{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T23:19:06Z","timestamp":1789427946489,"version":"build-2803163510"},"reference-count":39,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2022,9,11]],"date-time":"2022-09-11T00:00:00Z","timestamp":1662854400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31770752"],"award-info":[{"award-number":["31770752"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["BRA2018065"],"award-info":[{"award-number":["BRA2018065"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["BM2013478"],"award-info":[{"award-number":["BM2013478"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Fifth Stage Funded Research Projects of 333 in Jiangsu Province","award":["31770752"],"award-info":[{"award-number":["31770752"]}]},{"name":"Fifth Stage Funded Research Projects of 333 in Jiangsu Province","award":["BRA2018065"],"award-info":[{"award-number":["BRA2018065"]}]},{"name":"Fifth Stage Funded Research Projects of 333 in Jiangsu Province","award":["BM2013478"],"award-info":[{"award-number":["BM2013478"]}]},{"name":"Scientific and Technological Suppot Project of Jiangsu Province","award":["31770752"],"award-info":[{"award-number":["31770752"]}]},{"name":"Scientific and Technological Suppot Project of Jiangsu Province","award":["BRA2018065"],"award-info":[{"award-number":["BRA2018065"]}]},{"name":"Scientific and Technological Suppot Project of Jiangsu Province","award":["BM2013478"],"award-info":[{"award-number":["BM2013478"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Bougainvillea plants have important ornamental and cultural value, as well as applications, for example, in improving the ecological environment, sterilization and as anti-virals in medicines, etc. Among many varieties, Bougainvillea buttiana \u2018Miss Manila\u2019 is more popular in landscape applications in southern China because of its excellent qualities. However, because of the difficulty of sexual reproduction, low rooting rate of asexual propagation cuttings and low temperature tolerance, its large-scale development is limited. For easy, quick and mass multiplication of such cultivars, tissue culture technique can be put to use. In this study, nodal segments of \u2018Miss Manila\u2019 were used as explants, and a single-factor experiment was carried out with a variety of plant growth regulators and concentrations to establish and optimize a complete tissue culture propagation system. The results showed that the best disinfestation was 75% ethanol treatment for 0.5 min + 0.1% HgCl2 treatment for 9 min, and the survival rate was 86.83%. The best shoot initiation formula was MS + 6-BA (2.5 mg\/L) + IBA (0.2 mg\/L), and the induction rate was 95.14%. The best formula for shoot proliferation was MS + 6-BA (1.5 mg\/L) + NAA (0.1 mg\/L), and the proliferation coefficient was 3.13. The best rooting culture formula was 1\/2 MS + IBA (2.0 mg\/L), and the rooting rate was 81.97%. The survival rate for plantlet refining and transplanting was 93.33%. In this study, a relatively efficient regeneration system for the tissue culture and rapid propagation of Bougainvillea buttiana \u2018Miss Manila\u2019 was established to address the problems of rooting and transplanting of this variety, to benefit research on the industrialized production and regeneration systems of this variety, and to provide a reference for the tissue culture of other varieties of Bougainvillea or other woody plants.<\/jats:p>","DOI":"10.3390\/plants11182372","type":"journal-article","created":{"date-parts":[[2022,9,13]],"date-time":"2022-09-13T04:05:41Z","timestamp":1663041941000},"page":"2372","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Establishing an Efficient Regeneration System for Tissue Culture in Bougainvillea buttiana \u2018Miss Manila\u2019"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7396-6947","authenticated-orcid":false,"given":"Tao","family":"Huang","sequence":"first","affiliation":[{"name":"Collage of Landscape Architecture, Nanjing Forestry University, Nanjing 210037, China"},{"name":"Southern Modern Forestry Collaborative Innovation Center, Nanjing Forestry University, Nanjing 210037, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8778-8122","authenticated-orcid":false,"given":"Huihui","family":"Zhang","sequence":"additional","affiliation":[{"name":"Collage of Landscape Architecture, Nanjing Forestry University, Nanjing 210037, China"},{"name":"Southern Modern Forestry Collaborative Innovation Center, Nanjing Forestry University, Nanjing 210037, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7234-6380","authenticated-orcid":false,"given":"Runan","family":"Zhao","sequence":"additional","affiliation":[{"name":"Collage of Landscape Architecture, Nanjing Forestry University, Nanjing 210037, China"},{"name":"Southern Modern Forestry Collaborative Innovation Center, Nanjing Forestry University, Nanjing 210037, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zunling","family":"Zhu","sequence":"additional","affiliation":[{"name":"Collage of Landscape Architecture, Nanjing Forestry University, Nanjing 210037, China"},{"name":"Southern Modern Forestry Collaborative Innovation Center, Nanjing Forestry University, Nanjing 210037, China"},{"name":"College of Art and Design, Nanjing Forestry University, Nanjing 210037, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"856","DOI":"10.3732\/ajb.94.5.856","article-title":"Molecular phylogeny of Nyctaginaceae: Taxonomy, biogeography, and characters associated with a radiation of xerophytic genera in North America","volume":"94","author":"Douglas","year":"2007","journal-title":"Am. 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