{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T20:27:57Z","timestamp":1785529677988,"version":"3.56.0"},"reference-count":52,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2021,12,18]],"date-time":"2021-12-18T00:00:00Z","timestamp":1639785600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006595","name":"Unitatea Executiva Pentru Finantarea Invatamantului Superior a Cercetarii Dezvoltarii si Inovarii","doi-asserted-by":"publisher","award":["PN-III-P1-1.1-PD-2019-0967"],"award-info":[{"award-number":["PN-III-P1-1.1-PD-2019-0967"]}],"id":[{"id":"10.13039\/501100006595","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>The present study aimed to compare two polyphenolic-enriched extracts obtained from the Thymus marschallianus Willd. (Lamiaceae) species, harvested from culture (TMCE in doses of 0.66 \u03bcg GAE\/mL and 0.066 \u03bcg GAE\/mL) and from spontaneous flora (TMSE in doses of 0.94 \u03bcg GAE\/mL and 0.094 \u03bcg GAE\/mL) by assessing their biological effects on human umbilical vein endothelial cells (HUVECs) exposed to normoglycemic (137 mmol\/L glucose) and hyperglycemic conditions (200 mmol\/L glucose). Extracts were obtained by solid phase extraction (SPE) and analyzed by chromatographical (HPLC-DAD) and spectrophotometrical methods. Their effects on hyperglycemia were evaluated by the quantification of oxidative stress and NF-\u0138B, pNF-\u0138B, HIF-1\u03b1, and \u03b3-H2AX expressions. The HPLC-DAD analysis highlighted significant amounts of rosmarinic acid (ranging between 0.18 and 1.81 mg\/g dry extract), luteolin (ranging between 2.04 and 17.71 mg\/g dry extract), kaempferol (ranging between 1.85 and 7.39 mg\/g dry extract), and apigenin (ranging between 4.97 and 65.67 mg\/g dry extract). Exposure to hyperglycemia induced oxidative stress and the activation of NF-\u0138 increased the expression of HIF-1\u03b1 and produced DNA lesions. The polyphenolic-enriched extracts proved a significant reduction of oxidative stress and \u03b3-H2AX formation and improved the expression of HIF-1\u03b1, suggesting their protective role on endothelial cells in hyperglycemia. The tested extracts reduced the total NF-\u0138B expression and diminished its activation in hyperglycemic conditions. The obtained results bring evidence for the use of the polyphenolic-enriched extracts of T. marschallianus as adjuvants in hyperglycemia.<\/jats:p>","DOI":"10.3390\/plants10122810","type":"journal-article","created":{"date-parts":[[2021,12,20]],"date-time":"2021-12-20T02:50:18Z","timestamp":1639968618000},"page":"2810","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Oxidative Stress and DNA Lesion Reduction of a Polyphenolic Enriched Extract of Thymus marschallianus Willd. in Endothelial Vascular Cells Exposed to Hyperglycemia"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4098-6962","authenticated-orcid":false,"given":"Irina","family":"Ielciu","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Botany, Faculty of Pharmacy, \u201cIuliu Ha\u0163ieganu\u201d University of Medicine and Pharmacy, 400010 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7447-4925","authenticated-orcid":false,"given":"Gabriela Adriana","family":"Filip","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Medicine, \u201cIuliu Ha\u0163ieganu\u201d University of Medicine and Pharmacy, 400006 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7660-9993","authenticated-orcid":false,"given":"Ilioara","family":"Oniga","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy, \u201cIuliu Ha\u0163ieganu\u201d University of Medicine and Pharmacy, 400010 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5204-6885","authenticated-orcid":false,"given":"Neli-Kinga","family":"Olah","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry, Faculty of Pharmacy, \u201cVasile Goldi\u015f\u201d Western University of Arad, 310414 Arad, Romania"},{"name":"PlantExtrakt Ltd., R\u0103daia, 407059 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ioana","family":"B\u00e2ldea","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Medicine, \u201cIuliu Ha\u0163ieganu\u201d University of Medicine and Pharmacy, 400006 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana","family":"Olteanu","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Medicine, \u201cIuliu Ha\u0163ieganu\u201d University of Medicine and Pharmacy, 400006 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramona Flavia","family":"Burtescu","sequence":"additional","affiliation":[{"name":"PlantExtrakt Ltd., R\u0103daia, 407059 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Violeta","family":"Turcu\u0219","sequence":"additional","affiliation":[{"name":"Department of Botany, Faculty of Medicine, \u201cVasile Goldi\u015f\u201d Western University of Arad, 310414 Arad, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra C.","family":"Sevastre-Berghian","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Medicine, \u201cIuliu Ha\u0163ieganu\u201d University of Medicine and Pharmacy, 400006 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8695-6123","authenticated-orcid":false,"given":"Daniela","family":"Benedec","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy, \u201cIuliu Ha\u0163ieganu\u201d University of Medicine and Pharmacy, 400010 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Hanganu","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy, \u201cIuliu Ha\u0163ieganu\u201d University of Medicine and Pharmacy, 400010 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,12,18]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (2019). 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