{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T15:53:40Z","timestamp":1792166020585,"version":"4.4.0"},"reference-count":35,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2021,12,2]],"date-time":"2021-12-02T00:00:00Z","timestamp":1638403200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>The Tunisian Ziziphus lotus plant was investigated to determine its phytoconstituents and evaluate its biological activities. In particular, the GC\/MS technique was used to describe the chemical composition of Z. lotus active extracts and fractions. Among the obtained extracts, the yields of the dried root methanolic extract (29.80%) and the fruit aqueous extract (48.00%) were the highest ones. The dried root methanolic extract exhibited the highest amount in the total phenolics (186.44 \u00b1 0.26 mg GAE\/g DW), total flavonoids (102.50 \u00b1 3.53 mg QE\/g DW), and tannins (60.714 \u00b1 2.2 mg catechin\/g DW). The root aqueous extracts revealed the highest antioxidant activity with an IC50 of 8.96 \u00b1 0.38 mg\/L and 16.46 \u00b1 0.60 mg\/L for the ABTS\u2022+ and DPPH\u2022 assays, respectively. The total antioxidant capacity was accorded to the methanolic extract of the dried roots with a value of 304.07 \u00b1 1.11 \u00b5g AAE\/mg. The drying process was found to improve the qualitative and quantitative properties of the Z. lotus extracts. The evaluation of the cytotoxic activity against the SH-SY5Y cell line was carried out using MTT assay. The petroleum ether and dichloromethane extracts of the dried roots showed relevant cytotoxic activities. The thin layer chromatography and the GC-MS\/GC-FID analysis led to the identification of the 13-epimanool as a potent cytotoxic compound.<\/jats:p>","DOI":"10.3390\/plants10122651","type":"journal-article","created":{"date-parts":[[2021,12,2]],"date-time":"2021-12-02T21:19:08Z","timestamp":1638479948000},"page":"2651","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Chemical Composition and Biological Activities of Tunisian Ziziphus lotus Extracts: Evaluation of Drying Effect, Solvent Extraction, and Extracted Plant Parts"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6124-0639","authenticated-orcid":false,"given":"Touka","family":"Letaief","sequence":"first","affiliation":[{"name":"Laboratory of Materials Molecules and Applications (LMMA), IPEST, University of Carthage, BP 51, La Marsa 2070, Tunisia"},{"name":"National Agronomic Institute of Tunisia (INAT), University of Carthage, Mahrajene 1082, Tunisia"},{"name":"Department for the Innovation in Biological, Agrofood and Forestal Systems, Tuscia University, 01100 Viterbo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8535-0533","authenticated-orcid":false,"given":"Stefania","family":"Garzoli","sequence":"additional","affiliation":[{"name":"Department of Drug Chemistry and Technology, Sapienza University, 00185 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7680-7404","authenticated-orcid":false,"given":"Valentina","family":"Laghezza Masci","sequence":"additional","affiliation":[{"name":"Department for the Innovation in Biological, Agrofood and Forestal Systems, Tuscia University, 01100 Viterbo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0533-2285","authenticated-orcid":false,"given":"Jamel","family":"Mejri","sequence":"additional","affiliation":[{"name":"Laboratory of Materials Molecules and Applications (LMMA), IPEST, University of Carthage, BP 51, La Marsa 2070, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manef","family":"Abderrabba","sequence":"additional","affiliation":[{"name":"Laboratory of Materials Molecules and Applications (LMMA), IPEST, University of Carthage, BP 51, La Marsa 2070, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Tiezzi","sequence":"additional","affiliation":[{"name":"Department for the Innovation in Biological, Agrofood and Forestal Systems, Tuscia University, 01100 Viterbo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8876-6331","authenticated-orcid":false,"given":"Elisa","family":"Ovidi","sequence":"additional","affiliation":[{"name":"Department for the Innovation in Biological, Agrofood and Forestal Systems, Tuscia University, 01100 Viterbo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,12,2]]},"reference":[{"key":"ref_1","first-page":"494","article-title":"Rekha Vijayvergia Bioactivity of secondary metabolites of various plants: A review","volume":"10","author":"Jain","year":"2019","journal-title":"Int. J. Pharm. Sci. Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1000","DOI":"10.21931\/RB\/2019.04.04.11","article-title":"Secondary metabolites in plants: Main classes, phytochemical analysis and pharmacological activities","volume":"4","author":"Mera","year":"2019","journal-title":"Rev. Bionatura"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.4103\/jpbs.JPBS_175_19","article-title":"Abubakar and Mainul Haque Preparation of Medicinal Plants: Basic Extraction and Fractionation Procedures for Experimental Purposes","volume":"12","author":"Abdullahi","year":"2020","journal-title":"J. Pharm. Bioallied Sci."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Altemimi, A., Lakhssassi, N., Baharlouei, A., Watson, D.G., and Lightfoot, D.A. (2017). Phytochemicals: Extraction, isolation, and identification of bioactive compounds from plant extracts. Plants, 6.","DOI":"10.3390\/plants6040042"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Yonbawi, A.R., Abdallah, H.M., Alkhilaiwi, F.A., Koshak, A.E., and Heard, C.M. (2021). Anti-Proliferative, Cytotoxic and Antioxidant Properties of the Methanolic Extracts of Five Saudi Arabian Flora with Folkloric Medicinal Use: Aizoon canariense, Citrullus colocynthis, Maerua crassifolia, Rhazya stricta and Tribulus macropterus. Plants, 10.","DOI":"10.3390\/plants10102073"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1007\/s10298-013-0776-8","article-title":"Zizyphus lotus (L.) Desf. (Rhamnaceae): Jujubier sauvage","volume":"11","author":"Ghedira","year":"2013","journal-title":"Phytotherapie"},{"key":"ref_7","first-page":"2898","article-title":"Mineral composition of leaves and fruits of some promising jujube (zizyphus jujuba miller) genotypes","volume":"21","author":"San","year":"2009","journal-title":"Asian J. Chem."},{"key":"ref_8","first-page":"63","article-title":"The influence of water-deficit stress on growth, water relations and solute accumulation in wild Jujube","volume":"1","author":"Maraghni","year":"2011","journal-title":"J. Ornam. Hortic. Plants"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Guirado, E., Tabik, S., Alcaraz-Segura, D., Cabello, J., and Herrera, F. (2017). Deep-learning Versus OBIA for scattered shrub detection with Google Earth Imagery: Ziziphus lotus as case study. Remote Sens., 9.","DOI":"10.3390\/rs9121220"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1016\/j.sajb.2010.02.092","article-title":"Seed germination at different temperatures and water stress levels, and seedling emergence from different depths of Ziziphus lotus","volume":"76","author":"Maraghni","year":"2010","journal-title":"S. Afr. J. Bot."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/j.foodchem.2015.03.047","article-title":"Optimization of ultrasound-assisted extraction of antioxidant compounds from Tunisian Zizyphus lotus fruits using response surface methodology","volume":"184","author":"Hammi","year":"2015","journal-title":"Food Chem."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1080\/15324982.2018.1424742","article-title":"Genetic diversity of Ziziphus lotus natural populations from Algeria based on fruit morphological markers","volume":"32","author":"Boussaid","year":"2018","journal-title":"Arid L. Res. Manag."},{"key":"ref_13","first-page":"s1","article-title":"Determination of phenol content and evaluation of in vitro litholytic effects on urolithiasis of moroccan Zizyphus lotus L. extract","volume":"16","author":"Khouchlaa","year":"2020","journal-title":"Phytotherapie"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1016\/j.jep.2007.02.024","article-title":"Antiulcerogenic activity of Zizyphus lotus (L.) extracts","volume":"112","author":"Borgi","year":"2007","journal-title":"J. Ethnopharmacol."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Benammar, C., Hichami, A., Yessoufou, A., Simonin, A.M., Belarbi, M., Allali, H., and Khan, N.A. (2010). Zizyphus lotus L. (Desf.) modulates antioxidant activity and human T-cell proliferation. BMC Complement. Altern. Med., 10.","DOI":"10.1186\/1472-6882-10-54"},{"key":"ref_16","first-page":"410","article-title":"\u00c9valuation des activit\u00e9s antifongique et molluscicide de Zizyphus lotus (L.) Desf. du Maroc","volume":"60","author":"Lahlou","year":"2002","journal-title":"Ann. Pharm. fran\u00e7aises"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1591","DOI":"10.1016\/0031-9422(93)85186-U","article-title":"Le Two cyclopeptide alkaloids from Zizyphus lotus","volume":"32","author":"Ghedira","year":"1993","journal-title":"Phytochemistry"},{"key":"ref_18","first-page":"429","article-title":"Methyl carnosate, an antibacterial diterpene isolated from salvia officinalis leaves","volume":"8","author":"Climati","year":"2013","journal-title":"Nat. Prod. Commun."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"5990","DOI":"10.1039\/c3ay41125g","article-title":"The Folin-Ciocalteu assay revisited: Improvement of its specificity for total phenolic content determination","volume":"5","author":"Benavides","year":"2013","journal-title":"Anal. Methods"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"32","DOI":"10.5530\/pj.2014.4.5","article-title":"Study on the antioxidant activities of root extracts of Zizyphus lotus from the western region of Algeria","volume":"6","author":"Ghalem","year":"2014","journal-title":"Pharmacogn. J."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"18","DOI":"10.21448\/ijsm.275886","article-title":"Quantification of total phenols, flavonoides and tannins from Ziziphus jujuba (mill.) and Ziziphus lotus (l.) (Desf). Leaf extracts and their effects on antioxidant and antibacterial activities","volume":"4","author":"Elaloui","year":"2016","journal-title":"Int. J. Second. Metab."},{"key":"ref_22","first-page":"316","article-title":"Phenolic Compounds, Antioxidant and Antibacterial Activities of Zizyphus lotus L. Leaves Extracts","volume":"7","author":"Abderrahim","year":"2017","journal-title":"Nat. Prod. J."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Olajuyigbe, O.O., and Afolayan, A.J. (2011). Phenolic content and antioxidant property of the bark extracts of Ziziphus mucronata Willd. subsp. mucronata Willd. BMC Complement. Altern. Med., 11.","DOI":"10.1186\/1472-6882-11-130"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1080\/07373930802683740","article-title":"Drying of lemon myrtle (Backhousia citriodora) leaves: Retention of volatiles and color","volume":"27","author":"Buchaillot","year":"2009","journal-title":"Dry. Technol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.indcrop.2016.01.043","article-title":"Effect of air-drying temperature on extractable and non-extractable phenolics and antioxidant capacity of lime wastes","volume":"84","year":"2016","journal-title":"Ind. Crops Prod."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1258","DOI":"10.1080\/00032719.2015.1082134","article-title":"Teucrium ramosissimum (Lamiaceae): Volatile Composition, Seasonal Variation, and Pharmaceutical Activity","volume":"49","author":"Ghazouani","year":"2016","journal-title":"Anal. Lett."},{"key":"ref_27","first-page":"1","article-title":"Phytochemical Analysis and Antioxidant Activities of Dry and Fresh Leaves of Petivera alliacea and Ocimum","volume":"24","author":"Olufunmilayo","year":"2015","journal-title":"Int. J. Sci. Basic Appl. Res."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"El Cadi, H., Bouzidi, H.E.L., Selama, G., El Cadi, A., Ramdan, B., Oulad, Y., Majdoub, E., Alibrando, F., Dugo, P., and Mondello, L. (2020). Physico-Chemical and Phytochemical Characterization of Moroccan Wild Jujube \u201cZizyphus lotus (L.)\u201d Fruit Crude Extract and Fractions. Molecules, 25.","DOI":"10.3390\/molecules25225237"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"20","DOI":"10.3923\/ajcb.2017.20.27","article-title":"Cytotoxic Potential of N-hexadecanoic Acid Extracted from Kigelia pinnata Leaves","volume":"12","author":"Ravi","year":"2016","journal-title":"Asian J. Cell Biol."},{"key":"ref_30","first-page":"2587","article-title":"Antitumor activity of palmitic acid found as a selective cytotoxic substance in a marine red alga","volume":"22","author":"Harada","year":"2002","journal-title":"Anticancer Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"5898","DOI":"10.1039\/C9FO01423C","article-title":"HPLC-DAD-ESI-MS\/MS screening of phytochemical compounds and the bioactive properties of different plant parts of: Zizyphus lotus (L.) Desf","volume":"10","author":"Rached","year":"2019","journal-title":"Food Funct."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"338","DOI":"10.3892\/ijo.2012.1690","article-title":"The dichloromethane extract of the ethnomedicinal plant Neurolaena lobata inhibits NPM\/ALK expression which is causal for anaplastic large cell lymphomagenesis","volume":"42","author":"Christine","year":"2013","journal-title":"Int. J. Oncol."},{"key":"ref_33","first-page":"1551","article-title":"Experimental design methodologyapplication in the optimization of phytochemical compoundsextraction and antioxidant activity of Thymelaea hirsuta L. extracts","volume":"9","author":"Yahyaoui","year":"2018","journal-title":"J. Mater. Environ. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/j.foodchem.2014.03.030","article-title":"Phytochemicals and antioxidant activities of Rhus tripartitum (Ucria) fruits depending on locality and different stages of maturity","volume":"160","author":"Tlili","year":"2014","journal-title":"Food Chem."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1923","DOI":"10.1080\/14786419.2019.1644628","article-title":"GC-MS investigation and antiproliferative activities of extracts from male and female flowers of Schinus molle L","volume":"35","author":"Ovidi","year":"2021","journal-title":"Nat. Prod. Res."}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/10\/12\/2651\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T07:38:56Z","timestamp":1760168336000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/10\/12\/2651"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,12,2]]},"references-count":35,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2021,12]]}},"alternative-id":["plants10122651"],"URL":"https:\/\/doi.org\/10.3390\/plants10122651","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,12,2]]}}}