.J. (2013). Characterization of synthetic Chikungunya viruses based on the consensus sequence of recent E1-226V isolates. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0071047"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.virol.2019.04.007","article-title":"Evaluation of medicinal herbs for Anti-CHIKV activity","volume":"533","author":"Raghavendhar","year":"2019","journal-title":"Virology"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1857","DOI":"10.1016\/j.bmc.2009.01.059","article-title":"Bioactive constituents from the leaves of Clinacanthus nutans Lindau","volume":"17","author":"Sakdarat","year":"2009","journal-title":"Bioorg. Med. Chem."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1016\/S2221-1691(13)60064-7","article-title":"The identities and anti-herpes simplex virus activity of Clinacanthus nutans and Clinacanthus siamensis","volume":"3","author":"Kunsorn","year":"2013","journal-title":"Asian Pac. J. Trop. Biomed."},{"key":"ref_60","first-page":"240","article-title":"The anti-papillomavirus infectivity of Clinacanthus nutans compounds","volume":"26","author":"Sookmai","year":"2011","journal-title":"Srinagarind Med. J."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"20382","DOI":"10.3390\/molecules191220382","article-title":"Chemical constituents and bioactivities of Clinacanthus nutans aerial parts","volume":"19","author":"Tu","year":"2014","journal-title":"Molecules"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"596109","DOI":"10.1155\/2015\/596109","article-title":"The effect of Hydrocotyle sibthorpioides Lam. extracts on in vitro dengue replication","volume":"2015","author":"Husin","year":"2015","journal-title":"Evid. Based Complement. Altern. Med."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"568","DOI":"10.1016\/j.jep.2013.09.009","article-title":"Isolation and identification of an anti-hepatitis B virus compound from Hydrocotyle sibthorpioides Lam","volume":"150","author":"Huang","year":"2013","journal-title":"J. Ethnopharmacol."},{"key":"ref_64","first-page":"860","article-title":"Anti-herpes simplex virus activity of extracts from the culinary herbs Ocimum sanctum L., Ocimum basilicum L. and Ocimum americanum L","volume":"10","author":"Yucharoen","year":"2011","journal-title":"Afr. J. Biotechnol."},{"key":"ref_65","first-page":"B42","article-title":"In vitro anti-viral activity of Centella asiatica L., Curcuma longa L. and Strobilanthes crispus L. against herpes virus","volume":"5","author":"Hanisa","year":"2014","journal-title":"Int. J. Pharma Bio Sci."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1076\/phbi.39.5.364.5892","article-title":"Multiple antiviral activities of endemic medicinal plants used by Berber peoples of Morocco","volume":"39","author":"Mouhajir","year":"2001","journal-title":"Pharm. Biol."},{"key":"ref_67","first-page":"128","article-title":"Antimicrobial, antiviral and cytotoxic activities of selected marine organisms collected from the coastal areas of Malaysia","volume":"26","author":"Chan","year":"2018","journal-title":"J. Mar. Sci. Technol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1093\/oxfordjournals.aje.a118408","article-title":"A simple method of estimating fifty per cent endpoints","volume":"27","author":"Reed","year":"1938","journal-title":"Am. J. Epidemiol."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"1991","DOI":"10.4314\/tjpr.v14i11.6","article-title":"Interactions between plant extracts and cell viability indicators during cytotoxicity testing: Implications for ethnopharmacological studies","volume":"14","author":"Chan","year":"2015","journal-title":"Trop. J. Pharm. Res."},{"key":"ref_70","first-page":"611","article-title":"Development and evaluation of a one-step SYBR-Green I-based realtime RT-PCR assay for the detection and quantification of Chikungunya virus in human, monkey and mosquito samples","volume":"27","author":"Ali","year":"2010","journal-title":"Trop. Biomed."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"6463","DOI":"10.1093\/nar\/18.21.6463","article-title":"Improved synthesis of full-length RNA probe at reduced incubation temperatures","volume":"18","author":"Krieg","year":"1990","journal-title":"Nucleic Acids Res."}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/10\/8\/1658\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T06:44:24Z","timestamp":1760165064000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/10\/8\/1658"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,8,11]]},"references-count":71,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2021,8]]}},"alternative-id":["plants10081658"],"URL":"https:\/\/doi.org\/10.3390\/plants10081658","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,8,11]]}}}