{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T01:27:18Z","timestamp":1760232438016,"version":"build-2065373602"},"reference-count":46,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2022,11,4]],"date-time":"2022-11-04T00:00:00Z","timestamp":1667520000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003627","name":"Rural Development Administration","doi-asserted-by":"publisher","award":["PJ01335801","NRF-2020R1A2C4002294","NRF-2017M3A9G6068245","NRF-2021R1C1C1012962"],"award-info":[{"award-number":["PJ01335801","NRF-2020R1A2C4002294","NRF-2017M3A9G6068245","NRF-2021R1C1C1012962"]}],"id":[{"id":"10.13039\/501100003627","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation, Ministry of Science, Technology, and Future Planning, Korea","doi-asserted-by":"publisher","award":["PJ01335801","NRF-2020R1A2C4002294","NRF-2017M3A9G6068245","NRF-2021R1C1C1012962"],"award-info":[{"award-number":["PJ01335801","NRF-2020R1A2C4002294","NRF-2017M3A9G6068245","NRF-2021R1C1C1012962"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"Korean government (MSIT)","doi-asserted-by":"publisher","award":["PJ01335801","NRF-2020R1A2C4002294","NRF-2017M3A9G6068245","NRF-2021R1C1C1012962"],"award-info":[{"award-number":["PJ01335801","NRF-2020R1A2C4002294","NRF-2017M3A9G6068245","NRF-2021R1C1C1012962"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Influenza viruses are a major public health threat that causes repetitive outbreaks. In recent years, genotype 4 (G4) reassortant Eurasian avian-like (EA) H1N1 (G4 EA H1N1) has garnered attention as a potential novel pandemic strain. The necessity of developing vaccines against G4 EA H1N1 is growing because of the increasing cases of human infection and the low cross-reactivity of the strain with current immunity. In this study, we produced a G4 EA H1N1-derived neuraminidase (G4NA) as a vaccine candidate in Nicotiana benthamiana. The expressed G4NA was designed to be accumulated in the endoplasmic reticulum (ER). The M-domain of the human receptor-type tyrosine-protein phosphatase C was incorporated into the expression cassette to enhance the translation of G4NA. In addition, the family 3 cellulose-binding module and Brachypodium distachyon small ubiquitin-like modifier sequences were used to enable the cost-effective purification and removal of unnecessary domains after purification, respectively. The G4NA produced in plants displayed high solubility and assembled as a tetramer, which is required for the efficacy of an NA-based vaccine. In a mouse immunization model, the G4NA produced in plants could induce significant humoral immune responses. The plant-produced G4NA also stimulated antigen-specific CD4 T cell activation. These G4NA vaccine-induced immune responses were intensified by the administration of the antigen with a vaccine adjuvant. These results suggest that G4NA produced in plants has great potential as a vaccine candidate against G4 EA H1N1.<\/jats:p>","DOI":"10.3390\/plants11212984","type":"journal-article","created":{"date-parts":[[2022,11,7]],"date-time":"2022-11-07T03:02:22Z","timestamp":1667790142000},"page":"2984","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["In planta Production and Validation of Neuraminidase Derived from Genotype 4 Reassortant Eurasian Avian-like H1N1 Virus as a Vaccine Candidate"],"prefix":"10.3390","volume":"11","author":[{"given":"Da Been","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Integrative Food, Bioscience and Biotechnology, Chonnam National University, Gwangju 61186, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8438-3365","authenticated-orcid":false,"given":"Sun Min","family":"Lee","sequence":"additional","affiliation":[{"name":"Viral Immunology Laboratory, Institut Pasteur Korea, Seongnam 13488, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyoung Rok","family":"Geem","sequence":"additional","affiliation":[{"name":"Department of Bioenergy Science and Technology, Chonnam National University, Gwangju 61186, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jitae","family":"Kim","sequence":"additional","affiliation":[{"name":"Bio-Energy Research Center, Chonnam National University, Gwangju 61186, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eui Ho","family":"Kim","sequence":"additional","affiliation":[{"name":"Viral Immunology Laboratory, Institut Pasteur Korea, Seongnam 13488, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong Wook","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Integrative Food, Bioscience and Biotechnology, Chonnam National University, Gwangju 61186, Korea"},{"name":"Department of Bioenergy Science and Technology, Chonnam National University, Gwangju 61186, Korea"},{"name":"Bio-Energy Research Center, Chonnam National University, Gwangju 61186, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"913","DOI":"10.15585\/mmwr.mm7129a1","article-title":"Influenza Activity and Composition of the 2022-23 Influenza Vaccine-United States, 2021\u20132022 Season","volume":"71","author":"Daly","year":"2022","journal-title":"MMWR. 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