{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,18]],"date-time":"2026-07-18T06:25:58Z","timestamp":1784355958618,"version":"3.55.0"},"reference-count":64,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2021,11,29]],"date-time":"2021-11-29T00:00:00Z","timestamp":1638144000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Kurchatov Genomics Center of All-Russia Research Institute of Agricultural Biotechnology","award":["#075-15-2019-1667"],"award-info":[{"award-number":["#075-15-2019-1667"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Triticum timopheevii Zhuk. is a tetraploid wheat that is utilized worldwide as a valuable breeding source for wheat improvement. Gene-based biotechnologies can contribute to this field; however, T. timopheevii exhibits recalcitrance and albinism in tissue cultures, making this species of little use for manipulation through genetic engineering and genome editing. This study tested various approaches to increasing in vitro somatic embryogenesis and plant regeneration, while reducing the portion of albinos in cultures derived from immature embryos (IEs) of T. timopheevii. They included (i) adjusting the balance between 2,4-D and daminozide in callus induction medium; (ii) cultivation using various darkness\/illumination schedules; and (iii) inclusion of additional concentrations of copper ions in the tissue culture medium. We achieved a 2.5-fold increase in somatic embryogenesis (up to 80%) when 50 mg L\u22121 daminozide was included in the callus induction medium together with 3 mg L\u22121 2,4-D. It was found that the dark cultivation for 20\u201330 days was superior in terms of achieving maximum culture efficiency; moreover, switching to light in under 2 weeks from culture initiation significantly increased the number of albino plants, suppressed somatic embryogenesis, and decreased the regeneration of green plants. Media containing higher levels of copper ions did not have a positive effect on the regeneration of green plants; contrarily, the elevated concentrations caused albinism in plantlets. The results and relevant conclusions of the present study might be valuable for establishing an improved protocol for the regeneration of green plants in tissue cultures of T. timopheevii.<\/jats:p>","DOI":"10.3390\/plants10122620","type":"journal-article","created":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T02:34:05Z","timestamp":1638326045000},"page":"2620","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["The Effect of Daminozide, Dark\/Light Schedule and Copper Sulphate in Tissue Culture of Triticum timopheevii"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3975-7484","authenticated-orcid":false,"given":"Dmitry","family":"Miroshnichenko","sequence":"first","affiliation":[{"name":"Kurchatov Genomics Center of All-Russia Research Institute of Agricultural Biotechnology, Timiryazevskaya Street 42, 127550 Moscow, Russia"},{"name":"Branch of Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russion Academy of Science, 142290 Pushchino, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Klementyeva","sequence":"additional","affiliation":[{"name":"Branch of Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russion Academy of Science, 142290 Pushchino, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergey","family":"Dolgov","sequence":"additional","affiliation":[{"name":"Branch of Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russion Academy of Science, 142290 Pushchino, Russia"},{"name":"All-Russia Research Institute of Agricultural Biotechnology, Timiryazevskaya Street 42, 127550 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,11,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Devi, U., Grewal, S., Yang, C.-Y., Hubbart-Edwards, S., Scholefield, D., Ashling, S., Burridge, A., King, I.P., and King, J. 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