{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T16:57:39Z","timestamp":1790182659213,"version":"4.1.0"},"reference-count":37,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2021,11,20]],"date-time":"2021-11-20T00:00:00Z","timestamp":1637366400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Aspidosperma pyrifolium is used in traditional medicine to treat inflammatory disorders. The aim of the study was to perform phytochemical characterization and evaluate the anti-inflammatory, anti-nociceptive and acute toxicity effects of the total alkaloid fraction (TAF-Ap) from stem barks. Two monoterpenic indole alkaloids were isolated by high performance liquid chromatography coupled with mass spectrometry (HPLC-MS) and the structural elucidation was performed using 1D and 2D NMR analysis. As for toxicity, no animals died at 50 mg kg\u22121 and this concentration presented mild sedation and forced breathing within the first 24 h. The lethal dose capable of killing 50% of the animals (LD50) was estimated to be 160 mg kg\u22121. In the pharmacological tests, the models used were 1% carrageenan-induced paw edema and peritonitis, 1% formalin-induced nociception and 1% acetic acid-induced abdominal writhing in Swiss mice. The study made it possible to isolate 15-methoxyaspidospermine and 15-methoxypyrifolidine, corroborating the results of pharmacological assays, which showed anti-inflammatory and analgesic potential, especially at 30 mg kg\u22121 (p &lt; 0.001). Thus, the species was shown to be a promising source of active substances, with special attention paid to its toxicological potential.<\/jats:p>","DOI":"10.3390\/plants10112526","type":"journal-article","created":{"date-parts":[[2021,11,21]],"date-time":"2021-11-21T21:00:50Z","timestamp":1637528450000},"page":"2526","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Bioguided Isolation of Alkaloids and Pharmacological Effects of the Total Alkaloid Fraction from Aspidosperma pyrifolium Mart. (Apocynaceae)"],"prefix":"10.3390","volume":"10","author":[{"given":"Francisca Sabrina Vieira","family":"Lins","sequence":"first","affiliation":[{"name":"Department of Pharmacy, Post-Graduate Program in Pharmaceutical Sciences, State University of Para\u00edba, Campina Grande 58429-500, PB, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanessa Farias","family":"da Silva","sequence":"additional","affiliation":[{"name":"National Semiarid Institute, Campina Grande 58429-500, PB, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0293-2605","authenticated-orcid":false,"given":"Josean Fechine","family":"Tavares","sequence":"additional","affiliation":[{"name":"Institute for Research in Pharmaceuticals and Medications, Federal University of Para\u00edba, Jo\u00e3o Pessoa 58051-900, PB, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanda L\u00facia","family":"dos Santos","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Post-Graduate Program in Pharmaceutical Sciences, State University of Para\u00edba, Campina Grande 58429-500, PB, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harley","family":"da Silva Alves","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Post-Graduate Program in Pharmaceutical Sciences, State University of Para\u00edba, Campina Grande 58429-500, PB, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,11,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.bpg.2009.11.005","article-title":"Adverse effects of non-steroidal anti- inflammatory drugs (NSAIDs, aspirin and coxibs) on upper gastrointestinal tract","volume":"24","author":"Sostres","year":"2010","journal-title":"J. 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