{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T00:40:00Z","timestamp":1760402400119,"version":"build-2065373602"},"reference-count":34,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2022,1,6]],"date-time":"2022-01-06T00:00:00Z","timestamp":1641427200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>A histological analysis was performed with the aim of elucidating the spontaneous regeneration process of the hairy root lines LRT 2.3 and LRT 6.4, derived from Lopezia racemosa leaf explants and genetically transformed with the Agrobacterium\u00a0rhizogenes strain ATCC15834\/pTDT. The analysis showed both lines regenerate via indirect somatic embryogenesis; LRT 6.4 also regenerated by direct organogenesis. The morphogenic characteristics of the regenerated plantlets from both lines showed the typical characteristics, described previously, including a higher number of axillary shoot formation, short internodes, and plagiotropic roots compared with wild-type seedlings. The regeneration process occurred without the addition of plant growth regulators and was linked to the sucrose concentration in the culture medium. Reducing the sucrose concentration from 3% to 2%, 1%, and 0.5% increased the regeneration rate in LRT 6.4; the effect was less pronounced in LRT 2.3. The cytotoxic activity of different organic extracts obtained from roots and shoots were evaluated in the cancer cell lines HeLa (cervical carcinoma), HCT-15 (colon adenocarcinoma), and OVCAR (ovary carcinoma). The hexane and dichloromethane extracts from roots of both lines showed cytotoxic activity against the HeLa cell line. Only the dichloromethane extract from the roots of PLRT 2.3 showed cytotoxic activity against the OVCAR cell line. None of the methanol extracts showed cytotoxic activity, nor the shoot extracts from any solvent.<\/jats:p>","DOI":"10.3390\/plants11020150","type":"journal-article","created":{"date-parts":[[2022,1,9]],"date-time":"2022-01-09T20:21:56Z","timestamp":1641759716000},"page":"150","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Spontaneous Regeneration of Plantlets Derived from Hairy Root Cultures of Lopezia racemosa and the Cytotoxic Activity of Their Organic Extracts"],"prefix":"10.3390","volume":"11","author":[{"given":"Norely","family":"Vargas-Morales","sequence":"first","affiliation":[{"name":"Centro de Investigaci\u00f3n en Biotecnolog\u00eda, Universidad Aut\u00f3noma del Estado de Morelos, Av. Universidad 1001 Col. Chamilpa C.P., Cuernavaca 62209, Morelos, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5128-0781","authenticated-orcid":false,"given":"Norma Elizabeth","family":"Moreno-Anz\u00farez","sequence":"additional","affiliation":[{"name":"Centro de Desarrollo de Productos Bi\u00f3ticos, Instituto Polit\u00e9cnico Nacional, Carretera Yautepec-Jojutla Km 6, Calle CEPROBI No. 8, Colonia San Isidro C.P., Yautepec 62731, Morelos, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janeth","family":"T\u00e9llez-Rom\u00e1n","sequence":"additional","affiliation":[{"name":"Facultad de Medicina, Universidad Aut\u00f3noma del Estado de Morelos, Iztatz\u00edhuatl esq. Le\u00f1eros s\/n Col. Los Volcanes C.P., Cuernavaca 62350, Morelos, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irene","family":"Perea-Arango","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Biotecnolog\u00eda, Universidad Aut\u00f3noma del Estado de Morelos, Av. Universidad 1001 Col. Chamilpa C.P., Cuernavaca 62209, Morelos, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0089-226X","authenticated-orcid":false,"given":"Susana","family":"Valencia-D\u00edaz","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Biotecnolog\u00eda, Universidad Aut\u00f3noma del Estado de Morelos, Av. Universidad 1001 Col. Chamilpa C.P., Cuernavaca 62209, Morelos, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alfonso","family":"Leija-Salas","sequence":"additional","affiliation":[{"name":"Centro de Ciencias Gen\u00f3micas, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Av. Universidad s\/n Col. Chamilpa C.P., Cuernavaca 62210, Morelos, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edgar R.","family":"D\u00edaz-Garc\u00eda","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica del Sur, Instituto Mexicano del Seguro Social, Argentina No. 1 Col. Centro C.P., Xochitepec 62790, Morelos, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pilar","family":"Nicasio-Torres","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica del Sur, Instituto Mexicano del Seguro Social, Argentina No. 1 Col. Centro C.P., Xochitepec 62790, Morelos, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Del Carmen","family":"Guti\u00e9rrez-Villafuerte","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Biotecnolog\u00eda, Universidad Aut\u00f3noma del Estado de Morelos, Av. Universidad 1001 Col. Chamilpa C.P., Cuernavaca 62209, Morelos, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaime","family":"Tortoriello-Garc\u00eda","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica del Sur, Instituto Mexicano del Seguro Social, Argentina No. 1 Col. Centro C.P., Xochitepec 62790, Morelos, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jes\u00fas","family":"Arellano-Garc\u00eda","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Biotecnolog\u00eda, Universidad Aut\u00f3noma del Estado de Morelos, Av. Universidad 1001 Col. Chamilpa C.P., Cuernavaca 62209, Morelos, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,1,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"237438","DOI":"10.1155\/2013\/237438","article-title":"Antimicrobial, antiparasitic, anti-inflammatory, and cytotoxic activities of Lopezia racemosa","volume":"2013","author":"Bach","year":"2013","journal-title":"Sci. World J."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"8679","DOI":"10.3390\/molecules19068679","article-title":"Production of the Anti-Inflammatory Compound 6-O-Palmitoyl-3-O-\u03b2-D-glucopyranosylcampesterol by Callus Cultures of Lopezia racemosa Cav. (Onagraceae)","volume":"19","author":"Salinas","year":"2014","journal-title":"Molecules"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Moreno-Anz\u00farez, N.E., Marquina, S., \u00c1lvarez, L., Zamilpa, A., Castillo-Espa\u00f1a, P., Perea-Arango, I., Nicasio, M.P., Herrera-Ruiz, M., D\u00edaz-Garc\u00eda, E.R., and Tortoriello-Garc\u00eda, J. (2017). Cytotoxic and anti-inflammatory campesterol derivative from genetically transformed hairy roots of Lopezia racemosa Cav. (Onagraceae). Molecules, 22.","DOI":"10.3390\/molecules22010118"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2837","DOI":"10.1007\/s11033-012-2299-z","article-title":"Review: Role of carbon sources for in vitro plant growth and development","volume":"40","author":"Yaseen","year":"2013","journal-title":"Mol. Biol. 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