{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T16:14:26Z","timestamp":1790093666915,"version":"4.0.1"},"reference-count":83,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2022,3,29]],"date-time":"2022-03-29T00:00:00Z","timestamp":1648512000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Researchers Supporting Project","award":["RSP-2021\/103"],"award-info":[{"award-number":["RSP-2021\/103"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Today, skin care products and cosmetic preparations containing natural ingredients are widely preferred by consumers. Therefore, many cosmetic brands are encouraged to offer more natural products to the market, such as plant extracts that can be used for their antiaging, antiwrinkle, and depigmentation properties and other cosmetic purposes. In the current study, the volatile constituents of the hexane-soluble fraction of a Stenocarpus sinuatus (family Proteaceae) leaf methanol extract (SSHF) were analyzed using GC\/MS analysis. Moreover, the antiaging activity of SSHF was evaluated through in vitro studies of anti-collagenase, anti-elastase, anti-tyrosinase, and anti-hyaluronidase activities. In addition, an in silico docking study was carried out to identify the interaction mechanisms of the major compounds in SSHF with the active sites of the target enzymes. Furthermore, an in silico toxicity study of the identified compounds in SSHF was performed. It was revealed that vitamin E (\u03b1-tocopherol) was the major constituent of SSHF, representing 52.59% of the extract, followed by \u03b3-sitosterol (8.65%), neophytadiene (8.19%), \u03b2-tocopherol (6.07%), and others. The in vitro studies showed a significant inhibition by SSHF of collagenase, elastase, tyrosinase, and hyaluronidase, with IC50 values of 60.03, 177.5, 67.5, and 38.8 \u00b5g\/mL, respectively, comparable to those of the positive controls epigallocatechin gallate (ECGC, for collagenase, elastase, hyaluronidase) and kojic acid (for tyrosinase). Additionally, the molecular docking study revealed good acceptable binding scores of the four major compounds, comparable to those of ECGC and kojic acid. Besides, the SSHF identified phytoconstituents showed no predicted potential toxicity nor skin toxicity, as determined in silico. In conclusion, the antiaging potential of SSHF may be attributed to its high content of vitamin E in addition to the synergetic effect of other volatile constituents. Thus, SSHF could be incorporated in pharmaceutical skin care products and cosmetics after further studies.<\/jats:p>","DOI":"10.3390\/plants11070918","type":"journal-article","created":{"date-parts":[[2022,3,29]],"date-time":"2022-03-29T21:41:30Z","timestamp":1648590090000},"page":"918","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":96,"title":["GC\/MS Profiling, Anti-Collagenase, Anti-Elastase, Anti-Tyrosinase and Anti-Hyaluronidase Activities of a Stenocarpus sinuatus Leaves Extract"],"prefix":"10.3390","volume":"11","author":[{"given":"Mai M.","family":"Younis","sequence":"first","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams University, Abbassia, Cairo 11566, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2382-8241","authenticated-orcid":false,"given":"Iriny M.","family":"Ayoub","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams University, Abbassia, Cairo 11566, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1602-2725","authenticated-orcid":false,"given":"Nada M.","family":"Mostafa","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams University, Abbassia, Cairo 11566, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6795-4816","authenticated-orcid":false,"given":"Mahmoud A.","family":"El Hassab","sequence":"additional","affiliation":[{"name":"Department of Medicinal Chemistry, Faculty of Pharmacy, King Salman International University (KSIU), South Sinai 46612, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6996-4017","authenticated-orcid":false,"given":"Wagdy M.","family":"Eldehna","sequence":"additional","affiliation":[{"name":"School of Biotechnology, Badr University in Cairo, Badr City, Cairo 11829, Egypt"},{"name":"Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Kafrelsheikh University, Kafrelsheikh 33516, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara T.","family":"Al-Rashood","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0972-0560","authenticated-orcid":false,"given":"Omayma A.","family":"Eldahshan","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams University, Abbassia, Cairo 11566, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,3,29]]},"reference":[{"key":"ref_1","first-page":"14","article-title":"The glycative stress and skin aging, and its countermeasure","volume":"45","author":"Yagi","year":"2017","journal-title":"Fragr. J."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"308","DOI":"10.4161\/derm.22804","article-title":"Skin anti-aging strategies","volume":"4","author":"Ganceviciene","year":"2012","journal-title":"Derm.-Endocrinol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1007\/s10522-017-9715-7","article-title":"Plant extracts and natural compounds used against UVB-induced photoaging","volume":"18","author":"Cavinato","year":"2017","journal-title":"Biogerontology"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"436","DOI":"10.15414\/jmbfs.2020.10.3.436-440","article-title":"Determination of antioxidant, anti-aging and cytotoxicity activity of the essential oils from Cinnamomum zeylanicum","volume":"10","author":"Banglao","year":"2020","journal-title":"J. Microbiol. Biotechnol. Food Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.chroma.2017.11.003","article-title":"Use of chromatographic and electrophoretic tools for assaying elastase, collagenase, hyaluronidase, and tyrosinase activity","volume":"1529","author":"Fayad","year":"2017","journal-title":"J. Chromatogr. A"},{"key":"ref_6","first-page":"329","article-title":"Assessment of cosmeceutical potentials of selected mushroom fruitbody extracts through evaluation of antioxidant, anti-hyaluronidase and anti-tyrosinase activity","volume":"3","author":"Jamaluddin","year":"2020","journal-title":"J"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Mostafa, N.M., Mostafa, A.M., Ashour, M.L., and Elhady, S.S. (2021). Neuroprotective effects of black pepper cold-pressed oil on scopolamine-induced oxidative stress and memory impairment in rats. Antioxidants, 10.","DOI":"10.3390\/antiox10121993"},{"key":"ref_8","first-page":"1182","article-title":"A new antidiabetic and anti-inflammatory biflavonoid from Schinus polygama (Cav.) Cabrera leaves","volume":"36","author":"Mostafa","year":"2020","journal-title":"Nat. Prod. Res."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Abdallah, S.H., Mostafa, N.M., Mohamed, M.A., Nada, A.S., and Singab, A.N.B. (2021). UPLC-ESI-MS\/MS profiling and hepatoprotective activities of Stevia leaves extract, butanol fraction and stevioside against radiation-induced toxicity in rats. Nat. Prod. Res., 1\u20137.","DOI":"10.1080\/14786419.2021.2015594"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"6267","DOI":"10.1039\/C9FO01532A","article-title":"Metabolic profiling of a polyphenolic-rich fraction of Coccinia grandis leaves using LC-ESI-MS\/MS and in vivo validation of its antimicrobial and wound healing activities","volume":"10","author":"Mostafa","year":"2019","journal-title":"Food Funct."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"e1700528","DOI":"10.1002\/cbdv.201700528","article-title":"New methoxyflavone from Casimiroa sapota and the biological activities of its leaves extract against lead acetate induced hepatotoxicity in rats","volume":"15","author":"Mostafa","year":"2018","journal-title":"Chem. Biodivers."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"e1600156","DOI":"10.1002\/cbdv.201600156","article-title":"Chemical composition and biological activities of the essential oil from leaves and flowers of Pulicaria incisa sub. candolleana (Family Asteraceae)","volume":"14","author":"Shahat","year":"2017","journal-title":"Chem. Biodivers."},{"key":"ref_13","first-page":"1934578X1400900731","article-title":"Profile of volatile components of hydrodistilled and extracted leaves of Jacaranda acutifolia and their antimicrobial activity against foodborne pathogens","volume":"9","author":"Singab","year":"2014","journal-title":"Nat. Prod. Commun."},{"key":"ref_14","first-page":"5256781","article-title":"Schinus terebinthifolius essential oil attenuates scopolamine-induced memory deficits via cholinergic modulation and antioxidant properties in a zebrafish model","volume":"2019","author":"Mostafa","year":"2019","journal-title":"Evid.-Based Complement. Altern. Med."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"218","DOI":"10.3389\/fphar.2020.00218","article-title":"Hepatoprotective effect of cranberry nutraceutical extract in non-alcoholic fatty liver model in rats: Impact on insulin resistance and Nrf-2 expression","volume":"11","author":"Faheem","year":"2020","journal-title":"Front. Pharmacol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1029","DOI":"10.1080\/14786419.2019.1613396","article-title":"Renoprotective effect of tectorigenin glycosides isolated from Iris spuria L. (Zeal) against hyperoxaluria and hyperglycemia in NRK-49Fcells","volume":"35","author":"Divya","year":"2021","journal-title":"Nat. Prod. Res."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Ayoub, I.M., George, M.Y., Menze, E.T., Mahmoud, M., Botros, M., Essam, M., Ashmawy, I., Shendi, P., Hany, A., and Galal, M. (2022). Insights into the neuroprotective effects of Salvia officinalis L. and Salvia microphylla Kunth in the memory impairment rat model. Food Funct.","DOI":"10.1039\/D1FO02988F"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Elkady, W.M., Ayoub, I.M., Abdel-Mottaleb, Y., ElShafie, M.F., and Wink, M. (2020). Euryops pectinatus L. Flower extract inhibits P-glycoprotein and reverses multi-drug resistance in cancer cells: A mechanistic study. Molecules, 25.","DOI":"10.3390\/molecules25030647"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Brinza, I., Ayoub, I.M., Eldahshan, O.A., and Hritcu, L. (2021). Baicalein 5,6-dimethyl ether prevents memory deficits in the scopolamine zebrafish model by regulating cholinergic and antioxidant systems. Plants, 10.","DOI":"10.3390\/plants10061245"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"16868","DOI":"10.1038\/s41598-021-96377-6","article-title":"Green synthesis of magnesium nanoparticles mediated from Rosa floribunda charisma extract and its antioxidant, antiaging and antibiofilm activities","volume":"11","author":"Younis","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"160","DOI":"10.3923\/rjmp.2015.160.169","article-title":"Antifungal and antibacterial activity of crude stem bark extracts\u2019 of Bersama abysinicca Verdc. and Faurea saligna Harr","volume":"9","author":"Amuka","year":"2015","journal-title":"Res. J. Med. Plant"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1241","DOI":"10.1021\/acs.jnatprod.7b01038","article-title":"Arbutin derivatives isolated from ancient Proteaceae: Potential phytochemical markers present in Bellendena, Cenarrhenes, and Persoonia Genera","volume":"81","author":"Deans","year":"2018","journal-title":"J. Nat. Prod."},{"key":"ref_23","first-page":"1934578X1601100322","article-title":"Cytotoxic activity of lomatiol and 7-(3\u2032-Hydroxymethyl-3\u2032-methylallyloxy)coumarin","volume":"11","author":"Fiorito","year":"2016","journal-title":"Nat. Prod. Commun."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"628","DOI":"10.1007\/s11094-019-02051-7","article-title":"Evaluation of the antioxidant, hepatoprotective, and anti-inflammatory activities of bisresorcinol isolated from the trunk of Heliciopsis Terminalis","volume":"53","author":"Giang","year":"2019","journal-title":"Pharm. Chem. J."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1353129","DOI":"10.1155\/2018\/1353129","article-title":"Flavonoids in Ecuadorian Oreocallis grandiflora (Lam.) R. Br.: Perspectives of use of this species as a food supplement","volume":"2018","author":"Vinueza","year":"2018","journal-title":"Evid.-Based Complement. Altern. Med."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1812","DOI":"10.1002\/hlca.201100089","article-title":"Bisresorcinol derivatives from Grevillea glauca","volume":"94","author":"Wang","year":"2011","journal-title":"Helv. Chim. Acta"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"736","DOI":"10.1016\/j.tetlet.2017.01.021","article-title":"Tropane alkaloids from the Australian plant Triunia montana (Proteaceae)","volume":"58","author":"Yang","year":"2017","journal-title":"Tetrahedron Lett."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"32","DOI":"10.3390\/medicines1010032","article-title":"Antileishmanial potential of tropical rainforest plant extracts","volume":"1","author":"Monzote","year":"2014","journal-title":"Medicines"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.intimp.2018.11.052","article-title":"Antidepressant effect of helicid in chronic unpredictable mild stress model in rats","volume":"67","author":"Tong","year":"2019","journal-title":"Int. Immunopharmacol."},{"key":"ref_30","first-page":"1089","article-title":"Phytochemical constituents and anti-tyrosinase activity of Macadamia integrifolia leaves extract","volume":"36","author":"Abubaker","year":"2020","journal-title":"Nat. Prod. Res."},{"key":"ref_31","first-page":"1442","article-title":"Study of Nutritional contents of Macadamia integrifolia Maiden & Betche leaves, kernel and pericarp cultivated in Egypt","volume":"9","author":"Abubaker","year":"2017","journal-title":"Int. J. Pharmacogn. Phytochem. Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1103","DOI":"10.1016\/j.foodchem.2010.01.057","article-title":"Functional lipid characteristics, oxidative stability, and antioxidant activity of macadamia nut (Macadamia integrifolia) cultivars","volume":"121","author":"Wall","year":"2010","journal-title":"Food Chem."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"824","DOI":"10.1016\/j.jep.2013.02.010","article-title":"Tyrosinase modulation by five Rwandese herbal medicines traditionally used for skin treatment","volume":"146","author":"Kamagaju","year":"2013","journal-title":"J. Ethnopharmacol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"9331","DOI":"10.3390\/molecules16119331","article-title":"Chemical constituents and biological studies of the leaves of Grevillea robusta","volume":"16","author":"Chuang","year":"2011","journal-title":"Molecules"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"276","DOI":"10.22159\/ajpcr.2018.v11i2.20910","article-title":"Oreocallis grandiflora photoprotective effect against ultraviolet B radiation-induced cell death","volume":"11","author":"Vinueza","year":"2018","journal-title":"Asian J. Pharm. Clin. Res."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1121","DOI":"10.1071\/CH9731121","article-title":"Chemical studies of the Proteaceae. VI. Two naphthoquinones from Stenocarpus salignus","volume":"26","author":"Mock","year":"1973","journal-title":"Aust. J. Chem."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"165","DOI":"10.3897\/jhr.81.59365","article-title":"Missing for almost 100 years: The rare and potentially threatened bee, Pharohylaeus lactiferus (Hymenoptera, Colletidae)","volume":"81","author":"Dorey","year":"2021","journal-title":"J. Hymenopt. Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"116802","DOI":"10.1016\/j.molliq.2021.116802","article-title":"Encapsulation of \u03b1-tocopherol in large-ring cyclodextrin containing 26 \u03b1-D-glucopyranose units: A molecular dynamics study","volume":"339","author":"Kerdpol","year":"2021","journal-title":"J. Mol. Liq."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Bruno, R.S., Mah, E., and Vitamin, E. (2014). Reference Module in Biomedical Sciences, Elsevier.","DOI":"10.1016\/B978-0-12-801238-3.00231-2"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"3101","DOI":"10.1021\/jf000891u","article-title":"Alpha-tocopherol content in 62 edible tropical plants","volume":"49","author":"Ching","year":"2001","journal-title":"J. Agric. Food Chem."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1021","DOI":"10.1007\/s00217-018-3206-1","article-title":"Chemical evaluation and thermal behavior of Chilean hazelnut oil (Gevuina avellana Mol) a comparative study with extra virgin olive oil","volume":"245","author":"Vergara","year":"2019","journal-title":"Eur. Food Res. Technol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"687","DOI":"10.17660\/ActaHortic.2009.845.108","article-title":"Fractions of vitamin E (tocotrienols and tocopherols) in nut oil of Gevuina avellana Mol","volume":"845","author":"Medel","year":"2009","journal-title":"Acta Hortic."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1111\/jocd.13305","article-title":"Anti-aging and brightening effects of a topical treatment containing vitamin C, vitamin E, and raspberry leaf cell culture extract: A split-face, randomized controlled trial","volume":"19","author":"Rattanawiwatpong","year":"2020","journal-title":"J. Cosmet. Dermatol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"340107","DOI":"10.1155\/2013\/340107","article-title":"Activation of MITF by argan oil leads to the inhibition of the tyrosinase and dopachrome tautomerase expressions in B16 murine melanoma cells","volume":"2013","author":"Villareal","year":"2013","journal-title":"Evid.-Based Complement. Altern. Med."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1016\/S0891-5849(99)00007-6","article-title":"Age-dependent increase of collagenase expression can be reduced by \u03b1-tocopherol via protein kinase C inhibition","volume":"27","author":"Ricciarelli","year":"1999","journal-title":"Free. Radic. Biol. Med."},{"key":"ref_46","first-page":"115","article-title":"Antioxidative activities and tyrosinase inhibitory effects of Korean medicinal plants","volume":"50","author":"Heo","year":"2007","journal-title":"J. Appl. Biol. Chem."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"807","DOI":"10.1016\/j.carbpol.2010.07.002","article-title":"Release characteristics of vitamin E incorporated chitosan microspheres and in vitro\u2013in vivo evaluation for topical application","volume":"84","author":"Yenilmez","year":"2011","journal-title":"Carbohydr. Polym."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Polj\u0161ak, N., and Ko\u010devar Glava\u010d, N. (2021). Tilia sp. Seed Oil\u2014Composition, antioxidant activity and potential use. Appl. Sci., 11.","DOI":"10.3390\/app11114932"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1111\/j.1467-2494.2005.00259_1.x","article-title":"Cutaneous restructuration by apple seed phytosterols: From DNA chip analysis to morphological alterations","volume":"27","author":"Doering","year":"2005","journal-title":"Int. J. Cosmet. Sci."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"5282","DOI":"10.1080\/14786419.2020.1753048","article-title":"Two clerodane diterpenes isolated from Polyalthia longifolia leaves: Comparative structural features, anti-histaminic and anti-Helicobacter pylori activities","volume":"35","author":"Edmond","year":"2021","journal-title":"Nat. Prod. Res."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Mostafa, N.M., Edmond, M.P., El-Shazly, M., Fahmy, H.A., Sherif, N.H., and Singab, A.N.B. (2021). Phytoconstituents and renoprotective effect of Polyalthia longifolia leaves extract on radiation-induced nephritis in rats via TGF-\u03b2\/smad pathway. Nat. Prod. Res., 1\u20136.","DOI":"10.1080\/14786419.2021.1961252"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1186\/s41702-018-0025-8","article-title":"Inhibitory effect of phytol on cellular senescence","volume":"2","author":"Jeong","year":"2018","journal-title":"Biomed. Dermatol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.fct.2018.08.032","article-title":"Phytol: A review of biomedical activities","volume":"121","author":"Islam","year":"2018","journal-title":"Food Chem. Toxicol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1177932219896538","DOI":"10.1177\/1177932219896538","article-title":"Interaction of selected terpenoids from Dalbergia sissoo with catalytic domain of matrix metalloproteinase-1: An in silico assessment of their anti-wrinkling potential","volume":"13","author":"Yasmeen","year":"2019","journal-title":"Bioinform. Biol. Insights"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"395","DOI":"10.5530\/pj.2016.4.14","article-title":"GC- MS Analysis of Mussaenda roxburghii Hk.f.: A folk food plant used among tribes of Arunachal Pradesh, India","volume":"8","author":"Temin","year":"2016","journal-title":"Pharmacogn. J."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1002\/ffj.3661","article-title":"Chemical composition and biological activities of essential oils from Calendula officinalis L. flowers and leaves","volume":"36","author":"Ak","year":"2021","journal-title":"Flavour Fragr. J."},{"key":"ref_57","first-page":"2748","article-title":"Gas chromatography and mass spectrometry analysis of bioactive constituents of Maytenus heyneana (Roth) Roju & Babu (Celestraceae)","volume":"8","author":"Mahalakashmi","year":"2019","journal-title":"J. Pharmacogn. Phytochem."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"2093","DOI":"10.1080\/14786419.2015.1114938","article-title":"Cytotoxic activity and molecular docking of a novel biflavonoid isolated from Jacaranda acutifolia (Bignoniaceae)","volume":"30","author":"Mostafa","year":"2016","journal-title":"Nat. Prod. Res."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"2715","DOI":"10.1080\/14786419.2019.1585846","article-title":"Pulchranin A: First report of isolation from an endophytic fungus and its inhibitory activity on cyclin dependent kinases","volume":"34","author":"Moussa","year":"2020","journal-title":"Nat. Prod. Res."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"903","DOI":"10.1080\/0972060X.2019.1667877","article-title":"GC\/MS analysis and molecular profiling of lemon volatile oil against breast cancer","volume":"22","author":"Ashmawy","year":"2019","journal-title":"J. Essent. Oil Bear. Plants"},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Elhawary, E.A., Mostafa, N.M., Labib, R.M., and Singab, A.N. (2021). Metabolomic profiles of essential oils from selected rosa varieties and their antimicrobial activities. Plants, 10.","DOI":"10.3390\/plants10081721"},{"key":"ref_62","first-page":"5369","article-title":"Chemical composition, antimicrobial and cytotoxic activities of essential oils from Schinus polygamus (Cav.) cabrera leaf and bark grown in Egypt","volume":"35","author":"Mostafa","year":"2020","journal-title":"Nat. Prod. Res."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"4133","DOI":"10.1080\/14786419.2020.1736064","article-title":"Chemical composition, cytotoxicity and molecular profiling of Cordia africana Lam. on human breast cancer cell line","volume":"35","author":"Ashmawy","year":"2020","journal-title":"Nat. Prod. Res."},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Labib, R.M., Ayoub, I.M., Michel, H.E., Mehanny, M., Kamil, V., Hany, M., Magdy, M., Moataz, A., Maged, B., and Mohamed, A. (2019). Appraisal on the wound healing potential of Melaleuca alternifolia and Rosmarinus officinalis L. essential oil-loaded chitosan topical preparations. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0219561"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"820","DOI":"10.1002\/pca.3028","article-title":"Phytochemical discrimination of Pinus species based on GC\u2013MS and ATR-IR analyses and their impact on Helicobacter pylori","volume":"32","author":"Gad","year":"2021","journal-title":"Phytochem. Anal."},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Gad, H.A., Ayoub, I.M., and Wink, M. (2019). Phytochemical profiling and seasonal variation of essential oils of three Callistemon species cultivated in Egypt. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0219571"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1080\/0972060X.2010.10643827","article-title":"Volatile constituents of leaves of Ficus carica Linn. grown in Egypt","volume":"13","author":"Ayoub","year":"2010","journal-title":"J. Essent. Oil Bear. Plants"},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Ayoub, I.M., Korinek, M., El-Shazly, M., Wetterauer, B., El-Beshbishy, H.A., Hwang, T.-L., Chen, B.-H., Chang, F.-R., Wink, M., and Singab, A.N.B. (2021). Anti-allergic, anti-inflammatory, and anti-hyperglycemic activity of Chasmanthe aethiopica leaf extract and its profiling using LC\/MS and GLC\/MS. Plants, 10.","DOI":"10.3390\/plants10061118"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.sajb.2021.06.013","article-title":"The impact of seasonal variation on the volatile profile of leaves and stems of Brownea grandiceps (Jacq.) with evaluation of their anti-mycobacterial and anti-inflammatory activities","volume":"142","author":"Korany","year":"2021","journal-title":"S. Afr. J. Bot."},{"key":"ref_70","doi-asserted-by":"crossref","unstructured":"Thabet, A.A., Ayoub, I.M., Youssef, F.S., Al Sayed, E., and Singab, A.N.B. (2021). Essential oils from the leaves and flowers of Leucophyllum frutescens (Scrophulariaceae): Phytochemical analysis and inhibitory effects against elastase and collagenase in vitro. Nat. Prod. Res., 1\u20135.","DOI":"10.1080\/14786419.2021.2000981"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"1654","DOI":"10.1080\/13880209.2017.1314512","article-title":"Anti-inflammatory and gastroprotective potential of leaf essential oil of Cinnamomum glanduliferum in ethanol-induced rat experimental gastritis","volume":"55","author":"Azab","year":"2017","journal-title":"Pharm. Biol."},{"key":"ref_72","doi-asserted-by":"crossref","unstructured":"Brinza, I., Abd-Alkhalek, A.M., El-Raey, M.A., Boiangiu, R.S., Eldahshan, O.A., and Hritcu, L. (2020). Ameliorative effects of rhoifolin in scopolamine-induced amnesic zebrafish (Danio rerio) model. Antioxidants, 9.","DOI":"10.3390\/antiox9070580"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1472-6882-9-27","article-title":"Anti-collagenase, anti-elastase and anti-oxidant activities of extracts from 21 plants","volume":"9","author":"Thring","year":"2009","journal-title":"BMC Complementary Altern. Med."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1016\/j.bbrc.2004.05.163","article-title":"Inhibition effects of (+)-catechin-aldehyde polycondensates on proteinases causing proteolytic degradation of extracellular matrix","volume":"320","author":"Kim","year":"2004","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"138","DOI":"10.3923\/jbs.2010.138.144","article-title":"Potency of Indonesian medicinal plants as tyrosinase inhibitor and antioxidant agent","volume":"10","author":"Batubara","year":"2010","journal-title":"J. Biol. Sci."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"959","DOI":"10.1016\/S0021-9258(18)65959-9","article-title":"A modified colorimetric method for the estimation of N-acetylamino sugars","volume":"217","author":"Reissig","year":"1955","journal-title":"J. Biol. Chem."},{"key":"ref_77","first-page":"57","article-title":"Python: A programming language for software integration and development","volume":"17","author":"Sanner","year":"1999","journal-title":"J. Mol. Graph. Model."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1002\/jcc.21334","article-title":"Improving the speed and accuracy of docking with a new scoring function, efficient optimization, and multithreading Trott, Oleg; Olson, Arthur J","volume":"31","author":"Vina","year":"2010","journal-title":"J. Comput. Chem."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"1668","DOI":"10.1002\/jcc.20290","article-title":"The Amber biomolecular simulation programs","volume":"26","author":"Case","year":"2005","journal-title":"J. Comput. Chem."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"1555","DOI":"10.2174\/156802608786786624","article-title":"Medicinal chemistry and the molecular operating environment (MOE): Application of QSAR and molecular docking to drug discovery","volume":"8","author":"Vilar","year":"2008","journal-title":"Curr. Top. Med. Chem."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1080\/14756366.2020.1868450","article-title":"Development of novel isatin\u2013nicotinohydrazide hybrids with potent activity against susceptible\/resistant Mycobacterium tuberculosis and bronchitis causing\u2013bacteria","volume":"36","author":"Elsayed","year":"2021","journal-title":"J. Enzym. Inhib. Med. Chem."},{"key":"ref_82","doi-asserted-by":"crossref","unstructured":"Banerjee, P., Dehnbostel, F.O., and Preissner, R. (2018). Prediction is a balancing act: Importance of sampling methods to balance sensitivity and specificity of predictive models based on imbalanced chemical data sets. Front. Chem., 362.","DOI":"10.3389\/fchem.2018.00362"},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"1013","DOI":"10.1021\/acs.jcim.7b00194","article-title":"Pred-skin: A fast and reliable web application to assess skin sensitization effect of chemicals","volume":"57","author":"Braga","year":"2017","journal-title":"J. Chem. Inf. Model."}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/11\/7\/918\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T22:46:01Z","timestamp":1760136361000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/11\/7\/918"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,3,29]]},"references-count":83,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2022,4]]}},"alternative-id":["plants11070918"],"URL":"https:\/\/doi.org\/10.3390\/plants11070918","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,3,29]]}}}