{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T02:17:58Z","timestamp":1782440278500,"version":"3.54.5"},"reference-count":73,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2023,1,3]],"date-time":"2023-01-03T00:00:00Z","timestamp":1672704000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Scientific Grant Agency of the Ministry of Education, Science, Research and Sport of the Slovak Republic","award":["1\/0882\/21"],"award-info":[{"award-number":["1\/0882\/21"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>The weed Solidago canadensis L. poses a global threat to the environment as it spreads uncontrollably on roadsides, in forests, fields, meadows, and farmland. Goldenrod emits toxic substances that suppress other plants on the site, displacing wild ones. Thus, goldenrod conquers huge areas very quickly. The use of herbicides and mechanical methods does not solve the problem of the spontaneous spread of goldenrod. On the other hand, many scientists consider goldenrod as a valuable source of biologically active substances: flavonoids, phenolic compounds, vitamins, etc. In this study, we consider Solidago plants as a promising, free (cheap), and renewable substrate for the production of methane gas. The goal of the study was to identify the main patterns of degradation of the Solidago canadensis L. plant by methane-producing and sulfate-reducing bacteria with methane gas production and simultaneous detoxification of toxic copper. The composition of the gas phase was monitored by gas chromatography. The pH and redox potential parameters were determined potentiometrically; metal concentrations were measured by photometry. The concentration of flavonoids, sugars and phenolic compounds in plant biomass was determined according to well-known protocols. As a result of the study, high efficiencies of methane degradation in the Solidago plant and copper detoxification were obtained. Methane yield has reached the value of 68.2 L kg\u22121 TS of Solidago canadensis L. biomass. The degradation coefficient (Kd) was also high at 21.4. The Cu(II) was effectively immobilized by methanogens and sulfate reducers during the goldenrod degradation at the initial concentrations of 500 mg L\u22121. Thus, a new method of beneficial application of invasive plants was presented. The result confirms the possibility of using methanogenic microorganisms to produce methane gas from invasive weeds and detoxification of toxic metals.<\/jats:p>","DOI":"10.3390\/plants12010198","type":"journal-article","created":{"date-parts":[[2023,1,3]],"date-time":"2023-01-03T02:05:53Z","timestamp":1672711553000},"page":"198","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Anaerobic Degradation of the Invasive Weed Solidago canadensis L. (goldenrod) and Copper Immobilization by a Community of Sulfate-Reducing and Methane-Producing Bacteria"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2815-3976","authenticated-orcid":false,"given":"Olesia","family":"Havryliuk","sequence":"first","affiliation":[{"name":"Department of Extremophilic Microorganisms Biology, Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine, 03143 Kyiv, Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4265-5534","authenticated-orcid":false,"given":"Vira","family":"Hovorukha","sequence":"additional","affiliation":[{"name":"Department of Extremophilic Microorganisms Biology, Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine, 03143 Kyiv, Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7044-3339","authenticated-orcid":false,"given":"Iryna","family":"Bida","sequence":"additional","affiliation":[{"name":"Department of Extremophilic Microorganisms Biology, Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine, 03143 Kyiv, Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3855-1847","authenticated-orcid":false,"given":"Galyna","family":"Gladka","sequence":"additional","affiliation":[{"name":"Department of Extremophilic Microorganisms Biology, Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine, 03143 Kyiv, Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Artem","family":"Tymoshenko","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Faculty of Environmental Safety, Engineering and Technologies, National Aviation University, 03058 Kyiv, Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5420-6296","authenticated-orcid":false,"given":"Semen","family":"Kyrylov","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Faculty of Environmental Safety, Engineering and Technologies, National Aviation University, 03058 Kyiv, Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8464-4142","authenticated-orcid":false,"given":"Ruslan","family":"Mariychuk","sequence":"additional","affiliation":[{"name":"Department of Ecology, Faculty of Humanities and Natural Sciences, Presov Universityin Presov, 08116 Presov, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7698-5155","authenticated-orcid":false,"given":"Oleksandr","family":"Tashyrev","sequence":"additional","affiliation":[{"name":"Department of Extremophilic Microorganisms Biology, Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine, 03143 Kyiv, Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,1,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e202200728","DOI":"10.1002\/cbdv.202200728","article-title":"Selected Aspects of Invasive Solidago Canadensis with an Emphasis on Its Allelopathic Abilities: A Review","volume":"19","author":"Zhu","year":"2022","journal-title":"Chem. Biodivers."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1009","DOI":"10.15666\/aeer\/2002_10091022","article-title":"Allelopathic Effects of Aqueous Extracts from Different Plant Parts of Canada Goldenrod (Solidago canadensis L.) On Seed Germination and Seedling Growth of Korean Lawngrass (Zoysia japonica Steud.)","volume":"20","author":"Sun","year":"2022","journal-title":"Appl. Ecol. Environ. Res."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1985","DOI":"10.1002\/ldr.4278","article-title":"Restoration of Ecologically Valuable Grassland on Sites Degraded by Invasive Solidago: Lessons from a 6-year Experiment","volume":"33","author":"Szymura","year":"2022","journal-title":"Land Degrad. Dev."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1007\/s10340-018-1034-5","article-title":"Evaluation of Two Invasive Plant Invaders in Europe (Solidago canadensis and Solidago gigantea) as Possible Sources of Botanical Insecticides","volume":"92","author":"Benelli","year":"2019","journal-title":"J. Pest Sci."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Likhanov, A., Oliinyk, M., Pashkevych, N., Churilov, A., and Kozyr, M. (2021). The Role of Flavonoids in Invasion Strategy of Solidago canadensis L.. Plants, 10.","DOI":"10.3390\/plants10081748"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"587","DOI":"10.5219\/1378","article-title":"Assessment of Flavonoids and Phenolic Compound Accumulation in Invasive Solidago canadensis L. in Slovakia","volume":"14","author":"Shelepova","year":"2020","journal-title":"Potravinarstvo"},{"key":"ref_7","unstructured":"Sili, A. (2020, January 24\u201326). The Antioxidant profile of solidago species. Proceedings of the International Medical Congress for Students and Young Doctors, Chisinau, Moldova. Available online: https:\/\/ibn.idsi.md\/vizualizare_articol\/120972."},{"key":"ref_8","first-page":"174","article-title":"Biogas Production from Napier Grass and Cattle Slurry Using a Green Energy Technology","volume":"4","author":"Souvannasouk","year":"2021","journal-title":"Int. J. Innov. Res. Sci. Stud."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"280","DOI":"10.5219\/1125","article-title":"Invasive Solidago canadensis L. As A Resource of Valuable Biological Compounds","volume":"13","author":"Shelepova","year":"2019","journal-title":"Potravinarstvo"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"146208","DOI":"10.1016\/j.scitotenv.2021.146208","article-title":"An Evaluation of Influencing Factors and Public Attitudes for the Adoption of Biogas System in Rural Communities to Overcome Energy Crisis: A Case Study of Pakistan","volume":"778","author":"Luo","year":"2021","journal-title":"Sci. Total Environ."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"8243","DOI":"10.1016\/j.ijhydene.2018.03.099","article-title":"Utilization of Pistia stratiotes (Aquatic Weed) for Fermentative Biohydrogen: Electron-Equivalent Balance, Stoichiometry, and Cost Estimation","volume":"43","author":"Mthethwa","year":"2018","journal-title":"Int. J. Hydrog. Energy"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Saratale, R.G., Cho, S.-K., Ghodake, G.S., Shin, H.-S., Saratale, G.D., Park, Y., Lee, H.-S., Bharagava, R.N., and Kim, D.-S. (2020). Utilization of Noxious Weed Water Hyacinth Biomass as a Potential Feedstock for Biopolymers Production: A Novel Approach. Polymers, 12.","DOI":"10.3390\/polym12081704"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Gonz\u00e1lez, R., Pe\u00f1a, D.C., and G\u00f3mez, X. (2022). Anaerobic Co-Digestion of Wastes: Reviewing Current Status and Approaches for Enhancing Biogas Production. Appl. Sci., 12.","DOI":"10.3390\/app12178884"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Prakash, J., Sharma, R., Patel, S.K., Kim, I.-W., and Kalia, V.C. (2018). Bio-Hydrogen Production by Co-Digestion of Domestic Wastewater and Biodiesel Industry Effluent. PloS ONE, 13.","DOI":"10.1371\/journal.pone.0199059"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"22370","DOI":"10.1039\/C8RA03284J","article-title":"Biochar Preparation from Solidago canadensis and Its Alleviation of the Inhibition of Tomato Seed Germination by Allelochemicals","volume":"8","author":"Zhang","year":"2018","journal-title":"RSC Adv."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1043","DOI":"10.3390\/md12021043","article-title":"Co-Cultivation\u2014A Powerful Emerging Tool for Enhancing the Chemical Diversity of Microorganisms","volume":"12","author":"Marmann","year":"2014","journal-title":"Mar. Drugs"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.lssr.2021.09.004","article-title":"Lignocellulose Degradation, Biogas Production and Characteristics of the Microbial Community in Solid-State Anaerobic Digestion of Wheat Straw Waste","volume":"32","author":"Jin","year":"2022","journal-title":"Life Sci. Space Res."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"111491","DOI":"10.1016\/j.rser.2021.111491","article-title":"Integrating Strategies for Sustainable Conversion of Waste Biomass into Dark-Fermentative Hydrogen and Value-Added Products","volume":"150","author":"Patel","year":"2021","journal-title":"Renew. Sustain. Energy Rev."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1134\/S0026261720020101","article-title":"Syntrophy and Interspecies Electron Transfer in Methanogenic Microbial Communities","volume":"89","author":"Nozhevnikova","year":"2020","journal-title":"Microbiology"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"125538","DOI":"10.1016\/j.biortech.2021.125538","article-title":"Enhancing Hydrolysis and Syntropy Simultaneously in Solid State Anaerobic Digestion: Digester Performance and Techno-Economic Evaluation","volume":"338","author":"Paritosh","year":"2021","journal-title":"Bioresour. Technol."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Zhang, Z., Wen, G., Bu, D., Sun, G., and Qiang, S. (2022). Long\u2212Distance Wind Dispersal Drives Population Range Expansion of Solidago canadensis. Plants, 11.","DOI":"10.3390\/plants11202734"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Wiatrowska, B.M., Wawro, A., Gieparda, W., and Waliszewska, B. (2022). Bioethanol Production Potential and Other Biomass Energy Properties of Invasive Reynoutria, Solidago, and Spiraea Plants. Forests, 13.","DOI":"10.3390\/f13101582"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Havryliuk, O., Hovorukha, V., Savitsky, O., Trilis, V., Kalinichenko, A., Do\u0142ha\u0144czuk-\u015ar\u00f3dka, A., Janecki, D., and Tashyrev, O. (2021). Anaerobic Degradation of Environmentally Hazardous Aquatic Plant Pistia Stratiotes and Soluble Cu(II) Detoxification by Methanogenic Granular Microbial Preparation. Energies, 14.","DOI":"10.3390\/en14133849"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1016\/j.biortech.2013.04.117","article-title":"Invasive Plants as Feedstock for Biochar and Bioenergy Production","volume":"140","author":"Liao","year":"2013","journal-title":"Bioresour. Technol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1007\/s00107-021-01779-y","article-title":"Engineered Invasive Plant Cellulose Fibers as Resources for Papermaking","volume":"80","author":"Kapun","year":"2022","journal-title":"Eur. J. Wood Wood Prod."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1128\/MMBR.66.3.506-577.2002","article-title":"Microbial Cellulose Utilization: Fundamentals and Biotechnology","volume":"66","author":"Lynd","year":"2002","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1264\/jsme2.23.118","article-title":"Syntrophic Acetate-Oxidizing Microbes in Methanogenic Environments","volume":"23","author":"Hattori","year":"2008","journal-title":"Microbes Environ."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"R727","DOI":"10.1016\/j.cub.2018.05.021","article-title":"Methanogenesis","volume":"28","author":"Lyu","year":"2018","journal-title":"Curr. Biol."},{"key":"ref_29","first-page":"126","article-title":"Anaerobic Co-Digestion of Sewage Sludge and Solidago canadensis L. with Different Volatile Solid Ratios in Feedstock","volume":"37","author":"Wang","year":"2014","journal-title":"Environ. Sci. Technol. China"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Kushkevych, I., Cejnar, J., Treml, J., Dordevi\u0107, D., Kollar, P., and V\u00edt\u011bzov\u00e1, M. (2020). Recent Advances in Metabolic Pathways of Sulfate Reduction in Intestinal Bacteria. Cells, 9.","DOI":"10.3390\/cells9030698"},{"key":"ref_31","unstructured":"Gentry, T.J., Fuhrmann, J.J., and Zuberer, D.A. (2021). 18\u2014Microbial transformations of sulfur in soil. Principles and Applications of Soil Microbiology, Elsevier. [3rd ed.]."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Stams, A., and Sousa, D. (2019). Ecophysiology of acetoclastic methanogens. Biogenesis of Hydrocarbons. Handbook of Hydrocarbon and Lipid Microbiology, Springer.","DOI":"10.1007\/978-3-319-78108-2_21"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"126219","DOI":"10.1016\/j.biortech.2021.126219","article-title":"Enrichment of the Hydrogenotrophic Methanogens for, in-Situ Biogas up-Gradation by Recirculation of Gases and Supply of Hydrogen in Methanogenic Reactor","volume":"345","author":"Khan","year":"2022","journal-title":"Bioresour. Technol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"105923","DOI":"10.1016\/j.envint.2020.105923","article-title":"Symbiosis of Sulfate-Reducing Bacteria and Methanogenic Archaea in Sewer Systems","volume":"143","author":"Shi","year":"2020","journal-title":"Environ. Int."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1016\/j.ecoenv.2019.02.019","article-title":"Chemical Speciation, Pollution and Ecological Risk of Toxic Metals in Readily Washed off Road Dust in a Megacity (Nanjing), China","volume":"173","author":"Liu","year":"2019","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1283","DOI":"10.1007\/s11676-019-00921-0","article-title":"Physico-Chemical Characteristics and Heavy Metal Concentrations of Copper Mine Wastes in Zambia: Implications for Pollution Risk and Restoration","volume":"31","author":"Chileshe","year":"2020","journal-title":"J. For. Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1016\/j.envpol.2018.02.053","article-title":"Presence, Mobility and Bioavailability of Toxic Metal (Oids) in Soil, Vegetation and Water around a Pb-Sb Recycling Factory (Barcelona, Spain)","volume":"237","author":"Mykolenko","year":"2018","journal-title":"Environ. Pollut."},{"key":"ref_38","first-page":"9248123","article-title":"Ecotoxicological and Interactive Effects of Copper and Chromium on Physiochemical, Ultrastructural, and Molecular Profiling in Brassica napus L.","volume":"2018","author":"Li","year":"2018","journal-title":"BioMed Res. Int."},{"key":"ref_39","first-page":"44","article-title":"Draft Whole Genome Sequence for Four Highly Copper Resistant Soil Isolates Pseudomonas lactis Strain UKR1, Pseudomonas panacis Strain UKR2, and Pseudomonas veronii Strains UKR3 and UKR4","volume":"1","author":"Havryliuk","year":"2020","journal-title":"Curr. Res. Microb. Sci."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1393","DOI":"10.4141\/cjps80-194","article-title":"The Biology of Canadian Weeds.: 45. Solidago canadensis L.","volume":"60","author":"Werner","year":"1980","journal-title":"Can. J. Plant Sci."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"16073","DOI":"10.1038\/s41598-018-34218-9","article-title":"Simulated Warming Enhances Biological Invasion of Solidago Canadensis and Bidens Frondosa by Increasing Reproductive Investment and Altering Flowering Phenology Pattern","volume":"8","author":"Cao","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_42","first-page":"222","article-title":"An Accurate and Fast Method for Moisture Content Determination","volume":"25","author":"Fridh","year":"2014","journal-title":"Int. J. For. Eng."},{"key":"ref_43","first-page":"50","article-title":"A Method of Determining the Antioxidative Activity of Biological Matter","volume":"57","author":"Semenov","year":"1985","journal-title":"Ukr. Biokhimicheskii Zhurnal 1978"},{"key":"ref_44","unstructured":"Wrolstad, R.E., Acree, T.E., Decker, E.A., Penner, M.H., Reid, D.S., Schwartz, S.J., Shoemaker, C.F., Smith, D.M., and Sporns, P. (2005). Handbook of Food Analytical Chemistry, Volume 1: Water, Proteins, Enzymes, Lipids, and Carbohydrates, John Wiley & Sons."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1776","DOI":"10.1007\/s12161-014-9814-x","article-title":"Evaluation of Aluminium Complexation Reaction for Flavonoid Content Assay","volume":"7","author":"Pyrzynska","year":"2014","journal-title":"Food Anal. Methods"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1016\/0043-1354(73)90090-0","article-title":"Determination of Carbohydrate in Lake Sediment by a Modified Phenol-Sulfuric Acid Method","volume":"7","author":"Liu","year":"1973","journal-title":"Water Res."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"4802","DOI":"10.1021\/es1000209","article-title":"New Sludge Pretreatment Method to Improve Methane Production in Waste Activated Sludge Digestion","volume":"44","author":"Zhang","year":"2010","journal-title":"Environ. Sci. Technol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1016\/0043-1354(94)90273-9","article-title":"Interaction between Sulfate-Reducing Bacteria and Methane-Producing Bacteria in UASB Reactors Fed with Low Strength Wastes Containing Different Levels of Sulfate","volume":"28","author":"Harada","year":"1994","journal-title":"Water Res."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1007\/s11274-020-02894-3","article-title":"The Enrichment of Anaerobic Fungi and Methanogens Showed Higher Lignocellulose Degrading and Methane Producing Ability than That of Bacteria and Methanogens","volume":"36","author":"Ma","year":"2020","journal-title":"World J. Microbiol. Biotechnol."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Li, C., Ji, Q., Fu, X., Yu, X., Ye, Z., Zhang, M., Sun, C., and Qiu, Y. (2022). Low-Cost Detection of Methane Gas in Rice Cultivation by Gas Chromatography-Flame Ionization Detector Based on Manual Injection and Split Pattern. Molecules, 27.","DOI":"10.3390\/molecules27133968"},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Kushkevych, I., Dordevi\u0107, D., Kollar, P., V\u00edt\u011bzov\u00e1, M., and Drago, L. (2019). Hydrogen Sulfide as a Toxic Product in the Small\u2013Large Intestine Axis and Its Role in IBD Development. J. Clin. Med., 8.","DOI":"10.3390\/jcm8071054"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1038\/s41579-019-0250-1","article-title":"Sulfur Metabolites in the Pelagic Ocean","volume":"17","author":"Moran","year":"2019","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"123","DOI":"10.15407\/biotech8.03.123","article-title":"Regularities of Quantitative Distribution for Fe (III)-Reducing Bacteria in Natural Ecosystems","volume":"8","author":"Govorukha","year":"2015","journal-title":"Biotechnol. Acta"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1094","DOI":"10.1021\/ed075p1094","article-title":"Basic Gas Chromatography (McNair, Harold M.; Miller, James M.)","volume":"75","author":"Acree","year":"1998","journal-title":"J. Chem. Educ."},{"key":"ref_55","first-page":"217","article-title":"Express Method for Redox Potential and PH Measuring in Microbial Cultures","volume":"18","author":"Tashyrev","year":"2014","journal-title":"Int. J. Bioautom."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1165","DOI":"10.1126\/science.793008","article-title":"Titanium (III) Citrate as a Nontoxic Oxidation-Reduction Buffering System for the Culture of Obligate Anaerobes Vertebrate Central Nervous System: Same Neurons Mediate Both Electrical and Chemical Inhibitions","volume":"194","author":"Zehnder","year":"1975","journal-title":"Science"},{"key":"ref_57","first-page":"45","article-title":"Method for Determining Organic Compound Concentration in Biological Systems by Permanganate Redox Titration","volume":"18","author":"Suslova","year":"2014","journal-title":"Int. J. Bioautom."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"93","DOI":"10.15407\/biotech8.05.093","article-title":"Copper Resistant Strain Candida Tropicalis RomCu5 Interaction with Soluble and Insoluble Copper Compounds","volume":"8","author":"Prekrasna","year":"2015","journal-title":"Biotechnol. Acta"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"461770","DOI":"10.1016\/j.chroma.2020.461770","article-title":"Selective Extraction of Bioactive Compounds from Plants Using Recent Extraction Techniques: A Review","volume":"1635","author":"Lefebvre","year":"2021","journal-title":"J. Chromatogr. A"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"880115","DOI":"10.3389\/fvets.2022.880115","article-title":"A Review: Plant Carbohydrate Types\u2014The Potential Impact on Ruminant Methane Emissions","volume":"9","author":"Sun","year":"2022","journal-title":"Front. Vet. Sci."},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Jomov\u00e1, K., Hudecova, L., Lauro, P., Simunkova, M., Alwasel, S.H., Alhazza, I.M., and Valko, M. (2019). A Switch between Antioxidant and Prooxidant Properties of the Phenolic Compounds Myricetin, Morin, 3\u2032,4\u2032-Dihydroxyflavone, Taxifolin and 4-Hydroxy-Coumarin in the Presence of Copper(II) Ions: A Spectroscopic, Absorption Titration and DNA Damage Study. Molecules, 24.","DOI":"10.3390\/molecules24234335"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"106127","DOI":"10.1016\/j.biombioe.2021.106127","article-title":"Biogas: Potential, Challenges, and Perspectives in a Changing China","volume":"150","author":"Lu","year":"2021","journal-title":"Biomass Bioenergy"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"587414","DOI":"10.3389\/fpls.2020.587414","article-title":"Introduced Populations of an Invasive Tree Have Higher Soluble Sugars but Lower Starch and Cellulose","volume":"11","author":"Li","year":"2020","journal-title":"Front. Plant Sci."},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Peter, A., \u017dlabur, J.\u0160., \u0160uri\u0107, J., Vo\u0107a, S., Purgar, D.D., Pezo, L., and Vo\u0107a, N. (2021). Invasive Plant Species Biomass\u2014Evaluation of Functional Value. Molecules, 26.","DOI":"10.3390\/molecules26133814"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"3075","DOI":"10.15376\/biores.16.2.3075-3095","article-title":"Utilisation Potentials of Invasive Plants in the Owabi Dam in the Ashanti Region of Ghana","volume":"16","author":"Banunle","year":"2021","journal-title":"BioResources"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1111\/j.1744-7909.2010.01024.x","article-title":"Two-stage Hydrolysis of Invasive Algal Feedstock for Ethanol Fermentation F","volume":"53","author":"Wang","year":"2011","journal-title":"J. Integr. Plant Biol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1111\/j.1757-1707.2010.01063.x","article-title":"Biofuels and Invasive Species from an African Perspective\u2013a Review","volume":"2","author":"Witt","year":"2010","journal-title":"Gcb Bioenergy"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.ecoleng.2017.10.001","article-title":"Characteristics and Influencing Factors of Cadmium Biosorption by the Stem Powder of the Invasive Plant Species Solidago canadensis","volume":"121","author":"Zhang","year":"2018","journal-title":"Ecol. Eng."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/j.energy.2014.09.013","article-title":"Optimization of Anaerobic Co-Digestion of Solidago canadensis L. Biomass and Cattle Slurry","volume":"78","author":"Yao","year":"2014","journal-title":"Energy"},{"key":"ref_70","first-page":"16421648","article-title":"Suitability of Common Nettle (Urtica dioica) and Canadian Goldenrod (Solidago canadensis) for Methane Production","volume":"16","author":"Dubrovskis","year":"2018","journal-title":"Agron. Res."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"936","DOI":"10.1016\/j.matpr.2020.05.076","article-title":"Heavy Metal Precipitation from Sulfide Produced from Anaerobic Sulfidogenic Reactor","volume":"32","author":"Tyagi","year":"2020","journal-title":"Mater. Today Proc."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"218","DOI":"10.15421\/011833","article-title":"Sulfidogenic and Metal Reducing Activities of Desulfuromonas Genus Bacteria under the Influence of Copper Chloride","volume":"26","author":"Hnatush","year":"2018","journal-title":"Biosyst. Divers."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1139\/m87-093","article-title":"Inhibition of Methanogenesis in Pure Cultures by Ammonia, Fatty Acids, and Heavy Metals, and Protection against Heavy Metal Toxicity by Sewage Sludge","volume":"33","author":"Jarrell","year":"1987","journal-title":"Can. J. Microbiol."}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/12\/1\/198\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T17:56:48Z","timestamp":1760119008000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/12\/1\/198"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,1,3]]},"references-count":73,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2023,1]]}},"alternative-id":["plants12010198"],"URL":"https:\/\/doi.org\/10.3390\/plants12010198","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,1,3]]}}}