{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T16:01:14Z","timestamp":1779206474211,"version":"3.51.4"},"reference-count":71,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2021,10,12]],"date-time":"2021-10-12T00:00:00Z","timestamp":1633996800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2017YFD0200100"],"award-info":[{"award-number":["2017YFD0200100"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["2662019PY013"],"award-info":[{"award-number":["2662019PY013"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Nitrogen (N) is an essential macronutrient for plants. However, little is known about the molecular regulation of N assimilation in Brassica napus, one of the most important oil crops worldwide. Here, we carried out a comprehensive genome-wide analysis of the N assimilation related genes (NAGs) in B. napus. A total of 67 NAGs were identified encoding major enzymes involved in N assimilation, including asparagine synthetase (AS), glutamate dehydrogenase (GDH), glutamine oxoglutarate aminotransferase (GOGAT), glutamine synthetase (GS), nitrite reductase (NiR), nitrate reductase (NR). The syntenic analysis revealed that segmental duplication and whole-genome duplication were the main expansion pattern during gene evolution. Each NAG family showed different degrees of differentiation in characterization, gene structure, conserved motifs and cis-elements. Furthermore, diverse responses of NAG to multiple nutrient stresses were observed. Among them, more NAGs were regulated by N deficiency and ammonium toxicity than by phosphorus and potassium deprivations. Moreover, 12 hub genes responding to N starvation were identified, which may play vital roles in N utilization. Taken together, our results provide a basis for further functional research of NAGs in rapeseed N assimilation and also put forward new points in their responses to contrasting nutrient stresses.<\/jats:p>","DOI":"10.3390\/plants10102160","type":"journal-article","created":{"date-parts":[[2021,10,13]],"date-time":"2021-10-13T06:38:41Z","timestamp":1634107121000},"page":"2160","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Nitrogen Assimilation Related Genes in Brassicanapus: Systematic Characterization and Expression Analysis Identified Hub Genes in Multiple Nutrient Stress Responses"],"prefix":"10.3390","volume":"10","author":[{"given":"Xuyou","family":"He","sequence":"first","affiliation":[{"name":"Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture and Rural Affairs\/State Environmental Protection, Huazhong Agricultural University, Wuhan 430070, China"},{"name":"Key Laboratory of Soil Health and Green Remediation, Ministry of Ecology and Environment\/Micro-Element Research Center\/College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture and Rural Affairs\/State Environmental Protection, Huazhong Agricultural University, Wuhan 430070, China"},{"name":"Key Laboratory of Soil Health and Green Remediation, Ministry of Ecology and Environment\/Micro-Element Research Center\/College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiangsheng","family":"Ye","sequence":"additional","affiliation":[{"name":"Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture and Rural Affairs\/State Environmental Protection, Huazhong Agricultural University, Wuhan 430070, China"},{"name":"Key Laboratory of Soil Health and Green Remediation, Ministry of Ecology and Environment\/Micro-Element Research Center\/College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan","family":"Hong","sequence":"additional","affiliation":[{"name":"Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture and Rural Affairs\/State Environmental Protection, Huazhong Agricultural University, Wuhan 430070, China"},{"name":"Key Laboratory of Soil Health and Green Remediation, Ministry of Ecology and Environment\/Micro-Element Research Center\/College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, China"},{"name":"Institute of Environment and Safety, Wuhan Academy of Agricultural Sciences, Wuhan 430070, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3702-5087","authenticated-orcid":false,"given":"Guangda","family":"Ding","sequence":"additional","affiliation":[{"name":"Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture and Rural Affairs\/State Environmental Protection, Huazhong Agricultural University, Wuhan 430070, China"},{"name":"Key Laboratory of Soil Health and Green Remediation, Ministry of Ecology and Environment\/Micro-Element Research Center\/College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,10,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1038\/s41586-018-0656-3","article-title":"Transcriptional regulation of nitrogen-associated metabolism and growth","volume":"563","author":"Gaudinier","year":"2018","journal-title":"Nature"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"105011","DOI":"10.1088\/1748-9326\/9\/10\/105011","article-title":"50 year trends in nitrogen use efficiency of world cropping systems: The relationship between yield and nitrogen input to cropland","volume":"9","author":"Lassaletta","year":"2014","journal-title":"Environ. 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