{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T21:10:53Z","timestamp":1780089053120,"version":"3.54.0"},"reference-count":51,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2021,10,14]],"date-time":"2021-10-14T00:00:00Z","timestamp":1634169600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"I. Ministry of Agriculture of the Czech Republic; II. Ministry of Education, Youth and Sports of the Czech Republic","award":["I. QK1910103; II. CZ.02.1.01\/0.0\/0.0\/16_019\/0000797"],"award-info":[{"award-number":["I. QK1910103; II. CZ.02.1.01\/0.0\/0.0\/16_019\/0000797"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>The antifungal and insecticidal effect of the essential oil from Ocimum sanctum L. was evaluated using a model set of harmful organisms hazardous for health and the economy. Toxigenic and plant pathogenic filamentous fungi, including causal agents of human infections, were chosen as exemplary fungal groups\u2014Fusarium verticillioides, Penicillium expansum and Aspergillus flavus. Spodoptera littoralis (African cotton leafworm), Culex quinquefasciatus (Southern house mosquito), the lymphatic filariasis vector and potential Zika virus vector, and the common housefly, Musca domestica were chosen as model insects. Major and minor active substances were detected and quantified using GC\/MS analysis. Environmental safety was verified using the non-target useful organism Eisenia fetida. Significant antifungal and insecticidal activity, as well as environmental safety, were confirmed. The essential oil showed the highest efficacy against A. flavus according to MIC50\/90, and against S. littoralis larvae according to LD50\/90. The monoterpenoid alcohol linalool, t-methyl cinnamate, and estragole as phenylpropanoids were detected as effective major components (85.4%). The essential oil from Ocimum sanctum L. was evaluated as universal and significantly efficient, providing a high potential for use in environmentally safe botanical pesticides.<\/jats:p>","DOI":"10.3390\/plants10102180","type":"journal-article","created":{"date-parts":[[2021,10,14]],"date-time":"2021-10-14T23:02:16Z","timestamp":1634252536000},"page":"2180","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Antifungal and Insecticidal Potential of the Essential Oil from Ocimum sanctum L. against Dangerous Fungal and Insect Species and Its Safety for Non-Target Useful Soil Species Eisenia fetida (Savigny, 1826)"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2735-9924","authenticated-orcid":false,"given":"Martin","family":"\u017dabka","sequence":"first","affiliation":[{"name":"Crop Research Institute, Drnovsk\u00e1 507, 161 06 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7866-4278","authenticated-orcid":false,"given":"Roman","family":"Pavela","sequence":"additional","affiliation":[{"name":"Crop Research Institute, Drnovsk\u00e1 507, 161 06 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8024-5803","authenticated-orcid":false,"given":"Kate\u0159ina","family":"Kova\u0159\u00edkov\u00e1","sequence":"additional","affiliation":[{"name":"Crop Research Institute, Drnovsk\u00e1 507, 161 06 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"T\u0159\u00edska","sequence":"additional","affiliation":[{"name":"Laboratory of Metabolomics and Isotopic Analyses, Global Change Research Centre, B\u011blidla 986\/4a, 603 00 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nad\u011b\u017eda","family":"Vrchotov\u00e1","sequence":"additional","affiliation":[{"name":"Laboratory of Metabolomics and Isotopic Analyses, Global Change Research Centre, B\u011blidla 986\/4a, 603 00 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Bedn\u00e1\u0159","sequence":"additional","affiliation":[{"name":"Laboratory of Metabolomics and Isotopic Analyses, Global Change Research Centre, B\u011blidla 986\/4a, 603 00 Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,10,14]]},"reference":[{"key":"ref_1","first-page":"240","article-title":"Posological considerations of Ocimum sanctum (Tulasi) as per ayurvedic science and pharmaceutical sciences","volume":"76","author":"Narayana","year":"2014","journal-title":"Indian J. 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