{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T15:54:26Z","timestamp":1770911666313,"version":"3.50.1"},"reference-count":65,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2022,8,30]],"date-time":"2022-08-30T00:00:00Z","timestamp":1661817600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32102373"],"award-info":[{"award-number":["32102373"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["6212030"],"award-info":[{"award-number":["6212030"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["2021ZD0001"],"award-info":[{"award-number":["2021ZD0001"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["CARS-23-A14"],"award-info":[{"award-number":["CARS-23-A14"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004826","name":"Beijing Natural Science Foundation","doi-asserted-by":"publisher","award":["32102373"],"award-info":[{"award-number":["32102373"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004826","name":"Beijing Natural Science Foundation","doi-asserted-by":"publisher","award":["6212030"],"award-info":[{"award-number":["6212030"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004826","name":"Beijing Natural Science Foundation","doi-asserted-by":"publisher","award":["2021ZD0001"],"award-info":[{"award-number":["2021ZD0001"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004826","name":"Beijing Natural Science Foundation","doi-asserted-by":"publisher","award":["CARS-23-A14"],"award-info":[{"award-number":["CARS-23-A14"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Inner Mongolia Autonomous Region","award":["32102373"],"award-info":[{"award-number":["32102373"]}]},{"name":"Inner Mongolia Autonomous Region","award":["6212030"],"award-info":[{"award-number":["6212030"]}]},{"name":"Inner Mongolia Autonomous Region","award":["2021ZD0001"],"award-info":[{"award-number":["2021ZD0001"]}]},{"name":"Inner Mongolia Autonomous Region","award":["CARS-23-A14"],"award-info":[{"award-number":["CARS-23-A14"]}]},{"DOI":"10.13039\/501100012453","name":"China Agricultural Research System","doi-asserted-by":"publisher","award":["32102373"],"award-info":[{"award-number":["32102373"]}],"id":[{"id":"10.13039\/501100012453","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012453","name":"China Agricultural Research System","doi-asserted-by":"publisher","award":["6212030"],"award-info":[{"award-number":["6212030"]}],"id":[{"id":"10.13039\/501100012453","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012453","name":"China Agricultural Research System","doi-asserted-by":"publisher","award":["2021ZD0001"],"award-info":[{"award-number":["2021ZD0001"]}],"id":[{"id":"10.13039\/501100012453","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012453","name":"China Agricultural Research System","doi-asserted-by":"publisher","award":["CARS-23-A14"],"award-info":[{"award-number":["CARS-23-A14"]}],"id":[{"id":"10.13039\/501100012453","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Brassica rapa is one of the most important leafy vegetables worldwide, and has a long history of cultivation. However, it has not been possible to completely control the damage of turnip mosaic virus (TuMV), a serious virus in B. rapa, to production. In this study, the genome-wide identification and expression detection of eIF family genes from B. rapa in response to TuMV resistance were analyzed, including the identification of eIF family genes, chromosomal distribution, three-dimensional (3D) structure and sequence logo analyses, and the expression characterization as well as differential metabolite analysis of eIF family genes in resistant\/susceptible lines, which may further prove the whole-genome tripling (WGT) event in B. rapa evolution and provide evidence for the functional redundancy and functional loss of multicopy eIF genes in evolution. A qRT-PCR analysis revealed that the relative expressions of eIF genes in a susceptible line (80461) were higher than those in a resistant line (80124), which may prove that, when TuMV infects host plants, the eIF genes can combine with the virus mRNA 5\u2032 end cap structure and promote the initiation of virus mRNA translation in the susceptible B. rapa line. In addition, the metabolite substances were detected, the differences in metabolites between disease-resistant and disease-susceptible plants were mainly manifested by altered compounds such as flavonoids, jasmonic acid, salicylic acid, ketones, esters, etc., which inferred that the different metabolite regulations of eIF family genes and reveal the resistance mechanisms of eIF genes against TuMV in brassica crops. This study may lay a new theoretical foundation for revealing eIF family gene resistance to TuMV in B. rapa, as well as advancing our understanding of virus\u2013host interactions.<\/jats:p>","DOI":"10.3390\/plants11172248","type":"journal-article","created":{"date-parts":[[2022,8,31]],"date-time":"2022-08-31T00:13:56Z","timestamp":1661904836000},"page":"2248","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Genome-Wide Identification and Expression Analysis of eIF Family Genes from Brassica rapa in Response to TuMV Resistance"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1395-6872","authenticated-orcid":false,"given":"Wenyue","family":"Huang","sequence":"first","affiliation":[{"name":"Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Zhongguancun, Nandajie No. 12, Haidian District, Beijing 100081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shaoxing","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Zhongguancun, Nandajie No. 12, Haidian District, Beijing 100081, China"},{"name":"College of Horticulture, Vegetable Genetics and Breeding Laboratory, Anhui Agricultural University, Changjiang West Road, No. 130, Hefei 230036, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shifan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Zhongguancun, Nandajie No. 12, Haidian District, Beijing 100081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fei","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Zhongguancun, Nandajie No. 12, Haidian District, Beijing 100081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Zhongguancun, Nandajie No. 12, Haidian District, Beijing 100081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4010-0562","authenticated-orcid":false,"given":"Rifei","family":"Sun","sequence":"additional","affiliation":[{"name":"Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Zhongguancun, Nandajie No. 12, Haidian District, Beijing 100081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shujiang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Zhongguancun, Nandajie No. 12, Haidian District, Beijing 100081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3294-7496","authenticated-orcid":false,"given":"Guoliang","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Zhongguancun, Nandajie No. 12, Haidian District, Beijing 100081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e0176","DOI":"10.1199\/tab.0176","article-title":"Mechanism of cytoplasmic mRNA translation","volume":"13","author":"Browning","year":"2015","journal-title":"Arabidopsis Book"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Joshi, B., Lee, K., Maeder, D.L., and Jagus, R. (2005). Phylogenetic analysis of eIF4E-family members. BMC Evol. Biol., 5.","DOI":"10.1186\/1471-2148-5-48"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1844","DOI":"10.1104\/pp.109.138438","article-title":"Evidence for variation in the optimal translation initiation complex: Plant eIF4B, eIF4F, and eIF(iso)4F differentially promote translation of mRNAs","volume":"150","author":"Mayberry","year":"2009","journal-title":"Plant Physiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"17742","DOI":"10.1074\/jbc.M109.009001","article-title":"Intrinsic RNA binding by the eukaryotic initiation factor 4F depends on a minimal RNA length but not on the m7G cap","volume":"284","author":"Kaye","year":"2009","journal-title":"J. Biol. Chem."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"16711","DOI":"10.1074\/jbc.R900002200","article-title":"eIF4E: New family members, new binding partners, new roles","volume":"284","author":"Rhoads","year":"2009","journal-title":"J. Biol. Chem."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"865","DOI":"10.1016\/j.mod.2005.04.002","article-title":"Functional diversity of the eukaryotic translation initiation factors belonging to eIF4 families","volume":"122","author":"Hernandez","year":"2005","journal-title":"Mech. Dev."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"922","DOI":"10.1038\/nature05603","article-title":"eIF4E function in somatic cells modulates ageing in Caenorhabditis elegans","volume":"445","author":"Syntichaki","year":"2007","journal-title":"Nature"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"3392","DOI":"10.3390\/v7072778","article-title":"Plant Translation Factors and Virus Resistance","volume":"7","author":"Sanfacon","year":"2015","journal-title":"Viruses"},{"key":"ref_9","first-page":"287812","article-title":"The eIF4F and eIFiso4F Complexes of Plants: An Evolutionary Perspective","volume":"2012","author":"Patrick","year":"2012","journal-title":"Comp. Func.t Genom."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/S0076-6879(07)29013-5","article-title":"Approaches for Analyzing the Differential Activities and Functions of eIF4E Family Members","volume":"429","author":"Rhoads","year":"2007","journal-title":"Methods Enzymol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1167","DOI":"10.1016\/j.febslet.2004.12.086","article-title":"Selective involvement of members of the eukaryotic initiation factor 4E family in the infection of Arabidopsis thaliana by potyviruses","volume":"579","author":"Sato","year":"2005","journal-title":"Febs Lett."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1498","DOI":"10.1094\/MPMI-05-10-0104","article-title":"Turnip mosaic virus (TuMV) Is Able to Use Alleles of Both eIF4E and eIF(iso)4E from Multiple Loci of the Diploid Brassica rapa","volume":"23","author":"Jenner","year":"2010","journal-title":"Mol. Plant-Microbe Interact."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1046","DOI":"10.1016\/S0960-9822(02)00898-9","article-title":"Loss-of-susceptibility mutants of Arabidopsis thaliana reveal an essential role for eIF(iso)4E during potyvirus infection","volume":"12","author":"Lellis","year":"2002","journal-title":"Curr. Biol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"6102","DOI":"10.1128\/JVI.78.12.6102-6111.2004","article-title":"The Arabidopsis Cucumovirus multiplication 1 and 2 loci encode tip translation initiation factors 4E and 4G","volume":"78","author":"Yoshii","year":"2004","journal-title":"J. Virol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1111\/mpp.12120","article-title":"Transgenic Brassica rapa plants over-expressing eIF(iso)4E variants show broad-spectrum Turnip mosaic virus (TuMV) resistance","volume":"15","author":"Kim","year":"2014","journal-title":"Mol. Plant Pathol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1111\/tpj.12389","article-title":"Multiple copies of eukaryotic translation initiation factors in Brassica rapa facilitate redundancy, enabling diversification through variation in splicing and broad-spectrum virus resistance","volume":"77","author":"Nellist","year":"2014","journal-title":"Plant J."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"3177","DOI":"10.1099\/vir.0.83194-0","article-title":"Genetic control of broad-spectrum resistance to turnip mosaic virus in Brassica rapa (Chinese cabbage)","volume":"88","author":"Rusholme","year":"2007","journal-title":"J. General Virol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2808","DOI":"10.1099\/vir.0.013151-0","article-title":"Allele mining in the pepper gene pool provided new complementation effects between pvr2-eIF4E and pvr6-eIF(iso)4E alleles for resistance to pepper veinal mottle virus","volume":"90","author":"Rubio","year":"2009","journal-title":"J. General Virol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1594","DOI":"10.1099\/vir.0.82702-0","article-title":"Molecular dissection of the potato virus YVPg virulence factor reveals complex adaptations to the pvr2 resistance allelic series in pepper","volume":"88","author":"Ayme","year":"2007","journal-title":"J. General Virol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1094\/MPMI-19-0557","article-title":"Different mutations in the genome-linked protein VPg of Potato virus Y confer virulence on the pvr2(3) resistance in pepper","volume":"19","author":"Ayme","year":"2006","journal-title":"Mol. Plant-Microbe Interact."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1094\/MPMI.2004.17.3.322","article-title":"Mutations in Potato virus Y genome-linked protein determine virulence toward recessive resistances in Capsicum annuum and Lycopersicon hirsutum","volume":"17","author":"Moury","year":"2004","journal-title":"Mol. Plant-Microbe Interact."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1007\/s00122-009-1169-0","article-title":"Non-synonymous single nucleotide polymorphisms in the watermelon eIF4E gene are closely associated with resistance to Zucchini yellow mosaic virus","volume":"120","author":"Ling","year":"2009","journal-title":"Theor. Appl. Genet."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1061","DOI":"10.1007\/s00122-014-2279-x","article-title":"Genetic analyses of BaMMV\/BaYMV resistance in barley accession HOR4224 result in the identification of an allele of the translation initiation factor 4e (Hv-eIF4E) exclusively effective against Barley mild mosaic virus (BaMMV)","volume":"127","author":"Perovic","year":"2014","journal-title":"Theor. Appl. Genet."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"7601","DOI":"10.1128\/JVI.00209-08","article-title":"Mutational analysis of plant cap-binding protein eIF4E reveals key amino acids involved in biochemical functions and potyvirus infection","volume":"82","author":"Walter","year":"2008","journal-title":"J. Virol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1094\/MPMI-23-1-0029","article-title":"Single Nucleotide Polymorphisms in a Gene for Translation Initiation Factor (eIF4G) of Rice (Oryza sativa) Associated with Resistance to Rice tungro spherical virus","volume":"23","author":"Lee","year":"2010","journal-title":"Mol. Plant-Microbe Interact."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2849","DOI":"10.1007\/s00122-013-2176-8","article-title":"A series of eIF4E alleles at the Bc-3 locus are associated with recessive resistance to Clover yellow vein virus in common bean","volume":"126","author":"Hart","year":"2013","journal-title":"Theor. Appl. Genet."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Konecna, E., Safarova, D., Navratil, M., Hanacek, P., Coyne, C., Flavell, A., Vishnyakova, M., Ambrose, M., Redden, R., and Smykal, P. (2014). Geographical Gradient of the eIF4E Alleles Conferring Resistance to Potyviruses in Pea (Pisum) Germplasm. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0090394"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1111\/j.1364-3703.2009.00535.x","article-title":"Quantitative trait loci meta-analysis of Plum pox virus resistance in apricot (Prunus armeniaca L.): New insights on the organization and the identification of genomic resistance factors","volume":"10","author":"Marandel","year":"2009","journal-title":"Mol. Plant Pathol."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Wang, X.H., Kohalmi, S.E., Svircev, A., Wang, A.M., Sanfacon, H., and Tian, L.N. (2013). Silencing of the Host Factor eIF(iso)4E Gene Confers Plum Pox Virus Resistance in Plum. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0050627"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1099\/vir.0.057810-0","article-title":"Alternative mutational pathways, outside the VPg, of rice yellow mottle virus to overcome eIF(iso)4G-mediated rice resistance under strong genetic constraints","volume":"95","author":"Poulicard","year":"2014","journal-title":"J. General Virol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1007\/s00122-010-1300-2","article-title":"Identification of a second major resistance gene to Rice yellow mottle virus, RYMV2, in the African cultivated rice species, O. glaberrima","volume":"121","author":"Thiemele","year":"2010","journal-title":"Theor. Appl. Genet."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.virol.2010.09.007","article-title":"The adaptation of Rice yellow mottle virus to the eIF(iso)4G-mediated rice resistance","volume":"408","author":"Traore","year":"2010","journal-title":"Virology"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1007\/s00438-005-0003-x","article-title":"The recessive potyvirus resistance gene pot-1 is the tomato orthologue of the pepper pvr2-eIF4E gene","volume":"274","author":"Ruffel","year":"2005","journal-title":"Mol. Genet. Genom."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"36951","DOI":"10.1074\/jbc.M103869200","article-title":"eIF4G functionally differs from eIFiso4G in promoting internal initiation, cap-independent translation, and translation of structured mRNAs","volume":"276","author":"Gallie","year":"2001","journal-title":"J. Biol. Chem."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1149","DOI":"10.1007\/s001220051319","article-title":"Characterisation of resistance to turnip mosaic virus in oilseed rape (Brassica napus) and genetic mapping of TuRB01","volume":"99","author":"Walsh","year":"1999","journal-title":"Theor. Appl. Genet."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1169","DOI":"10.1007\/s00122-003-1363-4","article-title":"Genetic mapping of the novel Turnip mosaic virus resistance gene TuRB03 in Brassica napus","volume":"107","author":"Hughes","year":"2003","journal-title":"Theor. Appl. Genet."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1006\/viro.2002.1519","article-title":"Mutations in Turnip mosaic virus P3 and Cylindrical Inclusion Proteins Are Separately Required to Overcome Two Brassica napus Resistance Genes","volume":"300","author":"Jenner","year":"2002","journal-title":"Virology"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1094\/MPMI.2003.16.9.777","article-title":"The dual role of the potyvirus P3 protein of Turnip mosaic virus as a symptom and avirulence determinant in brassicas","volume":"16","author":"Jenner","year":"2003","journal-title":"Mol. Plant Microbe Interact."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1007\/s00122-012-1972-x","article-title":"Mapping and candidate-gene screening of the novel Turnip mosaic virus resistance gene retr02 in Chinese cabbage (Brassica rapa L.)","volume":"126","author":"Qian","year":"2012","journal-title":"Theor. Appl. Genet."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1111\/tpj.13519","article-title":"Eukaryotic translation initiation factor 2B-beta (eIF2B\u03b2), a new class of plant virus resistance gene","volume":"90","author":"Shopan","year":"2017","journal-title":"Plant J."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"47","DOI":"10.5423\/PPJ.OA.07.2020.0125","article-title":"Variability in the Viral Protein Linked to the Genome of Turnip Mosaic Virus Influences Interactions with eIF(iso)4Es in Brassica rapa","volume":"37","author":"Li","year":"2021","journal-title":"The Plant Pathol. J."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Li, G.L., Qian, W., Zhang, S.J., Zhang, S.F., Li, F., Zhang, H., Fang, Z.Y., Wu, J., Wang, X.W., and Sun, R.F. (2018). Variability in eukaryotic initiation factor iso4E in Brassica rapa influences interactions with the viral protein linked to the genome of Turnip mosaic virus. Sci. Rep., 8.","DOI":"10.1038\/s41598-018-31739-1"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1035","DOI":"10.1038\/ng.919","article-title":"The genome of the mesopolyploid crop species Brassica rapa","volume":"43","author":"Wang","year":"2011","journal-title":"Nat. Genet."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1038\/nature14891","article-title":"Structure of mammalian eIF3 in the context of the 43S preinitiation complex","volume":"525","author":"Dhote","year":"2015","journal-title":"Nature"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1177","DOI":"10.1002\/embj.201387344","article-title":"e IF 5 B employs a novel domain release mechanism to catalyze ribosomal subunit joining","volume":"33","author":"Kuhle","year":"2014","journal-title":"EMBO J."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"25254","DOI":"10.1074\/jbc.274.36.25254","article-title":"Importance of Redox Potential for the in Vivo Function of the Cytoplasmic Disulfide Reductant Thioredoxin from Escherichia coli","volume":"274","author":"Rietsch","year":"1999","journal-title":"J. Biol. Chem."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"3148","DOI":"10.1073\/pnas.0808275106","article-title":"Crystal structure of the eIF4A\u2013PDCD4 complex","volume":"106","author":"Chang","year":"2009","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Hern\u00e1ndez, G., and Jagus, R. (2016). The Diversification of eIF4E Family Members in Plants and Their Role in the Plant-Virus Interaction. Evolution of the Protein Synthesis Machinery and Its Regulation, Springer International Publishing.","DOI":"10.1007\/978-3-319-39468-8"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1111\/j.1365-313X.2004.02215.x","article-title":"The potyvirus recessive resistance gene, sbm1, identifies a novel role for translation initiation factor eIF4E in cell-to-cell trafficking","volume":"40","author":"Gao","year":"2004","journal-title":"Plant J."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"2913","DOI":"10.1105\/tpc.107.050997","article-title":"Functional Dissection of Naturally Occurring Amino Acid Substitutions in eIF4E That Confers Recessive Potyvirus Resistance in Plants","volume":"19","author":"Yeam","year":"2007","journal-title":"Plant Cell"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1272","DOI":"10.1104\/pp.102.017855","article-title":"The eukaryotic translation initiation factor 4E controls lettuce susceptibility to the potyvirus Lettuce mosaic virus","volume":"132","author":"Nicaise","year":"2003","journal-title":"Plant Physiol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"927","DOI":"10.1046\/j.1365-313X.2002.01481.x","article-title":"The Arabidopsis eukaryotic initiation factor (iso)4E is dispensable for plant growth but required for susceptibility to potyviruses","volume":"32","author":"Duprat","year":"2002","journal-title":"Plant J."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1007\/s10059-009-0042-y","article-title":"Double mutations in eIF4E and eIFiso4E confer recessive resistance to Chilli veinal mottle virus in pepper","volume":"27","author":"Hwang","year":"2009","journal-title":"Mol. Cells"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1111\/j.1364-3703.2009.00602.x","article-title":"Potyviral resistance derived from cultivars of Phaseolus vulgaris carrying bc-3 is associated with the homozygotic presence of a mutated eIF4E allele","volume":"11","author":"Naderpour","year":"2010","journal-title":"Mol. Plant Pathol."},{"key":"ref_55","first-page":"3653","article-title":"An exceptionally high nucleotide and haplotype diversity and a signature of positive selection for the eIF4E resistance gene in barley are revealed by allele mining and phylogenetic analyses of natural populations","volume":"20","author":"Hofinger","year":"2011","journal-title":"Mol. Ecol."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Piron, F., Nicola\u00ef, M., Mino\u00efa, S., Piednoir, E., Moretti, A., Salgues, A., Zamir, D., Caranta, C., and Bendahmane, A. (2010). An induced mutation in tomato eIF4E leads to immunity to two potyviruses. PLoS ONE, 5.","DOI":"10.1371\/journal.pone.0011313"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1007\/s11248-011-9576-9","article-title":"Overexpression of the wild potato eIF4E-1 variant Eva1 elicits Potato virus Y resistance in plants silenced for native eIF4E-1","volume":"21","author":"Duan","year":"2011","journal-title":"Transgenic Res."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1111\/j.1365-313X.2005.02381.x","article-title":"The pvr1 locus in Capsicum encodes a translation initiation factor eIF4E that interacts with Tobacco etch virus VPg","volume":"42","author":"Kang","year":"2005","journal-title":"Plant J."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1035","DOI":"10.1111\/ppa.13016","article-title":"TuMV management for brassica crops through host resistance: Retrospect and prospects","volume":"68","author":"Li","year":"2019","journal-title":"Plant Pathol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"W200","DOI":"10.1093\/nar\/gky448","article-title":"HMMER web server: 2018 update","volume":"46","author":"Potter","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"W242","DOI":"10.1093\/nar\/gkw290","article-title":"Interactive tree of life (iTOL) v3: An online tool for the display and annotation of phylogenetic and other trees","volume":"44","author":"Letunic","year":"2016","journal-title":"Nucleic Acids Res."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1870","DOI":"10.1093\/molbev\/msw054","article-title":"MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets","volume":"33","author":"Kumar","year":"2016","journal-title":"Molecular Biol. Evol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1194","DOI":"10.1016\/j.molp.2020.06.009","article-title":"TBtools: An Integrative Toolkit Developed for Interactive Analyses of Big Biological Data","volume":"13","author":"Chen","year":"2020","journal-title":"Mol. Plant"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1093\/nar\/30.1.325","article-title":"PlantCARE, a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences","volume":"30","author":"Lescot","year":"2002","journal-title":"Nucleic Acids Res."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"1188","DOI":"10.1101\/gr.849004","article-title":"WebLogo: A sequence logo generator","volume":"14","author":"Crooks","year":"2004","journal-title":"Genome Res."}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/11\/17\/2248\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:20:00Z","timestamp":1760142000000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/11\/17\/2248"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8,30]]},"references-count":65,"journal-issue":{"issue":"17","published-online":{"date-parts":[[2022,9]]}},"alternative-id":["plants11172248"],"URL":"https:\/\/doi.org\/10.3390\/plants11172248","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,8,30]]}}}