{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T14:18:43Z","timestamp":1790777923188,"version":"4.1.0"},"reference-count":54,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2022,8,31]],"date-time":"2022-08-31T00:00:00Z","timestamp":1661904000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Laboratoire de G\u00e9nie Chimique, Universit\u00e9 de Toulouse, CNRS, INPT, UPS, Toulouse, France"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Treatment of biofilm-associated infections has become a major challenge in biomedical and clinical fields due to the failure of conventional treatments in controlling this highly complex and tolerant structure. Therefore, the search for novel antibiofilm agents with increased efficacy as those provided by natural products, presents an urgent need. The aim of this study was to explore extracts derived from three algae (green Ulva lactuca, brown Stypocaulon scoparium, red Pterocladiella capillacea) for their potential antibiofilm activity against Staphylococcus aureus, bacterium responsible for several acute and chronic infections. Seaweed extracts were prepared by successive maceration in various solvents (cyclohexane (CH), dichloromethane (DCM), ethyl acetate (EA), and methanol (MeOH)). The ability of the different extracts to inhibit S. aureus biofilm formation was assessed using colony-forming unit (CFU) counts method supported by epifluorescence microscopic analysis. Effects of active extracts on the biofilm growth cycle, as well as on S. aureus surface hydrophobicity were evaluated. Results revealed the ability of four extracts to significantly inhibit S. aureus biofilm formation. These findings were supported by microscopy analyses. The gradual increase in the number of adherent bacteria when the selected extracts were added at various times (t0, t2h, t4h, t6h, and t24h) revealed their potential effect on the initial adhesion and proliferation stages of S. aureus biofilm development. Interestingly, a significant reduction in the surface hydrophobicity of S. aureus treated with dichloromethane (DCM) extract derived from U. lactuca was demonstrated. These findings present new insights into the exploration of seaweeds as a valuable source of antibiofilm agents with preventive effect by inhibiting and\/or delaying biofilm formation.<\/jats:p>","DOI":"10.3390\/plants11172285","type":"journal-article","created":{"date-parts":[[2022,9,1]],"date-time":"2022-09-01T03:55:38Z","timestamp":1662004538000},"page":"2285","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Seaweed Extracts as an Effective Gateway in the Search for Novel Antibiofilm Agents against Staphylococcus aureus"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4211-2583","authenticated-orcid":false,"given":"Maya","family":"Rima","sequence":"first","affiliation":[{"name":"Laboratoire de G\u00e9nie Chimique, Universit\u00e9 de Toulouse, CNRS, INPT, UPS, 31062 Toulouse, France"},{"name":"Laboratory of Applied Biotechnology, AZM Center for Research in Biotechnology and Its Applications, Doctoral School of Science and Technology, Lebanese University, El Mittein Street, Tripoli 1300, Lebanon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asma","family":"Chbani","sequence":"additional","affiliation":[{"name":"Laboratory of Applied Biotechnology, AZM Center for Research in Biotechnology and Its Applications, Doctoral School of Science and Technology, Lebanese University, El Mittein Street, Tripoli 1300, Lebanon"},{"name":"Faculty of Public Health III, Lebanese University, Tripoli 1300, Lebanon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7505-1000","authenticated-orcid":false,"given":"Christine","family":"Roques","sequence":"additional","affiliation":[{"name":"Laboratoire de G\u00e9nie Chimique, Universit\u00e9 de Toulouse, CNRS, INPT, UPS, 31062 Toulouse, France"},{"name":"Bacteriology-Hygiene Department, Centre Hospitalier Universitaire, H\u00f4pital Purpan, 31300 Toulouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7217-5879","authenticated-orcid":false,"given":"Fatima","family":"El Garah","sequence":"additional","affiliation":[{"name":"Laboratoire de G\u00e9nie Chimique, Universit\u00e9 de Toulouse, CNRS, INPT, UPS, 31062 Toulouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1234","DOI":"10.1055\/s-0034-1383001","article-title":"Exploring marine resources for bioactive compounds","volume":"80","author":"Kiuru","year":"2014","journal-title":"Planta Med."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"959","DOI":"10.1021\/acsmedchemlett.8b00368","article-title":"Marine Natural Products in Medicinal Chemistry","volume":"9","author":"Jimenez","year":"2018","journal-title":"ACS Med. 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