{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T05:02:34Z","timestamp":1787029354209,"version":"build-2736575974"},"reference-count":62,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2022,7,18]],"date-time":"2022-07-18T00:00:00Z","timestamp":1658102400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"The Deanship of Scientific Research at Jouf University","award":["DSR-2021-03-0218"],"award-info":[{"award-number":["DSR-2021-03-0218"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Origanum majoranum L. is a Lamiaceae medicinal plant with culinary and ethnomedical applications. Its biological and phytochemical profiles have been extensively researched. Accordingly, this study aimed to investigate the chemical composition and the antibacterial and antioxidant properties of O. majoranum high features, as well as to search for techniques for activity optimization. A metabolomics study of the crude extract of O. majoranum using liquid chromatography-high-resolution electrospray ionization mass spectrometry (LC \u00b1 HR \u00b1 ESI \u00b1 MS) was conducted. Five fractions (petroleum ether, dichloromethane, ethyl acetate, n-butanol, and aqueous) were derived from the total extract of the aerial parts. Different chromatographic methods and NMR analysis were utilized to purify and identify the isolated phenolics (high features). Moreover, the antimicrobial, antibiofilm, and antioxidant activity of phenolics were performed. Results showed that metabolomic profiling of the crude extract of O. majoranum aerial parts revealed the presence of a variety of phytochemicals, predominantly phenolics, resulting in the isolation and identification of seven high-feature compounds comprising two phenolic acids, rosmarinic and caffeic acids, one phenolic diterpene, 7-methoxyepirosmanol, in addition to four flavonoids, quercetin, hesperitin, hesperidin, and luteolin. On the other hand, 7-methoxyepirosmanol (OM1) displayed the most antimicrobial and antioxidant potential. Such a phenolic principal activity improvement seems to be established after loading on gold nanoparticles.<\/jats:p>","DOI":"10.3390\/plants11141871","type":"journal-article","created":{"date-parts":[[2022,7,19]],"date-time":"2022-07-19T00:19:21Z","timestamp":1658189961000},"page":"1871","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["LC\/MS Profiling and Gold Nanoparticle Formulation of Major Metabolites from Origanum majorana as Antibacterial and Antioxidant Potentialities"],"prefix":"10.3390","volume":"11","author":[{"given":"Ahmed H.","family":"El-Ghorab","sequence":"first","affiliation":[{"name":"Department of Chemistry, College of Science, Jouf University, Sakaka 72341, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5889-034X","authenticated-orcid":false,"given":"Fathy A.","family":"Behery","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt"},{"name":"Department of Pharmacy, College of Pharmacy, Riyadh Elm University, Riyadh 11681, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9035-5638","authenticated-orcid":false,"given":"Mohamed A.","family":"Abdelgawad","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry, College of Pharmacy, Jouf University, Sakaka 72341, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ibrahim Hotan","family":"Alsohaimi","sequence":"additional","affiliation":[{"name":"Department of Chemistry, College of Science, Jouf University, Sakaka 72341, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arafa","family":"Musa","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, College of Pharmacy, Jouf University, Sakaka 72341, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8841-9786","authenticated-orcid":false,"given":"Ehab M.","family":"Mostafa","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, College of Pharmacy, Jouf University, Sakaka 72341, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5115-3179","authenticated-orcid":false,"given":"Hamud A.","family":"Altaleb","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Science, Islamic University of Madinah, Madinah 41477, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ibrahim O.","family":"Althobaiti","sequence":"additional","affiliation":[{"name":"Department of Chemistry, College of Science and Arts, Jouf University, Sakaka 72341, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed","family":"Hamza","sequence":"additional","affiliation":[{"name":"Department of Biology, College of Science, Jouf University, Sakaka 72341, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4083-6024","authenticated-orcid":false,"given":"Mohammed H.","family":"Elkomy","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics, College of Pharmacy, Jouf University, Sakaka 72341, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9997-9284","authenticated-orcid":false,"given":"Ahmed A.","family":"Hamed","sequence":"additional","affiliation":[{"name":"National Research Centre, Microbial Chemistry Department, 33 El-Buhouth Street, Dokki, Giza 12622, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1442-183X","authenticated-orcid":false,"given":"Ahmed M.","family":"Sayed","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy, Nahda University, Beni-Suef 62513, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0174-4434","authenticated-orcid":false,"given":"Hossam M.","family":"Hassan","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62513, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahmoud A.","family":"Aboseada","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy, Nahda University, Beni-Suef 62513, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,18]]},"reference":[{"key":"ref_1","first-page":"9785932","article-title":"Renoprotective effects of Origanum majorana methanolic L and carvacrol on ischemia\/reperfusion-induced kidney injury in male rats. Evidence-Based Complement","volume":"2020","author":"Gheitasi","year":"2020","journal-title":"Altern. Med."},{"key":"ref_2","first-page":"817","article-title":"Cytotoxic effect of synergism relationship of oil extract from Origanum majorana L. and silicon nano particles on MCF-7","volume":"20","author":"Jssim","year":"2020","journal-title":"Plant Arch."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"8261","DOI":"10.1021\/jf049113i","article-title":"Essential oils of Satureja, Origanum, and Thymus species: Chemical composition and antibacterial activities against foodborne pathogens","volume":"52","author":"Chorianopoulos","year":"2004","journal-title":"J. Agric. Food Chem."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1015","DOI":"10.1007\/s10600-012-0133-4","article-title":"Essential oil composition of Origanum majorana and Origanum vulgare ssp. hirtum growing in India. Chem","volume":"47","author":"Raina","year":"2012","journal-title":"Nat. Compd."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Kosakowska, O., and Czupa, W. (2018). Morphological and chemical variability of common oregano (Origanum vulgare L. subsp vulgare) occurring in eastern Poland. Herba Pol., 64.","DOI":"10.2478\/hepo-2018-0001"},{"key":"ref_6","unstructured":"Danin, A., and K\u00fcnne, I. (1996). Origanum jordanicum (Labiatae), a new species from Jordan, and notes on the other species of O. sect. Campanulaticalyx. Willdenowia, 601\u2013611."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1016\/j.jep.2007.11.045","article-title":"Medicinal plants in the Mediterranean area: Synthesis of the results of the project Rubia","volume":"116","author":"Pieroni","year":"2008","journal-title":"J. Ethnopharmacol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2297","DOI":"10.1021\/jf0112872","article-title":"Recovery of pigments from Origanum majorana L. by extraction with supercritical carbon dioxide","volume":"50","author":"Vagi","year":"2002","journal-title":"J. Agric. Food Chem."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1002\/ffj.1077","article-title":"Essential oil compounds in a historical sample of marjoram (Origanum majorana L., Lamiaceae)","volume":"17","author":"Novak","year":"2002","journal-title":"Flavour Fragr. J."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.vph.2007.11.003","article-title":"Comparative effects of Artemisia dracunculus, Satureja hortensis and Origanum majorana on inhibition of blood platelet adhesion, aggregation and secretion","volume":"48","author":"Yazdanparast","year":"2008","journal-title":"Vascul. Pharmacol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"8162","DOI":"10.1021\/jf0510079","article-title":"Antibacterial and antioxidant activities of essential oils isolated from Thymbra capitata L. (Cav.) and Origanum vulgare L.","volume":"53","author":"Faleiro","year":"2005","journal-title":"J. Agric. Food Chem."},{"key":"ref_12","unstructured":"Bremness, L., and King, D. (1988). The Complete Book of Herbs, Viking Studio Books."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1039\/c1fo10026b","article-title":"Inhibition of advanced glycation endproduct formation by foodstuffs","volume":"2","author":"Wu","year":"2011","journal-title":"Food Funct."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"102","DOI":"10.3923\/ajdd.2014.102.112","article-title":"Antigout and antioxidant activity of stem and root of Origanum majorana Linn","volume":"4","author":"Vasudeva","year":"2014","journal-title":"Am. J. Drug Discov. Dev."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"611","DOI":"10.3923\/rjbsci.2011.611.614","article-title":"Comparative study of sedative, pre-anesthetic and anti-anxiety effect of Origanum majorana extract with diazepam on rats","volume":"6","author":"Rezaie","year":"2011","journal-title":"Res. J. Biol. Sci."},{"key":"ref_16","first-page":"64","article-title":"Anticonvulsant effect of Origanum majorana L.","volume":"2007","author":"Deshmane","year":"2007","journal-title":"Pharmacologyonline"},{"key":"ref_17","first-page":"5479","article-title":"Effect of marjoram extract treatment on the cytological and biochemical changes induced by cyclophosphamide in mice","volume":"5","year":"2011","journal-title":"J. Med. Plants Res."},{"key":"ref_18","first-page":"1428","article-title":"Pharmacognostical and quality control parameters of Origanum majorana Linn. Stem root","volume":"3","author":"Singla","year":"2014","journal-title":"World J. Pharm. Pharm. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/S1016-8478(23)17016-6","article-title":"Inhibitory Effect of Ursolic Acid Purified from Origanum majorana L. on the Acetylcholinesterase","volume":"11","author":"Chung","year":"2001","journal-title":"Mol. Cells"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1016\/j.indcrop.2009.07.010","article-title":"Effect of growth stage on the content and composition of the essential oil and phenolic fraction of sweet marjoram (Origanum majorana L.)","volume":"30","author":"Sellami","year":"2009","journal-title":"Ind. Crops Prod."},{"key":"ref_21","unstructured":"Khan, I.A., and Abourashed, E.A. (2011). Leung\u2019s Encyclopedia of Common Natural Ingredients: Used in Food, Drugs and Cosmetics, John Wiley & Sons."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1016\/S0305-1978(99)00007-1","article-title":"Comparative studies of the rosmarinic and caffeic acid contents of Lamiaceae species","volume":"27","author":"Blunden","year":"1999","journal-title":"Biochem. Syst. Ecol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1038\/sj.jim.7000057","article-title":"LC-NMR: A new tool to expedite the dereplication and identification of natural products","volume":"25","author":"Bobzin","year":"2000","journal-title":"J. Ind. Microbiol. Biotechnol."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Tawfike, A.F., Viegelmann, C., and Edrada-Ebel, R. (2013). Metabolomics and dereplication strategies in natural products. Metabolomics Tools for Natural Product Discovery, Humana Press.","DOI":"10.1007\/978-1-62703-577-4_17"},{"key":"ref_25","unstructured":"Blunt, J. (2012). MarinLit, University of Canterbury."},{"key":"ref_26","unstructured":"Laatsch, H. (2012). Antibase Version 4.0\u2014The Natural Compound Identifier, Wiley-VCH Verlag GmbH & Co. KGaA."},{"key":"ref_27","first-page":"155","article-title":"Combining genomics, metabolome analysis, and biochemical modelling to understand metabolic networks","volume":"2","author":"Fiehn","year":"2001","journal-title":"Int. J. Genom."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1007\/s10295-005-0231-4","article-title":"Microbial metabolomics: Replacing trial-and-error by the unbiased selection and ranking of targets","volume":"32","author":"Jellema","year":"2005","journal-title":"J. Ind. Microbiol. Biotechnol."},{"key":"ref_29","first-page":"145","article-title":"Antimicrobial activity of methanol extract of Origanum majorana L. (Sweet marjoram)","volume":"28","author":"Leeja","year":"2007","journal-title":"J. Environ. Biol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"822","DOI":"10.1002\/jsfa.7155","article-title":"Isolation and identification of chemical constituents from Origanum majorana and investigation of antiproliferative and antioxidant activities","volume":"96","author":"Erenler","year":"2016","journal-title":"J. Sci. Food Agric."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1021\/jf048777p","article-title":"Phenolic and triterpenoid antioxidants from Origanum majorana L. herb and extracts obtained with different solvents","volume":"53","author":"Vagi","year":"2005","journal-title":"J. Agric. Food Chem."},{"key":"ref_32","first-page":"925","article-title":"Optical solitons to the fractional perturbed NLSE in nano-fibers","volume":"13","author":"Sulaiman","year":"2020","journal-title":"Discret. Contin. Dyn. Syst."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1177\/0148607111434963","article-title":"Oxidative stress","volume":"36","author":"Preiser","year":"2012","journal-title":"J. Parenter. Enter. Nutr."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/j.abb.2015.11.014","article-title":"Dietary polyphenols: Antioxidants or not?","volume":"595","author":"Croft","year":"2016","journal-title":"Arch. Biochem. Biophys."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.jpha.2015.11.005","article-title":"Methods for in vitro evaluating antimicrobial activity: A review","volume":"6","author":"Balouiri","year":"2016","journal-title":"J. Pharm. Anal."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1016\/j.copbio.2011.08.007","article-title":"Polyphenols as antimicrobial agents","volume":"23","author":"Daglia","year":"2012","journal-title":"Curr. Opin. Biotechnol."},{"key":"ref_37","first-page":"133","article-title":"Antimicrobial and antiprotozoal effect of sweet marjoram (Origanum majorana L.)","volume":"9","author":"Laudy","year":"2010","journal-title":"Acta Sci. Pol. Cultus"},{"key":"ref_38","first-page":"352","article-title":"Antimicrobial effect of Oregano (Origanum majorana L.) extract on food-borne pathogens","volume":"21","author":"Choi","year":"2008","journal-title":"Korean J. Plant Resour."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1016\/j.indcrop.2012.08.029","article-title":"Evaluation of antioxidant activity, total phenols and phenolic compounds in thyme (Thymus vulgaris L.), sage (Salvia officinalis L.), and marjoram (Origanum majorana L.) extracts","volume":"43","author":"Roby","year":"2013","journal-title":"Ind. Crops Prod."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1016\/S0308-8146(01)00233-3","article-title":"Antioxidant effects of Origanum majorana L. on superoxide anion radicals","volume":"75","author":"Jun","year":"2001","journal-title":"Food Chem."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.advms.2017.07.003","article-title":"Cytotoxic, genotoxic and antimicrobial activity of caffeic and rosmarinic acids and their lithium, sodium and potassium salts as potential anticancer compounds","volume":"63","author":"Matejczyk","year":"2018","journal-title":"Adv. Med. Sci."},{"key":"ref_42","first-page":"183","article-title":"In vitro antimicrobial activity of some phenolic compounds (coumarin and quercetin) against gastroenteritis bacterial strains","volume":"3","author":"Nitiema","year":"2012","journal-title":"Int. J. Microbiol. Res."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1016\/j.apsusc.2014.03.125","article-title":"Antimicrobial mechanism of flavonoids against Escherichia coli ATCC 25922 by model membrane study","volume":"305","author":"He","year":"2014","journal-title":"Appl. Surf. Sci."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Zhang, M., Swarts, S.G., Yin, L., Liu, C., Tian, Y., Cao, Y., Swarts, M., Yang, S., Zhang, S.B., and Zhang, K. (2011). Antioxidant properties of quercetin. Oxygen Transport to Tissue XXXII, Springer.","DOI":"10.1007\/978-1-4419-7756-4_38"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1002\/ptr.5256","article-title":"Antioxidant and anti-inflammatory properties of the citrus flavonoids hesperidin and hesperetin: An updated review of their molecular mechanisms and experimental models","volume":"29","author":"Parhiz","year":"2015","journal-title":"Phyther. Res."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Khojasteh, A., Mirjalili, M.H., Alcalde, M.A., Cusido, R.M., Eibl, R., and Palazon, J. (2020). Powerful plant antioxidants: A new biosustainable approach to the production of rosmarinic acid. Antioxidants, 9.","DOI":"10.3390\/antiox9121273"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"2379","DOI":"10.1002\/jps.22446","article-title":"Preparation and characterization of quercetin nanocrystals","volume":"100","author":"Sahoo","year":"2011","journal-title":"J. Pharm. Sci."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1007\/s11483-012-9266-z","article-title":"Polymeric nanoparticles as oral delivery systems for encapsulation and release of polyphenolic compounds: Impact on quercetin antioxidant activity & bioaccessibility","volume":"7","author":"Pool","year":"2012","journal-title":"Food Biophys."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1517\/17425247.2012.679926","article-title":"Liposomal quercetin: Evaluating drug delivery in vitro and biodistribution in vivo","volume":"9","author":"Gang","year":"2012","journal-title":"Expert Opin. Drug Deliv."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1007\/s10973-010-1083-3","article-title":"Quercetin-PVP K25 solid dispersions: Preparation, thermal characterization and antioxidant activity","volume":"104","author":"Rocha","year":"2011","journal-title":"J. Therm. Anal. Calorim."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"638","DOI":"10.1016\/j.lwt.2015.11.004","article-title":"Morphological and in vitro antibacterial efficacy of quercetin loaded nanoparticles against food-borne microorganisms","volume":"66","author":"Kumar","year":"2016","journal-title":"LWT-Food Sci. Technol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1038\/nnano.2009.235","article-title":"Diagnosing lung cancer in exhaled breath using gold nanoparticles","volume":"4","author":"Peng","year":"2009","journal-title":"Nat. Nanotechnol."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Tzarouchis, D., and Sihvola, A. (2018). Light scattering by a dielectric sphere: Perspectives on the Mie resonances. Appl. Sci., 8.","DOI":"10.3390\/app8020184"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"2907","DOI":"10.1038\/s41598-018-21331-y","article-title":"Gold nanoparticles-enabled efficient dual delivery of anticancer therapeutics to HeLa cells","volume":"8","author":"Farooq","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"34","DOI":"10.32607\/20758251-2011-3-2-34-55","article-title":"Gold nanoparticles in biology and medicine: Recent advances and prospects","volume":"3","author":"Dykman","year":"2011","journal-title":"Acta Nat."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.trac.2014.07.017","article-title":"Nanoparticles as analytical tools for in-vitro antioxidant-capacity assessment and beyond","volume":"64","author":"Vilela","year":"2015","journal-title":"TRAC Trends Anal. Chem."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.colsurfb.2010.05.027","article-title":"Development of azithromycin\u2013PLGA nanoparticles: Physicochemical characterization and antibacterial effect against Salmonella typhi","volume":"80","author":"Mohammadi","year":"2010","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1093","DOI":"10.1016\/j.lwt.2014.05.061","article-title":"Synthesis, characterization of nisin loaded alginate\u2013chitosan\u2013pluronic composite nanoparticles and evaluation against microbes","volume":"59","author":"Bernela","year":"2014","journal-title":"LWT-Food Sci. Technol."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Hifnawy, M.S., Aboseada, M.A., Hassan, H.M., AboulMagd, A.M., Tohamy, A.F., Abdel-Kawi, S.H., Rateb, M.E., El Naggar, E.M.B., Liu, M., and Quinn, R.J. (2020). Testicular caspase-3 and \u03b2-Catenin regulators predicted via comparative metabolomics and docking studies. Metabolites, 10.","DOI":"10.3390\/metabo10010031"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"39203","DOI":"10.1038\/srep39203","article-title":"High-throughput and automated diagnosis of antimicrobial resistance using a cost-effective cellphone-based micro-plate reader","volume":"6","author":"Feng","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"37","DOI":"10.12816\/0046227","article-title":"Biofilm formation and antimicrobial resistance pattern of uropathogenic E. coli: Comparison of phenotypic and molecular methods","volume":"26","author":"Makled","year":"2017","journal-title":"Egypt. J. Med. Microbiol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1007\/s12161-011-9306-1","article-title":"Determination of free radical scavenging activity of plant extracts through DPPH assay: An EPR and UV\u2013Vis study","volume":"5","author":"Sanna","year":"2012","journal-title":"Food Anal. Methods"}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/11\/14\/1871\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:53:16Z","timestamp":1760140396000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/11\/14\/1871"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7,18]]},"references-count":62,"journal-issue":{"issue":"14","published-online":{"date-parts":[[2022,7]]}},"alternative-id":["plants11141871"],"URL":"https:\/\/doi.org\/10.3390\/plants11141871","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,7,18]]}}}