{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T14:04:54Z","timestamp":1780495494102,"version":"3.54.1"},"reference-count":57,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2021,12,16]],"date-time":"2021-12-16T00:00:00Z","timestamp":1639612800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>The Yaqui Valley, Mexico, has been historically considered as an experimental field for semiarid regions worldwide since temperature is an important constraint affecting durum wheat cultivation. Here, we studied the transcriptional and morphometrical response of durum wheat at an increased temperature (+2 \u00b0C) for deciphering molecular mechanisms involved in the thermal adaptation by this crop. The morphometrical assay showed a significant decrease in almost all the evaluated traits (shoot\/root length, biovolume index, and dry\/shoot weight) except in the dry root weight and the root:shoot ratio. At the transcriptional level, 283 differentially expressed genes (DEGs) were obtained (False Discovery Rate (FDR) \u2264 0.05 and |log2 fold change| \u2265 1.3). From these, functional annotation with MapMan4 and a gene ontology (GO) enrichment analysis with GOSeq were carried out to obtain 27 GO terms significantly enriched (overrepresented FDR \u2264 0.05). Overrepresented and functionally annotated genes belonged to ontologies associated with photosynthetic acclimation, respiration, changes in carbon balance, lipid biosynthesis, the regulation of reactive oxygen species, and the acceleration of physiological progression. These findings are the first insight into the regulation of the mechanism influenced by a temperature increase in durum wheat.<\/jats:p>","DOI":"10.3390\/plants10122792","type":"journal-article","created":{"date-parts":[[2021,12,16]],"date-time":"2021-12-16T21:32:40Z","timestamp":1639690360000},"page":"2792","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Transcriptional Regulation of Metabolic and Cellular Processes in Durum Wheat (Triticum turgidum subsp. durum) in the Face of Temperature Increasing"],"prefix":"10.3390","volume":"10","author":[{"given":"Luis Abraham","family":"Chaparro-Encinas","sequence":"first","affiliation":[{"name":"Instituto Tecnol\u00f3gico de Sonora, 5 de Febrero 818 Sur, Ciudad Obreg\u00f3n 85000, Sonora, Mexico"},{"name":"Departamento de Fitomejoramiento, Universidad Aut\u00f3noma Agraria Antonio Narro (UAAAN) Unidad Laguna, Perif\u00e9rico Ra\u00fal L\u00f3pez S\u00e1nchez, Valle Verde, Torre\u00f3n 27054, Coahuila, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0374-9661","authenticated-orcid":false,"given":"Gustavo","family":"Santoyo","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Qu\u00edmico Biol\u00f3gicas, Universidad Michoacana de San Nicol\u00e1s de Hidalgo, Morelia 58000, Michoac\u00e1n, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan Jos\u00e9","family":"Pe\u00f1a-Cabriales","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n y de Estudios Avanzados, Unidad Irapuato, Libramiento Norte Carretera Irapuato Le\u00f3n Kil\u00f3metro 9.6, Carr Panamericana Irapuato Le\u00f3n, Irapuato 36821, Guanajuato, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciano","family":"Castro-Espinoza","sequence":"additional","affiliation":[{"name":"Instituto Tecnol\u00f3gico de Sonora, 5 de Febrero 818 Sur, Ciudad Obreg\u00f3n 85000, Sonora, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fannie Isela","family":"Parra-Cota","sequence":"additional","affiliation":[{"name":"Campo Experimental Norman E. Borlaug, Instituto Nacional de Investigaciones Forestales, Agr\u00edcolas y Pecuarias (INIFAP), Norman E. Borlaug Km. 12, Valle del Yaqui, Ciudad Obreg\u00f3n 85000, Sonora, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2234-7147","authenticated-orcid":false,"given":"Sergio de los","family":"Santos-Villalobos","sequence":"additional","affiliation":[{"name":"Instituto Tecnol\u00f3gico de Sonora, 5 de Febrero 818 Sur, Ciudad Obreg\u00f3n 85000, Sonora, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,12,16]]},"reference":[{"key":"ref_1","unstructured":"SIAP (2020). Panorama Agroalimentario 2020, Secretaria de Agricultura y Desarrollo Rural. [2020th ed.]."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Matson, P., and Jewett, P. (2013). Ecosystems and land-use change in the Yaqui Valley: Does agricultural intensification spare land for nature?. Seeds of Sustainability: Lessons from the Birthplace of the Green Revolution, Island Press\/Center for Resource Economics.","DOI":"10.5822\/978-1-61091-177-1_4"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.agee.2018.04.003","article-title":"Nitrous oxide (N2O) flux responds exponentially to nitrogen fertilizer in irrigated wheat in the Yaqui Valley, Mexico","volume":"261","author":"Millar","year":"2018","journal-title":"Agric. Ecosyst. Environ."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1219","DOI":"10.1016\/j.tibtech.2018.07.008","article-title":"Towards Food Security: Current State and Future Prospects of Agrobiotechnology","volume":"36","author":"Tyczewska","year":"2018","journal-title":"Trends Biotechnol."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Ray, D.K., Mueller, N.D., West, P.C., and Foley, J.A. (2013). Yield Trends Are Insufficient to Double Global Crop Production by 2050. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0066428"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.fcr.2017.12.015","article-title":"Quantifying the response of wheat yields to heat stress: The role of the experimental setup","volume":"217","author":"Rezaei","year":"2018","journal-title":"Field Crop. Res."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1130","DOI":"10.1038\/nclimate3115","article-title":"Similar estimates of temperature impacts on global wheat yield by three independent methods","volume":"6","author":"Liu","year":"2016","journal-title":"Nat. Clim. Chang."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/nplants.2015.190","article-title":"Molecular and genetic control of plant thermomorphogenesis","volume":"2","author":"Quint","year":"2016","journal-title":"Nat. Plants"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2018","DOI":"10.1111\/pce.13979","article-title":"Hot topic: Thermosensing in plants","volume":"44","author":"Hayes","year":"2021","journal-title":"Plant Cell Environ."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Sharma, D., Singh, R., Tiwari, R., Kumar, R., and Gupta, V.K. (2019). Wheat Responses and Tolerance to Terminal Heat Stress: A Review. Wheat Production in Changing Environments, Springer.","DOI":"10.1007\/978-981-13-6883-7_7"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Balla, K., Karsai, I., B\u00f3nis, P., Kiss, T., Berki, Z., Horv\u00e1th, \u00c1., Mayer, M., Bencze, S., and Veisz, O. (2019). Heat stress responses in a large set of winter wheat cultivars (Triticum aestivum L.) depend on the timing and duration of stress. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0222639"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/j.eja.2014.10.003","article-title":"Heat stress in cereals: Mechanisms and modelling","volume":"64","author":"Webber","year":"2015","journal-title":"Eur. J. Agron."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.cj.2017.09.005","article-title":"Genetic improvement of heat tolerance in wheat: Recent progress in understanding the underlying molecular mechanisms","volume":"6","author":"Ni","year":"2018","journal-title":"Crop J."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1890","DOI":"10.1111\/gcb.13212","article-title":"Testing the responses of four wheat crop models to heat stress at anthesis and grain filling","volume":"22","author":"Liu","year":"2016","journal-title":"Glob. Chang. Biol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1016\/j.devcel.2020.10.012","article-title":"Thriving under Stress: How Plants Balance Growth and the Stress Response","volume":"55","author":"Zhang","year":"2020","journal-title":"Dev. Cell"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1104\/pp.18.01377","article-title":"A mobile auxin signal connects temperature sensing in cotyledons with growth responses in hypocotyls","volume":"180","author":"Bellstaedt","year":"2019","journal-title":"Plant Physiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1624","DOI":"10.1104\/pp.19.01464","article-title":"How Plants Sense and Respond to Stressful Environments","volume":"182","author":"Lamers","year":"2020","journal-title":"Plant Physiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1471-2199-7-3","article-title":"The RIN: An RNA integrity number for assigning integrity values to RNA measurements","volume":"7","author":"Schroeder","year":"2006","journal-title":"BMC Mol. Biol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"879","DOI":"10.1016\/j.molp.2019.01.003","article-title":"MapMan4: A Refined Protein Classification and Annotation Framework Applicable to Multi-Omics Data Analysis","volume":"12","author":"Schwacke","year":"2019","journal-title":"Mol. Plant"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"R14","DOI":"10.1186\/gb-2010-11-2-r14","article-title":"Gene ontology analysis for RNA-seq: Accounting for selection bias GOseq GOseq is a method for GO analysis of RNA-seq data that takes into account the length bias inherent in RNA-seq","volume":"11","author":"Young","year":"2010","journal-title":"Genome Biol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1016\/j.wace.2015.08.001","article-title":"Temperature extremes: Effect on plant growth and development","volume":"10","author":"Hatfield","year":"2015","journal-title":"Weather Clim. Extrem."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"7143","DOI":"10.1002\/ece3.4238","article-title":"Root:shoot ratio in developing seedlings: How seedlings change their allocation in response to seed mass and ambient nutrient supply","volume":"8","author":"Herben","year":"2018","journal-title":"Ecol. Evol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"plv101","DOI":"10.1093\/aobpla\/plv101","article-title":"Natural populations of Arabidopsis thaliana differ in seedling responses to high temperature stress","volume":"7","author":"Zhang","year":"2015","journal-title":"AoB Plants"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1038\/s41467-019-08398-5","article-title":"Continuous dynamic adjustment of the plant circadian oscillator","volume":"10","author":"Webb","year":"2019","journal-title":"Nat. Commun."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1215","DOI":"10.1111\/nph.15518","article-title":"Thermal adaptation and plasticity of the plant circadian clock","volume":"221","author":"Gil","year":"2019","journal-title":"New Phytol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fpls.2020.614360","article-title":"Time Lag Between Light and Heat Diurnal Cycles Modulates CIRCADIAN CLOCK ASSOCIATION 1 Rhythm and Growth in Arabidopsis thaliana","volume":"11","author":"Masuda","year":"2021","journal-title":"Front. Plant Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"e0121658","DOI":"10.1371\/journal.pone.0121658","article-title":"The thylakoid membrane protein CGL160 supports CF1CF0 ATP synthase accumulation in Arabidopsis thaliana","volume":"10","author":"Fristedt","year":"2015","journal-title":"PLoS ONE"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"3646","DOI":"10.1105\/tpc.114.127373","article-title":"The Light-Harvesting Chlorophyll a\/b Binding Proteins Lhcb1 and Lhcb2 Play Complementary Roles during State Transitions in Arabidopsis","volume":"26","author":"Pietrzykowska","year":"2014","journal-title":"Plant Cell"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1007\/s00709-011-0357-3","article-title":"The novel functions of kinesin motor proteins in plants","volume":"249","author":"Li","year":"2012","journal-title":"Protoplasma"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"885","DOI":"10.1016\/j.molp.2016.03.002","article-title":"The Arabidopsis Protein CGLD11 Is Required for Chloroplast ATP Synthase Accumulation","volume":"9","author":"Grahl","year":"2016","journal-title":"Mol. Plant"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fpls.2017.01732","article-title":"ALA6, a P4-type ATPase, is involved in heat stress responses in Arabidopsis thaliana","volume":"8","author":"Niu","year":"2017","journal-title":"Front. Plant Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1104\/pp.16.00355","article-title":"Photosynthesis activates plasma membrane H+-ATPase via sugar accumulation","volume":"171","author":"Okumura","year":"2016","journal-title":"Plant Physiol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"3765","DOI":"10.1093\/jxb\/erz532","article-title":"Molecular bases of responses to abiotic stress in trees","volume":"71","author":"Mattera","year":"2020","journal-title":"J. Exp. Bot."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12870-015-0511-8","article-title":"Temporal transcriptome profiling reveals expression partitioning of homeologous genes contributing to heat and drought acclimation in wheat (Triticum aestivum L.)","volume":"15","author":"Liu","year":"2015","journal-title":"BMC Plant Biol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1104\/pp.17.00861","article-title":"Phospholipid: Diacylglycerol acyltransferase-mediated triacylglyerol synthesis augments basal thermotolerance","volume":"175","author":"Mueller","year":"2017","journal-title":"Plant Physiol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fpls.2020.611897","article-title":"Phospholipid:Diacylglycerol Acyltransferase1 Overexpression Delays Senescence and Enhances Post-heat and Cold Exposure Fitness","volume":"11","author":"Demski","year":"2020","journal-title":"Front. Plant Sci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"856","DOI":"10.1111\/tpj.13299","article-title":"Reactive oxygen species, abiotic stress and stress combination","volume":"90","author":"Choudhury","year":"2017","journal-title":"Plant J."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1016\/j.plaphy.2019.04.039","article-title":"Acquiring control: The evolution of ROS-Induced oxidative stress and redox signaling pathways in plant stress responses","volume":"141","author":"Farooq","year":"2019","journal-title":"Plant Physiol. Biochem."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1673","DOI":"10.1016\/S2095-3119(14)60980-1","article-title":"The cytochrome P450 superfamily: Key players in plant development and defense","volume":"14","author":"Xu","year":"2015","journal-title":"J. Integr. Agric."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12864-019-5799-6","article-title":"Microrna-guided regulation of heat stress response in wheat","volume":"20","author":"Ravichandran","year":"2019","journal-title":"BMC Genom."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fgene.2021.699420","article-title":"Discovery of miRNAs and Development of Heat-Responsive miRNA-SSR Markers for Characterization of Wheat Germplasm for Terminal Heat Tolerance Breeding","volume":"12","author":"Sihag","year":"2021","journal-title":"Front. Genet."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Zhao, J., Lu, Z., Wang, L., and Jin, B. (2021). Plant responses to heat stress: Physiology, transcription, noncoding rnas, and epigenetics. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22010117"},{"key":"ref_43","first-page":"745","article-title":"Cirno c2008, nueva variedad de trigo cristalino con alto rendimiento potencial para el estado de Sonora","volume":"1","year":"2010","journal-title":"Rev. Mex. Ciencias Agr\u00edcolas"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"229","DOI":"10.29312\/remexca.v11i1.2162","article-title":"Mejorando rasgos biom\u00e9tricos de pl\u00e1ntulas de trigo con la inoculaci\u00f3n de un consorcio nativo de Bacillus","volume":"11","year":"2020","journal-title":"Rev. Mex. Ciencias Agr\u00edcolas"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.chemosphere.2018.06.073","article-title":"Soil sterilisation methods for use in OECD 106: How effective are they?","volume":"209","author":"Lees","year":"2018","journal-title":"Chemosphere"},{"key":"ref_46","unstructured":"Verhulst, N., Deckers, J., and Govaerts, B. (2009). Classification of the Soil at CIMMYT \u2019s Experimental Station in the Yaqui Valley Near Ciudad Obreg\u00f3n, Sonora, M\u00e9xico, CIMMYT. CIMMYT Report."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1007\/s11104-018-03901-1","article-title":"Plant-assisted selection: A promising alternative for in vivo identification of wheat (Triticum turgidum L. subsp. Durum) growth promoting bacteria","volume":"435","author":"Santoyo","year":"2019","journal-title":"Plant Soil"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1111\/j.1365-3180.1974.tb01084.x","article-title":"A Decimal Code for the Growth Stages of Cereals","volume":"14","author":"Zadoks","year":"1974","journal-title":"Weed Res."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Rojas Padilla, J., Chaparro Encinas, L.A., Robles Montoya, R.I., and De los Santos Villalobos, S. (2020). Promoci\u00f3n de crecimiento en trigo (Triticum turgidum L. subsp. durum) por la co-inoculaci\u00f3n de cepas nativas de Bacillus aisladas del Valle del Yaqui, M\u00e9xico. Nov. Sci., 12.","DOI":"10.21640\/ns.v12i24.2136"},{"key":"ref_50","first-page":"247","article-title":"Techniques for nucleic acid engineering: The foundation of gene manipulation","volume":"Volume 1","author":"Baskin","year":"2018","journal-title":"Omics Technologies and Bio-Engineering: Towards Improving Quality of Life"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1007\/s42976-020-00046-9","article-title":"A modified CTAB and Trizol\u00ae protocol for high-quality RNA extraction from whole wheat seedlings, including rhizosphere","volume":"48","year":"2020","journal-title":"Cereal Res. Commun."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"2114","DOI":"10.1093\/bioinformatics\/btu170","article-title":"Trimmomatic: A flexible trimmer for Illumina sequence data","volume":"30","author":"Bolger","year":"2014","journal-title":"Bioinformatics"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1126\/science.aan0032","article-title":"Wild emmer genome architecture and diversity elucidate wheat evolution and domestication","volume":"357","author":"Avni","year":"2017","journal-title":"Science"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nmeth.3317","article-title":"HISAT: A fast spliced aligner with low memory requirements","volume":"12","author":"Kim","year":"2015","journal-title":"Nat. Methods"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1093\/bioinformatics\/btu638","article-title":"HTSeq-A Python framework to work with high-throughput sequencing data","volume":"31","author":"Anders","year":"2015","journal-title":"Bioinformatics"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2","volume":"15","author":"Love","year":"2014","journal-title":"Genome Biol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"W589","DOI":"10.1093\/nar\/gkv350","article-title":"The BioMart community portal: An innovative alternative to large, centralized data repositories","volume":"43","author":"Smedley","year":"2015","journal-title":"Nucleic Acids Res."}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/10\/12\/2792\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T07:50:16Z","timestamp":1760169016000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/10\/12\/2792"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,12,16]]},"references-count":57,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2021,12]]}},"alternative-id":["plants10122792"],"URL":"https:\/\/doi.org\/10.3390\/plants10122792","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,12,16]]}}}